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Citations for New England Biolabs :
401 - 450 of 1950 citations for P N Nonylphenol Diethoxylate Ring 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Mutations to the specificity of guides in pTREX-n-Cas9-noTags was performed using a Q5 mutagenesis kit (NEB, Ipswich, Massachusetts). Guides targeting the mitochondrial glutamate dehydrogenase (mGDH ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylation was performed using Peptide-N-Glycosidase F (PNGase F) at 37 °C for one hour according to the supplier’s protocol (New England Biolabs, Hitchin, UK). Enzyme purity and molecular weight were estimated by 12 % SDS-PAGE using mini-PROTEAN 3 system (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... An IFT144 construct lacking the N-terminal β-propeller domain (residues 2–349 inclusive; IFT144ΔNFLAG) was made using the Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... pH 8.0 and adding 2ul/5-10mg dry weight (DW) of PNGaseF (Peptide-N-Glycosidase F) (New England Biolabs, Cat. #P0704S). Samples were incubated with continuous agitation at 150 rpm (Stuart Horizontal Shaker ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylation was performed using Peptide-N-Glycosidase F (PNGase F) at 37 °C for one hour according to the supplier’s protocol (New England Biolabs, Hitchin, UK). Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... a 3′ RNA adapter with a phosphate on its 5’ (5’- /5Phos/r(N:25252525)r(N:25252525)r(N:25252525)rUrGrGrArArUrUrCrUrCrGrGrGrUrGrCrC rArArGrG/3SpC3/-3’) end was ligated to RNA 3’ ends using T4 RNA ligase 1 (NEB, M0437) at 25 °C for 75 min (with gentle agitation every 10 min) ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...
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bioRxiv - Biophysics 2024Quote: ... First the 5’ triphosphate of RNA was converted into a 5’ monophosphate by incubating 100 µg RNA with 100 units of RNA 5’ Pyrophosphohydrolase (NEB, Ipswich MA) at 37°C for 1 hour within a 100 µl reaction volume ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of cells was incubated with 1 μl M13KO7 (1011 plaque-forming units per ml, pfu/ml) (NEB) for 10 min on ice to allow attachment ...
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bioRxiv - Synthetic Biology 2021Quote: ... and/or 100 U SP6 RNA Polymerase (NEB #M0207L), 15μM of DFHBI-1T and/or Malachite Green dye ...
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bioRxiv - Microbiology 2020Quote: ... DNA was digested with 100 units of MboI (NEB) and ligated in the nucleus followed by in situ Hi-C protocol [42] ...
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bioRxiv - Pathology 2020Quote: ... gDNA (100 ng) was digested with TaqaI (NEB R0149) at 65⁰C for 2h followed by MspI (NEB R0106 ...
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bioRxiv - Microbiology 2021Quote: ... or 100 nM TMR substrate for SNAPtag (S9105S, NEB), for 30 min anaerobically ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was digested with 100 units of MboI (NEB) at 37°C for 2 hours and subsequently incubated at 62°C for 20 minutes to inactivate the MboI ...
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bioRxiv - Cancer Biology 2021Quote: ... chitin magnetic beads (100 μL, E8036S, New England Biolabs) were added to the reaction mixture.
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bioRxiv - Cancer Biology 2022Quote: ... 100 U exonuclease III (both from New England Biolabs), and incubation for 60 min at 37°C and 20 min at 80°C.
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bioRxiv - Neuroscience 2022Quote: ... 100 ul of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 ul of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 uL T4 DNA Ligase (New England Biolabs; M0202L), 100 uL BSA 10 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... microcapsules were treated with 100 U Exonuclease I (NEB) for 15 minutes at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... 100 ul of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 ul of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 ul of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 ul of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 U of M-MLV Reverse Transcriptase (NEB; M0253S), M-MLV RT buffer (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μM RSH 5194 and 1x UDG buffer (NEB)48 ...
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bioRxiv - Bioengineering 2023Quote: 100 μM EnGen® Lba Cas12a (New England Biolabs) was diluted as needed in the supplied Cas12a diluent ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 100 μl DpnII buffer (New England Biolabs), and pelleted again ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of TEV protease (New England Biolabs, #P8112S) was added ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was digested overnight with 100 units MboI (NEB) restriction enzyme then the ends filled using dTTP ...
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bioRxiv - Genomics 2023Quote: ... plus 100 U of the MboI enzyme (NEB #R0147), then incubating for ≥2 hours at 37 °C in a Thermomixer at 900 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... or RNase If (1:100 diluted, New England Biolabs) in buffer 3 (100 mM NaCl ...
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bioRxiv - Genomics 2023Quote: ... 100 μL of RNAse Inhibitor (New England BioLabs M0314L) was added to 250 μL of Imaging Buffer Activator (PN 203000015 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM DTT) with 100 units of MNase (NEB), incubated at 37℃ for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 47 ml water) and 4 µL 10mg/ml Proteinase K (P8108S, NEB). The samples were incubated for 8 hours at 55 °C followed by 10 min at 98 °C to inactivate the Proteinase K ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 ml ScaI (BioLabs), followed by 3 h of incubation with a fresh portion ...
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bioRxiv - Molecular Biology 2020Quote: ... BSA (2mg/ml, NEB), dextran sulfate (10% ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1mg/mL BSA (NEB), 0.1 mg/mL Baker’s Yeast tRNA (Sigma R5636) ...
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bioRxiv - Microbiology 2023Quote: ... 20μl/ml DNase (NEB) and RNase cocktail (Amersham) ...
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bioRxiv - Bioengineering 2024Quote: ... – 400 U/mL (NEB); Jack bean hexosaminidase (JBH ...
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bioRxiv - Biochemistry 2020Quote: ... and peptides in one aliquot were deglycosylated by further treatment with 125 U of Peptide-N-Glycosidase F (PNGase F) (New England BioLabs, MA, USA) for 16 h at 37 °C.
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bioRxiv - Genomics 2020Quote: ... sticky ends were filled by incubating the nuclei for 4h at RT with DNA Polymerase I (New England Biolabs, Cat. N: M0210) and a biotin-14-dATP (Life Technologies ...
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bioRxiv - Genomics 2019Quote: ... were used to PCR amplify both wild type and RRM3 Mt cDNA inserts for ligation into a pET28b vector containing an N-terminal 10xHis-SUMO tag using the Quick Ligation kit (NEB, Ipswich, MA). Plasmids containing the cDNA of interest were verified by Sanger sequencing prior to expression (Quintara Biosciences ...
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bioRxiv - Systems Biology 2019Quote: ... The cDNA inserts were PCR-amplified using primers specific for either the N- or C-terminal libraries using Phusion DNA polymerase (New England Biolabs, Ipswich, MA). Amplicons were PCR-purified using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA fragment encoding Sd223-440 was PCR amplified and inserted into a pET28-derived vector providing an N-terminal His-HRV3C-Avi-tag by HiFi DNA Assembly Cloning (New England Biolabs, Ipswich, MA) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... was PCR amplified and inserted into a pET28-derived vector providing an N-terminal His-HRV3C-tag by HiFi DNA Assembly Cloning (New England Biolabs, Ipswich, MA) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... the desired sequences pertaining to the truncated forms of the N protein were subcloned from the synthetic gene using polymerase chain reaction (Phusion polymerase, New England Biolabs, Hertfordshire, UK). All genes were cloned into the modified pet28 vector and gene sequences were confirmed by Sanger Sequencing.
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bioRxiv - Microbiology 2020Quote: ... 25 μg of protein was precipitated from cell lysate as described in Methods and treated with peptide-N-glycosidase F (PNGase F; New England Biolabs, MT, USA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Genomic (g)DNA was extracted from muscle biopsies (from n=27 participants) and isolated using the Monarch kit for DNA isolation (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: The 16S rRNA V4 region was amplified using the following primers 515f-Y: GTGYCAGCMGCCGCGGTA and 806r-N: GGACTACNVGGGTWTCTAAT and The Q5® high-fidelity DNA polymerase kit (New England BioLabs, UK). 2μl of extracted DNA was added to a PCR reaction mix prepared by mixing a final concentration of 1X Q5 reaction buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... µM GAT-7N primers (5′- GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L) in the programmable thermal cycler for 11 cycles ...
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bioRxiv - Neuroscience 2021Quote: ... with 1% (w/v) octylglucoside containing 5 U endoglycosidase H (Endo H) or peptide: N-glycosidase F (PNGase F; both NEB, Frankfurt, Germany) at 37°C for 1 h ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of TET21-N cells (Control and 24 h doxycycline) was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, Hitchin, UK), from which complementary DNA (cDNA ...