Labshake search
Citations for New England Biolabs :
9801 - 9850 of 10000+ citations for Human Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... using Custom Nextera PCR primer and the NEBNext High-Fidelity 2x PCR Master Mix (Nextera DNA Library Preparation Kit (New England BioLabs, E7530L) and purified with the Qiagen MinElute Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Three independent sequencing libraries for each genotype were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). Illumina NovaSeq 6000 was used for sequencing ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: 500 ng of RNA was processed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB) with prior enrichment of mRNA by using Oligo dT beads from the NEBNext Poly(A ...
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bioRxiv - Genomics 2023Quote: ... E7490) and NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Inc., Cat. No. E7420). All libraries were dual-indexed using 12 cycles of PCR amplification using NEBNext® Multiplex Oligos for Illumina® Dual Index Primer Set (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... DNA was used to prepare a short reads library using NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs, England) and sequenced on Illumina HiSeq X instrument (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... 125 ng of fragmented DNA was used to prepare each DNA library following the NEBNext Ultra II library prep kit for Illumina (NEB, Inc.) until the loop-adaptor ligation step ...
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bioRxiv - Biophysics 2023Quote: ... PUS1D134A lacking the N-terminal HIS-tag was subcloned into expression vector pET21d (EMD Biosciences) using a Gibson Assembly cloning kit and protocol (NEB # E5510S) and sequence verified ...
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bioRxiv - Molecular Biology 2023Quote: ... All mRNAs were transcribed at 30°C for 2 hrs using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) and were co-transcriptionally capped (8:1 cap analog to GTP for ∼90% capping efficiency ...
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bioRxiv - Genomics 2023Quote: The template genomic DNA was fragmented by sonication to an insert size of 350 bp using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample and sequenced using an Illumina Hiseq 2500 PE150.
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bioRxiv - Genomics 2022Quote: ... Library preparation on samples was conducted using manufacturer’s protocols for NEBNext Ultra II Dna Library Prep Kit (New England BioLabs, CAT#E7645) and NEB Multiplex Dual Index oligos (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation module (NEB #E7490). Libraries were sent to Novogene for Illumina Hi-Seq ...
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bioRxiv - Genomics 2023Quote: Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, cat. No E7645S and E7335S) and amplified using 14 PCR cycles following the manufacturer’s instructions with slight modifications as previously described by us in detail elsewhere25 ...
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bioRxiv - Developmental Biology 2023Quote: ... This plasmid was modified to include a C-terminal V5 tag using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For arnt1-myc ...
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bioRxiv - Biochemistry 2023Quote: ... A linear expression cassette of pD912-PGAP-Hp AMPD-His10 was assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). Primers were designed using the NEBuilder Assembly Tool ...
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bioRxiv - Genetics 2023Quote: ... cDNA and sequencing libraries were generated from 500 ng of fresh RNA samples with 10 cycles of PCR with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760). After quality checking using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was normalized for cDNA conversion using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Massachusetts, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of total RNA was used as a template for cDNA synthesis with the LunaScript® RT SuperMix Kit (#E3010, New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... using a minimum of 20 bp overlapping regions between DNA fragments with custom made kit Plasmid selection and verification after the transformation were checked using the OneTaq® 2X Master Mix with Standard Buffer (NEB). Plasmids were purified using the Qiagen plasmid purification kit according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... was modified to introduce BsaI cut site and single sgRNAs were cloned into it by using Golden Gate Assembly kit (BsmBI-v2, New England Biolabs #E1602). All single sgRNAs used in this study are listed in Supplementary Table 5 ...
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bioRxiv - Biochemistry 2023Quote: ... A 2 µL aliquot of the annealed oligonucleotides was used as template for in vitro RNA transcription by HiScribe T7 quick high yield RNA Synthesis kit (New England Biolabs, MA). The transcribed RNA was purified using RNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... Library preparation was done according to NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs) including polyA enrichment and addition the of ERCC RNA spike-ins ...
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bioRxiv - Microbiology 2023Quote: High molecular weight DNA was extracted from isolate OI25 and its revertants using the Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs) according to the manufacturer’s protocol with the addition of 50 μg/ml lysozyme (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The 2X FLAG sequence was introduced via PCR as an oligonucleotide primer along with a reverse primer that produced the 3’ CNA1 UTR and cloned by use of the Gibson Assembly Cloning Kit (NEB #E5510S). The identity of the vector pCnat-CNA1-2X FLAG was also confirmed by sequencing.
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bioRxiv - Microbiology 2023Quote: ... Sequence libraries were prepared with the NEBNext Ultra II FS DNA Library Prep 136 kit (New England Biolabs GmbH, Frankfurt, Germany) and sequenced with the NovaSeq6000 (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was used to prepare poly-A selected directional libraries using the NEB Next Ultra Directional RNA Library Prep Kit (New England BioLabs, USA) and libraries were sequenced utilizing the Illumina Hiseq2000 platform at the sequencing core facility of the Max Planck Institute of Immunology and epigenetics (Freiburg ...
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bioRxiv - Molecular Biology 2023Quote: ... peptide sequences specific to an aMino sdAb were generated using the Ph.D.™-C7C Phage Display Peptide Library Kit (New England BioLabs, E8120S), a combinatorial library consisting of randomized display peptides with a disulphide constrained loop (AC-XXXXXXX-CGGGS ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously 45.
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bioRxiv - Molecular Biology 2023Quote: ... Up to 0.5ng of DNA from KTRC donor samples (3399, 3409, 3447) was used to prepare sequencing libraries using NEBNext Ultra II Library Kit (NEB, E7645), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The Lysobacter gasdermin system was previously cloned10 and the Pedobacter rhizosphaerae system was amplified from genomic DNA ordered from the DSMZ (DSM 18610, IMG gene accessions in Table S2) and cloned using the NEBuilder HiFi DNA Assembly cloning kit (NEB E5520S). As a negative control ...
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bioRxiv - Bioengineering 2023Quote: ... Start codon of MAAP-WT2 (CTG) was substituted with ATG using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) in accordance with manufacturer’s protocol.
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bioRxiv - Biophysics 2023Quote: ... The R203M N175-245 plasmid was prepared from the WT N175-245 plasmid with site directed mutagenesis (NEB site mutagenesis kit). The plasmids were transformed into BL21(DE3 ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA libraries from at least three replicate individuals per sex for each lineage (Table S1) were prepared from gametes using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs). Mature gametophytes release gametes just after the medium renewal ...
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bioRxiv - Plant Biology 2023Quote: ... The purified total RNA was used to synthesize cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, catalog number E3010).
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bioRxiv - Neuroscience 2023Quote: ... the reporter and a barcode unique to each enhancer sequence using the NEBuilder® HiFi DNA Assembly Cloning Kit (NEB-E5520S), following standard protocol ...
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bioRxiv - Microbiology 2023Quote: ... and the appropriate crRNA gBlock (IDTDNA; Coralville, IA) was inserted using the NEBuilder HiFi DNA assembly kit (New England Biolabs (NEB); Cambridge ...
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bioRxiv - Microbiology 2023Quote: ... Region of Cori and ter were quantitatively amplified using the Sybr green-based Luna Universal One-step RT-qPCR kit (New England Biolabs Inc.), without the reverse transcriptase enzyme ...
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bioRxiv - Genomics 2023Quote: ... and strand-specific sequencing libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, E7760S) with 8 cycles of amplification ...
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bioRxiv - Genomics 2023Quote: 50μl of size selected DNA was used as the start material for library generation using NEBNext Ultra II DNA library preparation kit for Illumina (NEB E7645S). The end preparation (end repair and A tailing ...
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bioRxiv - Plant Biology 2023Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Molecular Biology 2023Quote: ... A total of 3 - 10 × 106 EB-derived CD71+ were processed per biological replicate and libraries of sufficient quality were indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645) according to the manufacturer’s instructions ...
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Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... sequencing libraries were generated using the rRNA-depleted RNA by NEBNext UltraTM Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc.) with AMPure XP (Beckman Coulter Life Sciences ...
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bioRxiv - Genomics 2023Quote: MChIP-C NGS libraries were prepared from immunoprecipitated DNA with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... adapter ligation and PCR amplification (9 cycles) was performed using NEBNext Ultra II directional RNA library prep kit for Illumina protocol (NEB, E7760) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... oligonucleotides corresponding to each CTCF element were cloned into the pROSA-TV2 vector (created by Prof. Benjamin Davies group) between the pair of BsaI sites using the Golden Gate Assembly Kit (NEB, E1601) and this generates the HDR donor template containing the inserted CTCF element ...
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bioRxiv - Cancer Biology 2023Quote: ... rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB, E7760L) by following manufacture’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Positive colonies were incubated overnight at 37°C and plasmids were isolated using the Monarch Plasmid Miniprep Kit (New England Biolabs, T1010L). For all assembled plasmids the sequence was confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 µl of the PCR-amplified product was employed for in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), with incubation at 37 °C for 18 hours in a thermocycler ...
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bioRxiv - Molecular Biology 2023Quote: ... of extracted RNA (either from whole-cell lysates or sucrose gradient fractions) was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB E6560L) according to the manufacturer instructions with some modifications ...
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bioRxiv - Neuroscience 2023Quote: ... was used to construct sequencing libraries using the NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs; #E7760). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers ...