Labshake search
Citations for New England Biolabs :
9101 - 9150 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... RNA synthesis was conducted via in vitro transcription using a HiScribe™ T7 high yield RNA synthesis kit (New England BioLabs, NEB, Ipswich, MA). After DNase I (RNase-free ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries for RNA-Seq were prepared with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England BioLabs, USA) for three replicates per treatment resulting in 24 libraries with a mean insert size of 300 bp ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutagenesis of ATG8-Interacting Motifs (AIM) was performed using Q5 site-directed mutagenesis kit (New England BioLabs, Frankfurt am Main, Germany) using primers listed in Supplemental Table S5 according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The purified DNA was amplified for high-throughput sequencing by an Ultra II DNA Library Prep Kit (New England Biolabs, Catalog Number: E7645S). Then ...
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bioRxiv - Physiology 2023Quote: mRNA was extracted from mesenteric and renal artery whole cell lysates using the Monarch Total RNA Miniprep Kit’ (New England Biolabs, Ipswich, Massachusetts, USA). The concentration and purity of the RNA samples were calculated using a NanoDrop 3300 Fluorospectrometer with absorbance set to 260nm and 280nm ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA was cloned using either classical restriction and ligation ([55]; restriction enzymes, T4 DNA ligase and Quick Ligation kit from New England Biolabs, Ipswich, Massachusetts, USA) or isothermal assembly ([56] ...
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bioRxiv - Plant Biology 2024Quote: 5 μl of ribosomal depleted RNA from each sample were used to prepare 64 individually barcoded RNA-seq libraries using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was extracted from 30-35 sets of MTs isolated from 5-7-day old female flies in five independent biological replicates using the Monarch Total RNA Miniprep kit (New England BioLabs, Whitby, ON, Canada). Total RNA was quantified using a UV spectrophotometer (Synergy 2 microplate reader ...
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bioRxiv - Immunology 2023Quote: Library preparation and sequencing were performed by Novogene UK using the NEB Next Ultra RNA Library Prep Kit (New England BioLabs, Cat. No. E7530L). Samples were sequenced using 150-bp paired-end reads using NovaSeq 6000 (Illumina).
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Molecular Biology 2023Quote: ... At day 4 post nucleofection genomic DNA was extracted and 500 ng of gDNA from each sample were randomly digested with NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E6177) to obtain fragments of ca ...
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bioRxiv - Microbiology 2023Quote: ... PCR was conducted using a Rapid Barcoding Kit 96 (ONT, Oxford, UK) with Q5® Hot Start High-Fidelity 2X Master Mix (NEB, Ipswich, MA) according to the manufacturer’s protocol designed for SARS-CoV-2.
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bioRxiv - Biochemistry 2023Quote: ... was constructed by replacing the gRNA region in pPvU6b-DmtRNA-BbsI (16) using the NEBuilder HiFi Assembly kit (New England BioLabs, Ipswich, MA, USA) as previously described (16 ...
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bioRxiv - Bioengineering 2023Quote: ... and whole mRNAseq libraries were generated using the NEB Next® UltraTM Nondirectional RNA Library Prep Kit for Illumina® (New England Biolabs, MA), following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs, NEB) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and the sequencing libraries were prepared using the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760). The sequencing libraries were prepared following Chapter 1 of the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs ...
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... the DNA pellet was resuspended in 0.1 X TE and used for DNA library generation with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs). Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit) ...
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bioRxiv - Cell Biology 2023Quote: ... Adaptor ligation was performed with 1:25 diluted adaptor and 15 cycles were used for library amplification using dual indices (NEB dual index kit). Paired-end 2×25 bp sequencing was performed on a NextSeq500 Illumina Sequencer.
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bioRxiv - Microbiology 2023Quote: ... Extracted DNA was used for library preparation with NEB Next® Ultra IITM DNA Library Prep Kit for Illumina® (New England Biolabs, USA) and sequenced on Illumina NovaSeq 6000 platform (150 bp ...
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bioRxiv - Genomics 2023Quote: ... a standard whole-genome 150 base pair (bp) paired-end Illumina library was prepared using the NEBNext Ultra II library preparation kit (New England Biolabs Inc., Ipswich, USA). Finally ...
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bioRxiv - Genomics 2023Quote: ... We then ligated barcodes to the end-prepped DNA using the Native Barcoding Expansion 1–12 kit (EXP-NBD104, Oxford Nanopore Technologies, Oxford, UK) and Blunt/TA Ligase Master Mix (New England Biolabs, Ipswich, MA, USA). We cleaned the barcoded samples using 0.9X AMPure XP beads ...
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bioRxiv - Genomics 2023Quote: ... using the Adapter Mix II from the Native Barcoding Expansion 1–12 kit and NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA, USA) as well as Quick T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... the transcriptome sequencing library was prepared and sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). All samples were clustered using the TruSeq PE Cluster Kit v3 cBot HS (Illuminia ...
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bioRxiv - Neuroscience 2023Quote: ... RNA Library preparations for Synpo-related transcriptome analysis were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Polyadenylated RNA enrichment and library preparation were conducted using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (NEB, #E7490S and #E7760S), respectively ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (E7770, New England BioLabs, Ltd., USA) by the Beijing Allwegene Technology Company Limited (Beijing ...
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bioRxiv - Bioengineering 2024Quote: ... Synthetic RNA was generated through in vitro transcription of gene fragments using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and quantified via qPCR standard curve.
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bioRxiv - Systems Biology 2024Quote: ... The poly(A) mRNA-enriched libraries were constructed using NEBNext® Ultra™ II RNA Library Prep Kit (New England BioLabs, Ipswich, MA). Paired-end sequencing (2×150 bp ...
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bioRxiv - Microbiology 2023Quote: ... A second PCR purification was performed prior to insertion of the Ultramers into the vector by DNA assembly with the NEBuilder HiFi DNA Assembly Kit (NEB, Cat. No. E2621). Next ...
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bioRxiv - Genomics 2024Quote: ... 5 ng of the resulting RNA was applied for library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New Englands Biolabs).
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bioRxiv - Microbiology 2024Quote: ... RNA was synthesized from a DNA template (Supplementary Table 3) using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA) and treated with DNase I (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was submitted for small RNA sequencing to the UT Southwestern McDermott NGS Core using the NEBNext Small RNA kit (New England Biolabs, cat. no. E7330), with size selection between 15bp and 30bp ...
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bioRxiv - Genetics 2024Quote: To determine the T-DNA insertion site of line ClvRubq7 we extracted genomic DNA from two different plants and constructed sequencing libraries using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E7805) following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 5 μg of HMW DNA sample was fragmented by sonication to a size of 350 bp and sequencing library was prepared using NEB Next® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs, USA) following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently used to assemble the RNA-seq library using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB; E7760), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Amplicons prepared for long-Illumina read sequencing (PE300) underwent end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA). Custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix with a 15 min incubation at 20°C (Supplemental Table 6) ...
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bioRxiv - Microbiology 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). The purified double-stranded cDNA fragments were end repaired ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified ChIP DNA and 220 ng of whole cell extract DNA were used to prepare ChIP-seq libraries using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645S) with PCR amplifications carried out for 16 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... 800ng RNA was used per sample as input for the RNA library preparation using NEBNext® rRNA Depletion Kit v2 (NEB, E7405L, USA) and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... a total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations and sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA).
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bioRxiv - Cell Biology 2024Quote: ... to pull down mRNA and used for cDNA library construction using NEBNext ultra II directional RNA library prep kit for Illumina as recommended by the manufacturer (New England Biolabs, Inc, Ipswich, MA). The quality of the cDNA libraries were assessed on the TapeStation (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... The precipitated DNA was washed once with 70% ethanol and suspended in 30μl 0.1 X TE (1mM Tris-HCl and 0.1mM EDTA) for library preparation with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The two fragment products of rpfF and the linearized pK18mobsacB (Schäfer et al. 1994) were then isothermally assembled using the Gibson cloning kit (NEB; Gibson et al. 2009) following the manufacturer’s instructions in a 5:1 insert to backbone ratio ...
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bioRxiv - Cell Biology 2024Quote: ... GFP mRNA was produced in-house using the Hiscribe® T7 Quick high yield RNA synthesis kit and 3’-O-me-m7G cap analog (New England Biolabs, MA, USA) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). The resulting double-stranded cDNA fragments were subjected to end repair ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was used as the template for IVT with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... by Gibson assembly using the NEBuilder® HiFi DNA Assembly kit and following the manufacturer’s instructions (New England BioLabs Inc., Ipswich, MA, USA). The resulting Gibson assembly products were transformed into 50 µl of DH5α competent E ...
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bioRxiv - Biochemistry 2024Quote: 1μl of total cellular RNA was used for sequencing library generation by NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A library was then prepared following the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit procedure (New England Biolabs, Ipswich, MA, USA). A library was directly prepared from 100 ng of total RNA extracted from floral buds using the NEBNext Ultra II Directional RNA Library Prep for Illumina Kit ...