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Citations for New England Biolabs :
51 - 100 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... was used to cut out Dlx enhancer and HBB promoter with MluI and EheI (NEB: R0606S).
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bioRxiv - Synthetic Biology 2021Quote: PCR amplicons corresponding to the enhancer segments were generated using Q5 polymerase (New England Biolabs M0492S), except for segment 2 which was generated with Kapa polymerase supplemented with 1 M Betaine ...
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bioRxiv - Genomics 2023Quote: ... Twist BioScience) and then assembled into pNL3.3 enhancer reporter plasmids using Gibson Assembly (New England Biolabs). Each cloned reporter plasmid was verified by Sanger sequencing (Quintara Bio) ...
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bioRxiv - Plant Biology 2019Quote: ... 2 µg total RNA was incubated at 37°C for 30 min together with DNaseI (2 U, NEB). To inactivate the DNaseI ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37 °C for 2 hours followed by digestion with PspXI (NEB) in rCutsmart™ Buffer (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... The lenti-sgRNA puro vector was digested with EcoRI for 2h at 37°C followed by digestion with BsmBI for 2 hours at 55°C and treatment with 4 μl of rSAP (NEB) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cosmids were then digested for 2 hours at 37°C and heat-inactivated for 20 minutes at 80°C using SpeI-HF (New England Biolabs). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Genomics 2021Quote: Putative enhancers were amplified from genomic DNA with Phusion High-Fidelity DNA Polymerase (New England Biolabs, # M0530). Betaine (1 M final concentration ...
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bioRxiv - Genomics 2021Quote: ... the CMV enhancer/promoter region was amplified from the pcDNA3.0 backbone using Phusion High-Fidelity Polymerase (NEB) and subcloned into the pUC19 vector upstream of the circEfnb2 or circMini backsplicing cassettes ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µl was used for BglII digestion (NEB, 2 hours at 37°C) and the products were loaded on a 2% agarose gel to confirm the pAS insertion ...
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bioRxiv - Cell Biology 2021Quote: ... and lysate was incubated 37°C 60 min in 2 units CIP (NEB) per 50 μL reaction containing 50μg of total protein ...
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bioRxiv - Immunology 2020Quote: ... GC-enhancer buffer and 0.25 μl of Q5® Hot Start High-Fidelity DNA Polymerase (NEB, Massachusetts, USA). Amplification was done after an initial phase of denaturation (95 °C ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed four times with 2×SSC and stored at 4 ⁰C in 2×SSC supplemented with 1:1000 murine RNase inhibitor (NEB M0314L) prior to imaging.
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bioRxiv - Developmental Biology 2021Quote: ... and 2 hours at 55°C with 0.1 mg/ml Proteinase K (NEB P8107S).
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bioRxiv - Plant Biology 2021Quote: ... for 3 hours at 55°C and M-2 was digested with XhoI (NEB) overnight at 37°C ...
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bioRxiv - Genetics 2022Quote: Candidate enhancers were amplified from human genomic DNA using Q5 Hot-Start High-Fidelity DNA polymerase (New England Biolabs) and directionally cloned into the pGL3-Promoter plasmid (pGL3-P ...
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bioRxiv - Genomics 2020Quote: ... 0.5 μL of Ultra II Ligation Module Enhancer and 10 μL of Ultra II Ligation Module master mix (NEB) made up of a total of 20 μL with NFW ...
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bioRxiv - Genomics 2019Quote: Enhancer candidate regions were cloned into an Hsp68-promoter-LacZ reporter vector39 using Gibson cloning47 (New England Biolabs [NEB]). Transgenic mouse embryos were generated by pronuclear injection ...
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bioRxiv - Genomics 2019Quote: Enhancer candidate regions were cloned into an Hsp68-promoter-LacZ reporter vector39 using Gibson cloning47 (New England Biolabs [NEB]). Transgenic mouse embryos were generated by pronuclear injection ...
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bioRxiv - Neuroscience 2020Quote: We selected 17 sSNVs identified in brain-active enhancers and attempted cloning and site-directed mutagenesis (New England Biolabs) to recreate the mutations ...
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bioRxiv - Synthetic Biology 2023Quote: ... to generate the synthetic enhancer eGFP plasmid reporter (pTol2-synthetic enhancer-E1b-eGFP-Tol2) using HiFi DNA Assembly following manufacturer’s instructions (New England Biolabs). Reporter plasmid sequences were verified by Sanger sequencing ...
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bioRxiv - Developmental Biology 2019Quote: Pacy-2::acy-2 genomic was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg RNA were incubated for 30 minutes at 37°C with 2 μl RNA 5’ Pyrophosphohydrolase (NEB, M0356, 5,000 units/ml). The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated at 37 °C for 2 hours in 1x T4 DNA ligase buffer (NEB). Following this incubation 1200 units T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... Mif2 was treated for 2 h at 30 °C with lambda-phosphatase (New England Biolabs) according to the manufacturer’s instruction ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Samples were cooled to 42 °C and 2 μl of β-agarase I enzyme (NEB) was added and incubated at 42 °C for 90 min with occasional vortexing ...
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bioRxiv - Genetics 2021Quote: ... We then PCR amplified enhancers and promoters separately from the same array using Q5 high-fidelity DNA polymerase (NEB M0492). We amplified enhancers in four 50uL PCR reactions (98°C for 30 seconds ...
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bioRxiv - Molecular Biology 2020Quote: ... the SV40 promoter was removed from the luciferase-enhancer plasmids by restriction digestion with BglII and HindIII-HF (both NEB). The Fgf5 promoter region - encompassing the 300 bp region containing most of transcription initiation events in PRO-Cap-Seq data at the 5’ UTR of the gene plus 100 bp of flanking nucleotides on each side - was amplified by PCR and inserted in place of the SV40 promoter by Gibson Assembly ...
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bioRxiv - Plant Biology 2023Quote: ... and enhancer fragment combinations were cloned upstream of the 35S minimal promoter by Golden Gate cloning using BsaI-HFv2 (NEB). The resulting libraries were bottlenecked to yield 10–20 barcodes per enhancer.
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bioRxiv - Bioengineering 2024Quote: ... Linkers were then ligated to the DNA using the NEB Next Ultra II Ligation Master Mix and Ligation Enhancer (New England Biolabs). The CAST-seq PCR reactions were performed in forward and in reverse in respect to the cleavage site ...
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bioRxiv - Genetics 2024Quote: QF2-T2A-LexA-GAD ORF PCR-amplified with FRT-QF2-fwd + FRT-lexA-GAD-rev primer pair – used Hot-Start Q5 polymerase with GC enhancer (NEB);
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bioRxiv - Microbiology 2021Quote: ... cDNA synthesis was performed for 60 min at 42 °C and 5 min at 80 °C using ProtoScript II First Strand cDNA synthesis kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 30 seconds at 65°C and 30 seconds at 72°C using the NEBNext® Multiplex Oligos kit (NEB, UK). The expected library size was ∼200-250bp ...
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bioRxiv - Molecular Biology 2023Quote: ... Second-strand DNA synthesis was done by ligating 10 µM of a blunt-end duplex comprised of a 32-bp Illumina R1-3’SpC3 and 5’-phosphorylated R1R-3’SpC3 DNA (pre-annealed by incubating at 95°C for 3 min and slowly cooling to 25°C) using a Quick ligase kit (New England Biolabs) according to manufacturer’s protocol followed by clean-up as described above ...
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bioRxiv - Genetics 2021Quote: ... We then recombined 500ng of this digestion (including ∼4.4kb of backbone vector and 250bp of filler sequence including a spliced region and truncated GFP ORF) with 150ng of both the purified enhancer and promoter products using Gibson assembly (NEB, E2611) for 1 hour at 50°C in a 40uL reaction and purified the reaction using 1X volume AMPure XP with 3 total ethanol washes.
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bioRxiv - Synthetic Biology 2023Quote: ... All Streptomyces colony PCRs were performed using Q5® High-Fidelity DNA Polymerase with GC Enhancer (New England BioLabs Inc., U.S.A).
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bioRxiv - Microbiology 2023Quote: ... The fragments containing Himar transposons were enriched with a two-step nested PCR strategy using Q5 DNA polymerase with GC enhancer (New England Biolabs, M0491) using primers (Table S7 ...
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bioRxiv - Genomics 2023Quote: ... Each tested enhancer sequence was cloned into the corresponding dual-enSERT-1 vector using NotI digestion followed by Gibson assembly methods (NEB, E2611) (Gibson et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... for 2 hours at 37°C in 1X T4 PNK buffer (New England Biolabs, cat#B0201S). Radioactively labeled tRNAs were produced by T7 in vitro transcription in the presence of [α-32P]-UTP (PerkinElmer ...
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bioRxiv - Genomics 2022Quote: ... cells were digested for 2 hours at 37°C using 375U of MboI (NEB, Cat. #R0147). After biotin filling ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng of material was fragmented for 8 min at 37 °C and A-tailed for 30 min at 65 °C using NEBNext Ultra II FS DNA Library Prep Kit (NEB, E7805S). The fragmented and dA-tailed DNA library was ligated to dsDNA adapter having both forward (Ligation FWD primer ...
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bioRxiv - Cell Biology 2023Quote: ... BarSeq PCR was performed using 200 ng of genomic DNA template in 150-μL reaction volumes with Q5 polymerase (with enhancer) (New England Biolabs, Ipswich, MA), 20 μM universal forward P5 primer ...
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bioRxiv - Molecular Biology 2024Quote: ... The PAGFP-TIRR plasmid was generated by amplifying a PAGFP fragment from a pPAGFP-C1 plasmid and a backbone containing TIRR using Q5® High-Fidelity DNA Polymerase and associated Q5® Reaction Buffer and GC enhancer (New England Biolabs). The PAGFP fragment was inserted C-terminally to TIRR.
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bioRxiv - Bioengineering 2020Quote: ... IVT was performed overnight at 37°C using the HiScribe T7 kit (NEB) following the manufacturer’s instructions (N1-methyl-pseudouridine modified) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then incubated at 45 °C for 2 hr with 5 μL of Proteinase K (NEB) in the presence of 40 mM EDTA (pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... ligation was performed during 4h at 22°C in the presence of truncated RNA ligase 2 (NEB)30 ...
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bioRxiv - Microbiology 2023Quote: ... and digested alongside pGS001 for 2 hours at 37°C using XhoI and XbaI (New England Biolabs). Digested plasmid backbone and PCR product were gel extracted (QIAquick Gel Extraction Kit ...