Labshake search
Citations for New England Biolabs :
51 - 100 of 1712 citations for Synthetic Apoptosis Regulator Bcl 2 BCL2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: A synthetic miR-409-5p oligo was 5’ end-labelled with γ-P32 ATP using T4 polynucleotide kinase (New England Biolabs) and purified using G-50 columns ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids were assembled from fragments (linearized vector, PCR products, and/or synthetic DNA) using the NEBuilder Hifi DNA assembly kit (New England Biolabs (NEB) E2621) ...
-
bioRxiv - Systems Biology 2023Quote: ... Cloning of synthetic DNA constructs was conducted using chemically competent DH10-β cells purchased from NEB (New England Biolabs, Ipswitch, MA) or XL-1 Blue (Agilent Technologies ...
-
bioRxiv - Bioengineering 2023Quote: ... was digested with BamHI and then a synthetic double-stranded ultramer (IDT) was incorporated using NEBuilder HiFi DNA Assembly (NEB, E2621) to generate a dCas9-NLS-linker-BamHI-NLS-FLAG expressing plasmid ...
-
bioRxiv - Genomics 2022Quote: ... 500 nL of 5hmC-blocking mix (1 U T4-BGT (NEB, M0357L), 6x UDP-glucose ...
-
bioRxiv - Immunology 2020Quote: ... The peptides were first digested with Endo H (New England Biolabs) to deplete oligomannose- and hybrid-type glycans and leave a single GlcNAc residue at the corresponding site ...
-
bioRxiv - Bioengineering 2020Quote: ... reesei strain by peptide N-glycosidase F (PNGase F, NEB, P0704) (Wang et al ...
-
bioRxiv - Cell Biology 2022Quote: ... or Peptide-N-Glycosidase F (PNGase F) (New England Biolabs, #P0704S) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... and Ph.D.-C7 peptide library (New England Biolabs, Ipswich, MA, USA) for the identification of AbOmpA binding phage ...
-
bioRxiv - Molecular Biology 2020Quote: ... the desired sequences pertaining to the truncated forms of the N protein were subcloned from the synthetic gene using polymerase chain reaction (Phusion polymerase, New England Biolabs, Hertfordshire, UK). All genes were cloned into the modified pet28 vector and gene sequences were confirmed by Sanger Sequencing.
-
bioRxiv - Microbiology 2019Quote: ... Linearized SINV AR86 cDNA plasmid or the described TF mutant derivatives were transcribed into infectious RNA in vitro via SP6 polymerase and a synthetic cap analog (New England Biolabs, Ipswich, MA). RNA was electroporated into baby hamster kidney (BHK ...
-
bioRxiv - Genomics 2019Quote: ... The available CPQ cDNA was previously prepared from total RNA derived from midguts of fifth-instar larvae fed on semi-synthetic diet using a Protoscript II kit (NEB, Ipswich, MA). PCR was carried out on both strains using primers designed to flank the region of the kdr mutation in the para gene (Forward 5’-ACCAAGGTGGAACTTCACAGAT −3’ Reverse 5’-AGCAATTTCAAGAAGTCAGCAACA −3’) ...
-
bioRxiv - Microbiology 2020Quote: ... The Gibson Assembly method was then utilized to assemble the synthetic dsDNA fragments using the Gibson Assembly Master Mix (New England Biolabs, Ipswich, MA) (76) ...
-
bioRxiv - Biochemistry 2021Quote: Peptide-N-glycosidase F (PNGase F) (New England Biolabs Inc., Cat. P0704S) was used for complete removal of N-linked oligosaccharides ...
-
bioRxiv - Plant Biology 2020Quote: ... 1.5 μg of biotinylated histone peptides were incubated with streptavidin beads (NEB) in binding buffer (50 mM Tris-HCl 8.0 ...
-
bioRxiv - Cell Biology 2023Quote: ... separated via self-cleaving T2A peptide was generated via Gibson Assembly (NEB). This cassette including a 5’ chimeric intron and 3’ poly adenylation signal sequence replaces the sequence of Pax7 exon 1 immediately 3’ of the ATG start codon ...
-
bioRxiv - Bioengineering 2019Quote: ... Double stranded DNA was generated by amplification of the synthetic gBlock f1 sequence with Phusion™ polymerase (New England Biolabs, Inc., Ipswitch, MA). The beta-lactamase (bla ...
-
bioRxiv - Genetics 2019Quote: The DNA fragment of the synthetic sgRNA expression cassettes was PCR amplified with high fidelity Taq DNA polymerase (NEB Phusion® Cat#M0530S) using the forward and reverse primers 5’-aggctcccgggtgcgtcgacggtctcaggtcagagcttg-3’ and 5’-gaaagctgggtgattcaagcttggtctcatcagggatccaaaag-3’ respectively ...
-
bioRxiv - Immunology 2022Quote: ... N-glycans were enzymatically removed from the underlying peptides with PNGase F (NEB) overnight at 37 °C ...
-
bioRxiv - Immunology 2019Quote: ... A 7-mer random peptide library (E8100S, Ph.D. -7, New England Biolabs, USA) was panned overnight at 4°C on pooled human IgM adsorbed on polystyrene plates at a concentration of 0.1 mg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... The Peptide-N-Glycosidase F (PNGase F) enzyme (New England Biolabs, catalog #: P0704L)-treated samples served as a control for unglycosylated α1 subunits ...
-
bioRxiv - Immunology 2023Quote: ... N-glycans were enzymatically removed from the tryptic peptides with PNGase F (NEB) overnight at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... 2 μL GlycoBuffer 2 10X (NEB), 2 μL NP-40 ...
-
bioRxiv - Evolutionary Biology 2020Quote: Phage display experiments were performed using the PhD-12 peptide phage display kit (NEB). All steps involving the pipetting of phage-containing samples was done using filter tips (Rainin) ...
-
bioRxiv - Biochemistry 2020Quote: ... tsetse saliva was treated with peptide-N-glycosidase A (PNGase A, New England Biolabs), which releases all N-linked glycans ...
-
bioRxiv - Cell Biology 2019Quote: ... The PfGRP170 transit peptide PCR was digested with Nhe1 and AatII (New England Biolabs) and the GFP PCR was digested with AatII and BglII (New England Biolabs) ...
-
bioRxiv - Neuroscience 2021Quote: ... Deglycosylation was performed with peptide-N-glycosidase F (PNGase F; New England Biolabs, USA) following the manufacturer’s instructions [28] ...
-
bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabsⓇ Inc., MA, USA) was preferred in order to select aggregation inhibitory peptide selection ...
-
bioRxiv - Bioengineering 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs□ Inc., MA, USA) was preferred ...
-
bioRxiv - Bioengineering 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs□ Inc., MA, USA) was used ...
-
bioRxiv - Biochemistry 2020Quote: Tsetse salivary proteins were treated with peptide-N-glycosidase F (PNGase F, New England Biolabs), which cleaves all N-linked glycans except those with an α-1,3 fucose modification of the chitobiose core [20] ...
-
bioRxiv - Neuroscience 2019Quote: ... protein samples were treated with peptide-N-Glycosidase F (PNGase) (New England Biolabs, Whitby, ON) following manufacturers guidelines ...
-
bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
-
bioRxiv - Molecular Biology 2023Quote: ... to prevent exoIII digestion on the arrest peptide side and then with AvrII (NEB, R0174S) to allow for exoIII digestion towards the candidate protein ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... g165 and g166 without the predicted signal peptides were PCR-amplified with Phusion polymerase (NEB) from genomic DNA of P ...
-
bioRxiv - Biochemistry 2023Quote: ... Desalted peptides were dissolved in 1 ml 1 × CutSmart buffer (pH 8, New England BioLabs) and incubated with 50 units of alkaline phosphatase (calf intestinal ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 μL of 2% SDS and 2 μL of proteinase K (New England Biolabs) were added and the solution was incubated at 37°C for 1 hour before purification with the Qiagen DNA clean up kit ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2 μl 10X NEBuffer 2 (New England Biolabs) and nuclease-free water (total of 20 μl ...
-
bioRxiv - Bioengineering 2021Quote: ... 2 μL of 2 U L-1 ExoI (NEB) was added and incubated at 37 °C for 30 minutes then 80 °C for 20 minutes ...
-
bioRxiv - Biochemistry 2020Quote: ... 2 μL of GlycoBuffer 2 (NEB Cat # B0701S, 10X), 2 μL of 10% NP-40 (NEB Cat # B0701S ...
-
bioRxiv - Systems Biology 2024Quote: ... 2 µl of NEBuffer 2 (New England Biolabs B7002) and 1 µl of Klenow large fragment DNA polymerase (New England Biolabs M0210 ...
-
bioRxiv - Biochemistry 2019Quote: ... we used a commercial assay kit (Ph.D.-7 Phage Display Peptide Library Kit, New England Biolabs) and followed the recommended protocol for “solution phase panning with affinity bead capture” with the following modifications ...
-
bioRxiv - Synthetic Biology 2021Quote: ... DNA templates for MS-QBiC peptide synthesis were amplified by PCR using Taq DNA polymerase (NEB) with a T7 promoter primer (5′-GGGCCTAATACGACTCACTATAG-3′ ...
-
bioRxiv - Biochemistry 2020Quote: ... Desalted peptides were dissolved at a concentration of 3 µg/µL in 1x CutSmart buffer (NEB, 50 mM potassium acetate ...
-
bioRxiv - Cell Biology 2022Quote: ... enzyme digestion or Peptide-N-Glycosidase F (PNGase F) (New England Biolabs, Ipswich, MA, catalog #: P0704L) enzyme digestion was performed according to manufacturer’s instruction and the published procedure [49].
-
Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: Lysine-succinylated peptides were enriched using agarose-conjugated pan anti-succinyllysine antibody (PTM Biolabs, Hangzhou, China). In brief ...
-
bioRxiv - Genetics 2023Quote: ... following which they were incubated in blocking buffer composed of 0.5 mg/ml BSA (New England Biolabs, #B9001S), 80 units of RNAse-OUT RNAse inhibitor (Invitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... pUHES:UmPit2+SP:mCherry with its natural signal peptide was generated via NEBuilder HiFi DNA Assembly Cloning Kit (NEB) by combining PCR-amplified UmPit2+SP:mCherry using primers 2127 + 2128 and PCR-amplified pUHESdest using primers 2125 + 2126 without UhAvr1 signal peptide ...
-
bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...