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Citations for New England Biolabs :
51 - 81 of 81 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The solution was split into two halves and mRNAs were isolated from each using 150 μL magnetic Oligo(dT)25 beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:100 10 mg/mL BSA in distilled water) with 0.75 µL (150 U) micrococcal nuclease enzyme solution (1:10 micrococcal nuclease (NEB, USA) in micrococcal nuclease reaction buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 75 - 150 ng of hybridized PCR products were digested with T7 endonuclease (New England Biolabs, Catalogue # M0302S) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng DNase I treated total RNA or mRNA of one technical replicate was fragmentated to ~150 nt by magnesium RNA fragmentation buffer (New England Biolabs) for 4 min at 94°C ...
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bioRxiv - Biochemistry 2024Quote: ... 4SU-labeled RNA (100 µg) was biotinylated with HPDP-biotin (cat. 21341, ThermoScientific) and captured on 150 µL of streptavidin magnetic beads (NEB) per RNA sample ...
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bioRxiv - Cell Biology 2023Quote: ... To avoid contamination of nascent RNA still attached to mtDNA into the higher molecular weight sucrose gradient fractions we digested all DNA by 150 units of DNAse I (NEB) in the presence of superaseIn (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... The ER-Halo-GCaMP6-150 was PCR amplified and the resulting fragment was combined with the hDLXI56i backbone with HiFi (NEB). The hDLXI56i enhancer was originally obtained from CN1851-rAAV-hI56i-minBglobin-iCre-4×2C-WPRE3-BGHpA (Graybuck et al. ...
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bioRxiv - Immunology 2023Quote: ... Completed libraries were then sequenced to an average depth of approximately 20M reads per sample on a partial lane of the NovaSeq6000 S4 XP flow cell using 2×150 paired-end reads with 10-base dual indexes (CAS: E6440S, NEB). After demultiplexing these samples ...
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bioRxiv - Physiology 2024Quote: ... The libraries for the Illumina sequencer (paired-end 150 bp) were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... After washing samples were resuspended in 150 µl RNA cleanup binding buffer of the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were incubated for 5 min at RT on a rotating wheel ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was stored short-term at −20°C or long-term at −150°C or immediately used in a retrotranscription reaction (ProtoScript® AMV First Strand cDNA Synthesis Kit, New England Biolabs or SuperScript® III First-Strand Synthesis System ...
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bioRxiv - Genomics 2022Quote: ... Proximity barcode ligations were carried out in 150 µl T4 ligation reaction mix (11 µL T4 ligase (NEB cat# M0437B-BM), 75 mM Tris pH 7.5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Filled-in chromatin was then ligated by adding 970 μl of Ligation Master Mix (150 μl of 10x NEB T4 DNA ligase buffer with ATP (NEB, B0202), 125 μl of 10% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... Individually barcoded strand-specific libraries for mRNA sequencing were prepared from total RNA samples of high quality (approximately 150 ng per sample) using the NEBNext® RNA Ultra II Directional RNA Library Prep Kit (New England Biolabs) for 12 PCR cycles on the liquid handler Biomek i7 (Beckman Coulter GmbH ...
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bioRxiv - Biochemistry 2024Quote: 27.4 μg of total RNA was fragmented into ∼150-200 nt fragments at 94 °C for 5 minutes using the magnesium RNA fragmentation buffer (NEB, E6186AVIAL), followed by purification with OCC ...
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bioRxiv - Genomics 2024Quote: ... genomic sites of interest were amplified from 150 ng of genomic DNA using Q5 PCR Master Mix (New England Biolabs #M0544S) with the primers containing Illumina forward and reverse adapters (listed in Table S1) ...
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bioRxiv - Immunology 2021Quote: ... It was then linearized for transfection by digesting 150 μg of DNA with 3,000 units PvuI-HF restriction enzyme (New England Biolabs, Ipswich, MA, USA), precipitated ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA-seq libraries were prepared from 150 ng total RNA using NEBNext® Ultra™ II Directional RNA library Prep Kit for Illumina (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: For Southern blot analysis 150 ng of mouse DNA was digested with 10 units of BamHI-HF (NEB, Figure 2 and 5), NdeI (NEB ...
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bioRxiv - Microbiology 2020Quote: DNA fragments (150–250 bp) were selected for library construction and sequencing libraries prepared using the NEBNext ChIP-Seq Kit (NEB, catalog#: E6200). The libraries were sequenced using the HiSeq XTen (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 150-ng DNA from each sample with or without 2 units of Phi29 polymerase (New England Biolabs, Cat# M0269L) in 20-μl total reaction volume and incubated at 30 °C for 8 hours ...
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bioRxiv - Synthetic Biology 2020Quote: PCR amplicons were randomly fragmented to an average size of 150-200 bp using NEBNext dsDNA Fragmentase (New England Biolabs, cat. M0348S), following manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: Library preparation was performed with 150 ng of starting material using NEBNext Fast DNA Library Prep Set for Ion Torrent (New England Biolabs, Massachusetts, USA) and following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: PCR products (150 – 200 ng) were digested for 30 min at 37°C in 20 ul 1X restriction enzyme buffer (NEB buffer v3.1) containing 500 ng Cas9 (Alt-R® S.p ...
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bioRxiv - Cell Biology 2023Quote: ... BarSeq PCR was performed using 200 ng of genomic DNA template in 150-μL reaction volumes with Q5 polymerase (with enhancer) (New England Biolabs, Ipswich, MA), 20 μM universal forward P5 primer ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to an input of 150 ng before preparation with a NEBNext® Ultra™ II RNA Library Prep (Magnetic Bead) kit (New England Biolabs). The polyA mRNA protocol was used for all preps ...
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bioRxiv - Genomics 2022Quote: ... we amplified a 150 bp sequence using primers pGL3-CDC20_F and pGL3-CDC20_R (Phusion High-Fidelity PCR Master Mix, NEB M0531, Supplemental Table 5) that added restriction sites for SacI and XhoI to the 150 bp sequence ...
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bioRxiv - Immunology 2023Quote: ... 150 ng of total RNA were purified using the NEBNext poly(A) mRNA Magnetic Isolation module (New Englands Biolabs Inc., Ipswich, MA, USA) and used as a template for cDNA synthesis by reverse transcriptase with random primers ...
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bioRxiv - Plant Biology 2022Quote: ... A total of 0.5 μg of total RNA per biological replicate were used for preparing 150 bp paired-end (PE) read libraries using the NEBNext®Ultra™ II RNA Library Prep Kit (New England Biolabs, Inc.). Small RNA was extracted using a Plant miRNA kit (Omega Bio-tek Inc.) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we prepared a 150 bp paired-end library using the NEBNext Ultra II DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). We checked qualities and quantities of libraries using a High Sensitivity D1000 ScreenTape on an Agilent TapeStation (Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: mRNA was isolated from up to 150 ng total RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Cat. No. E7490), and libraries were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs ...