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Citations for New England Biolabs :
51 - 100 of 3408 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... The 2-kpb cbln12 promoter (Dohaku et al., 2019) and lTl-Kaede-pAS were subcloned to pT2ALR-Dest by NEBuilder (NEB, USA). To generate Tg(5xUAS-hsp70l:HA-skor2-P2A-mCherry ...
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bioRxiv - Cell Biology 2022Quote: ... amplicons were inserted into BsmBI-linearized lentiGuide-Puro vector by Gibson Assembly (NEB). Then ...
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bioRxiv - Genomics 2020Quote: The vector backbone (lentiGuide-Puro, Plasmid no. 52963) was digested with Esp3I (NEB) and treated with rSAP (NEB ...
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bioRxiv - Genomics 2023Quote: ... The CROP-seq-opti-Puro-T2A-GFP was digested by Esp3I (NEB R0734L) at 37 °C for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... Sequences of DNMT3B transcripts were isolated from pcDNA4-DNMT3B1 and pcDNA4-DNMT3B3ΔEx5 plasmids33 by PCR and assembled with pLenti construct using Gibson assembly (New England Biolabs) following the manufacturer protocol.
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bioRxiv - Molecular Biology 2024Quote: pLV-CMV-SNAPtag-DasherGFP was transformed into NEB Stable Competent E.coli (New England Biolabs; Massachusetts, USA) and a single colony was inoculated at 30 °C in 200 mL Terrific Broth ...
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bioRxiv - Cancer Biology 2021Quote: ... vector was generated by replacing the BleoR cassette with Cre recombinase from the pBS513 EF1alpha-Cre vector in the plenti sgRNA(MS2)_zeo backbone using PCR and HiFi assembly (NEB). One BsmBI site in the Cre cassette was destroyed through targeted mutagenesis ...
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bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with NdeI (NEB) and AfeI (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with BstBI (NEB) and AfeI (NEB) ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... pLV-EF1a-IRES-Puro LIC-compatible vectors were digested with SrfI (New England Biolabs). PCR fragments and SrfI-digested vector were combined with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs #E2621 ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng of pLenti6/V5-DEST™ vector was used as template per reaction with Q5® High-Fidelity DNA Polymerase (NEB, #M0491S). The PCR program ...
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bioRxiv - Cancer Biology 2023Quote: ... The EGFP sequence was integrated into pLenti6-V5/DEST at the SpeI-SacII site using Gibson Assembly (New England Biolabs, Ipswich, MA, USA). These lentiviral vectors were infected to cell lines as previously described 10 ...
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bioRxiv - Systems Biology 2022Quote: ... JunDN-HA-T2A-GFP was amplified from the pLVX-CMV backbone using Phusion Mastermix (NEB, catalog # M0531), and inserted into the empty backbone of pAK_Tol2_TRE_Puro (Addgene ...
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bioRxiv - Genomics 2021Quote: ... the CMV enhancer/promoter region was amplified from the pcDNA3.0 backbone using Phusion High-Fidelity Polymerase (NEB) and subcloned into the pUC19 vector upstream of the circEfnb2 or circMini backsplicing cassettes ...
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bioRxiv - Neuroscience 2024Quote: ... was used to replace the CMV promoter in pAAV.U6.shRLuc.CMV.ZsGreen.SV40 (Penn Core Vector) plasmid by excising out the CMV promoter using the EcoRI (NEB #R3101) and NHE-I (NEB #R3131 ...
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bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with BsrGI-HF (NEB) and EcoRI-HF (NEB) ...
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bioRxiv - Genomics 2020Quote: ... LentiGuide-Puro linearized vector and annealed oligos were ligated using Quick Ligase (New England BioLabs) for 20min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... We digested the Lenti-gRNA-Puro plasmid with BsmBI restriction enzyme (New England Biolabs, NEB) for 12 h at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... We digested the Lenti-gRNA-Puro plasmid with BsmBI restriction enzyme (New England Biolabs, NEB) for 12 h at 55 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The construct AAVS-Puro-CAG-fl-STOP-fl-Cas9 was linearized with MluI (NEB, R3198S) and FseI (NEB ...
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bioRxiv - Genomics 2022Quote: pU6-sgRNA EF1Alpha-puro-T2A-BFP (52) was digested with BstXI (New England Biolabs, R0113S) and BlpI (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... All ngRNAs were cloned into pLKO.5 Puro-2A-RFP between BsmBI (New England Biolabs) cut sites ...
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bioRxiv - Molecular Biology 2022Quote: Bclx and CD44-v5 minigenes were cloned in EGFP-C1 plasmid following the CMV promoter using ExoIII (NEB) enzyme following manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and introduced by In Fusion cloning into pDual_dsCas9_Venus/BFP/Puro/Blast linearized with SbfI (NEB, #R0642S) or BsmBI (NEB ...
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bioRxiv - Genomics 2023Quote: ... Lenti-epeg-Puro-P2A-EGFP was digested with BsmBI-v2 (New England Biolabs, Cat. No. R0739S) to create a single cut and produce a linearized backbone vector of 9007 base pairs ...
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bioRxiv - Genetics 2024Quote: ... and then ligated into the targeting plasmid px333-puro at the BbsI or BsaI (NEB, USA) restriction enzyme sites ...
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bioRxiv - Physiology 2022Quote: ... The scAAV-CMV-GFP plasmid was digested with EcoRI and HpaI (New England Biolabs; Cat. Nos. R3101 and R0105) and the linearized scAAV-CMV plasmid without the GFP was gel purified ...
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bioRxiv - Synthetic Biology 2023Quote: ... To generate standard curves for absolute qPCR we linearized pTransgene (p8-pAAV2.1 CMV eGFP3)37 with XhoI and NheI enzymes (NEB) and diluted in PCR-grade water from 2.5× 10^8 to 25 copies/μL in 10-fold serial dilutions ...
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bioRxiv - Genomics 2021Quote: Amplicons and pLCv2-opti-stuffer-dsRed-puro were digested with XbaI and BamHI HF (New England Biolabs) and purified ...
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bioRxiv - Molecular Biology 2021Quote: ... The pLVX-EF1alpha-SARS-CoV-2-Nsp1/Nsp2-2XStrep-IRES-Puro plasmids were digested with BamHI (NEB) and MluI (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Immunology 2021Quote: VH and VL sequences of candidate sequences were cloned into a pcDNA.3 based vector with dual CMV promotor harboring IgG1 heavy chain and light chain backbone using NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Molecular Biology 2020Quote: ... and the CMV promoter was removed to prevent aberrant transcription by digesting the plasmid with ClaI-HF and BamHI-HF (NEB), gel extracting ...
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bioRxiv - Cancer Biology 2021Quote: ... vector was generated by replacing the Ef1a promoter with a CMV promoter cassette in pLV-Ef1a-Cre-U6-sgRNA(MS2) using PCR and HiFi assembly (NEB). The pLV-CMV-CreERT2-P2A-[puroR/zeoR/hygR]-U6-sgRNA(MS2 ...
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bioRxiv - Neuroscience 2021Quote: ... and the product was inserted into a lentiviral expression vector (pSIH-H1) containing CMV promotor using NEBbuilder HIFI kit (NEW ENGLAND Biolabs). To co-express GFP-actin and mCherry-KDEL ...
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bioRxiv - Microbiology 2021Quote: ... The fragment of sNTurboID was PCR amplified from pLX304 CMV FKBP-V5-sTurboID (N) and cloned into the pcDNA5-VP35-HA using NEBuilder HiFi DNA Assembly Master Mix (NEB). The fragment of VP35-sNTurboID ...
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bioRxiv - Cell Biology 2024Quote: ... or N-terminus of PEmax (PEmax-hP53DD-P2A-hMLH1dn) under the constitutive expression from a CMV promoter using Hifi DNA assembly (NEB). To generate the other two all-in-one prime editor with linkages in between the three components ...
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bioRxiv - Plant Biology 2023Quote: ... A cDNA clone for the 2b protein of Ho-CMV was recapitulated by site-directed mutagenesis of the coding sequence of the Fny-CMV 2b protein using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). The resultant DNA product encoding the recapitulated Ho-CMV 2b protein contained the amino acid substitutions S47A ...
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bioRxiv - Cell Biology 2023Quote: ... flag tag and SunTag reporter were cloned from the SINAPs construct to replace the CMV promoter and tdTomato reporter of the tdTomato-FL2 plasmid using NEBuilder HiFi DNA Assembly technology (NEB).
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bioRxiv - Neuroscience 2023Quote: ... the CMV PSAM4 GlyR WPRE BGH pA plasmid was subjected to an overnight restriction digestion reaction using BsaI-HF v2 (NEB). The restriction reaction products were run on a gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... sequences were cloned into a human U6 (hU6)-driven expression plasmid that also contains a CMV-driven GFP transfection reporter using HiFi DNA Assembly (New England Biolabs). All plasmids were sequence-verified by whole plasmid Nanopore sequencing (Primordium ...
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bioRxiv - Microbiology 2024Quote: ... The mutagenized E3/E2 region and the pCHIKV-CMV-mKate plasmid (with two subsequent stop codon mutations in E2) were digested with ApaI and XhoI (NEB). Following dephosphorylation using alkaline phosphatase (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... sequences were cloned into a human U6 (hU6)-driven expression plasmid that also contains a CMV-driven GFP transfection reporter using HiFi DNA Assembly (New England Biolabs). All plasmids were sequence-verified by whole plasmid Nanopore sequencing (Primordium ...
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bioRxiv - Cell Biology 2022Quote: ... pWPI Puro was digested by PmeI and dephosphorylated using 20 U of calf alkaline phosphatase (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Genetics 2024Quote: ... and then the oligos were cloned into the digested lentiGuide-Puro plasmid using Quick Ligase (NEB cat# M2200S). This assembly was then transformed using One Shot Stbl3 Chemically Competent E ...
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bioRxiv - Cell Biology 2021Quote: ... Amplified cDNA fragments and HindIII-digested pFLAG-CMV-6a were subjected to the In-Fusion or NEBuilder reaction (New England BioLabs, E2621).
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bioRxiv - Genomics 2021Quote: ... Produced insert was cloned into the abovementioned pENTR vector from Twist Biosciences bearing GSH1 and GSH2 300bp homology arms as well as CMV promoter sequence using Gibson Assembly Master Mix (NEB, #E2611L). HDR donors were amplified from these plasmids using biotinylated primers with phosphorothioate bonds between the first 5 nucleotides on both 5’ and 3’ ends ...
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bioRxiv - Synthetic Biology 2022Quote: The PCR insert was assembled into 1600 ng of a linearized mRNA selection vector (AAV9-CMV-Express) with NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) at a 3:1 insert:vector Molar ratio in a 80 μL reaction volume ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmids for overexpressing the long and short isoforms of HERPUD2 were generated by cloning corresponding sequences into p3×Flag-CMV between the EcoRI and BamHI (NEB, R3136S) restriction sites ...