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Citations for New England Biolabs :
851 - 900 of 2232 citations for Rat SEPT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... we performed test-digestion of the generated plasmids with restriction enzymes (New England Biolabs), and analyzed the fragment pattern via gel electrophoresis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All plasmids were cloned into high-efficiency chemically competent DH5α (NEB, Ipswich, MA, USA). Plasmids were purified using PureYield miniprep kits (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: ... The entire plasmid was PCR amplified using Q5 DNA Polymerase (New England BioLabs Inc.) with the following degenerate primers where N indicates any base ...
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bioRxiv - Microbiology 2021Quote: ... These plasmids were generated using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554) instructions based on the previously described codon-optimized pD10-3xFlag according to the manufacturer’s [35] ...
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were recovered following linearization of infectious clone plasmids using NotI-HF (NEB), in vitro transcription ...
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bioRxiv - Genomics 2021Quote: ... ENGRAM plasmid and PCR product from above were digested with BsmBI (NEB, buffer 3.1) at 55°C for 1h and purified for ligation ...
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bioRxiv - Microbiology 2021Quote: ... acnes lysates were incubated with 0.5 ug plasmid DNA together with different buffers (NEB) supplemented with or without ATP or SAM ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid pMTL83152::Fncas12a was first digested using BbvCI and CIP (New England Biolabs) for 3h at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... gBlocks fragment and backbone plasmid was ligated by T4 DNA ligase (NEB cat# M0202S) at 16 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... were isolated using the Monarch® Plasmid Miniprep Kit (New England BioLabs Inc., USA), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The TPCR-amplified plasmid DNA was treated with DpnI (New England Biolabs, Ipswich, MA) to remove any parental plasmid used as a template to construct the library ...
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bioRxiv - Genomics 2022Quote: ... 8ug of STARR-seq plasmid was digested with 20uL AgeI-HF® (R3552, NEB), 20uL SalI-HF® (R3138 ...
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bioRxiv - Biochemistry 2022Quote: ... All other plasmids were cloned by Gibson Assembly using NEBuilder HiFi (New England Biolabs). Mutations were introduced using primers containing the desired mutations ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid cloning and multiplication were performed in Escherichia coli DH5α (New England Biolabs, USA). E ...
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bioRxiv - Microbiology 2022Quote: ... All DNA amplicons for plasmids constructs were amplified with high fidelity Phusion Polymerase (NEB) to minimize replication errors ...
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bioRxiv - Molecular Biology 2022Quote: ... cloned into the pCRISPRia-v2 plasmid digested with BstXI and BlpI (New England Biolabs). Following the sgRNA transduction and puromycin selection ...
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bioRxiv - Cell Biology 2022Quote: ... plasmid clones containing the correct insert were linearized with DraI (#R0129S, New England Biolabs) and sgRNA was synthesized in vitro using a Maxiscript T7 Transcription Kit (#AM1312 ...
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bioRxiv - Molecular Biology 2020Quote: All plasmids were cloned using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and mutations were made using the Q5 Site-directed mutagenesis kit (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Digested plasmid was then de-phosphorylated with Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Most of the plasmids were assembled using the NEBuilder HiFi DNA assembly mastermix (NEB) following manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... The inserts were cloned into pLRE::GFP plasmid linearized with BamHI (NEB, Catalog # R0136) and SalI (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Single gRNAs were cloned into LentiCRISPR 2.1 plasmid [57] by BsmBI (New England BioLabs) digestion and Gibson Assembly (New England BioLabs) ...
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bioRxiv - Neuroscience 2020Quote: ... The ensuing plasmid was then mutated using the New England Biolabs (NEB, Ipswich, MA) mutagenesis Q5 kit in 29 different reactions ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids were amplified by transformation into chemically competent Escherichia coli DH5α (New England Biolabs), which was plated onto LB agar (10 g/L tryptone ...
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bioRxiv - Biophysics 2019Quote: ... we performed a double digest of TH1007 and the pSNAPf plasmid (New England Biolabs) with AgeI and BamHI ...
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bioRxiv - Molecular Biology 2020Quote: ... and inserted into the opened plasmid with NEBuilder reagents (New England Biolabs; Berverley, MA) to create plasmid pBbRad51NLHAglmS (pBRNLHg ...
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bioRxiv - Biochemistry 2019Quote: ... Nanobody and opto-nanobody plasmids were transformed into Shuffle T7 Express E.coli cells (NEB) and eGFP and mCherry plasmids were transformed into One Shot Top 10 cells (Invitrogen) ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into an SEC backbone plasmid (NEBuilder HiFi DNA Assembly Master Mix, NEB). The modified Cas9/sgRNA plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... This U6-2xBsmBI-Tracr-HygroR plasmid was digested with the BsmBI restriction enzymes (NEB), after which the CRISPR inserts were ligated in using T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... The subcloned AGO plasmids were transformed into Escherichia coli BL21 (DE3) (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were assembled using Gibson assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB E5520S). Fragments were either PCR amplified with a least 15 bp overlap at each end (Phusion Hot Start Flex NEB M0535L ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR was performed on plasmid DNA with Phusion High Fidelity PCR Kit (NEB). PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB ...
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bioRxiv - Molecular Biology 2019Quote: All MKRN1 mutant plasmids were generated with the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... The purified linear plasmid was then dephosphorylated using Alkaline Phosphatase Calf Intestinal (CIP) (NEB) to ensure the linear plasmid did not ligate with itself ...
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bioRxiv - Microbiology 2021Quote: ... and inserted to the intermediate plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB). The L-sNTurboID fragment was ligated back to the pCEZ-L backbone using the same restriction sites Pac1 and Hpa1 by NEB T4 ligase.
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bioRxiv - Cell Biology 2022Quote: ... Ten micrograms of plasmid template were linearized by restriction with MluI (New England Biolabs) and purified using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel) ...
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bioRxiv - Biophysics 2021Quote: ... A plasmid containing two BsaI sites was first treated with BsaI (New England Biolabs), then separated on a 1% agarose gel ...
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bioRxiv - Cell Biology 2021Quote: ... Assembly of the final donor plasmid was performed by Gibson Assembly (New England BioLabs). sgRNA targeting near the 3’-end stop codon of FOXO3 were designed using CHOPCHOP (http://chopchop.cbu.uib.no ...
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bioRxiv - Microbiology 2020Quote: ... The amplicon was cloned in the pXDC39 plasmid vector using XbaI (New England Biolabs) and PstI (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... plantarum B21 plasmids using OneTaq® 2X Master Mix with Standard Buffer (NEB, USA) in a T100™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Derivatives of this plasmid were made using a Q5 site-directed mutagenesis kit (NEB). Single copy deaD-lacZ fusions were made by recombining the plasmid-borne fusions with modified λ phage ...
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bioRxiv - Genetics 2020Quote: Repair template plasmids were assembled from overlapping fragments using the NEBuilder HiFi Kit (NEB) per manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCS2-Rab11S25N-mCherry plasmid was generated using Q5 Site-Directed Mutagenesis Kit (NEB) with PCS2-Rab11-mCherry plasmid and Rab11S25N-mCherry-Fwd (TGTGGGGAAGaatAACCTGCTGT ...
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bioRxiv - Cell Biology 2022Quote: ... pRS306-GFP-linker-CDC42 plasmid was linearized with PshAI (Cat#R0593S, New England Biolabs) and co-transformed with a PCR product containing the desired K-to-R changes into an uracil auxotrophic strain (PC538) ...
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bioRxiv - Immunology 2022Quote: HIV envelope plasmids were transformed and amplified in NEB-stable competent cells (NEB, C3040H). Pseudoviruses were produced by co-transfection of plasmids encoding various HIV-1 Envs together with NL4-3 ΔEnv or Q23-ΔEnv (1:3 ratio ...
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bioRxiv - Synthetic Biology 2022Quote: ... and plasmids were constructed by using Q5 High-Fidelity DNA polymerase (New England Biolabs) to produce amplicons and ligating amplicons to PCR amplified vectors using Golden Gate DNA assembly.59 The broad range RSF1010 backbone was used for all plasmids and was given generously by Shyam Bhakta.60 All plasmid sequences were verified with Sanger sequencing.
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were digested with appropriate restriction enzymes (New England BioLabs, Ipswich, MA, USA) under optimal conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... and inserted into the pGLuc Mini-TK 2 Gaussia luciferase enhancer reporter plasmid (NEB) via KpnI/SacI restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... using a pCAGGS plasmid digested with EcoRV-HF and HindIII-HF (New England Biolabs) as backbone ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids were constructed using standard restriction enzyme cloning or Gibson assembly (New England BioLabs). All plasmids used in this work are described in Supplementary file 2.