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Citations for New England Biolabs :
851 - 900 of 4581 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... 2nd cDNA strand was again synthesized using a transcript specific primer (either 5’UTR_βG-intron _F or 5’UTR_CMV _F2, Table 4) and Q5 2x master mix (NEB, #M0494). After another round of bead purification ...
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bioRxiv - Systems Biology 2022Quote: ... we performed a standard PCR using all 10μl of eluted DNA with 25μl 2x NEB High-Fidelity master mix (NEB, MO544), 2.5μl of 25μM forward primer (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’) ...
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bioRxiv - Genomics 2022Quote: ... with a minimal promoter and partial 3’ UTR was amplified from pMPRAduo:minP:GFP (addgene pending) in 1600 μL volume containing 800 μL of Q5 High-Fidelity 2X Master Mix (NEB, M0492L) and 0.5 μM forward and reverse primers (primers 7 and 8 for vector A and primers 9 and 10 for vector P ...
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bioRxiv - Immunology 2022Quote: ... Two multiplex PCR tiling reactions were prepared by combining 2.5 μL cDNA with 12.5 μL Q5 High-Fidelity 2X Master Mix (NEB, USA), 6μL nuclease free water ...
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bioRxiv - Genomics 2020Quote: The 254bp V4 segment of the 16S ribosomal RNA subunit was amplified by a 17-cycle touchdown PCR using the following protocol: 12.5 μl Phusion 2X Master Mix (New England BioLabs),1 μl each of 16S V4 forward and reverse primers (primer pair 515F-806R) ...
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bioRxiv - Genomics 2020Quote: ... were added to each sample in a 9-cycle PCR reaction prepared by adding 12.5 μl Phusion 2X Master Mix (NEB) and 2 μl each of Nextera XT S5 and N7 adapters ...
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bioRxiv - Developmental Biology 2019Quote: ... Libraries were generated using 18 cycles of PCR amplification with NEB high fidelity 2x master mix (New England Biolabs), cleaned up with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA libraries were made by a limited cycle PCR Using the NEBNext High-fidelity 2X PCR Master Mix (NEB). To maximize the complexity of DNA libraries ...
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bioRxiv - Microbiology 2019Quote: Index PCR was performed with 25µL NEBNext Hig-Fidelity 2X PCR master mix (New England Biolabs Inc., Ipswich, MA), containing 5µL of each index primer (Nextera XT Index kit v2 set A ...
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bioRxiv - Cancer Biology 2019Quote: ... The purified PCR product was assembled into the linearized vector using Gibson Assembly (NEB Gibson Assembly 2x Master Mix), and transformed into E ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Purified first-round PCR products were combined with 10 μl NEBNext 2x High-Fidelity Master Mix (New England Biolabs), and 0.3 μM of each barcoding primer containing adapters and indexes to a total volume of 20 μl ...
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bioRxiv - Genetics 2019Quote: ... the MX-PCR was performed with a total reaction volume of 25 μL containing 12.5 μL Phusion Hot Start Flex 2X Master Mix (New England BioLabs), 25 ng of K562 cell line genomic DNA and 5 μL of the primer pool as defined in Supplementary Table 3 ...
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bioRxiv - Genetics 2021Quote: ... primers P1 and P2 (Table S1) were used with the Q5 Hot Start High-Fidelity 2X Master Mix (NEB). To ensure coverage for each sample ...
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bioRxiv - Microbiology 2021Quote: ... The resulting backbone was subsequently gel-purified and assembled with the PCR-amplified fragment of parBΔCTD using a 2x Gibson master mix (NEB). Gibson assembly was possible owing to a 23-bp sequence shared between the NdeI-HindIII-cut pET21b backbone and the PCR fragment ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was cleaned twice with (0.9X ...
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bioRxiv - Plant Biology 2021Quote: ... High fidelity PCR was conducted on the abcg43-1 line using the Q5 High-Fidelity 2X Master Mix (NEB). PCR products were purified and extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2020Quote: ... the targeted genomic sites were amplified by PCR with Phusion Hot Start Flew 2x Master Mix (New England BioLabs) and primers with partial Illumina sequencing adaptors ...
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bioRxiv - Genomics 2021Quote: ... 10°C hold in one cycle) by adding 25 µl of 2X PCR master mix (New England Biolabs, M0541S) and 0.8 µl of Ad 2.X reverse primer ...
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bioRxiv - Cell Biology 2020Quote: ... or OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (M0488L, New England BioLabs inc.) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... we added 1μL unique barcoded N5&N7 (0.5 µM final concentration) and 10 μL NEBNext High-Fidelity 2x PCR Master Mix (NEB). PCR cycling conditions were as follow ...
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bioRxiv - Microbiology 2022Quote: ... two multiplex PCR tiling reactions were prepared by combining 2.5 μL cDNA with 12.5 μL Q5 High-Fidelity 2X Master Mix (NEB, USA), 6μL nuclease free water ...
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bioRxiv - Genetics 2022Quote: ... All PCR reactions were conducted using Q5 High-Fidelity 2X PCR Master Mix following the manufacturer’s directions (New England Biolabs). These fragments were transformed into S ...
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bioRxiv - Molecular Biology 2020Quote: ... All PCRs for library preparation were carried out using Q5 High-Fidelity 2X Master Mix (NEB, Cat. No. M0492L). An initial enrichment amplification of 15 cycles was followed with a second round of PCR using unique P5 and P7 indexing primer combinations for 15 cycles and purified using 1.8X SPRI beads ...
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bioRxiv - Synthetic Biology 2021Quote: All PCR reactions for cloning purposes were performed using Q5® High Fidelity 2X Master Mix (New England Biolabs). The reactions mixtures were prepared using 1 ng of template ...
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bioRxiv - Genetics 2022Quote: ... PCR reactions were performed using NEBNext® High-Fidelity 2X PCR Master Mix (New England BioLabs, catalog number M0541) following the manufacture’s protocol unless specified differently ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were eluted in 20 μL of elution buffer and PCR-amplified using the NEBNext 2X Master Mix (NEB) for 11 cycles and sequenced on a NextSeq 500 (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed on 4 ng of pKD3 plasmid using Q5 High-Fidelity 2X Master Mix (New England Biolabs). The PCR product was digested for 1 hour with the restriction enzymes DpnI and ClaI at 37°C and then the PCR product was run on a 1% agarose gel ...
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bioRxiv - Microbiology 2021Quote: ... the inserts and the plasmids were mixed with Gibson master mix 2x (100 µL 5X ISO Buffer, 0.2 µL 10,000 U/mL T5 exonuclease (NEB #M0363S), 6.25 µL 2,000 U/mL Phusion HF polymerase (NEB #M0530S) ...
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bioRxiv - Bioengineering 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase or Q5 High-Fidelity 2X Master mix (New England Biolabs). PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were amplified using a one-step barcoding PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB, M0541L) and the following primers:
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bioRxiv - Molecular Biology 2019Quote: ... then amplified and indexed with unique library indices using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs). PCR products were purified with AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For molecular cloning or any other application requiring high sequence fidelity Q5 High Fidelity 2X Master Mix (NEB, M0492S) was used ...
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bioRxiv - Microbiology 2020Quote: ... Individual gBlocks fragment and a NdeI-HindIII-digested pET21b backbone were asssembled using a 2x Gibson master mix (NEB). Gibson assembly was possible due to a 23-bp sequence shared between the NdeI-HindIII-cut pET21b backbone and the gBlocks fragment ...
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bioRxiv - Synthetic Biology 2021Quote: ... The oligo was PCR amplified with OneTaq® 2X Master Mix with Standard Buffer (New England BioLabs Inc., M0482L), followed by agarose gel purification with GenCatch Advanced Gel Extraction Kit (Epoch Life Science ...
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bioRxiv - Synthetic Biology 2021Quote: ... 11.25 μ L OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.), 1.25 μ L Chai Green Dye 20X (Catalog No ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was cleaned twice with (0.9X ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated by 7 rounds of PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was used to amplify the targeted allele (Quick-Load® Taq 2X Master Mix, New England Biolabs Inc), using the following primers (at 0.4μM) ...
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bioRxiv - Immunology 2021Quote: ... Point mutations were introduced with a QuikChange site-directed mutagenesis protocol using Q5 High-Fidelity 2x Master Mix (NEB). NLRP3 mutants were named ...
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bioRxiv - Cell Biology 2020Quote: ... Integration at the correct locus was verified by colony PCR using LongAmp Taq 2X Master Mix (New England BioLabs), and the mto2 gene was sequenced in its entirety to verify the S338N or S338C point mutations.
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bioRxiv - Immunology 2021Quote: All PCR reactions were carried out in 25 μL total reaction volumes using 2X Master Mix (New England Biolabs), oligonucleotide primers synthesized by Eurofins (Ebersberg ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs encoding human elongation factor eEF2 were amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB) with primers (forward primer ...
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bioRxiv - Molecular Biology 2022Quote: ... and a PCR with 10-13 cycles was performed using the NEBNext High Fidelity 2X PCR Master Mix (NEB) and Ad1_noMX and Ad2.1–2.12 barcoded primers described in (30) ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified HTO sequencing libraries were then amplified by PCR (1μl clean HTO cDNA, 25μl 2X NEBNext Master Mix (NEB #M0541), 10 µM SI-PCR and D701or D704 primers performed dial out PCR (98°C (10 sec) ...
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bioRxiv - Biochemistry 2022Quote: ... and coding sequences were amplified and assembled into linearized pDest17 using NEBuilder Hifi Assembly 2x master mix (NEB, E2621).
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bioRxiv - Genomics 2022Quote: ... We then amplified the barcodes from each clone using Q5 High Fidelity 2X Master Mix (M0492, New England Biolabs) with primers specific to our reporter gene (CAS P58-59) ...
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bioRxiv - Genomics 2022Quote: ... Each PCR was run using 12.5μL Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA), 2.5μL template DNA ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μl of 0.1∼1 μg genomic DNA was mixed with 10 μl of 2X C-circle master mix [0.2 mg/ml BSA (NEB), 0.2% Tween-20 ...
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bioRxiv - Cancer Biology 2023Quote: ... and PCR amplified using Q5® Hot Start High-Fidelity 2X Master Mix (New England BioLabs; Ipswich, MA, USA). SafeSeqS(52 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...