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Citations for New England Biolabs :
1 - 50 of 3408 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced into either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced to either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced to either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... The SETD3 V266D mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs). The SETD3 V266D construct was then used as a template to sequentially incorporate Q257A and Y288P mutations by Phusion site-directed mutagenesis using the following primer pairs ...
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bioRxiv - Biophysics 2022Quote: ... This SETD3 mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs).
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bioRxiv - Biophysics 2022Quote: ... The SETD3 G361R mutant was introduced into EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) by Gibson Assembly (New England Biolabs). The SETD3 G361R construct was then used as a template to incorporate F409A or F409A and N413A mutations by Phusion site-directed mutagenesis using the following primer pairs ...
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bioRxiv - Developmental Biology 2020Quote: ... pLenti CMV-GFP-Puro was digested with BamHI and SalI (NEB) to remove GFP cassette and served as the vector backbone ...
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bioRxiv - Cancer Biology 2021Quote: pLenti CMV Hygro DEST (w117-1) was digested with SalI and BamHI (New England BioLabs Inc., MA) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: The pLenti-mCherry-Puro vector was constructed using the HiFi assembly kit (#E2621S, NEB) with an mCherry-T2A-PuroR (puromycin resistant gene ...
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bioRxiv - Molecular Biology 2024Quote: ... The pLenti-CRISPRv2-Guide-Puro vector was digested with BsmBI (New England Biolabs, R0739L) and NheI (Takara ...
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bioRxiv - Microbiology 2023Quote: ... pLENTI-CMV-GPRC5A was mutated to remove m6A sites (A57G, G120T, C174T, A264G) using Q5 site directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti-PGK-ATP6V1H(Δ176-191)-FLAG-Puro was produced using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). M4P-EGFP-LC3B and pOGP were kind gifts from F ...
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bioRxiv - Molecular Biology 2020Quote: ... The gRNAs targeting SERPINB5 and Tnf were created by site-directed mutagenesis of pLenti-Il33gRNA6-puro using primers listed in Supplementary Table S2 and the Q5® Site-Directed Mutagenesis Kit (NEB) according to manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were subcloned into pLenti-MCS-GFP-SV40-Puro empty vector that linearized by XbaI and SpeI restriction enzymes (New England Biolabs, Inc., USA) via Gibson Assembly kit (TransGen Biotech. ...
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bioRxiv - Cell Biology 2023Quote: ... Amplicons were purified by agarose gel extraction and inserted into pLVX-CMV-MCS-10N-mPGK-puro at EcoRI and NotI sites by using NEBuilder HiFi DNA Assembly Master Mix (NEB Cat# E2621L). At least 3 individual E.coli clones were picked for plasmid extraction and plasmids were sequenced with CMV-Forward and mPGK-Reverse primers (See Key resource table ...
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bioRxiv - Cell Biology 2024Quote: ... with pAdenoX-CMV-3xHA-FHOD3L-CMV-DsRed after digesting with PacI (NEB) in Cutsmart buffer (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... We assembled the pLenti-3HA-Clic4-T2A-GFP vector with pLenti backbone and fragments using NEBuilder HiFi DNA Assembly Master Mix (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The pLenti vector was digested with BamHI (NEB #R0136) and XbaI (NEB #R0145 ...
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bioRxiv - Plant Biology 2020Quote: pGEMHE-DEST containing GmSALT3 was linearized using SphI-HF (New England BioLabs); cRNA was synthesized using mMESSAGE mMACHINE T7 Kit (Ambion) ...
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bioRxiv - Microbiology 2021Quote: ... pLenti-Cas9-GFP was digested with BsmBI (New England Biolabs). Primers listed in supplemental table 1 were annealed and ligated into the digested plasmid using T4 ligase (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: ... pLenti-GFP was digested by restriction enzymes AsiSI (NEB, R0630S) and MluI-HF (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... to pcDNA4/TO and PiggyBac-CMV-MCS-IRES-mCherry and PiggyBac-CMV-MCS-IRES-mNeongreen by PCR using Q5 High Fidelity 2X mastermix (NEB). PiggyBac-CMV-MCS-IRES-NLS-TagBFP was used as empty vector control ...
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bioRxiv - Plant Biology 2020Quote: ... pGEMHE-DEST containing TaHKT1;5-D was linearized using sbfI-HF (New England Biolabs) before synthesising cRNA using the mMESSAGE mMACHINE T7 Kit (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... and pLenti hSynapsin Cre WPRE was digested with AgeI and EcoRI (NEB). Digested plasmids were run on a 1% agarose gel and gel extracted using a standard kit (Machery Nagel) ...
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bioRxiv - Genomics 2021Quote: The amplicon and pLCv2-opti-stuffer-mCherry-puro (or -mTurq2-puro) were digested with BsrGI-HF and PmeI (New England Biolabs) and purified as above ...
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bioRxiv - Immunology 2023Quote: ... The mRuby-OAS3 CDS and mRuby2 were amplified via PCR and primers (Supplemental table 1) and inserted into pLenti-PGK-PuromycinR via Gibson assembly (New England Biolabs: E2611S). The pLenti-mRuby2-PABPC1 is described in Burke et al. ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 37°C into pPB_U6::gRNA_EF1a::BFP-Puro digested with BlpI (NEB #R0585S) and BstXI (NEB #R0113S) ...
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bioRxiv - Systems Biology 2023Quote: ... HiFi-Cas9 and LZ3-Cas9 constitutive expression plasmids lentiHiFi-Puro and lentiLZ3-Puro were constructed by the Gibson Assembly Site-Directed Mutagenesis approach (NEB, #E2621) using lentiCas9-Puro as the template ...
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bioRxiv - Synthetic Biology 2020Quote: ... ENTR and DEST vectors were generated using Gibson assembly (NEB Builder Hi-Fi DNA assembly #E2621S) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Genes of interest were cloned into the pLenti CMVTRE3G eGFP Neo (w821-1) plasmid using a Gibson Assembly Master Mix kit (Cat# E2611S, New England Biolabs, Ipswich, MA). pLenti CMV rtTA3 Blast (w756-1 ...
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bioRxiv - Biochemistry 2020Quote: ... λex = 488 nm and λem = 526 nm were used for detection of covalently bound flavin and λex = 670 nm and λem = 633 nm for visualisation of the Blue Prestained Protein Standard (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... at λex = 488 nm and λem = 526 nm for detection of covalently bound flavin and at λex = 670 nm and λem = 633 nm for visualization of the Blue Prestained Protein Standard (NEB).
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bioRxiv - Cell Biology 2023Quote: ... we first cut pHAGE-CMV-EGFP-W1 with NotI (NEB # R0189S), followed by the treatment with DNA Polymerase I ...
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bioRxiv - Genomics 2020Quote: ... LentiGuide-Puro vector was digested with BsmBI (New England BioLabs) and dephosphorylated using rSAP (New England BioLabs ...
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bioRxiv - Neuroscience 2023Quote: ... downstream of the CMV promoter using NEBuilder HiFi Assembly (New England Biolabs).
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bioRxiv - Cancer Biology 2024Quote: ... the pLenti CRISPR V2 was digested for 2 h at 50°C with Bsmb1 (NEB, R0580S) and the resulting digested fragment was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2021Quote: ... The gene fragment and Lenti_gRNA-Puro were digested using FseI (NEB) and MluI-HF (NEB) ...
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bioRxiv - Genomics 2024Quote: ... Tet-puro-pLKO plasmid was digested with AgeI and EcoRI (NEB) restriction enzymes ...
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bioRxiv - Genomics 2023Quote: ... the CROPseq-Guide-Puro vector was digested by using MluI (NEB) and SmaI (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... the pGL3-U6-sgRNA-PGK-puro was linearized with BsaI (NEB) at 37°C for 1.5h ...
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bioRxiv - Cell Biology 2020Quote: ... 64 Annealed oligonucleotides were ligated with the AgeI-HF and EcoRI-HF digested pLKO.1 puro vector (Add gene #8453) using the T4 DNA Ligase (New England Biolabs) and the resulting plasmids were transformed into E ...
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bioRxiv - Cell Biology 2024Quote: ... The ligation reaction was performed by incubating the gRNA insert and the pSpCas9(BB)-2A-Puro V2.0 vector at a ratio of 7:1 in the presence of T7 DNA ligase (New England Biolabs, # M0318S) enzyme at 25°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA fragments were combined with the digested pLenti plasmid in a Gibson Assembly reaction mix (NEB #M5510AA) at a molar ratio of 1:3:3 [vector:TurboID:UL26] for 1 hour at 50C ...
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bioRxiv - Molecular Biology 2024Quote: Omni-ATAC or CUT&Tag libraries assembled into the pLenti-STARR vector using HiFi assembly (NEB, E5520S), as previously described [Arnold et al ...
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bioRxiv - Neuroscience 2022Quote: ... starting from a pENTR3C-Raph1-WT plasmid and a pCAG-DEST-3xFLAG-2xStrep which was generated by HIFI assembly (NEB) using pCAG-EGFP (Addgene 89684) ...
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bioRxiv - Plant Biology 2022Quote: ... the UBQ10 promoter was removed from pUB-RFP-DEST through digestion with restriction endonucleases PspXI and PmeI (New England Biolabs) and the vector subsequently re-ligated using Klenow polymerase (DNA Polymerase I ...
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bioRxiv - Synthetic Biology 2024Quote: ... and cloned into CMV-driven expression plasmids using HiFi DNA Assembly (New England Biolabs). Single-guide RNA (sgRNA ...
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bioRxiv - Bioengineering 2024Quote: ... and cloned into CMV-driven expression plasmids using HiFi DNA Assembly (New England Biolabs). Single-guide RNA (sgRNA ...
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bioRxiv - Cell Biology 2021Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 was digested using BbsI-HF (NEB) at 37 °C for 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2019) and cloned into lentiviral pLenti-CAG-mCherry vector using NEBuilder HiFi DNA assembly (New England Biolabs, E2621) to generate pLenti-Ncad WT-HA-mCherry or pLenti-Ncad W161A-HA-mCherry.