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Citations for New England Biolabs :
9151 - 9200 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... These PCR products were inserted into EcoRI-XhoI-digested linearized pCAGGS using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Ipswich, MA, USA). Previously constructed plasmids expressing G of VSBV-1 ...
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bioRxiv - Microbiology 2024Quote: ... DNA segments exceeding 1 kb in length were manually excised from the agarose gel and retrieved using a Monarch® DNA Gel Extraction Kit (NEB Inc., USA). A Qubit 2.0 Fluorometer and the Qubit (ds)DNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... high molecular weight (HMW) DNA was extracted from an overnight culture using the Monarch HMW DNA Extraction Kit for Tissue (New England Biolabs, Ipswich, MA, USA) as per manufacturer’s protocol for HMW DNA extracted from bacteria ...
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bioRxiv - Developmental Biology 2024Quote: ... and the sgRNA was synthesized in vitro as described in the manual (HiScribe™ T7 High Yield RNA Synthesis Kit, NEB, NO. E2040S), the sgRNA and Cas9 protein were mixed and coinjected into the cell cytoplasm at the one-cell stage ...
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bioRxiv - Developmental Biology 2024Quote: ... dilution of the universal adaptor and 13 cycles (or 16 cycles) of PCR per the respective masses with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Cat. No. E7760L), the NEBNext Poly(A ...
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bioRxiv - Neuroscience 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). The purified double-stranded cDNA fragments were end repaired ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were constructed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina according to manufacturer’s instructions (NEB - catalogue no. E7645S). Library PCRs were supplemented with 2x SYBR dye (Sigma – catalogue no ...
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bioRxiv - Genetics 2021Quote: ... Bands corresponding to small RNAs were collected and their content was recovered using the Monarch DNA Gel Extraction kit (NEB #T1020 New England Biolab). The sRNA collected were turned into libraries using the NEBNext Multiplex Small RNA Library Prep Set (NEB #E7300S New England Biolab) ...
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bioRxiv - Genetics 2021Quote: ... The entire alh-6 genomic sequence (ATG to stop) was amplified by PCR and cloned in a linearized pMiniT 2.0 vector (NEB PCR Cloning Kit, Cat. #E1202S). Plasmid DNA was purified using the Zymo Research Zyppy Plasmid Miniprep kit (Cat ...
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bioRxiv - Microbiology 2021Quote: ... The RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) at Genotypic Technology Pvt ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration was determined using a Nanodrop and libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Molecular Biology 2021Quote: ... following the manufacturer’s instructions and libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Evolutionary Biology 2021Quote: Extracted and purified RNA was reverse transcribed into first-strand copy DNA (cDNA) using the ProtoScript® II Reverse Transcriptase Kit (NEW ENGLAND BioLabs®Inc, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation was conducted using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Strand-specific RNASeq libraries were created using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #6420L; New England Biolabs, Inc., Ipswich, MA, USA). The library sizes and quantification were determined using the Bioanalyzer High Sensitivity DNA kit (Cat# 5067-4626 ...
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bioRxiv - Molecular Biology 2021Quote: ... original numbering with signal sequence = 20–559) were subcloned into the manufacturer-supplied plasmid from the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) using the Gibson assembly method with synthetic E ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed from 2.5 ng of DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 9 PCR cycles (NEB #E7645S, New England Biolabs). Library quality was assessed by High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Analytical amounts of twenty Argonaute proteins were synthesized from pET29a plasmids using PURExpress In Vitro Protein Synthesis kit (New England Biolabs, Inc., Ipswich, MA, USA). For large scale expression and purification of CbAgo ...
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bioRxiv - Genomics 2020Quote: Whole Genome DNA libraries with an average insert size of 200 bp were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, 2016) using the protocol recommended by the company ...
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bioRxiv - Genomics 2020Quote: ... Approximately 300 ng of genomic DNA was used to prepare DNA sequencing libraries for each parent following the protocol of NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB Cat No. #E7805S/L). The libraries were sent to Novogene (https://en.novogene.com/ ...
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bioRxiv - Microbiology 2019Quote: ... sequencing libraries were generated from rRNA-depleted RNA using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: Shotgun libraries for Illumina MiSeq sequencing were prepared with the NEBNext® Ultra II DNA Library Prep Kit for Illumina® in combination with the Index Primer Set 1 (NEB, Frankfurt, Germany) according to manufacturer’s instructions and as described in (57) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The library was then PCR amplified in five separate aliquots (12 cycles, Phusion High Fidelity® PCR kit, New England Biolabs Inc., Frankfurt, Germany). We used Agencourt® AMPure XP beads (Beckman Coulter ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and transcribed after subcloning into the DR274 vector using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). The Cas9 protein was produced in Escherichia coli as previously described (Ménoret et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA samples were used for RNA-Seq library construction using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA). We followed the protocol for normal insertion size option.
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bioRxiv - Genomics 2019Quote: ... Two strand-specific RNA-seq libraries with an insert size of 350 bp using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) were prepared and sequenced on an Illumina NovaSeq 6000 platform ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of RNA was used for cDNA library construction using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA libraries were generated by the “dUTP” method for strand-specificity using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs, E7760S, E7335). Library size (~350 bp ...
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bioRxiv - Plant Biology 2021Quote: The RNA-seq libraries were constructed with the NEB Next® Ultra™ II RNA Library Prep Kit for Illumina® following manufacturer’s instruction (NEB, Cat#E7775).
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bioRxiv - Plant Biology 2020Quote: ... A total of 12 sRNA libraries were constructed from 5 μg of size-selected RNA using the NEBNext® Small RNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) as per the manufacturers’ instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Libraries were prepared from 1 μg of total RNA using NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) by poly(A ...
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bioRxiv - Genomics 2019Quote: The genomic DNA was extracted from 10 mg frozen brain cortex samples using NEB Monarch genomic DNA® purification kit (NEB, Ipswich, MA, USA). Four microliters of RNase A (100mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were generated from a total amount of 3 μg RNA per sample using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Genetics 2021Quote: ... The PCR product was inserted to the PstI and BglII sites on the pWALIUM20 vector (Perkins 2015) by Gibson-assembly (NEB HiFi DNA Assembly Kit) to generate pWALEXA20 (White-AttB-LexAop for shRNA expression) ...
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bioRxiv - Microbiology 2021Quote: ... D186N were first introduced into the Rluc-CV-B3 reporter viruses with Q5 site-directed mutagenesis kit (New England Biolabs, Bioké, Leiden, The Netherlands)52,53 ...
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bioRxiv - Microbiology 2019Quote: ... Metatranscriptomic libraries were prepared from total RNA using the NEBNext® Ultra II Directional RNA Sequencing Prep Kit (New England BioLabs, Ipswich, MA, USA) in conjunction with the NEBNext® rRNA Depletion Kit for Human/Mouse/Rat (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: Metagenomic libraries were prepared from DNA (100 ng) using the NEBNext® Ultra II FS DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA) > 100ng input protocol as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... We inserted TFT reporters into BFA0190 by digesting the plasmid with SwaI and inserting TFT components between the LYP1 flanking sequences using isothermal assembly cloning (Hifi Assembly Cloning Kit; New England Biolabs [NEB], Ipswich, MA, USA). The 5’ and 3’ LYP1 flanking sequences in each TFT plasmid contain natural SacI and BglII restriction sites ...
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bioRxiv - Molecular Biology 2022Quote: ... A cDNA library was constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of total RNA was used for intact RNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760, New England BioLabs). To obtain sufficient RNA for the analysis of oral samples ...
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bioRxiv - Genomics 2022Quote: ... cDNA libraries were prepared the same day using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB E7760). All samples were amplified using 8 cycles of PCR.
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bioRxiv - Genetics 2022Quote: ... with insert-specific primers LC186 and 187) between hlh-30 and 3XFLAG-tag segments with HiFi cloning (NEBuilder® HiFi DNA Assembly Cloning Kit, New England BioLabs Inc., Ipswich, MA). Lastly ...
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bioRxiv - Cancer Biology 2022Quote: ... CUT&RUN barcoded libraries for Illumina sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645) accordingly with Zhu et al.(Zhu et al. ...
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bioRxiv - Plant Biology 2021Quote: ... and phloem tissues were also prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs®) and barcoded using index primers from the NEBNext® Multiplex Oligos for Illumina® (New England BioLabs®) ...
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bioRxiv - Genomics 2020Quote: Genomic DNA purified from one single haploid drone of each species was used to generate one “fragment” library with an insert size of 400 or 450 bp using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA). The prepared fragment libraries were sequenced on an Illumina HiSeq 2500 platform with a read length of 250 bp to produce overlapping paired-end shotgun reads (2 × 250 bp) ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (cat no. E7770, New England Biolabs, Ipswich MA). Single-end sequencing was performed using the Illumina NextSeq500 platform according to standard operating procedures.
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bioRxiv - Immunology 2021Quote: ... Master mix preparation and cycling conditions were realized with Luna® Universal qPCR Master Mix kit (cat. n° M3003E, NEB, New England Biolabs, Ipswich, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Illumina MiSeq adaptors were ligated onto the amplicon library using a NEBNext® DNA MasterMix for Illumina Kit (New England BioLabs, Ipswitch, MA, USA) and sequences were generated using a MiSeq instrument (2 × 250 cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries for ChIP-seq were constructed using NEBNext® UltraTM DNA Library Prep Kit for Illumina (Cat. #E7370; New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The extracted DNA from water and soil underwent a further purification step using Monarch®PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA). DNA Library preparation was conducted using Native barcoding genomic DNA Kit SQK-LSK 109 combined with EXP-NBD104 (Oxford Nanopore Technologies ...