Labshake search
Citations for New England Biolabs :
9501 - 9550 of 10000+ citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The immunoprecipitated DNAs were subsequently processed to generate a DNA library using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, # E7645L) and NEBNext® Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2022Quote: ... night RNAseq libraries were produced according to the manufacturer’s protocol (NEBNext UltraII Directional RNA Library Prep Kit, Illumina, NEB, MA, USA) using aroung 1 000 ng of total RNA ...
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bioRxiv - Microbiology 2022Quote: ... The viral cDNAs were synthesized from extracted RNA using the Luna Script RT Super Mix Kit (New England BioLabs, Ipswich, MA), followed by DNA amplification by multiplex PCR in two separated primer pools using ARTIC-N5 primers (59 ...
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bioRxiv - Immunology 2022Quote: ... Caspase-11 catalytic (C254A) and cleavage (D285A) mutants were generated by site-directed mutagenesis (Q5 SDM Kit, New England BioLabs; #E0554S) of the wild-type parent vector according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1-step RT-qPCR was performed on 2μL of RNA using the Luna® Universal One-Step RT-qPCR Kit (NEB Biosciences) and primers for β-tubulin (F ...
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An apical protein, Pcr2, is required for persistent movement by the human parasite Toxoplasma gondiibioRxiv - Cell Biology 2022Quote: ... and inserted between the BglII and AflII sites of ptubA1-mEmeraldFP-TUBA1[69] using the NEBuilder HiFi Assembly kit (New England Biolabs, #E2621S).
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously (33) ...
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bioRxiv - Molecular Biology 2022Quote: ... for each co-expressed DF-APOBEC1 protein using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) with NEBNext Poly(A ...
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bioRxiv - Developmental Biology 2022Quote: ... equal amount of DNA (∼2 ng) was used as an input for NEB Ultra II DNA library prep kits (NEB #E7645). Number of cycles for amplification of adapter ligated libraries were estimated by the qPCR before final amplification to avoid any bias arising due to PCR amplification and indexing (NEB #E7350) ...
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bioRxiv - Biochemistry 2022Quote: ... and for OGA we introduce a D174>A mutation with a Q5 site-directed mutagenesis kit (New England Biolabs cat # E05545). These mutations produce stable mutant proteins that abolish the catalytic activity of the enzymes (38,39) ...
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bioRxiv - Cancer Biology 2022Quote: In vitro transcription of LINC00313 or firefly luciferase (F-luc) mRNA was performed using the HiScribe™ T7 High Yield RNA Synthesis kit (New England Biolabs), according to the instructions by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... RNA was used for RNA-Seq library generation using the NEBNext Ultra II Directional Library Prep kit for Illumina (NEB, E7760S). Amplified cDNAs were submitted for Illumina sequencing.
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bioRxiv - Biochemistry 2022Quote: ... RNA from each sample was used for RNA-Seq library generation using the NEBNext Ultra II Directional Library Prep kit for Illumina (NEB, E7760S). Amplified cDNAs were submitted for Illumina sequencing.
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bioRxiv - Biochemistry 2022Quote: ... GT GGT ATT GCC GAC GGA G) and the combination of both were generated using the Q5® site-directed mutagenesis kit (NEB) (lower case letters highlight the amino acid change) ...
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bioRxiv - Bioengineering 2022Quote: ... constant oligonucleotide was used.(Petri et al., 2022) The products were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs), which were then used as the template for in vitro transcription ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was carried out with a standard oligo-dT primer protocol using the ProtoScript II First Strand cDNA Synthesis Kit (NEB E6560). RNA concentrations were normalized between samples prior to reverse transcription ...
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bioRxiv - Developmental Biology 2022Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Briefly 500pg of ribozero treated RNA was fragmented and used to produce cDNA libraries using the NEBnext Ultra RNA library prep kit (NEB, E7530S) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Library quality was assessed using an Agilent BioAnalyzer 2100 and libraries were quantified with the Library Quant Kit for Illumina (NEB #E7630L). Libraries were then diluted to 1.8pM and sequenced on the NextSeq500 platform using 75-base-pair (bp ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... An IFT144 construct lacking the N-terminal β-propeller domain (residues 2–349 inclusive; IFT144ΔNFLAG) was made using the Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Cancer Biology 2022Quote: ... and library preparation was done using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA).56 Paired-end 150 bp read sequencing was performed ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs)57,58 or via QuickChange (Q5® Site-Directed Mutagenesis Kit from New England Biolabs).59 Recombinant expression of motors in E ...
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bioRxiv - Immunology 2022Quote: ... Short hairpin resistant chimeric mutants of Flag-tagged GLUT3 alleles were generated using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... were used for RNAseq library preparation with the NEBNext Single Cell / Low Input RNA Library Prep Kit for Illumina (NEB, E6420S), following the manufacturer’s protocol for single cells ...
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bioRxiv - Genetics 2022Quote: ... and DNA library preparation was performed using NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads Kit (NEB, #E6177). Whole-genome sequencing was performed using an Illumina NextSeq instrument ...
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bioRxiv - Evolutionary Biology 2022Quote: ... One µg of total RNA was used for the NEBNext® Ultra™ Directional RNA Library Prep Kit (New England Biolabs) without removing ribosomal RNA ...
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bioRxiv - Genetics 2022Quote: ... Libraries were produced with the Ultra II DNA Library prep kit according to manufacturer’s instructions (New England Biolabs, cat. no. E7645L). Bisulfite conversion of DNA was carried out according to manufacturer’s protocol (Zymo ...
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bioRxiv - Immunology 2022Quote: ... mRNAs encoding TCR genes were transcribed in vitro using the mMESSAGE mMACHINE® T7 Ultra Kit (Life) using the Avr□ (NEB) linearized plasmid DNA as templates ...
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bioRxiv - Developmental Biology 2022Quote: ... Indexed libraries were made using 0.5 μg RNA per sample with NEBNext Ultra™ RNA Library Prep Kit for Illumina with the Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Genetics 2022Quote: ... 500 ng of purified PCR products (D2500, Gel Extraction Kit, OMEGA, USA) were denatured and reannealed in NEB buffer 2 (M0302S, NEB, USA): 95 ℃ ...
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bioRxiv - Genomics 2022Quote: ... IPs and INPUTS were then quantified and NGS libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, #E7645) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... from ONTs ligation sequencing kit (ONT, SQK-LSK109) and 10 µl of NEBNext Quick T4 DNA Ligase (New England Biolabs, E6056S). Ligation mix was incubated at RT for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... and single amplicon copies were ligated into the pMiniT 2.0 vector using the NEB PCR Cloning Kit (New England Biolabs, Ipswich, MA). Individual clones were selected and confirmed via colony PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... followed by treatment with DNase I.5 The samples were then purified with the Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2022Quote: crRNAs were synthesized for in vitro cleavage experiments using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs; NEB). Templates for in vitro transcription were generated by hybridizing a ssDNA oligo with the T7 promoter to another oligo with the complementary T7 promoter sequence fused to the LbCas12a repeat and spacer in annealing buffer ...
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bioRxiv - Microbiology 2022Quote: ... was used as a template to prepare 10 μl cDNA using LunaScript®RT SuperMix Kit (New England Biolabs, cat# E3010L) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The pre-existing gRNA was replaced a by the newly designed DNA using the Q5 Site-Directed mutagenesis kit (New England Biolabs, M0491S), respective primers are listed in Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA libraries were generated using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (New England BioLabs, Ipswitch, MA). Resulting libraries were sequenced on a NovaSeq 6000 at a depth of 30 million reads/sample ...
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bioRxiv - Neuroscience 2022Quote: ... Three to four independently generated PCR products for each OT1-4/founder were purified using the Monarch PCR & DNA Cleanup Kit (NEB Inc.) and sent for Sanger sequencing at the OHSU Vollum Sequence Core.
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bioRxiv - Genomics 2022Quote: ... Libraries were constructed from these sheared DNA samples using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) and NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2 (NEB E7335S & E7500S) ...
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bioRxiv - Immunology 2022Quote: ... 200 ng of total RNA were used to generate barcoded RNA-seq libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs Inc). Libraries were sequenced on a HiSeq 2500 (Illumina ...
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bioRxiv - Microbiology 2022Quote: Extracted DNA was used for DNA library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the CmR cassette and the downstream region were bound together by overlap PCR and purified using Monarch DNA Gel extraction kit (New England Biolabs®).
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bioRxiv - Microbiology 2022Quote: ... Purified RNAs were used for library preparation using the NEB Next Multiplex Small RNA Library Prep kit for Illumina (E7300 L) with Universal miRNA Cloning Linker from Biolabs (S1315S) as the 3’ adaptor and in-house designed indexed primers ...
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bioRxiv - Microbiology 2022Quote: RNA-free PXO99A genomic DNAs (0.2 μg) were used to construct the DNA libraries using a NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and DHX38-FKBPF36V-2xHA_P2A_BFP were synthesized with 800bp dsDNA homology arms by Genewiz and assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB E5520S).
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bioRxiv - Plant Biology 2022Quote: ... The cassette was assembled to the backbone of CRISPR/Cas9 construct using Gibson Assembly® Cloning Kit (New England Biolabs Inc). The gRNAs targetingKTI1 and KTI3 were synthesized in one cassette at GenScript Biotech Corp ...
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bioRxiv - Plant Biology 2022Quote: RNA libraries were constructed using ribosomal-depleted RNAs as per the protocol of Ultra Directional RNA Library Prep Kit for Illumina (NEBNext, NEB, USA). The random hexamer primer plus RNaseH-(M-MuLV Reverse Transcriptase ...
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bioRxiv - Plant Biology 2022Quote: ... The RNA-Seq paired end sequencing libraries were prepared from the quantity and quality check (QC) passed RNA samples using NEBNext® UltraTMII directional RNA Library Prep Kit for Illumina (NEB) as per manufacturer’s instruction ...