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Citations for New England Biolabs :
9201 - 9250 of 10000+ citations for Human Complement Component 1 Q Subcomponent Binding Protein HABP1 C1QBP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... The synthesized RBS-Bdh-FLAG insert was resuspended in water to a final concentration of 10 ng/µL and was assembled with linearized pBBR1-MCS2 plasmid using NEBuilder High Fidelity DNA assembly kit (New England Biolabs) using 50 ng of pBBR1-MCS2 and 100 ng of RBS-Bdh-FLAG insert ...
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bioRxiv - Biochemistry 2019Quote: ... expression constructs containing WT or mutant full-length human KAP1 preceded by a triple FLAG tag and linker sequence MDYKDHDGDYKDHDIDYKDDDDKGSGG were assembled in pLEXm (35) with the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). The following plasmids were used ...
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bioRxiv - Plant Biology 2021Quote: ... All other mutations of the constructs used for in vitro and in vivo assays were introduced using a Q5 site-directed mutagenesis kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... PCR mutagenesis to create site-directed mutants of malM 3’-UTR and cspE 3’-UTR was conducted with the Q5 site-directed mutagenesis kit (New England Biolabs) using end-to-end primers designed with NEBaseChanger ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μl cDNA reaction mix was amplified by adding 25 μl of Long Amp Taq 2x Master Mix (NEB-kit), 1.25 μl SR primer for Illumina (NEB-kit) ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing adaptors were then added on using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs #E7645S) following the manufacturer’s instructions for a 200 bp insert size distribution and using AMpure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were generated using the NEBnext small RNA library preparation kit according to instructions (E7580S, New England BioLabs).
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bioRxiv - Microbiology 2020Quote: ... All pcDNA3.1 P deletion mutants in the full-length construct were generated by using the Q5 site-directed mutagenesis kit (New England BioLabs), following the manufacturer recommendations ...
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bioRxiv - Genetics 2020Quote: ... The RNA was then diluted 1:50 and 2 mL were used to perform a one-step qPCR protocol using Luna Universal One-step qPCR kit (NEB). Two primer sets were used ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: The libraries were generated by using the NEBNext ultraII DNA library prep kit for Illumina coupled with NEBNext multiplex oligos for Illumina (New England Biolabs) with few modifications optimized for small fragments (detailed in dx.doi.org/10.17504/protocols.io.wvgfe3w. ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: RNA-seq libraries were generated by using NEBNext ultra II directional RNA library prep kit coupled with NEBNext multiplex oligos for Illumina (New England Biolabs) and following the manufacturer’s directions ...
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bioRxiv - Plant Biology 2021Quote: ... or the 2 kb region upstream of the translational start site of CaPR4 were inserted in front of the GUS reporter gene in vector pRB-bar-GUS using the NEBuilder recombinational cloning kit (NEB).
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bioRxiv - Synthetic Biology 2021Quote: ... an equal volume of RNA lysis buffer was added and RNA isolated using Monarch Total RNA Miniprep Kit (New England Biolabs). Each sample was treated by DNase I twice ...
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bioRxiv - Synthetic Biology 2021Quote: ... We ligated the vector with the inserts through Gibson assembly (Gibson et al., 2009) using NEBuilder HiFi DNA assembly kit (New England Biolabs). All primers and oligos used including sequencing primers (Integrated DNA Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... These four oligos were then assembled with PCR amplified pUA66 vector using NEBuilder HiFi DNA assembly kit (New England Biolabs). The resulting pMV001 plasmid assembly mix was then used to electroporate Top10 E ...
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bioRxiv - Physiology 2021Quote: ... The in vitro transcription of ayRhp1 capped mRNA (cRNA) was performed with HiScribe T7 ARCA mRNA kit (New England Biolabs) on SphI linearized pGEM-HE-ayRhp1 vector followed by column purification (RNeasy MiniElute Cleanup Kit ...
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bioRxiv - Evolutionary Biology 2021Quote: Library preparation was done with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... chromatin from both IP and ‘Input’ samples were eluted and de-crosslinked at 65°C for 16 hours followed by DNA isolation by PCR and DNA cleanup kit (NEB). Enrichment of AR and MED1 at specified enhancers were quantified by qPCR using specific primers ...
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bioRxiv - Microbiology 2019Quote: ... whereas KAPA HyperPlus libraries and libraries sequenced on the Ion Torrent PGM platform were quantified by qPCR using the NEBNext Library Quant Kit for Illumina (New England BioLabs) or the KAPA Library Quantification Kit for Ion Torrent platforms (Figure 1) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we sheared total pooled DNA into ∼300–400 bp fragments in an ultrasonicator and used it to build sequencing libraries with an NEBNext Ultra DNA library prep kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were purified with a 0.8x bead to library ratio and eluted in 30μL EB buffer and qPCR quantified using the NEBNext Library Quant Kit for Illumina (New England BioLabs Inc.). Purified libraries were pooled equimolarly according to the qPCR results and sequenced at the GeoGenetics Sequencing Core ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of RNA was used as starting material to make libraries following manufacturer instructions for NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). Two or more biological replicates were used for making libraries in parallel ...
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bioRxiv - Molecular Biology 2021Quote: An aliquot of the DNA extract was placed into the NEBNext Ultra II Library preparation kit (New England Biolabs®) following manufacturer’s instructions with the following modifications ...
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bioRxiv - Molecular Biology 2021Quote: Samples were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Cat # E7645) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Microbiology 2020Quote: ... and were fused to fragment TRL2(Δgfp)-NH using the NEBuilder HiFi DNA Assembly Cloning kit (NEBuilder, NEB, Cat. #M0491) to create plasmids pTRL2-NH-EUO and pTRL2-NH-HrcA ...
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bioRxiv - Microbiology 2021Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... The RNA-Seq libraries were prepared from total RNA using the NEBNext Ultra II RNA Sample preparation kit (NEB, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Transcription of pgRNA constructs were carried out using a Hiscribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), after linearization of the DNA plasmid using HindIII ...
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bioRxiv - Microbiology 2021Quote: ... The Y-adaptor was ligated to the ends of the fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were gel purified and transcribed in vitro using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). The transcribed sgRNAs were purified using the RNeasyTM mini kit (Qiagen).
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bioRxiv - Cell Biology 2020Quote: InsertTAG-pcDNA FRT/TO was created by mutagenesis of YFP-pcDNA5 FRT/TO39 to replace YFP with NheI and XhoI restriction sites using the primers ccctcgagGATATCACAAGTTTGTACAAAAAAGC (forward) and tggctagcAAACGCTAGAGTCCGGAG (reverse) and the Q5 Site-Directed Mutagenesis Kit (E0554S, New England Biolabs). BioID2-pcDNA5 FRT/TO was created by restriction digestion of BioID2 from myc-BioID2-MCS (74223 ...
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bioRxiv - Genomics 2019Quote: ... Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB). All end preparation ...
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bioRxiv - Molecular Biology 2020Quote: ... Region B or Region C were used as templates for in vitro transcription by HiScribe T7 quick high yield RNA synthesis kit (NEB). The obtained RNA products were converted back to DNA oligo probes via reverse transcription (RT ...
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bioRxiv - Developmental Biology 2019Quote: ... The precipitated DNA fragments were ligated with adaptors and amplified by ten cycles of PCR using NEBNext Ultra II DNAlibrary Prep Kit for Illumina (NEB) to prepare libraries for DNA sequence determination using Illumina HiSeq2500 and single-end reads of 50bp ...
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bioRxiv - Neuroscience 2020Quote: ... Intron-containing dCas9-VPR was constructed by the insertion of a gBlock containing the SV40 intron into the original dCas9-VPR plasmid via Gibson assembly (Gibson Assembly kit, New England BioLabs). The SVI-FLEX construct was built via Gibson assembly of the original dCas9-VPR backbone and two gBlocks encod-ing the SV40 intron sequence ...
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bioRxiv - Molecular Biology 2021Quote: Double-stranded cDNA was prepared for Illumina sequencing with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs) and iTru primers ...
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bioRxiv - Genomics 2021Quote: ... The library was prepared using the ONT 1D kit (SQK-LSK108) and the NEBNext End repair / dA-tailing Module NEB Blunt/TA Ligase Master Mix (New England Biolabs) according to the kit’s manual ...
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bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were prepared from 300 ng of ribo-depleted RNA (NEBNext Ultra II Directional RNA Library preparation kit) and indexed with NEB Next Illumina indexing primers (NEB). An Agilent Bioanalyzer with high sensitivity DNA chip was used to confirm the quality of the libraries before they were sent for sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified fragmented mRNA was then used to generate cDNA libraries for sequencing using NEBNext Ultra Directional RNA library Kit (E7420S, NEB) following the protocol provided by the manufacturer with these modifications ...
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bioRxiv - Immunology 2020Quote: ... In vitro transcription of gRNAs from assembled oligonucleotides was conducted using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). Injected clutches of embryos were validated to contain CRISPR mediated mutagenesis by a T7 endonuclease assay ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragment libraries were prepared by shearing genomic DNA using a Covaris sonicator and the NEBNext Ultra II DNA library preparation kit (New England BioLabs). DNA was sequenced on a ∼40 Gb (i.e ...
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bioRxiv - Cell Biology 2019Quote: ... pSS363 and pSS364 were generated following essentially the procedure described in the Q5 site-directed mutagenesis kit (New England Biolabs) using the pSS75 plasmid as template ...
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bioRxiv - Genomics 2019Quote: ... we introduced the alternative alleles via site-directed mutagenesis (SDM) using the NEB Q5 Site-Directed Mutagenesis kit (New England Biolabs). We designed primers using NEBaseChanger (v.1.2.8) ...
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bioRxiv - Cell Biology 2019Quote: ... The two fragments were then ligated together (via the AatII digest site) using a T4 ligase (kit from New England Biolabs) and subsequently PCR amplified using primers P5 and P11 ...
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bioRxiv - Developmental Biology 2019Quote: ... Extracted DNA was prepared into ChIP-seq libraries using the NEBNext Ultra DNA library preparation kit for Illumina (New England Biolabs) and was sequenced on the HiSeq 2500 platform.
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bioRxiv - Biochemistry 2020Quote: ... at 95°C for 5 min and incubated with 80000 units of O-glycosidase and 100 units of neuraminidases for 4 hours at 37°C following the NEB kit protocol (E0540S; NEB). Samples were resolved on 15% Tris-tricine SDS-PAGE gel and blotted using anti-Cterm OCN goat antibody.
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bioRxiv - Molecular Biology 2019Quote: ... Enriched RNAs were then chemically fragmented and libraries were constructed using the NEBNext small RNA library preparation kit (NEB E7330) as previously described (13) ...
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bioRxiv - Molecular Biology 2019Quote: ... All the pCMV and pcDNA3.1-myc-eIF6 phospho-site mutants were generated using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) using the forward and reverse primers listed in Appendix Table S1 ...
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bioRxiv - Physiology 2019Quote: ... The human equivalent of the c655 mutation (G865V) was introduced into this plasmid using the Q5 Site-directed mutagenesis kit (New England Biolabs), with the following primer pair ...