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Citations for New England Biolabs :
51 - 100 of 100 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... washed once with media and released for 8 hours with 24 mM dCTP (New England Biolabs # N0441S). 2 mM of thymidine or 0.1 mg/ml of Nocodazole (Millipore-Sigma # SML1665 ...
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bioRxiv - Microbiology 2022Quote: ... Freshly prepared supercoiled plasmid DNA was then isolated from overnight cultures using a Monarch miniprep kit (NEB) and then immediately subjected to restriction digestion with T7 endonuclease (NEB ...
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bioRxiv - Biophysics 2021Quote: ... 5% CO2 -balanced complete cell culture medium with 5 μM SNAP-Surface Alexa Fluor 647 (NEB, S9136S), for 15 minutes at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Plasmid libraries were harvested from liquid culture using the Monarch® Plasmid DNA Miniprep Kit (NEB #T1010). Miniprep kit reagents were scaled to accommodate one spin column for every 5mL of culture.
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cleared bacterial lysate from 1L of culture was incubated with 2mL of equilibrated amylose resin (NEB #E8021L) in a rotator overnight at 4C ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs) and quantified using the Qubit Broad Range dsDNA kit (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... Media was directly used to detect viral RNA using Luna® Universal One-Step RT-qPCR Kit (NEB) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... gibco) supplemented with 10% fetal calf serum (eurobio scientific) and 1% ZellShield cell culture contamination preventive (Minerva-Biolabs). Cells were passaged at 80-90% confluency ...
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bioRxiv - Evolutionary Biology 2024Quote: ... keilini culture was carried out at Génome Québec using the NEB stranded mRNA library protocol (New England Biolabs) for cDNA synthesis and library preparation before sequencing 50 million reads on the NovaSeq6000 platform using 2×100 chemistry ...
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bioRxiv - Cell Biology 2020Quote: ... coli CJ236 was picked into 1 ml of 2YT media (Roth, 6676.2) supplemented with M13KO7 helper phage (NEB, N0315) to a final concentration of 1 × 108 pfu/ml and ampicillin (final concentration 100 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... each 180 μl sample/Saccharibacteria co-culture was treated with Endo Hf Kit P0703L (New England Biolabs, Evry, France): 3 μl of each reagent was added to the sample and incubated for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The holder allowed immersion of the cochlea and the middle ear in oxygenated (95 % O2, 5 % CO2) cell culture medium (Minimum Essential Medium with Earle’s balanced salts, SH30244.FS Nordic Biolabs). The bone of the bulla was removed gently with bone cutters which exposed the middle ear and the basal turn of the cochlea ...
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA and total DNA was isolated from overnight cultures using Monarch® plasmid mini-prep kit (NEB, #T1010) and Monarch® genomic DNA purification kit (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 30 min at 37°C in appropriate media containing 3µM of cell-permeable SNAP-Cell® 647-SiR (New England BioLabs). Cells were then rinsed twice with fresh media and incubated for 30min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the newly produced CENP-A-SNAP pool was labelled by exposing the mitotic cells to media containing 3 µM SNAP-Cell 647 (NEB) and 5 µM STLC (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: A 3 µL volume of parasite culture or 10 ng of plasmid was used in 25 µL PCR reactions with Phusion High-Fidelity DNA polymerase (NEB). To confirm pMG74PfAUBL insertion into the 3’ UTR of PfAUBL ...
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bioRxiv - Microbiology 2020Quote: ... TMR-Star was added to cells in culture samples inside an anaerobic chamber to a final concentration of 250 nM (New England Biolabs) and the mixture incubated for 30 minutes in the dark ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from 0.25×106 cells at day 12 of cell culture using the Monarch total RNA miniprep kit (New England Biolabs, T2010S) and quantified using the Qubit RNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to Glutathione Sepharose 4B affinity resin (1 ml bed volume per 2 l culture; New England Biolabs), and incubated with rotation for
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bioRxiv - Cell Biology 2023Quote: ... and 21-base pair (bp) barcodes (post-culture) were amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs). Primers used are listed in Supplemental Table 11 ...
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bioRxiv - Microbiology 2023Quote: ... Inserts were amplified by PCR using bacterial overnight culture or synthesized by Twist Bioscience and joined with the digested vector using Hi-Fi DNA Gibson Assembly (NEB) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue cultures that were cultivated on one filter membrane (3 cultures) were transferred as one sample into RNA Protection buffer (New England Biolabs) and RNA was isolated according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from frozen hDRG tissue and GBP- or vehicle-treated primary hDRG cultures (n = 3 per condition) using the Monarch Total RNA Miniprep Kit (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Plasmids (Supplementary Table 2) were purified from overnight bacterial cultures using the Monarch Plasmid Miniprep Kit (New England Biolabs, UK). Polymerase chain reaction was carried out using Q5 high-fidelity polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: Cells growing in monolayer cultures were directly harvested in RNA lysis buffer and processed with the Monarch RNA purification kit (NEB). cDNA was synthesized with the LunaScript RT SuperMix kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptotic cells were generated by supplementing cell culture with 10 µM staurosporine (Cayman) to induce apoptosis74 and 1 unit/mL DNAse I (New England Biolabs) to prevent cells from clumping ...
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bioRxiv - Cell Biology 2024Quote: ... Thymidine was then removed by washing cells with culture media and blocking of existing CENP-A was performed by treatment with 10 mM SNAP-Cell® Block BTP (S9106S, NEB) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in CO2-balanced pre-warmed media supplied with 1 µM O6-benzylguanine (BG)-coupled Alexa Fluor 647 (NEB, S9136S) for 15 min at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL of culture was removed and treated with DNase I (New England Biolabs; 4 µL and 25µL of DNaseI buffer) for 1 h at 30 °C to remove any extracellular DNA ...
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bioRxiv - Synthetic Biology 2021Quote: SynHox assemblon BACs were verified by digesting a ∼250-500ng purified by alkaline lysis (72) from small scale (5-10 mL) saturated bacterial culture with PvuI-HF (New England Biolabs R3150S). Digestion reactions were carried out at 37°C for 3-24 hours ...
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bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... palustris cultures at mid-exponential phase in duplicate using the Monarch® Genomic DNA Purification Kit (New England Biolabs® Inc.). Amplified PCR product using primers in Supplementary Table VI was diluted to 109 copies of amplicon/μL per the following equation.
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bioRxiv - Microbiology 2022Quote: ... 50 mL cell culture were grown in LB to stationary phase and processed with the NEB Monarch genomic DNA purification kit (NEB #T3010). For genomic DNA extraction of E ...
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bioRxiv - Microbiology 2021Quote: ... coli culture grown in LB supplemented with 100 μg/ml carbenicillin (LB+CA100) via miniprep kit (New England Biolabs, cat#T1010S). The plasmid was transformed into chemically competent E ...
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bioRxiv - Microbiology 2024Quote: ... The “co-culture” RNA sample was treated with an NEBNext® Poly(A) mRNA Magnetic Isolation kit (New England Biolabs, UK), and 100 µl of the produced supernatant (bacterial fraction ...
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bioRxiv - Microbiology 2024Quote: Three μL volumes of parasite culture were used in 25 μL PCR reactions with Phusion High-Fidelity DNA polymerase (NEB, M0530). Primer pairs and anticipated sizes of PCR products are indicated in Fig ...
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bioRxiv - Synthetic Biology 2024Quote: ... approximately 0.2µL of overnight culture was added to the reaction mixture containing 1.5µL 10x Taq ThermoPol® buffer (New England Biolabs, Cat# B9004S), 0.3µL 10mM dNTP mix (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.3 µl of this boiled culture was used as templates in 30 µl qPCR reactions using Luna® Universal qPCR Master Mix (New England Biolabs). qPCR quantification of RT-DNA abundance was performed using a set of three primers ...
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bioRxiv - Microbiology 2021Quote: ... resulting in ∼1 ml of turbid bacterial culture which was subsequently used as input for plasmid purification by miniprep kit (New England Biolabs, cat#T1010S). The pooled plasmid library DNA for each library was eluted in 35 μl of elution buffer and quantified ...
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bioRxiv - Genetics 2024Quote: DNA from mNPCs in culture were harvested from Kmt2a+/LSL cell line cell line (1 million cells) using Monarch® HMW DNA Extraction Kit (NEB, T3050). We sheared the high molecular weight (HMW ...
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bioRxiv - Cell Biology 2024Quote: For each sample 2 ml of log phase culture were harvested and total RNA extraction was performed using Monarch® Total RNA Miniprep Kit (New England Biolabs T2010S) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Transformation reactions were then transferred to deepwell 96-well plates containing room temperature NEB SOC Outgrowth Media (New England Biolabs, Ipswich, MA, USA), and incubated for 1 hour at 37°C with shaking at 800 rpm ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted from 1 ml of overnight culture with the kit Monarch HMW DNA Extraction kit (T#3060 New England Biolabs; Ipswich, MA, USA) following the manufacturer instructions for High Molecular Weight DNA extraction from gram-negative bacteria ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA (tissue samples) or 100ng RNA (cell culture experiments) using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Evolutionary Biology 2024Quote: Target amplicons were amplified by polymerase chain reaction (PCR) from the cDNA of slow swimmer and thecate cultures (Fig. S2A) with the Luna Universal qPCR Master Mix (NEB, Cat, No. M3003). The amplicons were run on a 1% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... high molecular weight (HMW) DNA was extracted from an overnight culture using the Monarch HMW DNA Extraction Kit for Tissue (New England Biolabs, Ipswich, MA, USA) as per manufacturer’s protocol for HMW DNA extracted from bacteria ...