Labshake search
Citations for New England Biolabs :
1 - 50 of 90 citations for 18 0 PE square since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... DNase I (NEB; 18 U), and lysozyme (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2020Quote: ... 18 μL 25 mM dNTPs (NEB), 30 μL 0.1 M DTT (Invitrogen) ...
-
bioRxiv - Biochemistry 2020Quote: 18 µg HindIII-digested lambda DNA (NEB N3012S) was de-phosphorylated with 2 μL of Calf Intestinal Phosphatase (NEB M0290S ...
-
bioRxiv - Microbiology 2024Quote: ... and digested for 18 hours with SbfI-HF (NEB). The gRNA cassette was isolated by gel electrophoresis with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
-
bioRxiv - Molecular Biology 2022Quote: ... some 18S rRNA samples) using truncated T4 RNA ligase K227Q (NEB) to protect the 3’-end of the rRNAs from degradation and to identify the true rRNA 3’-end after sequencing (Table S3) ...
-
bioRxiv - Plant Biology 2021Quote: ... was amplified from Col-0 cDNA using Q5 High-Fidelity DNA Polymerase (NEB), as recommended by the manufacturer ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA was generated using oligo (dT) 18 primer (New England Biolabs, 513165) and SuperScript II Reverse Transcriptase (Invitrogen ...
-
bioRxiv - Plant Biology 2021Quote: ... TAC clone JAtY79I19 obtained from Arabidopsis accession Col-0 was digested with KpnI (NEB) and SalI (NEB) ...
-
bioRxiv - Molecular Biology 2024Quote: Level 0 entry vectors were created through a scaled down NEBuilder (NEB, Ipswich, MA) reaction ...
-
bioRxiv - Molecular Biology 2021Quote: ... with a 1:1 combination of oligodT 18 primers and random hexamers (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified DNA material was PCR-amplified (18 cycles) using Phusion polymerase (NEB) and purified by MinElute PCR purification kit (Qiagen).
-
bioRxiv - Microbiology 2020Quote: ... Phage stocks were treated with 18 U DNase I (New England Biolabs, M0303) and 0.1mg RNase A (ThermoFisher ...
-
bioRxiv - Genetics 2021Quote: ... PCR was limited to 18 cycles using Phusion polymerase (New England Biolabs, M0530). Mutagenesis PCR product was then digested overnight using DpnI (New England Biolabs ...
-
bioRxiv - Plant Biology 2022Quote: ... along with oligo-dT 18 mer primer (New England Biolabs, Ipswich, MA; www.neb.com) was used to synthesize cDNA ...
-
bioRxiv - Immunology 2020Quote: ... to generate Cap 0 and in some cases also the Vaccinia 2’ O methyltransferase (NEB) to generate Cap 1 ...
-
bioRxiv - Microbiology 2023Quote: ... 5 μl 10 mM MnCl2 buffer and 0 or 2 μl lambda phosphatase (NEB #P0753S, USA) in 38 μl supernatant ...
-
Phase separation-based visualization of protein-protein interactions and kinase activities in plantsbioRxiv - Plant Biology 2022Quote: ... pET-SOMA 48 and Arabidopsis Col-0 cDNA by using the Q5 DNA polymerase (NEB®). The resulting PCR fragments were redeemed via column purification ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The Level 0 plasmid was amplified with the primer pair SLo0765/SLo0766 followed by DpnI (NEB) digestion prior to ligation (any Level 0 plasmid will do ...
-
bioRxiv - Plant Biology 2020Quote: ... RGA::dRGA was amplified from genomic DNA of Col-0 using Q5 high fidelity DNA polymerase (NEB). Two overlapping fragments lacking 17 aminoacids covering the DELLA domain like described in (Feng et al ...
-
bioRxiv - Molecular Biology 2021Quote: ... EcoRI sites underlined) and different concentration of recombinant His-SET8 protein (0 to 1.4 μM, New England Biolabs) were incubated for 10 min on ice in 1× GRB binding buffer [20 mM HEPES pH 7.5 ...
-
bioRxiv - Plant Biology 2022Quote: ... the At5g20040 transcription unit was amplified from Col-0 cDNA using the Q5 High-Fidelity DNA Polymerase (NEB), as recommended by the manufacturer using the oligonucleotides attB_IPT9_F and attB_IPT9_R ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The level 0 acceptor plasmid was amplified with the primer pair SLo0765/SLo0766 using Q5 DNA Polymerase (NEB) followed by a DpnI (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... pET20b::bepA::6xHis vector was used in 18 cycles of PCR using the Phusion polymerase (NEB) as described by the manufacturer ...
-
bioRxiv - Genetics 2021Quote: PE-IVT: The EnGen sgRNA Synthesis Kit (New England BioLabs E3322) was used for PE-IVT per the kit protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... Promoters of ABCG17 and ABCG18 were amplified from Col-0 DNA using Phusion High-fidelity Polymerase (New England Biolabs) using primers listed in Supplementary Table 3 ...
-
bioRxiv - Genomics 2023Quote: ... each were incubated in parallel with 100 U or 0 U of M.CviPI (Xu et al. 1998) (New England Biolabs) in methylation buffer (320 µM S-adenosyl-L-methionine in CRB ...
-
bioRxiv - Plant Biology 2021Quote: ... and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA Polymerase (New England Biolabs). Libraries were pooled and sequenced (paired-end 2×150bp ...
-
bioRxiv - Biophysics 2021Quote: ... Complexes were prepared by incubating spike ectodomain preparations with either ACE2 (residues 18–615, New England Biolabs), VH ab8 ...
-
bioRxiv - Microbiology 2022Quote: ... the above construct was amplified using primers 17 and 18 and integrated into BamHI/PacI-digested (NEB) pUPRT plasmid by Gibson assembly ...
-
bioRxiv - Synthetic Biology 2024Quote: ... approximately 40 µg of pSC5 was digested with 18 µl of SacI-HF (New England Biolabs, R3156) at 37 °C for 1 hour ...
-
bioRxiv - Biochemistry 2022Quote: ... the empty pE-SUMOpro Amp vector was cut using BsaI enzyme (NEB). ORFs were amplified with primers that added 5’-CGCGAACAGATTGGAGGT-3’ upstream of the start of the ORF ...
-
bioRxiv - Plant Biology 2021Quote: ... and including the endogenous stop codon was amplified from Arabidopsis Col-0 genomic DNA using Phusion Taq polymerase (New England Biolabs) and cloned into pENTR using the D/Topo kit (Invitrogen) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and 0.2 μl 50x oligos (AarI recognition site) for Level 0 and Level 2 cloning or Eco31I/BsaI-HFv2 (ThermoFisher Scientific/NEB) for Level 1 cloning ...
-
A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeastbioRxiv - Genomics 2020Quote: ... Amplified guide RNAs were cloned in a 100 μl assembly reaction with 1.0 μg linearized pNTI661 and 1.7 μl guide RNA PCR using 2 × NEBuilder HiFi DNA Assembly Master Mix (NEB E2621L), which was incubated for 1 hour at 50 °C and then purified with a DNA Clean & Concentrator with final elution into 10 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.52 and 0 nM) in a final volume of 30 μL of amplification mixture including: 1X Phi29 DNA polymerase buffer (NEB), 0.3 μM non-modified PCR forward and reverse primers ...
-
bioRxiv - Molecular Biology 2022Quote: Full-length CDS of candidate RBPs and DNA fragments covering the RBDs were PCR amplified from cDNAs reverse-transcribed from Col-0 mRNA using the Phusion DNA Polymerase (NEB), and cloned into the pMCSG9 vector using the ligation-independent cloning (LIC ...
-
bioRxiv - Plant Biology 2022Quote: ... and 100 ng of bending site RNA samples from five-day-old Col-0 and myb93 plants using the NEBnext Ultra II RNA Library Prep kit (New England Biolabs) following manufacturer protocols ...
-
bioRxiv - Plant Biology 2023Quote: ... Promoters and 3’ UTRs were amplified from Col-0 genomic DNA using the primers listed in (Supp Table 3) with Q5 Hot Start High-fidelity DNA polymerase (NEB). For MBD5 and SUVH3 ...
-
bioRxiv - Developmental Biology 2024Quote: ... the AT1G65620/AS2 coding sequence (600 bps) was amplified from Col-0 cDNA using primers oLH111 and oLH112 by Phusion PCR (NEB), and cloned into pENTR™/D-TOPO™ (Invitrogen™) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and 0.2 μl 50x oligos of the AarI recognition site for Level 0 and Level 2 cloning or Eco31I/BsaI-HFv2 (ThermoFisher/NEB) for Level 1 cloning ...
-
bioRxiv - Plant Biology 2024Quote: ... and VAMP711a were amplified from the cDNA of Col-0 Arabidopsis plants using Phusion High-Fidelity DNA Polymerase (New England Biolabs) and primers described in (Supplementary Table S2) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... These synthesized fragments were assembled into the Level 0 entry vector pYTK001 using the NEBridge Golden Gate Assembly Kit (BsmBI-v2) (New England Biolabs) following the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2024Quote: ... coli genome was digested in the supernatant and the suspension with 0-10 U of exonuclease V (RecBCD) (New England Biolabs). The reaction was carried out in a 10-μL volume at 37°C for 1 h ...
-
bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared with NEBnext Ultra DNA Library Prep Kit (New England Biolabs; version 6.0 – 2/18) using NEBNext Multiplex Oligos for Illumina-Dual Index Primers Set 1 (#E7600S ...
-
bioRxiv - Molecular Biology 2022Quote: ... Then 50 μl of DNase I mixture (25 μl of 10× DNase I buffer, 18 μl of NEB DNase I ...
-
bioRxiv - Cancer Biology 2020Quote: ... HiC DNA was amplified using PE PCR primers and Phusion DNA polymerase (NEB) for 7-9 cycles ...
-
bioRxiv - Genomics 2020Quote: ... 2 mM EDTA pH 8.0) was performed to obtain 40-70-nucleotide fragments that were subsequently size-selected and dephosphorylated with T4 PNK (NEB, M0201S) for ligation of a 3’ DNA linker containing a random hexamer molecular barcode to allow detection of PCR duplicates84 ...
-
bioRxiv - Plant Biology 2023Quote: ... a 2.7 kb genomic fragment corresponding to the MIR319C URR from the start of pre-MIR319C was amplified from Col-0 genomic DNA using Phusion High Fidelity DNA polymerase (M0530, New England Biolabs, USA) and cloned into P4P1R to generate the pMIR319C-P4P1R construct ...
-
bioRxiv - Plant Biology 2022Quote: ... gene with stop codon was amplified from wildtype Col-0 cDNA by Phusion High-Fidelity DNA Polymerase (New England Biolabs, #M0530L) and cloned into the pENTR plasmid (Invitrogen) ...
-
bioRxiv - Plant Biology 2024Quote: ... The generated level 0 tRNA-sgRNA modules were then combined via another cut-ligation reaction using BsaI-HF®v2 (New England Biolabs) into two L1 transcription units (C441001 and C441002 ...