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Citations for New England Biolabs :
1 - 50 of 1712 citations for Synthetic Apoptosis Regulator Bcl 2 BCL2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 2 μL of peptide N-glycosidase F (New England Biolabs) were added ...
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bioRxiv - Genomics 2020Quote: Synthetic SARS-CoV-2 RNA templates were serially diluted and amplified by RT-LAMP using the WarmStart LAMP Kit (NEB). LAMP primers were added to a final concentration of 0.2µM for F3 and B3 ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged and resuspended in 600 μl Blocking buffer (PBS + 2 % BSA (B9000S, New England Biolabs)) and incubated for 5 min on ice ...
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bioRxiv - Biochemistry 2019Quote: ... The synthetic gene fragments were amplified by Phusion DNA polymerase (NEB) with the primer pair 10Hfor and 10Hrev (Supplementary File 4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Each synthetic DNA fragment was digested by SfiI (New England Biolabs) and ligated into SfiI digested backbone from pCH117 using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: Protein G magnetic beads were washed 2-3x with 1ml blocking buffer (20 mM Hepes, 150 mM KCl, 20% Glycerol, 0.5 % Tween, BSA (Biolabs), Heparin 0.2 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were heated for 1 minute at 100°C followed by the addition of 2 μL of peptide N-glycosidase F (New England Biolabs) to release the N-glycans ...
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bioRxiv - Molecular Biology 2023Quote: ... Three peptide phage libraries from NEB “New England Biolabs” ...
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bioRxiv - Genomics 2023Quote: ... Blocking was achieved using 1% BSA (NEB) and 0.1% Tween-20 in 1× PBS for 1 h at room temperature ...
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Phospho-sRNA-seq reveals extracellular mRNA/lncRNA fragments as potential biomarkers in human plasmabioRxiv - Molecular Biology 2019Quote: ... synthetic and plasma RNA samples were pretreated with T4 polynucleotide kinase (NEB, Ipswich, MA, US) using an RNA input of 7 ul in a final reaction volume of 10 ul and following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Synthetic oligonucleotides were labeled with [γ 32P]-ATP and T4 polynucleotide kinase (New England Biolabs) at their 5’ end ...
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bioRxiv - Biochemistry 2021Quote: ... the synthetic O-glycopeptide was incubated with 200 U of α2-3,6,8 Neuraminidase (New England Biolabs) for 16 h at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... These synthetic fragments were cloned into ccdB compatible sites in pDD282 by Gibson assembly (New England Biolabs). Homology arms for each targeting construct are about 1kb on 5’ and 3’ to the epitope insertion site (see Tables S3 for additional details) ...
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bioRxiv - Biochemistry 2022Quote: ... 50 pmol of in vitro transcribed or synthetic RNA was incubated with 10 units T4 PNK (NEB) and 2 μl γ-32P-ATP in 1X PNK buffer and incubated for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... A synthetic DNA block (IDT) coding for the scrambled E domain was subcloned using Gibson-cloning (NEB). GST-α-syn 96-140 Scr and GST-α-syn 96-110 Scr plasmids were synthesized by GenScript (Piscataway ...
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bioRxiv - Molecular Biology 2023Quote: ... using gBlocks synthetic DNA fragments (Integrated DNA Technologies) and NEBuilder HiFi DNA assembly mix (New England Biolabs). HEK293 cells in 12-well plates were transfected with 1.6 μg of CircRNA Mini or mc2 plasmid encoding circMLLT2(3,4 ...
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bioRxiv - Microbiology 2022Quote: ... Peptide-N-Glycosidase F (PNGase F; NEB, P0704) treatment of lysates was performed as described in the manufacturer’s directions ...
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bioRxiv - Genomics 2021Quote: Genomic DNA and synthetic oligonucleotides were digested to nucleosides by treatment with the Nucleoside Digestion Mix (NEB #M0649S) at 37 °C for 3 h ...
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bioRxiv - Microbiology 2022Quote: ... resulting in a linearized vector into which synthetic variant spike genes can be assembled using Gibson Assembly (NEB). The Gibson Assembly reaction was then transformed into TransforMax™ EPI300™ Electrocompetent E ...
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bioRxiv - Genetics 2022Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3uM blocking oligo ...
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bioRxiv - Cancer Biology 2022Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3 µM blocking oligo ...
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bioRxiv - Molecular Biology 2021Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3uM blocking oligo ...
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bioRxiv - Microbiology 2021Quote: ... Peptide-N-glycosidase F (PNGase F) (New England BioLabs) was used to remove all N-linked oligosaccharides for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... or for peptide N-glycosidase F (PNGase-F, NEB) digestion using 500 units PNGase-F for 1,5h at 37°C ...
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bioRxiv - Genetics 2021Quote: ... The synthetic oligo was cloned into the plasmid backbone by using the Gibson assembly kit (NEB, Gibson Assembly®) and the ligation reaction was carried out for 1 hour at 50 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... to generate the synthetic enhancer eGFP plasmid reporter (pTol2-synthetic enhancer-E1b-eGFP-Tol2) using HiFi DNA Assembly following manufacturer’s instructions (New England Biolabs). Reporter plasmid sequences were verified by Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... were prepared by cloning the codon-optimized (E.coli expression) synthetic gene fragments into pMAL-p2x vector (New England Biolabs) for periplasmic expression in BL21-CodonPlus(DE3)-RIPL (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... Each of them was made by assembling a synthetic gBlock DNA fragment with a PCR-amplified Kanamycin resistant backbone using NEBuilder DNA Assembly (New England Biolabs). The region to be PCR-amplified in each vector contains a gRNA scaffold followed by either a tRNA or the U6:3 promoter ...
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bioRxiv - Biochemistry 2020Quote: ... The homologous sequences between the synthetic DNA and the digested vector allowed them to be joined by Infusion reaction (NEBuilder, New England Biolabs).
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bioRxiv - Plant Biology 2021Quote: Blunt ended Myc tagged GUX and AtMAGT1 CDSs were amplified from a synthetic construct (synthesised by Genewiz) with Q5 DNA Polymerase (NEB) using primers detailed in Table S1 ...
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bioRxiv - Biochemistry 2022Quote: Purified in vitro transcripts or synthetic RNA oligonucleotides were capped using Vaccinia RNA capping enzyme (Vaccinia Capping System, New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the SYLF domain was mutagenized by ordering a synthetic DNA sequence carrying point mutations (Table 4) and replaced by restriction enzyme digestion with NcoI (NEB) and XbaI (NEB) ...
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bioRxiv - Microbiology 2020Quote: The SARS2-CoV-2 vaccine candidates were cloned by combining the S cDNA (obtained after PCR on overlapping synthetic cDNA fragments; IDT) by a NEB Builder Cloning kit (New England Biolabs) into the pShuttle-YF17D backbone ...
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bioRxiv - Molecular Biology 2020Quote: ... and inserting a synthetic gBlock (IDT) containing an FRT5 site followed by an in-frame stop codon via NEB HiFi Assembly (NEB) to create pTaDaG2-FRT5 ...
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bioRxiv - Biochemistry 2020Quote: ... was produced by cloning five synthetic gene fragments in pE-SUMO (Life Sensors, PA) using NEBuilder HiFi DNA Assembly (New England Biolabs). For purification ...
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bioRxiv - Biochemistry 2021Quote: ... vector were constructed by digesting pMS329 and pWZ414-F12 respectively with ClaI/AgeI and with BlpI/BamHI to remove a segment of their H3 genes followed by recombination with synthetic DNA fragments (Twist Bioscience) containing the R49C mutation by the Gibson assembly protocol (New England Biolabs). Similarly ...
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bioRxiv - Microbiology 2021Quote: ... pSEVA_331 and the synthetic constructs were amplified using primers in Supplementary Table S3 and Q5 DNA Polymerase (New England Biolabs), and cloned by restriction digest using the enzymes indicated in Supplementary Tables S3 and S4 ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were assembled from fragments (linearized vector, PCR products, and/or synthetic DNA) using the NEBuilder Hifi DNA assembly kit (New England Biolabs (NEB) E2621) ...
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bioRxiv - Microbiology 2022Quote: ... These shRNA cassettes were assembled in the 3’UTR of a synthetic eGFP gene (Twist Bioscience) via two-fragment Gibson assembly into the pSTZ backbone linearized by EcoRI and BamHI (NEB).
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bioRxiv - Molecular Biology 2023Quote: Individual sgRNA expression vectors were cloned by first annealing complementary synthetic oligonucleotide sequences containing each sgRNA protospacer (Table S3) flanked by BstXI (New England Biolabs) and BlpI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... at the HHF2 location by digestion with BamHI and NcoI followed by recombination with a synthetic 2xV5-HHF2 fragment using the Gibson Assembly protocol (New England Biolabs).
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bioRxiv - Genomics 2024Quote: The Zhang Lab General Cloning Protocol was used to insert annealed synthetic-oligonucleotide gRNA into the BbsI (New Englands Biolabs) restriction site to clone single gRNAs into PX458 and EDCas9 ...
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bioRxiv - Molecular Biology 2024Quote: ... Synthetic genomes were in vitro assembled using PCR-generated fragments and Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, BioLabs) with template DNA consisting of φ2638A WT gDNA that had been circularized through annealing of the terminal cos sites by heating gDNA to 65°C for 10 mins ...
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bioRxiv - Bioengineering 2019Quote: ... 7-mer random peptide phage library (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Bioengineering 2019Quote: ... 7-mer random peptide phage library (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2022Quote: ... and tryptic peptides were generated using Trypsin-Ultra (P8101S, NEB) following the protocol described previously2 ...
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bioRxiv - Neuroscience 2022Quote: ... or peptide N-glycanase (PNGase, New England Biolabs, 1,000 units) for 6 h ...
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bioRxiv - Microbiology 2021Quote: ... To construct a plasmid containing the full length genome we obtained seven synthetic fragments with overlapping sequences and assembled them via Gibson Assembly (NEBuilder® HiFi DNA assembly, NEB). A subclone containing the reminder of the viral sequences and restriction sites required to clone in the fragments was generated in a modified pBluescript plasmid41 ...
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bioRxiv - Molecular Biology 2020Quote: Synthetic RNA fragments bearing a 3′P were subjected to 5′ phosphorylation with T4 PNK 3′ minus (NEB, cat n° M0236S), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Reporter virus RNAs were transcribed from XhoI-linearized plasmid templates pYF17D/Nluc and pYFΔSK/Nluc by using SP6 RNA polymerase in the presence of the ARCA synthetic cap analog (New England Biolabs, Ipswich, MA). Primary stocks of reporter virus were generated by electroporation of BHK-21 clone 15 or BHK-NS1 cells with YFV RNA transcripts ...