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Citations for New England Biolabs :
51 - 90 of 90 citations for 18 0 PE square since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... a 2.7 kb genomic fragment corresponding to the MIR319C URR from the start of pre-MIR319C was amplified from Col-0 genomic DNA using Phusion High Fidelity DNA polymerase (M0530, New England Biolabs, USA) and cloned into modified P4P1R entry vector (a kind gift from Ram Yadav ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng total RNA in a volume of 9 μl was ligated to 1 μl custom adapter mix (18S:28S ratio 1:2) using 1.5 μl T4 DNA ligase (New England Biolabs) in NEB next Quick ligation buffer (3 μl ...
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bioRxiv - Microbiology 2021Quote: ... First strand cDNA synthesis was done by incubating 1 μg total RNA with 2.5 mM Oligo dT(18) and 0.5 mM dNTP mix (NEB, N0447L) for 5 min at 70°C before placing on ice for 2 min ...
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bioRxiv - Plant Biology 2022Quote: ... we first created a RUBY-minus vector lacking the gene GT by assembling PCR product 1 containing CYP76AD1 and DODA and PCR product 2 containing Arabidopsis HSP18.2 terminator into a pGFPGUSplus vector 18 via NEBuilder HiFi DNA Assembly (New England BioLabs). The pAXY0006 vector of split-RUBY was generated by assembling PCR products containing f1 fragment of gene GT (named GTf1 ...
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bioRxiv - Genetics 2024Quote: ... 1 μg of total RNA or 18S rRNA was degraded to single nucleotides using Nucleoside Digestion Mix (NEB #M0649), followed by the addition of a 4-fold volume of methanol and precipitation at −20°C for 2 hours to remove proteins ...
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bioRxiv - Biochemistry 2020Quote: ... NEB protocol #M0276 with incubation extended to 1 hour) and a Cap 0 analog (using a Vaccinia capping system, NEB protocol #M2080), following the recommended procedures ...
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bioRxiv - Neuroscience 2020Quote: ... Methylation standards (100 % and 0 %) were either prepared by in vitro methylation of human genomic DNA using M.SssI CpG methyltransferase (NEB, Frankfurt a.M; Germany) or by whole-genome amplification using the REPLI-g Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... from Col-0 cDNA and prepared for in vitro transcription with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). After purification with Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... and on the other side a primer that targets a region introduced through the library preparation called “Read 1” (18 cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs)) ...
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bioRxiv - Neuroscience 2020Quote: ... either purified directly (insert) or from agarose gel (vector) and ligated (16-18 h, 20-22 °C) with T4 DNA ligase (NEB) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... mutants of UFD1-His6 (deletion of amino acids 2-215) and NPL4 (T590L+F591V point mutations) 18 were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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bioRxiv - Neuroscience 2022Quote: ... was linearized for 18 hours at 37°C in a 250 μl reaction containing restriction enzyme NheI and CutSmart buffer (New England Biolabs). The linear DNA plasmid was next used to produce complementary RNA (cRNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR-enrichment was performed in 18 cycles in ten 10μL reactions per sublibrary with Phusion® HF PCR Mastermix (New England BioLabs) by mixing 5µl 2× Phusion Master mix ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was clarified by centrifugation at 5°C at 18 500 rpm for 30min and loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB1_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... to the branch point recognition sequence (Nucleotides 18-42) of U2 snRNA and to GAPDH mRNA were added in combination with RNase H (NEB) to NE ...
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bioRxiv - Immunology 2022Quote: ... Sample index PCR: 2 µl tagging PCR product was mixed with 18 µl PCR mix (4 µl 5x phusion HF buffer (NEB); 0.4 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Genomics 2024Quote: ... 4.0 μg of the pool DNA was repaired and end-repaired using NEBNext FFPE DNA Repair Mix (New England BioLabs, Ipswich, MA, USA) and the NEBNext UltraII End Repair/dA-Tailing Module (New England BioLabs ...
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bioRxiv - Developmental Biology 2020Quote: Alg2 cDNA was amplified with RT-PCR from the cDNA of wt Oryzias latipes Cab strain stage 18 embryos with Q5® High-Fidelity DNA Polymerase (New England Biolabs) by using primers with BamHI-HF (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA fragments were inserted into PCR-amplified (primers 17-18 in Table S3) and purified pRSET vector fragment by Gibson Assembly (NEB, USA) to add sequence parts necessary for cDNA display generation to the DNA library ...
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bioRxiv - Systems Biology 2021Quote: Rho libraries were amplified using primers MO574 and MO575 (Supplementary file 6) for 6 cycles at an annealing temperature of 66C followed by 18 cycles with no annealing step (NEB Phusion) and then purified with the Monarch PCR kit (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 18 hours and the plasmid was extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was denatured and annealed in an 18 μl reaction (15 μl PCR product, 2 μl NEB Buffer2 (10X), 1 μl nuclease-free H2O ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 20 µl of distilled water and mixed with 18 µl gel loading dye (New England Biolabs, Inc.). Samples were resolved in a 10% SDS-polyacrylamide gel ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated with 25 μL of containing TF or 18S primers and fluorescent Luna® Universal qPCR Master Mix (New England Biolabs), and qRT-PCR was carried out in triplicate for each sample on the CFX Connect real-time System (Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2024Quote: ... This DNA was subjected to 18 cycles of PCR using whole genome primers (ONT #SQK-PSK004) and LongAmp Hot Start Taq 2X Master Mix (NEB #M0533). Library preparation was done using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645 ...
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bioRxiv - Genomics 2024Quote: ... Ligation was performed by addition to the sample of Ligation master mix (2007 μl of water, 300 μl of 10% Triton X-100, 18 μl of 10 mg/ml BSA (NEB, B9000S), 360 μl 10x T4 DNA ligase buffer (NEB ...
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bioRxiv - Microbiology 2024Quote: ... DNA sequences were amplified and amended with ≥18 bp homology to their destination vectors using Q5 Hot Start High Fidelity Master Mix (NEB, M0494L). Vectors were constructed using Gibson Assembly with HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2024Quote: Small RNA sequencing libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following the manufacturer’s recommendations with exclusion of the initial size selection and the 3’ ligation step changed to 16°C for 18 hours to improve capture of methylated small RNAs (New England Biolabs, E7300S). Small RNA PCR products were size selected on a 10% polyacrylamide gel ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The supernatant containing the immunoprecipitated DNA was collected and amplified through 18 PCR cycles using whole genome primers (ONT #SQK-PSK004) and LongAmp Hot Start Taq 2X Master Mix (NEB #M0533). The final library was prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645 ...
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bioRxiv - Microbiology 2020Quote: ... pCrPV-1A-DcDV was linearized by inverse PCR with primers 17 and 18 and the linearized plasmid was circularized by blunt end ligation with T4 DNA ligase (New England Biolabs, Ipswich, Massachusetts) according to the manufacturer’s instructions to create pCrPV-1A-DcDV-1A ...
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bioRxiv - Genomics 2024Quote: ... Small RNA sequencing libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following the manufacturer’s recommendations with the 3’ ligation step changed to 16°C for 18 hours to improve capture of methylated small RNAs (New England Biolabs, cat# E7300S). Small RNA PCR amplicons were size selected on 10% polyacrylamide non-denaturing gels ...
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bioRxiv - Plant Biology 2023Quote: ... The YFP-CESA6 construct was used as a template to prepare 18 YFP-CESA6 mutated constructs using a Q5 Site Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) with the specific primers listed in Supplemental Table S4 ...
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bioRxiv - Molecular Biology 2022Quote: ... The pCMV-PE4max with MluI restriction site and the pLV-PE plasmid were both digested by MluI (NEB #R0198L) and NotI (NEB #R0189L) ...
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bioRxiv - Microbiology 2020Quote: ... nahA encoding sequence was PCR amplified and incorporated into pE-SUMO vector by Golden Gate assembly method (New England BioLabs), and into pVL847 (His-MBP-tag overexpression vector ...
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bioRxiv - Biochemistry 2020Quote: ... was produced by cloning five synthetic gene fragments in pE-SUMO (Life Sensors, PA) using NEBuilder HiFi DNA Assembly (New England Biolabs). For purification ...
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bioRxiv - Microbiology 2023Quote: ... The 5’ end ligated samples were purified using PCI extraction and then the 3’ App-PE adapters were ligated to the RPF using 40 U T4 RNA ligase I (NEB). The 5’ and 3’ ligated samples were resolved on a 12% TBE-Urea polyacrylamide gel and the band corresponding to the RPF was gel-excised ...
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bioRxiv - Microbiology 2023Quote: ... The 32P-radioabelled RNase III.RNA complexes were washed and unique barcoded 5’ linkers and 3’ App-PE adapters were ligated to the bounded RNA using 40 U T4 RNA ligase I (NEB) for each ligation step ...
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bioRxiv - Genomics 2024Quote: ... The PE RNA sequencing libraries were prepared using the NEBNEXT Ultra RNA Library Prep Kit for Illumina (New England Biolabs Inc.,USA) and 150 bp PE sequencing was performed on Illumina Hiseq X Ten platform ...
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bioRxiv - Plant Biology 2022Quote: ... A total of 0.5 μg of total RNA per biological replicate were used for preparing 150 bp paired-end (PE) read libraries using the NEBNext®Ultra™ II RNA Library Prep Kit (New England Biolabs, Inc.). Small RNA was extracted using a Plant miRNA kit (Omega Bio-tek Inc.) ...
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HTLV-1 Splice Sites in Prevalent Gene Vectors Cause Splicing Perturbations in Transgenic Human CellsbioRxiv - Genomics 2023Quote: ... Following magnetic bead purification one third of the cDNA per sample was subjected to PCR amplifications with the primers HTLV-1 and PE-2nd-GA using the NEBNext® Ultra™ II Q5® Master Mix (NEB) using the cycling program ...