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Citations for New England Biolabs :
1 - 50 of 100 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... each culture was recovered in provided outgrowth media (NEB) by shaking at 37°C at 250 rpm for 1.5 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and tryptic peptides were generated using Trypsin-Ultra (P8101S, NEB) following the protocol described previously2 ...
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bioRxiv - Systems Biology 2022Quote: CAM-modified tryptic digest of bovine serum albumin (New England BioLabs Inc.) was labeled with dimethyl using the in-solution labeling protocol (Boersema et al ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... N-glycans were enzymatically removed from the tryptic peptides with PNGase F (NEB) overnight at 37°C ...
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bioRxiv - Genomics 2022Quote: ... agar plugs were equilibrated 1X Buffer3.1 (NEB) for 24 h at 4°C ...
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bioRxiv - Genetics 2023Quote: ... In agar chromosome digestion with BamHI (NEB, R0136L) was used to release the entire rDNA locus (∼1.5 Mb to ∼5 Mb ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Genetics 2024Quote: ... a subset of a second identical transformation was taken directly after electroporation and split across ten 1 mL cultures with 10-beta recovery media (NEB) then incubated for 1 hour at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... the culture filtrates from methanol-induced cultures were treated with PNGaseF (New England Biolabs), and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α F’Iq cells and transformants were selected on LB agar plates containing carbenicillin (NEB). The DNA sequence of the plasmid inserts for each transformant was verified before and after transformation into STm by electroporation ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with media containing 3 µM SNAP-Cell block (New England BioLabs cat. # S9106S) and cells maintained at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... Media was supplemented with dTTP/dGTP (NEB N0446S) at a final concentration of 200 uM (equimolar ...
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bioRxiv - Biochemistry 2020Quote: ... Diatom exconjugants were selected on 1/2L1 1% agar medium supplemented with 20 μg mL−1 phleomycin (Gold Biolabs) at a diel cycle of 14:10 at ~50 μE ms−1 light intensity.
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bioRxiv - Microbiology 2023Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTT broth was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Colony PCR was performed on Salmonella-positive colonies on blood agar using Taq DNA polymerase (New England Biolabs®) with an annealing temperature of 55°C and an extension time of 2.5 min ...
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bioRxiv - Microbiology 2022Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTwas extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of Universal nuclease (Pierce; 125U/L of culture) and 10 μl of DNaseI (NEB; 20U/L of culture) were added and the mixture allowed to incubate for 10 min at RT ...
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bioRxiv - Genomics 2020Quote: ... Cultures were infected with 8ml M13K07 helper (NEB) for 60min at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... sufficient colonies were scraped from agar plates for >400-fold library coverage and DNA was extracted (T1010L, New England Biolabs). DNA concentrations were measured using a NanoPhotometer N60 (Implen).
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bioRxiv - Microbiology 2022Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTwas extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB)) ...
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bioRxiv - Genetics 2021Quote: ... Plasmid was isolated from the remainder of the original 5-mL culture of the R599A culture using standard methods and digested with PvuI-HF (New England Biolabs #R3151) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... and media were obtained from New England Biolabs (NEB) unless otherwise noted ...
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bioRxiv - Biophysics 2022Quote: ... 100 uL of room temperature SOC media (New England Biolabs) was added to the cells ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with the random colonies selected in LB agar plates with 50 µg/ml carbenicillin using OneTaq® 2X Master Mix (New England Biolabs, USA) and sequenced with Sanger method (Eurofins Scientific ...
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bioRxiv - Microbiology 2021Quote: ... xanthus cultures using the Monarch Total RNA Miniprep Kit (NEB). 109 cells were harvested and resuspended in 200 µl lysis-buffer (100 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled in full media with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and imaged in RPMI without phenol red in a Nikon Eclipse Ti spinning disk confocal microscope with an ORCA-Flash 4.0 camera (Hamamatsu ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then transferred into SOC outgrowth media (950 μL, New England BioLabs) at 37 ◦C for 1 hr before expansion into YT growth media (50 mL ...
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bioRxiv - Genomics 2020Quote: ... Plasmid DNA was purified from liquid cultures using DNA miniprep kit (NEB #T1010).
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bioRxiv - Microbiology 2022Quote: DNA was extracted from single-colony cultures (Monarch Genomic DNA Purification Kit, NEB). Short read libraries were prepared based on the NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... untreated cultures of cortical neurons (DIV8) were lysed in lambda phosphatase buffer (NEB), incubated for 20 min to ensure total lysis ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from the cell culture supernatants and converted to cDNA (NEB). The E gene of stock virus ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Electroporated cells were immediately transferred into pre-warmed SOC recovery media (New England Biolabs) and recovered for 1-2 hours at 37 °C with 250 rpm of shaking ...
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bioRxiv - Genetics 2021Quote: MBP-Stwl antigen was purified from induced bacterial culture using Amylose Resin (NEB: E8021L). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Samples of E.coli cultures were harvested and resuspended in E.coli lysis reagent (NEB, US). Lysates and purified proteins were separated by 4-12% Bis-Tris SDS-PAGE (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The culture supernatants (150 μL) were treated with DNase I (NEB, Ipswich, MA, U.S.A.) to obtain enveloped and encapsidated viral genomes ...
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bioRxiv - Bioengineering 2024Quote: ... media containing 100 μg/mL ampicillin and 109 p.f.u./mL of M13K07 helper phage (NEB) was added for overnight phage amplification in 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... DNA extracted from the 30mL culture was digested with HindIII-HF (#R3104S, New England Biolabs) and BamH1-HF (#R3136S ...
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA were extracted from bacterial cultures using Monarch Plasmid Miniprep Kit (New England Biolabs) and sequenced using the BigDye Terminator v.1.1 kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Turicibacter genes were amplified from template culture lysates with Phusion or Q5 DNA polymerase (NEB) and primers designed to amplify denoted Turicibacter genes ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylated cell lysates or conditioned media were prepared by treatment of PNGase F (New England Biolabs). Samples containing around 30 μg proteins was loaded to SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: ... media containing 100 μg/mL ampicillin and 10^9 p.f.u./mL of M13K07 helper phage (NEB) was added for overnight phage amplification at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... This was added to 5 ml (per 2 L of culture) amylose resin (New England Biolabs) equilibrated in lysis buffer and left on a tube roller shaker at 4 °C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids from O/N cultures were extracted with the Monarch Plasmid Miniprep Kit (New England Biolabs) and sent for sequencing to LGC genomics.
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bioRxiv - Molecular Biology 2022Quote: ... was added to the culture prior to RNA extraction using Monarch total RNA miniprep kit (NEB). Isolated RNA was quantified using Quibit RNA BR assay kit (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... we pelleted each liquid culture by centrifugation and used the Monarch Plasmid Miniprep Kit (NEB #T1010L) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then incubated with amylose affinity resin (NEB, 2mL bead volume/1L bacterial culture) for 1hour at 4°C while nutating ...
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bioRxiv - Genomics 2024Quote: ... The cultures were pelleted and subjected to plasmid DNA isolation using Monarch Plasmid Miniprep Kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Lysates (20 μg) and media immunoprecipitates were diluted to 10 μL in 2X glycoprotein denaturing buffer (NEB), and boiled for 10 min ...