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Citations for New England Biolabs :
901 - 950 of 2865 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Single or multiple amino acid mutations in full-length GPR161 were generated using Q5 site-directed mutagenesis kit (NEB).
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bioRxiv - Biophysics 2023Quote: ... Single-amino acid exchanges were generated by Q5® site-directed mutagenesis (New England Biolabs GmbH, Frankfurt am Main) using the appropriate primer pairs which were designed by using NEBaseChanger ...
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bioRxiv - Microbiology 2023Quote: 20 ng of nucleic acid was enriched for viral genomic sequences using NEBNext Microbiome DNA Enrichment Kit (NEB, E2612) to reduce host genomic DNA contamination ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was purified from single-humanized Hsα1 and quintuple-humanized Hsα1,α2,α3,α4,α7 strains using the Monarch Nucleic acid purification kit according to the manufacturer’s protocol (NEB). Spheroplasts were obtained before the genomic DNA extraction for a high-quality DNA prep ...
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bioRxiv - Biochemistry 2023Quote: ... The extracted plasmid DNA was reconstituted in cacodylic acid (10 mM, pH 6.5) and subjected to linearization by HINDIII-HF restriction digestion (New England Biolabs). Following digestion ...
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bioRxiv - Microbiology 2024Quote: ... purified nucleic acids were converted to complementary DNA (cDNA) while random primer mix (New England Biolabs, Ipswich, MA, USA) was used to prepare double-stranded cDNA using NEBNext Non-Directional RNA Second Strand Synthesis module ...
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bioRxiv - Genetics 2023Quote: ... We added phasing nucleotides as well as overhangs for indexing primers using primer mixtures SL5.F[1-4] and SL5.R[1-4] (NEB Q5 for 20 cycles, Tm 62 °C). We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles ...
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bioRxiv - Cell Biology 2020Quote: ... 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs), and 5 µl 10 mM MnCl2 for 30 min at 30 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μl 400 U/μl T4 DNA Ligase (NEB, M0202L) and 969 μl water ...
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bioRxiv - Genomics 2022Quote: Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB ...
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bioRxiv - Genomics 2020Quote: ... 5′ adapter ligation with T4 RNA Ligase 1 (NEB, M0204L). The resulting RNA fragments were used for a reverse transcription reaction using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... After a treatment with antarctic phosphatase (5 Units, NEB Inc.) for 30 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Adenylation was performed with 3’-5’ Klenow Fragment (NEB M0212L). Adaptors were ligated with NEB Quick Ligase for 10 minutes at 30°C before two rounds of cleanup with homemade beads ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μL of 5X Quick Ligation Reaction buffer (NEB: B6058S), 6 μL of the RMX adapter and 3 μL of T4 DNA ligase (2,000,000 units/mL) ...
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bioRxiv - Genetics 2020Quote: ... 5 μl of Klenow exo- (NEB M0212S/L, 5U/μl) was added ...
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bioRxiv - Genetics 2020Quote: ... and 5 μl of 400 U/μl T4 ligase (NEB). To maximize the ligation efficiency for the low input Tri-HiC protocol ...
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bioRxiv - Plant Biology 2021Quote: ... using a Klenow fragment (3′ → 5′ exo−) (New England Biolabs). Hybridization was performed at 65°C overnight in the pre-hybridization solution containing 6x saline-sodium citrate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 125 to 75 U/ 5 μg RNase B (Cat.P7817S, NEB) and fetuin (Cat ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μl of 2X RNA loading dye (New England Biolabs) was added ...
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bioRxiv - Systems Biology 2021Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB) and grown in liquid culture ...
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bioRxiv - Systems Biology 2021Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB) and grown in liquid culture.
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bioRxiv - Microbiology 2021Quote: ... or 5 µl anti-H3K18cr antibody (PTM-517, PTM Biolabs) together with protein A agarose (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... and 15 units of Klenow Fragment (3’→5’ exo-, NEB). Following extension reactions ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.25 μl of 5’Ligation Enzyme Mix (NEB-kit) followed by a 1 h incubation at 25°C ...
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bioRxiv - Cell Biology 2021Quote: ... Then 2-5 unites of RNase H (New England Biolabs) in 5 µl of 1x RNase H buffer were added to the annealed RNA-chimeric oligo mixture and the RNase H cleavage reaction was performed at 37o C for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... Toxicity-resistant strains such as NEB 5-alpha F’Iq (NEB) and ABLE K (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA products were successively treated with 5’ pyrophosphohydrolase (NEB) and polynucleotide kinase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’ linkers harboring barcodes sequences and EcoP15I (NEB, cat. R0646S) recognition site were ligated ...
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bioRxiv - Biochemistry 2022Quote: ... A-tailing (Klenow fragment (3’-5’ exo–, New England Biolabs), ligation to barcoded adapters (KAPA) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 5 units/µl RNase H (NEB) were added and samples were incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... coli dam+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/ dcm− (dam−/dcm− competent E ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl of 400 U/µl T4 DNA Ligase (NEB), and incubated at room temperature for 4 hours.
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bioRxiv - Developmental Biology 2022Quote: ... 5’-CTTTTGACATCCGCTTCTGC-3’ followed by a T7 endonuclease assay (NEB) to detect indels ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by A-tailing (Klenow 3’-> 5’ exo-, NEB, M0212S). After A-tailing ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% DMSO and 10 units of λ exonuclease (NEB, M0262S) at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... but with 5 units of restriction enzyme Xho I (NEB) mixed with 750ng DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 units/ml proteinase K (New England Biolabs P8107S) at 55°C for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... We added 5 uL 1X T4 Ligase Buffer (NEB # B0202S), 0.5 uL T4 PNK (NEB #M0201S) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 10 μl of 5 U/μl Klenow (NEB M0210)) and incubating 1h at 37ºC with rotation ...
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bioRxiv - Microbiology 2022Quote: ... a custom 3’ adapter (5’-rAppCTGTAGGCACCATCAAT–NH2-3’, NEB, S1315S) was ligated to all RNAs ...
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bioRxiv - Genomics 2020Quote: ... The 5’ overhang was repaired using T4 DNA polymerase (NEB) with dNTP (200 μM ...
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bioRxiv - Genomics 2020Quote: ... and dephosphorylated by addition of 5 μl rSAP (NEB, #M0203) at 37°C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: RNAs were 5’ capped using the Vaccinia capping system (NEB). For each reaction of 20 μl ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µl of Taq 5× Master Mix (New England Biolabs) and Milli-Q water ...
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bioRxiv - Microbiology 2020Quote: ... and 5 mM sodium fluoride (New England Biolabs, Ipswich, MA) and 1 mM sodium orthovanadate (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: 5’ phosphorylation of micro HHrz RNA with polynucleotide kinase (NEB) as done following manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by digestion with 5 U RNase H (NEB, M02975) for 30 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Clarified supernatants were digested with 5 µL MNase (NEB M0247S) and incubated for 30 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Unligated linkers were depleted with yeast 5’-deadenylase (NEB #M0331S) and RecJ exonuclease (Epicentre #RJ411250 ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... 5% v/v PNGaseF (New England Biolabs, Ipswich, MA, USA) and 50 mM NEM were added to lysates mixed with 1 × GlycoBuffer and incubated on ice for 1 h ...