Labshake search
Citations for New England Biolabs :
9101 - 9150 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: RNAseq libraries were prepared with the “protocol for library preparation of Intact RNA using NEBNext rRNA depletion kit (Bacteria) (NEB#E7850, NEB#78860) and NEBNext Ultra II directional RNA library prep kit for Illumina (NEB#E7760 ...
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bioRxiv - Genetics 2021Quote: ... PCR products were assembled and cloned into the pW8-dhdWT vector (Tirmarche et al., 2016) previously digested by KpnI and BamHI using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, #E5520S). dhdΔDRE transgene was integrated in the PBac{attP-3B}VK00031 platform (62E1 ...
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bioRxiv - Genetics 2020Quote: ChIP-seq libraries were built using the NEB Next UltraII DNA library Prep kit for Illumina (New England Biolabs #E7645S/L) and Agencourt Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2019Quote: ... as shown in figure S2) were introduced into the ARSA plasmid by using Site-Directed Mutagenesis Kit (Cat. No. E0552s, NEB, USA). The sequences of mutated cDNA vectors were confirmed using the ABI3500 sequencer (Applied Biosystems Inc. ...
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bioRxiv - Genomics 2021Quote: ... The transcriptome library was prepared using NEB ultraII RNA library preparation kit as per the manufacturer’s recommended protocol (NEB, MA, USA) and the libraries then underwent size selection ...
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bioRxiv - Genomics 2021Quote: ... 1μg of total RNA was used to enrich mRNA using NEB Magnetic mRNA isolation kit according to the manufacturer’s instructions (NEB, MA, USA). cDNA was synthesized and ligated to sequence adapters ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitated DNA was reverse-crosslinked and used to construct high-throughput sequencing libraries using NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370). DNA libraries were processed on a Illumina HiSeq machine for single-end sequencing ...
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bioRxiv - Genetics 2020Quote: ... pREP81-FLAG vector and plasmids carrying wild type and mutated forms of rrp1+ and rrp2+ as well as domains of rrp1+ gene used in the yeast two hybrid system were constructed using the Gibson Assembly® Cloning Kit (NEB). All primers used to amplify gene sequences by PCR are listed in Table S3 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and used this as template to construct DNA libraries using a NEBNext Fast DNA Library Prep Set for Ion Torrent kit (New England Biolabs, Canada). Each sample library contained a unique barcode adapter (NEXTflex DNA Barcodes for Ion Torrent ...
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bioRxiv - Microbiology 2020Quote: ... Shotgun metagenomic sequencing libraries were prepared directly from one aliquot of each sheared genomic DNA sample using the NEBNext Illumina library preparation kit according to the manufacturer’s directions (New England Biolabs, MA, USA). Samples were then sequenced with 2×250 bp cycles of v2 Miseq chemistry (Illumina ...
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bioRxiv - Genomics 2021Quote: This material was used for generating sequencing libraries using the Small RNA library prep Set for Illumina (Multiplex compatible) kit (NEB E7330). Typically ...
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bioRxiv - Genomics 2021Quote: ... The resulting rRNA-depleted RNA was used for library construction with the NEB ultra II directional RNA-seq kit (NEB E7760). The library was sequenced on illumina Miseq (75 bp paired-end sequencing) ...
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bioRxiv - Genomics 2021Quote: ... and a portion corresponding to 1 µg input RNA was converted to cDNA using the LunaScript RT SuperMix cDNA synthesis kit (NEB E3010). 1% of the cDNA was used for each qPCR reaction performed with the Luna Universal qPCR Mastermix (NEB M3003 ...
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bioRxiv - Genomics 2021Quote: ... The elution product was directly used for second-strand synthesis using reagents from the NEBNext Ultra II Directional RNA Library Prep Kit (NEB E7760). 8 μL of Second Strand Synthesis Reaction Buffer ...
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bioRxiv - Genomics 2020Quote: ... Following the manufacturer’s instruction 3μg RNA per group was used for sequencing libraries were generated using a NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). The index-coded samples were clustered on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Libraries for ChIP-seq were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, NEB #E7370) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the NEB Library Prep protocol of the manufacturer was followed and samples were adaptor ligated using the NEBNext End Prep kit (NEB, #E7645). The adaptor-ligated DNA fragments were indexed with the NEBNext Multiplex Oligos for Illumina kit (NEB ...
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bioRxiv - Immunology 2021Quote: Point or deletion mutations were prepared from human or mouse CRT expression clones in pCMV3-C-Myc (SinoBiological) using the Q5® Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol and primers designed using the NEBaseChanger™ web tool ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA libraries were prepared at Génome Quebec (Montreal, QC, Canada) with the Ultra II NEB DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). Library quality and size distribution was determined on a high-sensitivity DNA chip on the Agilent 2100 bioanalyzer system ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were prepared on a template of randomly fragmented mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA). Since 8 samples out of 20 initially contained < 200 ng of total RNA ...
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bioRxiv - Microbiology 2021Quote: ... The resultant PCR fragments were inserted into the BamHI site in pAK405 by NEBuilder HiFi DNA assembly cloning kit (New England Biolabs, Inc.). These plasmids were independently introduced into SYK-6 and its mutant cells by triparental mating ...
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bioRxiv - Biochemistry 2020Quote: ... molecules used in this study were synthesized by in vitro transcription using HiScribe™ T7 Quick High Yield RNA Synthesis Kits (New England Biolabs), or transcribed directly in the in vitro translation (IVT ...
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bioRxiv - Bioengineering 2019Quote: ... The linear PCR product was treated with DpnI enzyme to fragmente the template plasmid and self-ligated to generate circular plasmid (Quick Ligation™ Kit, NEB). Promoters containing multiple trpO sequences were constructed by USER cloning from a synthetic DNA fragment (Integrated DNA Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: Illumina sequencing libraries were generated from 100-200 ng BAC DNA using NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs E7805S) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The BAG1 promoter fragment and EGFP ORF or mCherry (ORF) were cloned by NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S) into the vector backbone that was produced by double enzymatic digestion of pTUB1:YFP-mAID-3HA ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations to the binK sequence on the plasmid were designed using the NEBaseChanger tool and constructed with the Q5 site-directed mutagenesis kit (New England BioLabs, Inc.). The constructed plasmid was transformed into either electrocompetent or chemically competent DH5α λpir E ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared using the rRNA-depleted RNA as input for NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA). Libraries were pooled and submitted for 2 × 150 bp paired-end sequencing on an Illumina NextSeq High-Output platform at the Center for Genome Sciences and Systems Biology at Washington University in St ...
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bioRxiv - Developmental Biology 2021Quote: ... using 200 ng of sheared gDNA and 10 PCR cycles using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The DNA libraries were indexed with unique dual barcodes (8bp long) ...
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bioRxiv - Cell Biology 2021Quote: Samples were isolated in RNA extraction buffer and RNA isolated using the Monarch® Total RNA extraction Miniprep kit (T2010S; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Spike-in RNA of the bacteriophage MS2 served as an interal control and was detected with Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) using corresponding primers and probe (MS2_F ...
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bioRxiv - Bioengineering 2020Quote: ... The pool of partially double stranded DNA templates was used for in vitro transcription with HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England BioLabs) and the RNA was purified with TRizol (Ambion) ...
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bioRxiv - Bioengineering 2021Quote: ... palustris cultures at mid-exponential phase in duplicate using the Monarch® Genomic DNA Purification Kit (New England Biolabs® Inc.). Amplified PCR product using primers in Supplementary Table VI was diluted to 109 copies of amplicon/μL per the following equation.
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bioRxiv - Biochemistry 2020Quote: ... the sGFP sequence was deleted and a 6xHis-tag placed at the C-terminus of AcrB followed by a stop codon using the Q5® site-directed mutagenesis kit (New England Biolabs) - AcrB contains two Histidine residues at its C-terminus ...
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bioRxiv - Biochemistry 2020Quote: ... The G288D mutation was then generated from this pET15b-AcrB-6xHis plasmid using the Q5® site-directed mutagenesis kit (New England Biolabs). All constructs were verified by DNA sequencing (Eurofins MWG).
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bioRxiv - Biochemistry 2020Quote: ... The G288D mutation was generated from this pBR-AcrBWT plasmid using the Q5® site-directed mutagenesis kit (New England Biolabs). All constructs were verified by primer walking DNA sequencing (Eurofins MWG).
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bioRxiv - Biochemistry 2021Quote: Illumina sequencing libraries were prepared from 1ng to 10ng of purified CUT&RUN DNA using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs # E7645) as previously (Liu et al ...
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bioRxiv - Biochemistry 2020Quote: ... PCR products were cloned into pET22b-mCherry vector using the NdeI and SpeI restriction sites and joined using Gibson Assembly® Cloning Kit (New England Biolabs) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... to obtain the amplified PCR product which was then purified (Quiagen Gel purification kit) and digested with Nco1 and BamH1 restriction endonucleases (NEB, India) for inserting the gene into the pTrc99A expression vector ...
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bioRxiv - Biophysics 2021Quote: ... Mutagenic PCR to obtain the D614G amino acid change into both untagged and 161/345A4-tagged SΔTM constructs was done using the primers S2_D614_Q5-F and S2_D614_Q5-R (Table S1) and the Q5® Site-Directed Mutagenesis Kit (NEB®, Ipswich, MA, USA) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were prepared using with 1 μg of total RNA and mRNA isolation step was done by using NEBNext poly(A) mRNA Magnetic Isolation kit (New England Biolabs, E7490S). The cDNA libraries were generated by the “dUTP” method for strand-specificity using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... and the DNA-free samples consisting of RNA were converted to cDNA using M-MuLV Reverse Transcriptase kit (New England BioLabs, USA) according to the instructions provided by the supplier ...
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bioRxiv - Cell Biology 2021Quote: ... RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich MA). The sequencing library was validated on the Agilent Tape Station (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were chosen to minimize predicted off-target effects with maximum cleavage efficiency and were synthesized by in vitro transcription as previously described26 using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S) or ...
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bioRxiv - Cancer Biology 2020Quote: ... The Nup88 construct lacking the coiled coil domain (Nup88∆C) was created by inserting a stop codon after 584aa through site directed mutagenesis (Q5 Site Directed Mutagenesis Kit, NEB-E0554S) using pEGFPC1-Nup88 as template ...
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bioRxiv - Cancer Biology 2020Quote: ... was digested with BbsI and the gRNAs targeting E6 and E7 genes from HPV18 and HPV16 were designed using NEBuilder® HiFi DNA Assembly cloning kit (NEW ENGLAND BioLabs). Briefly ...
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bioRxiv - Cell Biology 2021Quote: The components of both cassettes were first PCR-amplified from other vectors and subsequently ligated together using the NEBuilder® HiFi DNA Assembly Cloning kit (NEB) to generate the resulting pENTR-eGFP-attP(bxb)-*BsdR and pENTR-mCherry-attP(C31)-*BleoR vectors ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The single-stranded labeled probes were finally cleaned up using the Monarch PCR & DNA cleanup kit (New England Biolabs®, T1030) following the oligonucleotide cleanup protocol ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was stored short-term at −20°C or long-term at −150°C or immediately used in a retrotranscription reaction (ProtoScript® AMV First Strand cDNA Synthesis Kit, New England Biolabs or SuperScript® III First-Strand Synthesis System ...
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bioRxiv - Neuroscience 2021Quote: ... Point variants were introduced into hFibcd1 cDNA while cloned in the intermediate pDONR201 plasmid using the Q5 site-directed mutagenesis kit (NEB #E0554), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA, USA inc.) for Illumina following the manufacturer’s protocol ...