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Citations for New England Biolabs :
51 - 100 of 1950 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: Purified RNA (5 ug) was reverse transcribed using Random Primer 9 (NEB) and SuperScript II reverse transcriptase under error prone conditions as described Smola et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ug of DNA was incubated with 6U RNAse H (NEB, M0297) at 37 °C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ug of RNA was reverse transcribed using the Lunascript Supermix (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... 10 μg of Δorf library plasmid was digested with 100 units of AsiSI for vector A or PmeI for vector P (NEB, R0630 or R0560) at 37°C overnight ...
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bioRxiv - Genomics 2024Quote: ... cells were washed three times with PBS-T and incubated with a gap-fill Phusion mix (1x Ampligase Buffer, 0.4 U/mL RNase H, 100 nM padlock probe oPD_CROPseq_iT7, 0.0125 U/mL NEB Phusion polymerase ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were preserved in a 1:3 glacial acetic acid: methanol (Biolabs-chemicals) solution and karyotyped using g-banding.
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bioRxiv - Cell Biology 2021Quote: 500mM p-Nitrophenyl Phosphate (pNPP, NEB, P0757S) substrate solution was diluted to 20 mM in phosphatase reaction buffer (PRB ...
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bioRxiv - Bioengineering 2024Quote: ... M13mp18 circular DNA (P- 107, Bayou Biolabs) was used as the scaffold for the staples (IDT) ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with 100 μg/ml proteinase K (NEB P8107) for 16 h at 50 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 U/mL DNA Polymerase I Large (Klenow) Fragment (NEB, M0210) and 300 U/mL T4 DNA ligase (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... were pooled and loaded onto 1 ml 100% amylose resin (NEB). The resin was washed with 10 column volumes of wash buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA Polymerase I Large (Klenow) Fragment 100 U/ml (NEB M0210L) and T4 DNA ligase 300 U/ml (Thermo Scientific EL0013) ...
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of 1 unit mL-1 SfoI restriction enzyme (NEB) was injected to generate blunt-end DNA molecules.
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids (1 ug) were linearized using BamHI restriction enzyme (NEB, Ipswich, MA, USA), column-purified using the DNA Clean & Concentrator −5 Kit (Zymo Research Corporation ...
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bioRxiv - Genomics 2020Quote: DpnII digest:20 ug genomic DNA + 20 uL DpnII buffer (New England Biolabs) + 4 uL DpnII 9New England Biolabs ...
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bioRxiv - Genomics 2020Quote: DpnI digest: 10 ug genomic DNA + 10 uL CutSmart Buffer (New England Biolabs) + 2 uL DpnI (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... the culture filtrates from methanol-induced cultures were treated with PNGaseF (New England Biolabs), and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis ...
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bioRxiv - Immunology 2019Quote: ... containing 2 µl of 2X lysis buffer (100 mM Tris.HCl, pH 8.0, 100 mM NaCl, 40 µg/ml Proteinase K (New England BioLabs, P8107S), 0.4% SDS ...
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bioRxiv - Biochemistry 2023Quote: ... In order to remove the EndoHf from the HPAMPD the supernatant was brought to 100 mM Tris -HCl pH 7.5 and 100 mM NaCl and applied to a one ml amylose resin (NEB) column at a rate of about 1 drop per second at 25° C ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from total RNA using the rflP specific primer (P-LCM621) and template-switching oligonucleotide (P-LCM619) using RT Template Switching Enzyme Mix (New England Biolabs). The 5’-end end of the rflP cDNA was enriched by PCR using the primers P-LCM620 and P-LCM622 ...
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bioRxiv - Neuroscience 2022Quote: ... p-GSK-3β (S9) (NEB D85E12, 1:50,000), GSK-3β (NEB 27C10 ...
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bioRxiv - Cancer Biology 2019Quote: ... and then resuspended in sorting buffer (PBS with 0.5% BSA, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 µg/mL DAPI and 100 U/mL DNaseI, NEB) and incubated for 30 minutes at room temperature before sorting ...
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bioRxiv - Neuroscience 2021Quote: Libraries were prepared from 1 ug RNA using a library prep kit (NEB #7770), rRNA depletion kit (NEB #E6310) ...
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bioRxiv - Microbiology 2021Quote: ... acnes lysates were incubated with 0.5 ug plasmid DNA together with different buffers (NEB) supplemented with or without ATP or SAM ...
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bioRxiv - Genomics 2021Quote: ... 12 μL of 10 mg/mL Bovine Serum Albumin (100× BSA, NEB), 5 μL of 400 U/μL T4 DNA Ligase (NEB M0202) ...
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bioRxiv - Genomics 2023Quote: ... 12 μL of 10 mg/mL Bovine Serum Albumin (100 × BSA, NEB), 5 μL of 400 U/μL T4 DNA Ligase (NEB #M0202) ...
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bioRxiv - Immunology 2022Quote: ... 2018” tagmentation mix were either sorted into plates containing RCB buffer for condition “hiSDSprotK-TWEEN” (2 x RCB: 100 mM Tris-HCl pH 8, 100 mM NaCl, 40 µg/mL Proteinase K (NEB), 0.4 % SDS (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and de-N-glycosylated using 500 U N-glycosidase F (PNGase F, New England Biolabs) for 12 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... except after the Ni-NTA step it was concentrated (1 mg/ml in 0.2 ml) and shaved with endoglycosidase D (10 μl, 100 unit) (New England BioLabs) for 16 hrs at 4°C prior to gel filtration ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ug from each cell line was digested with 25 units EcoRI (New England Biolabs) in 100 ul total volume ...
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bioRxiv - Biochemistry 2022Quote: ... 2 ug of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA was digested with 20 U of RNase H (NEB #M0297S) at 37°C for 6 hours to serve as a negative control ...
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bioRxiv - Neuroscience 2023Quote: ... and p{ywing2+} were combined by HiFi assembly (NEB). The product was transformed into DH5α Chemically Competent Cells (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg/mL cycloheximide) supplemented with 0.1 U/µL murine RNase Inhibitor (NEB), and passed through a G25 needle 25 times ...
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bioRxiv - Bioengineering 2022Quote: ... or 1 μg Asp-N (NEB, England) for overnight digestion respectively ...
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bioRxiv - Immunology 2020Quote: ... deglycosylated with N-glycanase (New England Biolabs), and digested overnight with LysC protease (ThermoFisher scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... or N-glycosilase F (PNGaseF, NEB P0704S), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... endoproteinase Asp-N (New England Biolabs, #P8104S), Glu-C (Sigma ...
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bioRxiv - Genomics 2022Quote: ... 3 ug of input DNA was dephosphorylated with Quick CIP (New England Biolabs, cat no M0508). Following enzyme inactivation with alkaline phosphatase ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were further quenched by the addition of 0.2 ug of RNAse A (New England Biolabs) and incubation at 37 °C for 20 min ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.8) and 100 μg/ml of proteinase K (New England Biolabs, Ipswich, MA) for 15 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... proximity ligated sample was treated with 100 μL Proteinase K (NEB P8107S-800U/ml), 100μL 10% SDS (Invitrogen Cat no ...
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bioRxiv - Neuroscience 2020Quote: TEV protease (TEV-P) was purchased from NEB (Cat#P8112S). PFFs were prepared as above and premixed 1:10 (for a final concentration of 15 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’P was created by T4 PNK treatment (NEB, M0201S), done according to the NEB T4 PNK protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsin digest of PDI was followed by digestion of N-glycans with endo-β-N-acetylglucosaminidase H (500 U; NEB) in sodium citrate buffer (50 mm ...
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bioRxiv - Microbiology 2022Quote: ... Peptide-N-Glycosidase F (PNGase F; NEB, P0704) treatment of lysates was performed as described in the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2023Quote: ... coli BL21 lysY/Iq (NEB cat n° C3013). The expression plasmid was transformed via heat-shock followed by selection of clones on LB-Agar plates supplemented with 100 µg/mL carbenicillin ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were treated with RNAse (100 μg/ml) at room temperature for 15 min and then with proteinase K (825 μg/ml, NEB™, P8107S) for 1 hour at 65°C ...