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Citations for New England Biolabs :
801 - 850 of 2232 citations for Rat SEPT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The plasmids were linearised by digestion with PacI (New England Biolabs, Ipswich, UK) and ethanol precipitated before transfection ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cloned plasmids were subjected to colony PCR using Taq polymerase (New England Biolabs), restriction digestion analysis and Sanger sequencing in order to confirm the clones (Table S6 ...
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bioRxiv - Molecular Biology 2023Quote: Both HURP plasmids were transformed into Rosetta2 (DE3) Escherichia coli cells (NEB: C29871) and grown in 2 L of LB media containing 50 μg/mL ampicillin and 25 μg/mL chloramphenicol at 37 °C in a shaking incubator (200 rpm) ...
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bioRxiv - Biophysics 2023Quote: ... following the manufacturer’s protocol after plasmid linearization with NotI-HF (New England BioLabs). RNA cleanup was performed with the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Biophysics 2023Quote: ... we digested any remaining methylated wildtype plasmid using Dpn1 (New England Biolabs, R0176L) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... MBP::CDC-42 coding sequence was inserted into the pMAL-c5X plasmid (NEB). The S71A mutation was introduced into the pMALc-MBP::CDC-42 plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521 ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmids were ligated based on Gibson assembly (New England BioLabs master mix) of plasmid backbone and insert sequences ...
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bioRxiv - Molecular Biology 2023Quote: ... gRNA oligonucleotides were ligated into pX458 plasmid with T4 DNA ligase (NEB, #M0202S). The ligation reaction was transformed into DH5 α-competent E ...
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bioRxiv - Genetics 2023Quote: Plasmids for yeast two-hybrid experiments were constructed using NEBuilder DNA assembly (NEB), Ligation High kit (TOYOBO) ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmids were ligated based on Gibson assembly (New England BioLabs master mix) of plasmid backbone and insert sequences ...
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bioRxiv - Molecular Biology 2023Quote: ... Line- arized plasmid standards were obtained by digesting pAAV_GFP with HindIII (NEB, R3104S) restriction enzyme.
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bioRxiv - Evolutionary Biology 2023Quote: ... The plasmid was linearized by digestion with SapI restriction enzyme (New England Biolabs) for 1 hour in 37 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we PCR-amplified the plasmids’ inserts using Q5 polymerase (NEB, USA product #M0491) (see “Molecular Cloning” for protocol) ...
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bioRxiv - Biochemistry 2023Quote: ... the plasmid above was modified using the Q5 Site-Directed Mutagenesis Kit (NEB) along with the following primers ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cloning and plasmid propagation were performed using NEB Turbo (New England Biolabs; C2984H) competent E ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were linearized using NcoI (R0197, R3193, New England BioLabs, Waltham, MA, USA) at 37°C for 1 hr ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli were performed with the Monarch® Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... 60 μg of plasmid DNA was linearized with AhdI (New England Biolabs, UK) and extracted with phenol-chloroform for injection.
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bioRxiv - Molecular Biology 2024Quote: Plasmid DNA was purified from Escherichia coli DH5α competent cells (New England Biolabs) using a Qiagen Miniprep kit ...
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bioRxiv - Plant Biology 2024Quote: ... The pJET_ZEP3-NosT plasmid was digested with AvrII and SbfI restriction enzymes (NEB). These were then ligated with T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... gRNA-Cas9 plasmids were generated upon cleavage with BbsI restriction enzyme (NEB, R3539M) of the px330 plasmid following NEB instruction ...
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bioRxiv - Cell Biology 2024Quote: ... the plasmids were linearised by the DraI restriction enzyme (New England Biolabs; R0129), a dual cutter for the plasmid ...
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bioRxiv - Genetics 2024Quote: ... and digesting and ligating the insert into the pX458 plasmid using BbsI (NEB) and T4 Ligase (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids and PCRs were digested with the appropriate restriction enzymes (New England Biolabs), purified ...
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bioRxiv - Cell Biology 2024Quote: ... the plasmid was also treated with 1.5 µl of quick-CIP (NEB, M0525). Following digestion ...
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bioRxiv - Cell Biology 2024Quote: ... These plasmids were transformed into E.coli BL21 DE3 competent cell (New England Biolabs) and plated on agar plates containing Ampicillin or Kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... These plasmids (Table S6) in combination with the cell-free system PURExpress (NEB) were used to produce bacteriocins in vitro ...
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bioRxiv - Microbiology 2023Quote: ... the F1 to F7 plasmids were digested with the PaqCI enzyme (R0745L, NEB) and assembled in vitro using T4 ligase (M0202M ...
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bioRxiv - Immunology 2024Quote: ... plasmid and inserted with NEBuilder HiFi Assembly cloning kit (#E5520S, New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... PCR product and pSIP409 plasmid were digested with NcoI and XhoI enzymes (NEB), gel purified with Monarch DNA Gel Extraction Kit (NEB) ...
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bioRxiv - Systems Biology 2020Quote: ... rRNA depletion and library preparation for sequencing was completed with the NEBNext protocol (‘Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared according to the protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA from 1000 ng total extracted RNA was depleted using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat; New England Biolabs Inc.). The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2021Quote: DNAse-treated RNA with high RIN value was used to deplete ribosomal RNA using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6350) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and then cloned into a customized minigene plasmid (a derivative of the pSpliceExpress vector)29 containing an RSV-promoter and two control exons (rat insulin exons 2 and 3) using the NEBuilder® HiFi DNA assembly (NEB, E2621). Amplified fragments were inserted between the two control exons ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA depletion was carried on the extracted RNA using Nebnext rRNA depletion kit (Human/mouse/rat) (New England BioLabs. In, USA). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... library was prepared using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). The RNA-Seq libraries were sequenced on NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 cycles ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared from RNA samples (150ng) using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB Cat# E7405) in conjunction with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB Cat# E7765) ...
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bioRxiv - Biochemistry 2023Quote: ... An mRNA transcript library for Illumina sequencing was created using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs, Ipswich, MA). A NextSeq 500 sequencer (Illumina ...
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bioRxiv - Immunology 2023Quote: ... The other pCMVtag2B vectors containing different truncated versions of rat ITCH (Fig 2F) were generated via Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Cat#E0554S) by circularizing the PCR products amplified with the primers listed in Table S2 ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid was digested with XhoI and SacI (New England BioLabs, Ipswich, MA, USA) to remove the mutant Gsα sequence and purified via electrophoresis and gel extraction kit (Bio Basic ...
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bioRxiv - Bioengineering 2022Quote: ... Sequence verified plasmids were transformed into BL21(DE3) Escherichia coli cells (New England Biolabs) and plated on LB medium supplemented with 100 μg mL−1 ampicillin ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids encoding mouse Nav1.2 (NP_001092768.1) and Nav1.6 (NP_001070967.1) were cloned by Gibson Assembly (NEB) with synthetic gBlocks gene fragments (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested plasmid and amplified DNA library were assembled using Gibson Assembly reaction (NEB) using the insert ...
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bioRxiv - Microbiology 2019Quote: ... Insert DNA and plasmid pBA169 were digested with EcoRI and BamHI (New England Biolabs) and ligated overnight before transformation into chemically competent E ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The proA** expression plasmid was constructed using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Biophysics 2021Quote: MukF Flag-tagged fragments were expressed from pET 21 plasmids in C3013I cells (NEB).The Flag-tagged MukE and MuF were at the C-terminus ...
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bioRxiv - Microbiology 2021Quote: ... Correct plasmid sequence in isolated clonal bacteria was confirmed by both AscI/AatII (NEB) restriction digest and Sanger sequencing (University of Utah DNA Sequencing Core) ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were constructed by ligating PCR products amplified with Q5 polymerase (New England Biolabs) using Golden Gate assembly (87) ...