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Citations for New England Biolabs :
51 - 100 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 30 µL of 10x buffer CutSmart (NEB), and 3 µL of BamHI-HF restriction enzyme (20,000 units/mL)] for 2 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... plus 30 units of RNase H (NEB) or 15 µL nuclease-free water at 37°C for 3 hr ...
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bioRxiv - Plant Biology 2022Quote: ... and Klenow Fragment (30/50 exo-) (NEB) were used for end repair and A-tailing ...
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bioRxiv - Biochemistry 2023Quote: ... and 30 units Hind III (NEB, R3104V) followed by resolved on a 0.8% agarose gel ...
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bioRxiv - Biochemistry 2022Quote: ... 5α Competent Escherichia coli (30 μl; NEB) was then transformed with 2 μl of the assembly reaction mixture by heat shocking for 30 s at 42 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 30 U/mL RNase inhibitor murine (NEB), 2.5 U/mL thermostable inorganic pyrophosphatase (TIPPase ...
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bioRxiv - Biophysics 2023Quote: ... and T4 ligase (30 U.μL-1, NEB) and incubated at 25°C for 2 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 30 µL of Q5 buffer (NEB #M0491L), and 65.5 µL of nuclease free water ...
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bioRxiv - Genomics 2020Quote: ... The PCR product and pGAD-C1 vector were digested with ClaI (5’-ATCGAT-3’, New England BioLabs Inc., MA, CA#R0197S) and SalI (5’-GTCGAC-3’ ...
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bioRxiv - Molecular Biology 2024Quote: ... complexes were produced by incubating sgRNAs (30 nM) and Cas9 nuclease (30 nM) in NEBuffer r3.1 (New England Biolabs) at room temperature for 10 min ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments ranging from 3 kb to 5 kb with 40-100 bp terminal homologies were amplified from mouse BAC RP23-51O13 with Q5 polymerase (NEB, M0491L). Approximately equal amount (100 ng ...
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bioRxiv - Biochemistry 2023Quote: ... The total RNA was treated by DNase I (5 U per 100 μg total RNA) and mRNAs were enriched by NEBNext Poly(A) mRNA magnetic isolation module (NEB E7490L). 8-10 μg of mRNA from each sample was fragmented to the size of ~120-nt by Ambion Fragmentation reagent (Thermo Scientific AM8740 ...
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bioRxiv - Biochemistry 2020Quote: ... m7G(5′)ppp(5′)G (NEB, #S1404S) and m7G(5′)ppp(5′)A (NEB ...
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bioRxiv - Genomics 2021Quote: ... 30 seconds at 65°C and 30 seconds at 72°C using the NEBNext® Multiplex Oligos kit (NEB, UK). The expected library size was ∼200-250bp ...
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bioRxiv - Genetics 2024Quote: We amplified 20 amino acid truncated poison from pSZB464 [15] with oligos 3183+3282 and cloned this into V08 [30][30] via Golden Gate assembly (New England Biolabs) to generate pSZB1444 ...
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bioRxiv - Biochemistry 2024Quote: ... 66 °C for 30 s, 72 °C for 30 s; and 72 °C for 2 min; Q5 Hot Start DNA polymerase [NEB]). PCR1 product was purified by SPRI beads (Mag-Bind TotalPure NGS ...
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bioRxiv - Microbiology 2020Quote: ... 30 μL of 10x RE buffer 4 (NEB), 2 μL of exonuclease T7 (10,000 units/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... 30 μL murine RNase inhibitor (NEB, Cat #M0314), 45 μL SuperScript™ III RT enzyme (200 U/μL ...
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bioRxiv - Genomics 2021Quote: ... 30 units of Phi29 polymerase (New England Biolabs), and molecular biology grade water to reach a final reaction volume of 50 μL ...
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bioRxiv - Immunology 2021Quote: ... coli cells (NEB, catalog no C3040H, 30°C). To generate gp70 C.1086 V1V2 RQ(H/Y)173 constructs ...
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bioRxiv - Genomics 2022Quote: ... and 30 nM recombinant Cas9 nuclease (NEB, M0386S) at 37°C for 2 hours ...
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bioRxiv - Bioengineering 2021Quote: ... and 30 units of BsaI-HF V2 (NEB) in a total volume of 35 µl per reaction ...
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bioRxiv - Microbiology 2021Quote: ... 30 units of DNase I (New England Biolabs), and 10 μl of concentrated protease inhibitor cocktail (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... the following 30 ul PURExpress IVTT reactions (NEB) were assembled ...
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bioRxiv - Systems Biology 2023Quote: ... all in a 30 µl rCutSmart buffer (NEB) reaction ...
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bioRxiv - Biochemistry 2024Quote: ... 30nM sgRNA and 30 nM Cas9 protein (NEB) for 1h at room temperature and analyzing cleavage products on a 2% agarose gel ...
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bioRxiv - Genomics 2022Quote: ... and 30 units Φ29 polymerase (New England Biolabs). Amplification conditions included a ramp-down from 35 to 31°C (5 min at 35°C ...
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bioRxiv - Biochemistry 2024Quote: ... 30 μL of Protein G Magnetic beads (NEB) was mixed with 1 μL of anti-RelA antibody (Diagenode ...
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bioRxiv - Molecular Biology 2022Quote: ... cell lysates were adjusted to the same protein concentration using furin cleavage buffer (100 mM Tris-HCL and 1mM CaCl2) and incubated with 5 units of Furin (NEB, Ipswich, MA) for 16 h at 30℃ ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg (final volume 100 μL) of the resulting mRNA was incubated with and without RNA 5’-pyrophosphohydrolase (RppH; NEB, 50 U) at 37° C for 1 hour in the supplied 1X buffer (72) ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was digested in a reaction with 30 nM single-guide RNA (sgRNA, Synthego) and 30 nM spCas9 (New England BioLabs, M0386S) after pre-incubation of the reaction mix at room temperature for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was digested in a reaction with 30 nM single-guide RNA (sgRNA, Synthego) and 30 nM spCas9 (New England BioLabs, M0386S) after pre-incubation of the reaction mix at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... and different 5′ cap structures of the viral genome were obtained by the addition of m7G(5′)ppp(5′)A cap analog or G(5′)ppp(5′)A cap analog (NEB). 2μg of transcript RNA was subsequently transfected into host cells seeded in a six-well plate with Lipofectamine MessengerMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and m7G(5′)ppp(5′)A (NEB, #S1405S) also referred throughout the manuscript as N7-meGpppG and N7-meGpppA for consistency ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µL RNase H (5 U/µL, NEB), 2.5 µL RNase III (1 U/µL ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-hydroxyl (5’HO-RNA30-FAM-3’) or 5’-Gppp (5’Gppp-RNA30-FAM-3’) in 1x NEBuffer 3 (NEB; B7003), in 20% denaturing polyacrylamide gels ...
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bioRxiv - Synthetic Biology 2021Quote: ... R3138S) and 30 U of NotI-HF (NEB, R3189) using CutSmart Buffer in a total reaction volume of 50 µL for fours at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... 30 blocked adaptor (Universal miRNA cloning linker, NEB S1315S) with 250 units of T4 RNA ligase truncated KQ (NEB M0373S) ...
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bioRxiv - Cell Biology 2020Quote: ... and 30 units of T7 DNA polymerase (NEB, M0274) to the annealed oligonucleotide–ssDNA mixture ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 units of Exonuclease III (M0206S, New England Biolabs), and either 10 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.3 μl T4 RNA ligase (30 U/µl; NEB). RNA samples were then subjected to nascent RNA extraction using Click-it nascent RNA capture kit (Thermo Fisher ...
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bioRxiv - Genomics 2022Quote: ... 30 units of T4 β-glucosyltransferase (New England Biolabs) and ultra-pure water in a final volume of 45 μL ...
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bioRxiv - Microbiology 2022Quote: ... 30 μg of Protein G magnetic beads (NEB #S1430) was washed with 250 μl of Immunoprecipitation Reaction Buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 μl T4 RNA ligase (30 U/µl; NEB), and 1.1 μl double distilled water ...
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bioRxiv - Molecular Biology 2024Quote: ... T4 RNA ligase high-concentration (NEB, M0437M, 30 U) and SUPERaseIn™ (5 U ...
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bioRxiv - Genomics 2024Quote: ... and 30 to 100ng was used for McrBC (NEB) overnight digestion (15hr) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 U T4 RNA ligase high-concentration enzyme (NEB), 5’ deadenylase enzyme (NEB ...
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bioRxiv - Genetics 2024Quote: ... then incubated with 30 U T4 DNA polymerase (NEB) at 12°C for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... 5 µl Taq 5× Master Mix (New England Biolabs) and 18 µl Milli-Q water (18.2 MΩ cm) ...
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bioRxiv - Developmental Biology 2024Quote: ... then capped with m7G (5’) ppp (5’) G (NEB) and tailed with a poly(A ...