Labshake search
Citations for New England Biolabs :
9051 - 9100 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... promoters were inserted into the pH7WG Gateway vector (Department of Plant Systems Biology, Ghent University, Belgium (Karimi et al., 2002)) by the Gibson Assembly kit (New England Biolabs, Ipswich, MA, USA). The tagged PIN7 and PIN4 cDNA entry clones were then recombined with the modified pH7WG destination vector by the Gateway LR reaction (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... and used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), following manufacturer instructions.
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bioRxiv - Cell Biology 2021Quote: ... Amplicons and pCineo plasmid (linearized by NheI/EcoRI digestion) were assembled using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs, cat. no. E5520), following the manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: Extracted viral RNA was reverse transcribed and tagged with index adaptors using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... In total 27 libraries (3 species x 9 time points) were prepared according to NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, U.S.A.) followed by paired end sequencing (100bp x 2 ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 1 μg RNA was used as input material for library generation using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB USA), at which point index codes were added to each sample ...
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bioRxiv - Genetics 2021Quote: ... sgRNAs were synthesized directly from gBlock® DNA (IDT) templates containing the T7 promoter using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England BioLabs E2050) following manufacturer’s instructions for sgRNA synthesis.
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bioRxiv - Microbiology 2021Quote: ... The resulting sheared DNA was used for library preparation using the NEBNext Ultra II Library preparation kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The amplified mRNA of each sample was used to construct sequencing libraries using NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, USA) according to the instruction manual ...
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bioRxiv - Plant Biology 2021Quote: ... a non-adenylated 3’ adapter was ordered for chemical synthesis from IDT™ and adenylated with the 5’ DNA Adenylation Kit (New England Biolabs® Inc.) (see Supplemental Table 4 for primers and adapters used) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-Seq libraries were generated from 2 µg RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7490 and #E7420). Libraries were quantified using the Agilent DNA 7500 Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed to enrich for sub-nucleosomal fragments using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina as described (NEB, E7645S). The libraries were size-selected and purified with AMPure XP beads ...
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bioRxiv - Cell Biology 2021Quote: pcDNA4/TO-GFP-CLC7-Y715C mutation was generated by site-directed mutagenesis of the pcDNA4/TO-GFP-CLC7 expression vector using the Q5™ Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) and complementary primer pairs (forward ...
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bioRxiv - Biochemistry 2022Quote: Cysteine mutations were introduced into a pENTR1A FLAG (FT) WT F3 or pENTR1A 3xFT WT F3 plasmid using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, USA). pENTR1A constructs were shuttled into the pcDNA DEST40 vector (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... Quantification of enriched sequencing libraries was performed using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs, Ipswich, Massachussets, USA). Libraries were then pooled and sequenced on an Illumina Miseq System (Illumina ...
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bioRxiv - Physiology 2022Quote: ... in zebrafish f5 proximal promoter sequences (Fig. 4E, Supplemental Table 5) (28) was conducted using a Q5 site-directed mutagenesis kit (NEB, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: In vitro transcription/translation by codon skipping of the short FLAG tag-containing peptides X-Val-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (XV-Flag) where X = 7, 13, 14, or 15 was carried out using the PURExpress® Δ (aa, tRNA) Kit (New England Biolabs, E6840S) based on a previous protocol with slight modifications 16 ...
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bioRxiv - Developmental Biology 2022Quote: ... and 500 ng of RNA was used for cDNA library construction with NEBNext ultra II directional RNA library prep kit for Illumina as recommended by the manufacturer (New England Biolabs, Inc, Ipswich, MA). cDNA libraries were validated on the TapeStation (Agilent ...
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bioRxiv - Genetics 2022Quote: ... We inserted TFT reporters into BFA0190 by digesting the plasmid with SwaI and inserting TFT components between the LYP1 flanking sequences using isothermal assembly cloning (Hifi Assembly Cloning Kit; New England Biolabs [NEB], Ipswich, MA, USA). The 5’ and 3’ LYP1 flanking sequences in each TFT plasmid contain natural SacI and BglII restriction sites ...
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bioRxiv - Plant Biology 2022Quote: ... and pCambia1305.1 vectors were prepared and subsequently digested using NcoI restriction endonuclease prior to purification using Monarch DNA & PCR Cleanup Kits (New England Biolabs, Ipswich, MA, USA). Vector and insert concentrations were estimated using a Synergy H1 microplate reader (BioTek Instruments ...
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bioRxiv - Microbiology 2022Quote: ... Five microliters of resulting clean RNA was used for first- and second-strand cDNA synthesis and library preparation using NEB Ultra II Directional RNA library prep kit for Illumina (New England Biolabs, Ipswich, MA, USA). Libraries were barcoded with unique dual indices synthesized in the CDC Biotechnology Core Facility Oligonucleotide Synthesis Laboratory ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted from organoids (three biological replicates for each group) by TRIzol and the rRNAs were removed with NEBNext rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA). RNA libraries were constructed using the NEBNext® Ultra(tm ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg RNA was used to generate a pair-end sequencing library using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to track sequences to each sample ...
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bioRxiv - Microbiology 2020Quote: ... and further ligated with illumina adaptor sequences using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs). The prepared libraries were purified using AMPure XP beads and sequenced as 2 × 250 bp on MiSeq Reagent Kit v2 (illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... GAL4-Creb5(1-128)T59/T61A and GAL4-Creb5(1-128)C18/C23S were generated by inducing point mutations in the GAL4-Creb5(1-128) vector using the Q5® Site-Directed Mutagenesis Kit (NEB, Cat#: E0554S). The control vector GAL4(1-147 ...
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bioRxiv - Molecular Biology 2021Quote: Variants of concern (VOC) of SARS-CoV-2 were created with Q5® Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions ...
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bioRxiv - Genomics 2020Quote: ... 3 μg of RNA from each species was used to prepare sequencing libraries using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Hi-C library was prepared on beads using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina and NEBNext® Multiplex Oligos for Illumina (New England Biolabs) following instructions from the Arima Hi-C kit ...
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bioRxiv - Molecular Biology 2020Quote: A construct for the deletion of a 9,478 bp region containing genes putatively involved in arsenic resistance was similarly constructed using the NEBuilder assembly kit (New England Biolabs, Ispwich, MA, USA). Genomic DNA on the 5’ flank of the region was amplified using primers AP34 and AP35 ...
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bioRxiv - Immunology 2021Quote: ... and converted to cDNA with ProtoScript® II First Strand cDNA Synthesis Kit (cat. n° E6560L, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturing specifications ...
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bioRxiv - Immunology 2021Quote: ... and converted to cDNA with ProtoScript® II First Strand cDNA Synthesis Kit (cat. n° E6560L, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturing specifications ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were prepared using either the “NEBNext Ultra II DNA Library Prep Kit for Illumina®” (for old herbarium specimens) or the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina®” (for more recent specimens; New England BioLabs, Ipswich, USA). In both cases ...
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bioRxiv - Microbiology 2020Quote: ... amplified with the primers Dhm1275-1 and −2 (Table 2) using a NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids expressing cysteine substitution mutant derivatives of pMTD372 and pMTD607 were generated using the Q5 Site-Directed Mutagenesis Kit (NEB catalog number E0554S).
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bioRxiv - Cancer Biology 2019Quote: ... Libraries for whole genome sequencing were prepared with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs, Inc., Ipswich, MA). Libraries were sequenced on a NovaSeq S1 flow cell (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the eluted RNA was input to the library prep using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA).
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bioRxiv - Cancer Biology 2022Quote: ... and bar-coded libraries were constructed using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and sequenced single-end (1×75 ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic localization of BioTAP-Pcl and -Jarid was determined by high-throughput DNA sequencing of libraries generated from BioTAP-XL purified genomic DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, catalog no. E7645) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... The sheared DNA was prepared for sequencing library preparation with the NEBNext Ultra II Library preparation kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Strand-specific cDNA libraries were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Sequencing was performed using NovaSeq6000 (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng of RNA was used to create indexed cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs, Ipswich, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Products were visualized on 2% agarose gels and successful amplification reactions were prepared for Sanger sequencing with Exo-CIP™ Rapid PCR Cleanup Kit according to the manufacturer’s protocol (New England Biolabs: E1050S, Ipswich, MA). Samples were then sequenced with amplification primers (Eurofins Genomics Services ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were prepared using NEBNext FFPE DNA Repair Mix in combination with the NEBNext Ultra II DNA Library Prep kits for Illumina (New England Biolabs, Ipswich, MA, USA) per manufacturer recommendations with custom inline barcodes as described in Moore et al ...
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bioRxiv - Cancer Biology 2022Quote: ... The libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England BioLabs® Inc., USA). For sequencing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and used to produce RNA-seq libraries with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: All plasmids were constructed through Gibson assembly (Gibson et al., 2009) using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Ipswich, MA, USA). Primers used in this study were purchased from Eurofins Genomics (Ebersberg ...
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bioRxiv - Genomics 2019Quote: ... Short-insert of 250 bp paired-end libraries were prepared for both individuals using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (Biolabs) and 10 PCR cycles ...
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bioRxiv - Cell Biology 2019Quote: ... and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina®(NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Microbiology 2019Quote: ... One hundred ng RNA from each sample were prepared for cDNA sequencing using NebNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The reaction was topped up to 200μL with DEPC treated water and a phenol chloroform extract performed (as before except the pellet was resuspended in 17.5μL DEPC treated water. The SR RT primer (NEB small RNA kit for Illumina) was hybridized to the 3’ adapter and the 5’ adapter ligated by adding 2μL T4 RNA ligase buffer (NEB) ...