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Citations for New England Biolabs :
1 - 50 of 81 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: 150 μl amylose resin (NEB) was equilibrated in pull-down buffer (1x PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 150 μM S-adenosyl-methionine (NEB)) ...
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bioRxiv - Cell Biology 2022Quote: ... Add 150 μL of Ligation mixture (New England BioLabs M0202L) (110 μL NFW ...
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bioRxiv - Pathology 2020Quote: ... SpeI restriction enzyme (5U per 150 μL, New England Biolabs) was used [26] and the electrophoresis conditions comprised switch times of 2 – 6 s at 15 °C and 200 V for 37 h.
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bioRxiv - Cell Biology 2022Quote: ... Add 150 μL of dA-Tailing mix (New England BioLabs E6053L) (131 μL NFW ...
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bioRxiv - Cell Biology 2022Quote: ... Add 150 μL of Quick Blunting Kit (New England BioLabs E1201L) (115.5 μL NFW 15 μL 10x blunting buffer ...
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bioRxiv - Genomics 2021Quote: ... and 150 μg chromatin was immunoprecipitated using 3 μg H3K18la (PTM Biolabs) overnight at 4°C with gentle rotation (20 rpm ...
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bioRxiv - Microbiology 2022Quote: ... 150 µl Proteinase K (800 units ml-1; New England Biolabs #P8107S), 138 µl 20% SDS ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was digested to 150-300 bp by micrococcal nuclease (NEB; M0247S). Sonicate cells in EP tubes with power output 100 W ...
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bioRxiv - Molecular Biology 2024Quote: ... we performed a streptavidin extraction (New England Biolabs, 150 μl per sample) for 30 minutes at room temperature using streptavidin-linked magnetic beads ...
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bioRxiv - Developmental Biology 2022Quote: ... containing DpnII buffer to 1x final concentration and 150 units DpnII (NEB, R0543). The digestion was incubated overnight at 37C with gentle rocking ...
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bioRxiv - Genomics 2021Quote: ... we removed fragments <150 bp using 1x AMPure® XP beads (NEB, UK). DNA quantification and quality control was performed using a BioAnalyser Instrument (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: Nucleosome arrays (∼150 ng) were digested with either 50 units of BamHI (NEB) for 1 h or 10 units of PstI (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Precipitated proteins were resuspended in 150 μL of Glycoprotein Denaturing Buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA) with or without Short Cut RNase III (New England Biolabs; 1:150) for 90 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with a 1:150 dilution of RNase III (New England Biolabs) in low-salt buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Microbiology 2024Quote: ... The culture supernatants (150 μL) were treated with DNase I (NEB, Ipswich, MA, U.S.A.) to obtain enveloped and encapsidated viral genomes ...
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bioRxiv - Biochemistry 2021Quote: ... or Endoglycosidase H (translation products of ∼50-150 kDa protein substrates; New England Biolabs, P0702S). All samples were solubilised for 12 h at 37°C and then sonicated prior to resolution by SDS-PAGE (10% or 16% PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... 80 ng genomic DNA was digested overnight with 150 units of MspI (New England Biolabs) at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... consisting of 150 μL 10X NEB T4 DNA ligase buffer with 10 mM ATP (NEB), 125 μL 10% Triton X-100 ...
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bioRxiv - Molecular Biology 2023Quote: ... For AsiSI digestion, samples were equilibrated (2 min, RT) in 150 µL CutSmart buffer (NEB). 3µl recombinant AsiSI endonuclease (10U/µL ...
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bioRxiv - Molecular Biology 2022Quote: ... then RNA was fragmentated to ~150 nt by magnesium RNA fragmentation buffer (New England Biolabs) for 4 min at 94°C ...
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bioRxiv - Genomics 2022Quote: ... were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB, #E6047A) in Quick Ligation Reaction Buffer (NEB ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions of 150 µl were set using a high-fidelity polymerase (Phusion, New England Biolabs). Each template PCR was verified by agarose gel electrophoresis and then purified to use for yeast transformation.
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bioRxiv - Microbiology 2020Quote: ... CFF1 and ~150 bp upstream and downstream were cloned into the XhoI and XbaI (New England Biolabs) sites of pRS416 using standard cloning procedures ...
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bioRxiv - Immunology 2021Quote: ... Add 948 μl of ligation master mix: 150 μl 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μl 10% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated for 90 min with a 1:150 dilution of RNase III (New England Biolabs) in low-salt buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 ng of deaminated DNAs were digested to nucleosides with the Nucleoside Digestion Mix (NEB, Ipswich, MA) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: 150 ng of total RNA was applied to the NEBNext rRNA Depletion Kit (New England Biolabs, #E6310L) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... 150-500 ng of DNA were incubated with 7.5 U phi29 (<129) DNA polymerase (New England Biolabs) in presence of dATP ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was incubated with 150 μl of oligo (dT)25 cellulose beads (New England BioLabs, cat no. S1408S) for 15 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The medium was then exchanged for 150 μL 10 nM SNAP-Dy549 (SNAP-Surface 549, New England Biolabs) in complete medium and incubated for a further 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were reinjected at 75 μl/h and co-flowed with 150 μl/h RT mix (1.65x Maxima H-RT Buffer, 0.033 U/μl USER II (NEB), 15 U/μl Maxima H-RT (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... were reinjected at 75 μl/h and co-flowed with 150 μl/h PCR mix (1.65x NEBNext Ultra II Q5 Master Mix, 0.033 U/μl USER II (NEB), 1.32 M Propylene glycol ...
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bioRxiv - Molecular Biology 2023Quote: 150 ng of genomic DNA extracted from ear punch biopsies (see Genotyping protocol) was digested with MseI (NEB) and 1x CutSmart buffer at 37°C for two hours ...
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bioRxiv - Genomics 2024Quote: ... a 300 µL PCR reaction was prepared with 150 µL of NEBNext High-Fidelity 2X Master Mix (NEB), 100 µL of SPRI purified messenger products ...
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Genome Assembly of the Roundjaw Bonefish (Albula glossodonta), a Vulnerable Circumtropical SportfishbioRxiv - Genomics 2021Quote: ... We carried out double digests of 150 – 200 ng total DNA per sample using the two enzymes in the manufacturer’s supplied buffer (New England Biolabs) for 8 hours at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was incubated with 150 μl of oligo (dT)25 cellulose beads (New England BioLabs, cat no. S1408S) for 15 min at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Filled-in chromatin was then ligated by adding 970 μl of Ligation Master Mix (150 μl of 10x NEB T4 DNA ligase buffer with ATP (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... A-tailed fragments were ligated to 50 nL of 5 mM T7 promoter containing adapters(21) with cell barcodes and UMI with 150 nL ligation mix (25 nL T4 DNA ligase (400.000U, NEB), 3.5 nL MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed in CutSmart buffer, and incubated (1h, RT) in 150 µL blunting mix (112.5 µL ddH2O, 15 µL 10x blunting buffer, NEB, 15 µL 100 µM dNTPs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and gel excision of DNA between 150 bp and 1,500 bp was performed using the Monarch DNA Gel Excision Kit (NEB). Concentrations of the purified libraries were measured using PicoGreen (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... The next day samples were washed twice with dig-wash (wash buffer with 0.025% digitonin) and then resuspended in 150 µl dig-wash containing pA-MNase (New England Biolabs 700 ng/ml ...
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bioRxiv - Microbiology 2023Quote: cDNA was generated starting from 150 ng of RNA using ProtoScript® II First Strand cDNA Synthesis Kit (NEB) using random hexamers in agreement with the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... sequencing pools were in-house single-end sequenced (150 bp) on an Illumina NextSeq500 (New England Biolabs, Ipswich, MA) at the University of Guam Marine Laboratory ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated for 30 minutes shaking at 1.500 rpm at room temperature with protein extract (500 µg of whole cell protein extract or 150 µg of CPE) and murine RNase Inhibitor (NEB) in the corresponding protein extraction buffer (without glycerol) ...
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bioRxiv - Neuroscience 2020Quote: ... a gel slice corresponding to 80 to 150 nt was isolated (based on size markers in an adjacent lane: low range ssRNA ladder, NEB). The reverse transcribed product in the gel slice was eluted ...
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bioRxiv - Molecular Biology 2020Quote: ... The converted RNA was fragmented into 150 - 200 nt fragments by incubation at 94 °C for 8 minutes in fragmentation buffer (NEB). The fragmented RNA was then used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit ...
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bioRxiv - Cell Biology 2021Quote: ... 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB) and incubated at 50°C for 1 hour to form the new pCDH-TagBFP-T2A-myc-BirA*-Tensin3 construct ...
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bioRxiv - Microbiology 2020Quote: ... In the meantime, we prepared 150 μl of T4 PNK mix (120 μl H2O, 20 μl 10x T4 PNK buffer (NEB), 1 μl Murine RNase Inhibitor (NEB) ...