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Citations for New England Biolabs :
901 - 950 of 2080 citations for 2 6 Amino 9H purin 9 yl ethanol d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and cloning was performed using the Gibson Assembly 2× Master Mix (NEB) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and once with 2 bead volumes of 1X RNase H Buffer (NEB; 50mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: ... using 2 μl of NEBNext dsDNA Fragmentase (New England Biolabs, cat. M0348), supplemented with 1 μl 200 mM MgCl2 ...
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bioRxiv - Bioengineering 2020Quote: ... the SARS-CoV-2 RNA was obtained by in vitro transcription (NEB, HiScribe™ T7 High Yield RNA Synthesis Kit ...
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bioRxiv - Developmental Biology 2021Quote: The DNA was then digested with 2 μL CutSmart Buffer (NEB, R0137L) and 1 μL Alul enzyme (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was rocked with 2 mL of amylose resin slurry (NEB) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... To release N-glycans 2 μl of PNGase F (New England Biolabs) was added and the samples were incubated for 18 h in 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Linker-2 (5′-GAGTCTGCGTGTGATTCGGGTTAGGTGTTGGGTTGGGCCA-3′) was ligated using T4 RNA Ligase1 (NEB). Reaction mixtures were resolved on a 10% TBE-Urea gel ...
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bioRxiv - Microbiology 2021Quote: ... mixed with equal volume of 2× RNA Loading Dye (New England Biolabs), heated at 90 °C for 2 min ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 2 µL of 20 mg/mL RNaseA (New England BioLabs) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µM IllLigBio adapter pre-adenylated using a 5’ adenylation kit (NEB), 20-200 nM pppUGAAUG hexamer standard ...
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bioRxiv - Microbiology 2022Quote: ... 2 μL of NEBNext FFPE DNA Repair Mix (New England Biolabs, UK), 3.5 μL Ultra II End-prep reaction buffer ...
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bioRxiv - Microbiology 2023Quote: ... 2 μg 5’PPP transcripts were incubated with 10 U RppH (NEB) and 20 U RiboLock (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 2 µL BsaI-HF v2 Golden Gate enzyme mixture (New England Biolabs), 4 µL 10× T4 Ligase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 units/µl of murine RNase inhibitor (New England Biolabs, M0314L). In minigene experiments ...
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bioRxiv - Developmental Biology 2023Quote: ... Digestion was performed with 2 µl (10U) of thermostable RNaseH (NEB #M0523S) and 2 µl of 10X buffer in a 20 µl volume at 65° C for 5 min ...
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bioRxiv - Genomics 2023Quote: ... and 2 µl CutSmart buffer (New England Biolabs, cat. no. B7204S, 10×) at 37°C for 2h ...
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bioRxiv - Genomics 2023Quote: ... dsDNA was digested by MmeI solution (1× NEB Cutsmart, 2 U MmeI) at 37 °C for 0.5 h and extracted with 12% native polyacrylamide TBE gel (∼84 bp band) ...
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bioRxiv - Genetics 2022Quote: ... were ligated using a 1:2 mixture of T4 ligase (NEB, M0202L) and T4 polynucleotide kinase (NEB ...
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bioRxiv - Genomics 2022Quote: ... 1X NEB Buffer 2 and 0.20μg/μl BSA (all from NEB, USA) was added to the wells and incubated at 25°C for 1.5h ...
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bioRxiv - Bioengineering 2024Quote: ... A mastermix containing 2 mmol/L of each dNTP (NEB, Ipswich, MA), 4.5 µg of random hexamers (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... 2 µl of 100µM DTT and 1 µl of NudC (M0607S, NEB), then incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 DNA fragment plasmids were digested with I-SceI (NEB) to release the overlapping fragments from the backbones ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37 °C for 2 hours followed by digestion with PspXI (NEB) in rCutsmart™ Buffer (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... 2 and 3 were assembled by PCR assembly using Vent polymerase (NEB), with each reaction consisting of 1 x ThermoPol buffer ...
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101 ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL of a 0.4 µg µL-1 stock of Trypsin (NEB) were added to the samples (to a final enzyme:substrate ratio of 1:50 w/w ...
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bioRxiv - Biochemistry 2023Quote: ... 15% PEG8000 and 20 U T4 RNA ligase 2 truncated KQ (NEB)) ...
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bioRxiv - Biochemistry 2023Quote: ... of the SARS-CoV-2 Positive Control (N gene) plasmid (NEB N2117). Sanger sequencing confirmed successful mutagenesis ...
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bioRxiv - Genetics 2023Quote: ... the probe was digested by 2 units of DNaseI (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Genetics 2023Quote: ... 2 μL 1 M CaCl2 and 5 μL micrococcal nuclease (NEB, #M0247S) were added and chromatin was fragmented into predominantly mono-nucleosomes by incubation at 37°C for 15 min ...
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bioRxiv - Genetics 2023Quote: ... 2 μL RNase inhibitor (M0314; New England Biolabs Inc.; Ipswich MA, USA) and 2 μL MNase (M0247 ...
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bioRxiv - Genetics 2023Quote: ... and 2 μL MNase (M0247; New England Biolabs Inc.; Ipswich MA, USA) were mixed in and tubes promptly placed on ice ...
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bioRxiv - Microbiology 2023Quote: ... for 30 min and 2 μl Proteinase K (New England BioLabs, P8107S) for 1 h ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... The diluted sample was treated with 2 µl Lambda protein phosphatase (NEB) and incubated in water bath at 30 ℃ for 4 hrs ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 mM dCTP and 2 U of terminal transferase (New England Biolabs). The mix was incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated with 2 µM SNAP-Cell Oregon Green (NEB) for 20 minutes at 37°C to label newly synthesized RPL23a ...
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bioRxiv - Microbiology 2024Quote: ... The RNE-Apex2FlgC-EGFPC-2 plasmid was assembled via Gibson assembly (NEB) and transformed into chemically competent DHbeta10 E ...
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bioRxiv - Microbiology 2024Quote: ... 20 µl Q5® High-Fidelity DNA Polymerase (2 U/µl, NEB), 160 µl Taq DNA ligase (40 U/µl ...
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bioRxiv - Microbiology 2024Quote: ... cenocepacia K56-2 genome using Q5 polymerase with high GC buffer (NEB) and primers 3107 and 3108 (Supplementary Table 10 ...
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bioRxiv - Biochemistry 2024Quote: ... with or without 2 µL Lambda protein phosphatase (NEB, 400000 U/mL). Reactions were carried out for various times ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2 to 3 μg plasmid was digested with 1 μl NotI (NEB) for 1 h at 37 °C and heat inactivated prior to transformation ...
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bioRxiv - Genetics 2021Quote: ... The entire alh-6 genomic sequence (ATG to stop) was amplified by PCR and cloned in a linearized pMiniT 2.0 vector (NEB PCR Cloning Kit, Cat. #E1202S). Plasmid DNA was purified using the Zymo Research Zyppy Plasmid Miniprep kit (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 10x T4 ligase buffer, 3 µL 50 mg/mL BSA, 1.5 µL 2000U/µL T4 ligase, NEB, 6 µL BLISS adapter pairs). For removal of excess adapters ...
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bioRxiv - Genomics 2024Quote: ... Samples were then rinsed with 2X SSC before incubating with a blocking buffer (10% BSA, 3% v/v 6% v/v murine RNase inhibitor [NEB, M0314L] in 2X SSC) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... Equal amount of total RNA in a maximal volume of 6 µ L is used for cDNA synthesis using NEB PhotoScript® First Strand cDNA Synthesis Kit (Catalog # E6300L, NEB Inc, Ipswitch, MA) as instructed in the manufacturer standard protocol ...
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bioRxiv - Biochemistry 2024Quote: ... A His-Tag encoding sequence was added to the C-terminus of RnfG by using oligonucleotides 5 and 6 via site-directed mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB, Frankfurt am Main, Germany). The resulting plasmid was checked by sequencing analysis (Microsynth Seqlab ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg DNA spiked with methylation standards was digested with SmaI endonuclease (NEB) at 25°C for 8 h ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... Q5-high Fidelity 2× master mix was used as polymerase from NEB (# M0429L). PCR condition for the 1st PCR was ...