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Citations for New England Biolabs :
851 - 900 of 4951 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... coli in 5 x 25 uL cell aliquots (NEB #C3020). Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone ...
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bioRxiv - Synthetic Biology 2022Quote: ... Assembled products were heat-shock transformed to 5-alpha (NEB), TOP10 (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... 5 µL 10x T4 DNA ligase reaction buffer (NEB, #B0202S), 2 µL 400U/µL T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’P was created by T4 PNK treatment (NEB, M0201S), done according to the NEB T4 PNK protocol ...
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bioRxiv - Genomics 2023Quote: ... and 7.5 U of Klenow fragment 3’→5’ exo-(NEB), except for input DNA that is degraded (e.g. ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was mixed with 5 U of T4 PNK (NEB), DNA splint (0.4 pmol ...
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bioRxiv - Genetics 2023Quote: ... column-purified and transformed in 4 or 5 electroporations (NEB 10-beta Electrocompetent E ...
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bioRxiv - Plant Biology 2023Quote: ... 5% (v/v) BsaI at 20 U/μL (NEB #R3733), 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was then applied to 5 mL chitin resin (NEB) prewashed with HEGX buffer and incubated for 1 hr at 4 °C with agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 µL 10x Standard Taq Reaction Buffer (New England Biolabs) and 0.25 µL Taq-DNA polymerase (1.25 units/50 µL reaction) ...
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bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 μL of Klenow Fragment (3’ -> 5’ exo -, NEB M0212L), 5 μL 50% PEG 8000 ...
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bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... using Large Klenow fragment 3’-5’ exo- (New England Biolabs). Biotinylated 19x 601 array DNA ...
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bioRxiv - Genomics 2023Quote: ... by using Klenow Fragment (3′→5′ exo-) (New England Biolabs) for 30 min at 37 °C and purified by using a QIAquick PCR Purification Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... Linker reactions were removed with 5′ deadenylase (New England Biolabs) and Rec J Exonuclease (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The library was transformed into 5-alpha electrocompetent cells (NEB), grown in liquid culture ...
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bioRxiv - Genomics 2023Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB). The plasmid library was prepared by maxi-prep (Sigma) ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we performed 2 reactions per sample (20 µl 5× NEB Q5 buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 µl of prestained protein ladder (New England Biolabs). The gel was electrophoresed at 150 V ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of T4 PNK buffer (NEB, cat. no. M0201S) and 5 μL of 10 mM ATP (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5′-hydroxyl group repair (T4 polynucleotide kinase (M0201L; NEB) were conducted on beads ...
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bioRxiv - Developmental Biology 2023Quote: ... Protruding 5’ ends were filled in with Klenow enzyme (NEB) in the presence of [P]-32 dCTP (Hartmann Analytics ...
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bioRxiv - Molecular Biology 2024Quote: ... The 5’-phosphate was added via T4 polynucleotide kinase (NEB) at 37 ºC for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼500 bp 5’ and 3’ flanking regions of Tb427.10.12290 (NEB) with primers SMD400/1 and SMD404/5 respectively using (Lister strain 427 ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg of replicon plasmid was linearised with AscI (NEB), purified by phenol-chloroform extraction ...
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bioRxiv - Genomics 2024Quote: ... The 5′ adapter was ligated with T4 RNA ligase (NEB), followed by a third biotinylated RNA enrichment ...
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bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
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bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of 1xMNase buffer containing 60 gel units/ml (6 Kunz units/ml) of Micrococcal nuclease (NEB M0247S) were added and incubated for exactly for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles ...
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bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was passed twice at a flow rate of 0.5 mL/min through a column with 6 mL chitin beads (NEB) equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... A 6 µL mixture containing 2 µL of 40 µM Spy Cas9 NLS protein (New England Biolabs, MA, USA), 200 ng each of five sgRNAs (in 2 µL ...
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bioRxiv - Biochemistry 2021Quote: To generate circular DNA with a single nick phagemid DNA (~2,6 μg) with or without non-denaturing bisulfite treatment was treated with 20 Units of Nt.BstNBI (New England Biolabs) for 1 hour at 37°C in 50 μl of 1X NEBuffer 3.1 followed by deactivation at 70°C for twenty minutes.
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bioRxiv - Microbiology 2021Quote: ... 4°C and the supernatant applied on a 6 ml gravity flow column with Chitin resin (New England Biolabs), equilibrated with 20 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... Cx36-SNAP transfectants were treated with 6 µM SNAP-Cell Oregon Green (New England Biolabs Inc., Boston, MA, USA) in 10% FBS DMEM for 30 min at 37°C to quench all Cx36-SNAP molecules ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Microbiology 2023Quote: ... We incubated TXTL reactions at 29 °C for 6 h and digested at 37 °C with PacI (NEB R0547S) as instructed by the provider and added water instead of PacI for the undigested control ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... protein aliquots were subjected to PNGase F or a broad range mannosidase (α1,2/3/6) (New England Biolabs, France) digestion according to the manufacturer’s specifications ...
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bioRxiv - Genetics 2024Quote: ... in reactions containing 1X ThermoPol buffer (12.5mM tricine, 42.5mM KCl, 1.5mM MgCl2, 6% dextran; New England BioLabs, Ipswich, MA), 0.32 mM dNTP mix ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5× Q5 buffer and pUC19 plasmid as template (New England Biolabs) in 50 μl ...
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bioRxiv - Cell Biology 2020Quote: ... and further digested overnight with 5 U DpnII (New England Biolabs) at 37°C in a humidified atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by phosphorylation of the 5’ end with T4 kinase (NEB). Phosphorylated RNA was then purified by the RNeasy kit (Qiagen ...