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Citations for New England Biolabs :
901 - 950 of 5823 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... followed by PCR amplification with Phusion DNA polymerase (NEB) for 15 cycles ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were digested using DpnI (NEB, Ipswich, MA) and purified using the MicroElute Cycle Pure kit (Omega ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by PCR amplification with Phusion DNA polymerase (NEB) for 15 cycles ...
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bioRxiv - Developmental Biology 2024Quote: ... and NEBNext High-Fidelity 2X PCR Master Mix (NEB). Samples were sequenced with Illumina Hi-Seq technology according to standard protocols.
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bioRxiv - Microbiology 2024Quote: ... Luna One-step qRT-PCR kit (New England Biolabs) was used with 1 μL supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... The solution was diluted by adding 7 ml of ligation buffer (NEB) containing 1% Triton X-100 and 30 Units of T4 DNA ligase (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... 26ul 20x SSC solution (InvitrogenTM 15557044) and 15ul RNase inhibitor (NEB M0314L). The 2ml tube was put in 50 °C water bath overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... These targets were precipitated out of solution with streptavidin magnetic beads (NEB) and the DNA was isolated out of the complexes with Proteinase K (Amresco ...
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bioRxiv - Genomics 2021Quote: ... 0.5 μL of 10 mM Deoxynucleotide (dNTP) solution mix (New England Biolabs) was added to each reaction and nuclease -free water was used to make up the remaining reaction volume ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was assembled with the following: 3.2 µL solution A (NEB), 1 µL factor mix (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting aqueous solution was concentrated using a spin column (Monarch, NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... dsDNA was digested by MmeI solution (1× NEB Cutsmart, 2 U MmeI) at 37 °C for 0.5 h and extracted with 12% native polyacrylamide TBE gel (∼84 bp band) ...
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bioRxiv - Biochemistry 2022Quote: ... The working solution at 1.53 μM was obtained using Diluent A (NEB) and 1% Triton X-100 ...
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bioRxiv - Genomics 2023Quote: ... TET2 reaction was terminated by addition of 0.6 μL stop solution (NEB) and incubation at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by in-solution DNase I treatment (8 Units, New England Biolabs) according to manufacturer’s instructions for 30 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.5 mg DNase I solution (New England Biolabs, Ipswich MA, USA) in PBS and incubated at 37 °C and 5 % CO2 for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
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bioRxiv - Plant Biology 2021Quote: The first round of PCR was performed using 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs; Ipswitch, MA, USA) with forward primer “reverse transcription primer (RTP)” ...
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bioRxiv - Plant Biology 2021Quote: The first round of PCR was performed using 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs; Ipswitch, MA, USA) with cDNA diluted 1:10 in water ...
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bioRxiv - Zoology 2020Quote: ... of the reaction was then checked for successful amplification on a 1% agarose with the 1 kb DNA Ladder from New England Biolabs and afterwards the remaining PCR product was purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ipswich, MA). Purified products for S ...
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bioRxiv - Cancer Biology 2022Quote: pcDNA3-TNFR1 expression vector was generated by cloning with the primers indicated below to PCR amplify (using Q5 High-Fidelity PCR kit, New England Biolabs, Euroclone, Milan, Italy) the TNFR1 reference sequence from pBMNZ-neo-Flag-TNFR1 L380A (gift from Martin Kluger ...
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bioRxiv - Systems Biology 2023Quote: ... PCR with custom Nextera PCR primers was then performed for five cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs, Ipswich, MA, USA) 76 ...
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bioRxiv - Microbiology 2023Quote: ... Amplification was achieved through 16S rRNA gene Polymerase Chain Reaction (PCR) with Illumina (San Diego, USA) adapted primers [50] and NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich, USA) at 98°C (2 minutes ...
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bioRxiv - Genomics 2023Quote: ... Amplification of the libraries was performed for 13 PCR cycles using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, cat. no. M0531L); 6-bp molecular barcodes were also incorporated during this PCR amplification ...
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bioRxiv - Genetics 2021Quote: ... The entire alh-6 genomic sequence (ATG to stop) was amplified by PCR and cloned in a linearized pMiniT 2.0 vector (NEB PCR Cloning Kit, Cat. #E1202S). Plasmid DNA was purified using the Zymo Research Zyppy Plasmid Miniprep kit (Cat ...
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bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
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bioRxiv - Cell Biology 2022Quote: ... The V3-V4 hypervariable regions of bacterial 16S rRNA genes were amplified by PCR with Phusion® High-Fidelity PCR MasterMix (New England Biolabs, Ipswich, MA, USA) using specific primer as previously described59 ...
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bioRxiv - Genetics 2022Quote: ... DNA samples were purified before and after PCR with the Monarch® PCR and DNA Clean-Up Kit (New England Biolabs, Ipswitch, MA, USA). Individual sample libraries were pooled ...
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bioRxiv - Genomics 2021Quote: ... and 7 PCR cycles were used to amplified libraries using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich MA, Catalog #M0541S) and SYBR Green I (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... 5’-GCT GCG TTC TTC ATC GAT GC-3’) were chosen with barcodes for PCR amplification using Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA). The PCR products were then mixed at equal density ratios and purified with a Gel extraction kit (QIAGEN ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid was directly amplified using Q5 polymerase using Polymerase chain reaction (PCR) (Q5® High-Fidelity PCR Kit, New England Biolabs, Ipswich, MA, USA). PCR was carried out following manufacturer protocol (Table S3 ...
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bioRxiv - Genomics 2020Quote: ... 2 μL 10 mM dGTP and 10 mM dTTP (stock solutions: NEB, N0446), 10 μL 10x T4 DNA Ligase Reaction Buffer (NEB B0202S) ...
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bioRxiv - Bioengineering 2021Quote: ... the plasmid solutions were incubated for Nb.Bsmi nicking enzyme (R0706S, New England Biolabs) for 60 minutes at 65°C in NEBuffer 3.1 ...
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bioRxiv - Genomics 2020Quote: ... 2 μl 10 mM dGTP and 10 mM dTTP (stock solutions: NEB, #N0446), 10 μl 10x T4 DNA Ligase Reaction Buffer (NEB #B0202S) ...
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bioRxiv - Biophysics 2021Quote: ... 200μl of a 50ng/μl solution (using Lambda DNA, New England Biolabs (NEB) N3011S) ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were quenched on ice and by addition of 1x Stop Solution (NEB). Immediate isopropanol-based precipitation recovered 75- 95% of input mRNA as mRNA fragments in water.
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bioRxiv - Neuroscience 2022Quote: RNA solution was then treated with DNase I (RNase-free) (New England Biolabs) to remove genomic DNA from sample and protected against RNA degradation using RNasin® Ribonuclease Inhibitor (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 μl of deoxynucleotide (dNTP) solution mix (40 μM; New England Biolabs, UK), 1 μl of forward primer (10 μM ...
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bioRxiv - Biophysics 2023Quote: ... The solution was supplemented with 0.1 vol of 10X T4 ligase buffer (NEB) and T4 ligase (30 U.μL-1 ...
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bioRxiv - Microbiology 2024Quote: ... the chamber was blocked with 1 mg/ml bovine serum albumin solution (NEB) for 5 min ...
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bioRxiv - Genomics 2024Quote: ... a 750 μl solution containing 1x NEB T4 DNA ligase buffer (NEB B0202), 120 μg of BSA (ThermoFisher AM2616) ...
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bioRxiv - Microbiology 2024Quote: ... the chamber was blocked with 1 mg/ml bovine serum albumin solution (NEB) for 5 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... All plasmid-template PCR products were treated with DpnI (NEB) following standard protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR amplified (8-12 cycles) with Phusion polymerase (NEB) using custom primers (22) ...
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bioRxiv - Genetics 2020Quote: ... We performed this PCR using Taq DNA Polymerase (M0320S, NEB) and the following conditions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (NEB). Each reaction contained 1x Q5 reaction buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1X Phusion High Fidelity PCR Mastermix (New England Biolabs). Cycle conditions were 98°C for 30 seconds ...
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bioRxiv - Immunology 2022Quote: ... and Luna® Universal RT-PCR Kit (New England Biolabs) in a StepOnePlus apparatus (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA polymerase (NEB) while qPCR products were amplified using Ssofast EvaGreen Supermix (BioRad ...