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Citations for New England Biolabs :
1 - 50 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... disulfide bonding enhancer 1 and 2 (New England BioLabs), RNase inhibitor (Takara Bio Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... elegans and mouse cDNA (PolyATtract® mRNA Isolation Systems, Promega and ProtoScript® First Strand cDNA Synthesis Kit, NEB). Detailed sub-cloning information is available upon request.
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bioRxiv - Molecular Biology 2023Quote: ... and inserted into the pGLuc Mini-TK 2 Gaussia luciferase enhancer reporter plasmid (NEB) via KpnI/SacI restriction sites ...
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bioRxiv - Genomics 2023Quote: ... 0.6 μL oxidation enhancer (NEB), 0.6 μL DTT (NEB) ...
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bioRxiv - Genomics 2023Quote: ... 0.3 μL oxidation enhancer (NEB), 0.3 μL DTT (NEB) ...
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bioRxiv - Microbiology 2019Quote: ... Q5 GC enhancer (New England Biolabs) was used for amplification of mobRP4 and tse2.
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bioRxiv - Immunology 2021Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer,10 mM dNTPs ...
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bioRxiv - Biochemistry 2021Quote: ... 1X Q5® GC Enhancer (NEB), 0.2 mM dNTPs ...
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bioRxiv - Immunology 2020Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer,10 mM dNTPs ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer,10 mM dNTPs ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer,10 mM dNTPs ...
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bioRxiv - Immunology 2023Quote: ... 5 µL of GC Enhancer (NEB), 5 µL of 5X buffer ...
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bioRxiv - Systems Biology 2023Quote: ... 5 μL High GC Enhancer (NEB), 0.5 μL 10 mM dNTPs ...
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bioRxiv - Bioengineering 2024Quote: ... including protein disulfide bond enhancer (NEB) and GamS (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer ...
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bioRxiv - Immunology 2024Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer,10 mM dNTPs ...
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bioRxiv - Neuroscience 2021Quote: ... elegans genomic DNA using Phusion DNA polymerase (New England Biolabs). nCre expression constructs containing these promoters and the unc-54 3’UTR were created using Gateway cloning (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μl ligation enhancer (New England BioLabs), 0.9 μl nuclease-free water and 0.1 μl duplexed adapter (100 μM ...
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bioRxiv - Genomics 2020Quote: ... 1 μl of NEBNext Ligation Enhancer (NEB), and 2 μl of 1.5 μM NEBNext adaptors for Illumina sequencing (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5× Q5 High GC Enhancer (NEB). PCR thermocycling conditions were 98 °C during 5 s ...
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bioRxiv - Genomics 2023Quote: ... 7 μL Q5 high GC enhancer (NEB), 0.07 μL 100mM dATP ...
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bioRxiv - Neuroscience 2019Quote: Enhancers were cloned from C57Bl/6J genomic DNA using enhancer-specific primers and Phusion high-fidelity polymerase (M0530S; NEB). Individual enhancers were then inserted into an rAAV or scAAV backbone that contained a minimal beta-globin promoter ...
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bioRxiv - Genomics 2022Quote: ... 10 µL of Q5 GC Enhancer Buffer (NEB), 2.5 µL of a forward primer (10µM) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X Q5 High GC Enhancer (New England Biolabs), 200 μM dNTP Solution Mix ...
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bioRxiv - Bioengineering 2024Quote: ... containing 0.06 % pluronic acid (F-127, final concentration) and 1 µL of each disulfide bond enhancer 1 and 2 (NEB #E6820). We used droplet oil consisting of 3M™ Novec™ HFE7500 Engineered Fluid (3M ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of GC enhancer buffer (New England Biolabs), 2.5 μL of the common reverse primer (BarSeq_P1 – Wetmore et al. ...
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bioRxiv - Genomics 2023Quote: Library oligos for the MYC enhancer screen were synthesized by Twist Bioscience and amplified using the NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L), forward primer ...
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bioRxiv - Microbiology 2020Quote: ... and Q5 hotstart polymerase with GC enhancer (New England Biolabs). Reactions were cycled as follows ...
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bioRxiv - Microbiology 2022Quote: ... we amplified the gene ctl0382 (homolog of ct127) with a six histidine tag in the reverse primer using Phusion enzyme (NEB), and the PCR product was linked with pBOMBL::L2 linearized by EagI and KpnI using HiFi assembly system (NEB) ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent pENTR clones were made by excising the original insert and replacing with new candidate enhancer fragments via Gibson Assembly (NEB HiFi Assembly Kit, New England Biolabs). The candidate enhancer fragments were shuttled to the transgenic vector pBPGUw [105] upstream of a minimal synthetic promoter sequence driving Gal4 using standard Gateway cloning techniques (LR Clonase ...
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bioRxiv - Molecular Biology 2021Quote: Site-directed mutagenesis of the AP-1 binding sites in the distal enhancer reporter plasmids were performed using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). Primer pairs 5’-CCATAATGTGgggCTATACTAAATTTCATCTTC-3’ and 5’-CTAAATCCACTTAGAAAAAACAATC-3’ ...
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bioRxiv - Neuroscience 2023Quote: ... the reporter and a barcode unique to each enhancer sequence using the NEBuilder® HiFi DNA Assembly Cloning Kit (NEB-E5520S), following standard protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and an additional 1 mM of Q5 enhancer (New England Biolabs Inc.) for trnLUAA-trnFGAA ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µL Q5® High GC Enhancer (New England BioLabs, Inc., B9028AVIAL), 0.5 µL Q5® High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Zoology 2019Quote: ... 10 µL NEB 5X Q5® High GC Enhancer (New England BioLabs, USA), 4 µL NEB Deoxynucleotide Solution Mix (10 mM each nt ...
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bioRxiv - Microbiology 2022Quote: ... using Q5 High-Fidelity DNA Polymerase with GC enhancer buffer (New England Biolabs). DYRK1A was cloned by Gibson assembly into pCW57.1-puro (gift of Katerina Politi ...
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bioRxiv - Microbiology 2024Quote: SV40 enhancer was amplified with PCR using Q5 High-Fidelity DNA Polymerase (NEB) from plasmid template ...
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bioRxiv - Synthetic Biology 2021Quote: ... elegans codon adaptor.[48] PCRs were carried out using Q5 2x Hot-Start Master Mix (New England Biolabs). PCR and digestion products were recovered from agarose gels following electrophoresis using the Zymogen Gel Recovery Kit (Zymo Research) ...
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bioRxiv - Cell Biology 2020Quote: ... elegans genomic DNA were digested in 80 µl total volume with 40 U HinfI (New England BioLabs, #R0155) and RsaI (New England BioLabs ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 37°C and purified using Monarch® DNA Gel Extraction Kit (T1020S, New England Biolabs) before digestion-ligation with the gRNA-pScaffold-H1 using FastDigest Esp3I and T7 Ligase (M0318L ...
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bioRxiv - Cell Biology 2023Quote: ... elegans osgn-1 (F30B5.4a, WormBase WS265) and human OSGIN1 were amplified by PCR using Phusion DNA polymerase (NEB, M0530L), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... using 35 cycles with an annealing temperature of 60 °C (see table 2 for list of primers) and cloned by Gibson Assembly Cloning Kit (EE5510S, NEB). All primers were designed using SnapGene (GSL Biotech LLC ...
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bioRxiv - Immunology 2021Quote: Point or deletion mutations were prepared from human or mouse CRT expression clones in pCMV3-C-Myc (SinoBiological) using the Q5® Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol and primers designed using the NEBaseChanger™ web tool ...
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bioRxiv - Molecular Biology 2019Quote: ... Enhancer variants were created using the inverse PCR method with Hot Start Q5 Polymerase (NEB) and primers available in Table 2 ...
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bioRxiv - Genomics 2020Quote: ... supplied with 5X Q5 Reaction Buffer and 5X High GC-Enhancer (NEB cat. no. M0491S)
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bioRxiv - Biochemistry 2023Quote: ... the library was amplified using Q5 High-Fidelity DNA Polymerases with GC enhancer (NEB, #M0491S) and subjected to size selection using a 6% polyacrylamide gel ...
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bioRxiv - Molecular Biology 2023Quote: ... All mRNAs were transcribed at 30°C for 2 hrs using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) and were co-transcriptionally capped (8:1 cap analog to GTP for ∼90% capping efficiency ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent pENTR clones were made by excising the original insert and replacing with new candidate enhancer fragments via Gibson Assembly (NEB HiFi Assembly Kit, New England Biolabs). The candidate enhancer fragments were shuttled to the transgenic vector pBPGUw [105] upstream of a minimal synthetic promoter sequence driving Gal4 using standard Gateway cloning techniques (LR Clonase ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...