Labshake search
Citations for New England Biolabs :
9351 - 9400 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... leprae positive faecal and necropsy samples (Supplementary Information Table 1) were converted into dual-indexed libraries using the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs, MA, USA)57,58 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA fragments were converted to library using NEBNext® Small RNA Library kit (ref#E7330S, New England Biolabs, UK or equivalent from Illumina, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 3 μg RNAs for each sample were used for library construction using NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA, cat. no. E7530) according to the instruction and RNA-Seq on Illumina Hiseq 2500 platform.
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bioRxiv - Developmental Biology 2020Quote: ... digested pCFD6 backbone to generate UAS-t::gRNA-twi4x using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, Cat. No: R5520S), following the instructions provided by the manufacturers.
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... The DAP gDNA library was prepared using the kit from NEBNext® DNA Library Prep Master Mix Set for Illumina® (NEB #E6040S/L). ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A ...
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bioRxiv - Cell Biology 2020Quote: ... and the resulting derivatives were linearized and used as templates for in vitro RNA transcription with the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA); 1.5 μg template was incubated for 4 hrs following kit protocols ...
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bioRxiv - Genomics 2021Quote: Whole Genome DNA libraries with average insert size of 200 bp were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, 2016) using the protocol recommended by the company ...
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bioRxiv - Plant Biology 2021Quote: ... cablin seedlings and were introduced into the expression vector pET-28a (+) between the restriction sites of NdeI and BamHI using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, Catalog number: E5520S), in each case generating a fusion gene that encoded a “tag” of HIS6 residues at the N-terminus for expression in E ...
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bioRxiv - Microbiology 2020Quote: ... samples were treated using a Pan-Prokaryote riboPOOL™ kit (siTOOLs Biotech Gmbh, Planegg, Germany) coupled with streptavidin magnetic beads (New England Biolabs, Ipswich, MA, USA). mRNA was eluted in 50 μl of nuclease-free water and then cleaned and concentrated using a Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... Three recB fragments were directly assembled into pET28c vector in frame with the N-terminal 6xHis tag employing NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Inc., Ipswich, MA, USA). Wild type RecC encoding gene was individually sub-cloned into pET28c vector in frame with the N-terminal 6xHis tag ...
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bioRxiv - Genomics 2021Quote: ... cDNA libraries were synthesized using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (Both: New England Biolabs, Ipswich, Massachusetts) and subsequently sequenced in a paired-end mode with 75bp read length on NextSeq500 (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg gDNA was then used to prepare the gDNA sample library using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and sequencing libraries were constructed using a NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, E7420L) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Frankfurt am Main, Germany) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... 200 ng of DNA was used for transcription by T7 polymerase (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB, Ipswich, MA, USA). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... We created DNA libraries with an average library size of 320 bp using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Library preparation and sequencing were prepared with Illumina-compatible NEBNext® Ultra™ II using Directional RNA Library Prep Kit (New England BioLabs, MA, USA). Viral total RNA was taken for fragmentation and priming ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We used a standard miniaturized protocol to prepare DNA libraries using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. (Ipswich, MA, USA))56 ...
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RTEL-1 and DNA polymerase theta promote subtelomeric DNA synthesis and telomere fusion in C. elegansbioRxiv - Genetics 2022Quote: ... and pELS53 [Peft-3::Cas9 + rtel-1 sgRNA] plasmids were generated for this study by modifying the pDD162 plasmid (Dickinson, 2013) using Q5 Site Directed Mutagenesis Kit (NEB, Cat# E0554S, Ipswich, MA).
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bioRxiv - Microbiology 2022Quote: ... with duplicate sample in each group) was depleted of bacterial rRNA using NEBNext® rRNA depletion kit (Cat. no. E7850X, New England Biolabs, Ipswich, MA,USA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... and reversely transcribed into cDNA from 1 μg of mRNA template using the M-MuLV cDNA Synthesis Kit (New England Biolabs, Frankfurt am Main, Germany) according to the protocol of the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were generated from 1 μg RNA per sample using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Whole mRNAseq libraries were generated using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). The clustering of index-coded samples was then performed on a cBot Cluster Generation System ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were generated from 1 µg total RNA per sample using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... dsRNA was synthesized from T7-linked DNA using the HI Scribe™ T7 High Yield RNA Synthesis Kit (New England, Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... strand-specific total-transcriptome RNA sequencing libraries were prepared by incorporating dUTPs in the second-strand synthesis using NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA). The isolated cell RNA-seq libraries were prepared using the Ovation® SoLo® RNA-Seq library preparation kit (Tecan ...
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bioRxiv - Developmental Biology 2022Quote: The RNA-seq libraries were prepared from 100 μg of total RNA per genotype with NEBNext Ultra II RNA Library Prep Kit mRNA Isolation Module (NEB, Cat# E7775S, E7490S, E7500S). Quality control and sequencing of the RNA-seq libraries were performed by GENEWIZ ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared from the enriched mRNAs using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA). The library concentration was determined in a Qubit3 Fluorometer using the Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Molecular Biology 2023Quote: ... These immuno-enriched DNA samples could be used for NGS after DNA library construction with NEBNext® UltraTM II DNA Library Prep Kit (NEB, USA, Catalog #: E7645L). The qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy in Novogene Bioinformatics Technology Co. ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA-seq libraries were constructed using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Finally ...
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bioRxiv - Evolutionary Biology 2024Quote: ... For Psav we used a standard miniaturized protocol to prepare DNA libraries for sequencing using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. (Ipswich, MA, USA)) ...
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bioRxiv - Zoology 2024Quote: ... Purified dsRNA templates were reamplified from the L4440 vector with T7 promoter oligonucleotide sequences and used for dsRNA synthesis by in vitro transcription using HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs, Whitby, ON, Canada) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from agar plate-grown seedlings by acid guanidinium thiocyanate-phenol-chloroform-based extraction and purified from the aqueous phase using the Monarch RNA Clean Up Kit (New England Biolabs, Ipswich, MA, USA; NEB). Genomic DNA in the samples was removed using TURBO™ DNase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Strand-specific libraries were generated from the rRNA-depleted RNA using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: ... and the processed RNA samples were used to construct RNA-seq libraries using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA). Then the RNA libraries were sequenced using the Illumina HiSeq 4000 platform to generate 150bp paired-end reads ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we used PCR with long protruding primers to add GGS sequences and BsaI sites directly to the genes of mTurquiose2 and mVenus and assembled them with the pET09 via NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2) (NEB, Ipswich, MA). Control proteins were PCR amplified from either E.coli DH5 αgenome or in-house generated constructs ...
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bioRxiv - Cancer Biology 2023Quote: ... The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs, NEB) protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Benzonase was inactivated using 50 mM EDTA and DNA was then extracted using Monarch Genomic DNA purification kit (New England Biolabs, Ipswich, MA, USA, #T3010S).
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bioRxiv - Neuroscience 2022Quote: ... cDNA libraries were prepared the same day using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB E7760). All samples were amplified using 8 cycles of PCR.
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bioRxiv - Plant Biology 2023Quote: ... and then the sequencing library was constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Japan Inc., Tokyo, Japan). The library was sequenced using the illumina NovaSeq 6000 platform with S4 flow cells to produce 150 bp paired-end and dual index reads ...
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bioRxiv - Cancer Biology 2022Quote: ... the fragments were purified and used for adapter ligation and library amplification using the NEBNext Ultra II Fs DNA Library kit for Illumina (NEB # E7805, New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... UAS expression constructs were generated by combining the linearized pGW-HA.attB backbone and each individual ZIKV fragment in Gibson assembly reactions (NEB Gibson Assembly Cloning Kit, E5510S). To generate NS4A::2K ...
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bioRxiv - Microbiology 2023Quote: ... and the expression levels of genes were quantified with the Luna® universal one-step RT-qPCR kit (New England Biolabs, Ipswich, MA, USA) with the following cycling parameters ...
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bioRxiv - Microbiology 2023Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (New England BioLabs, Frankfurt am Main, Germany). Sequencing was performed by using the HiSeq2500 instrument (Illumina Inc. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... where a cDNA library was prepared with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) and 150 paired-end transcriptome sequencing was conducted using an Illumina NovaSeq6000 sequencer (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... Whole genome enzymatic methyl-seq libraries were prepared using the NEBNext® Enzymatic Methyl seq kit (New England BioLabs® Inc., Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were further lysed with probe sonication and the lysate was denatured by adding 10 µL of denaturing buffer (NEB PNGase F kit, P0709) and heating at 100 °C for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, Cat. No. E7490L, E7760L) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...