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Citations for New England Biolabs :
8801 - 8850 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The R119G and T82I point mutations were added to the parent AID* construct using the Q5 Site-Directed Mutagenesis Kit (NEB, Cat # E0554S).
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using a NEBNext Poly(A) mRNA Magnetic Isolation Module and a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New Enlgand Biolabs, #E7760S/L).
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared with 1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalog #E6560L) as instructed by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... 3 ug RNA was treated with Promega RQ1 DNase (M6101) then cleaned up with the Monarch® RNA Cleanup Kit (NEB Biolabs, T2050S) and eluted in 30 µl dH2O.
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bioRxiv - Molecular Biology 2024Quote: ... The libraries were then prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs, MA, USA) in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...
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bioRxiv - Neuroscience 2024Quote: ... the sequencing libraries were generated using a NEBnext® Ultra™ II DNA library prep kit and NEBNext Multiplex Oligos (NEB #E7335) for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: Libraries were prepared using the NEBNext® DNA Library Prep Reagent Set for Illumina® kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... mCherry fluorescent protein gene was inserted into pHERD20T vector according to the manufacturing protocol of NEBuilder® HiFi DNA Assembly kit (New England Biolabs, UK). The resulting plasmid was referred to as pHERDmCh ...
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bioRxiv - Molecular Biology 2024Quote: ... EM-seq libraries were prepared with the NEBNext® Enzymatic Methyl-seq kit (E7120S) according to the manufacturer’s protocol (New England Biolabs, MA USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... 173 bp TBR-PCR target products were excised and purified using an Exo-CIP Rapid PCR Cleanup Kit (New England Biolabs, Ipswich, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to an input of 150 ng before preparation with a NEBNext® Ultra™ II RNA Library Prep (Magnetic Bead) kit (New England Biolabs). The polyA mRNA protocol was used for all preps ...
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bioRxiv - Microbiology 2024Quote: ... primers were designed to clone Ceg10 into pET28b using restriction enzymes NdeI and NotI by HiFi DNA Assemby kit (NEB; Table S4), producing the His6x-TEV-Ceg10 vector ...
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bioRxiv - Genomics 2024Quote: ... Double-stranded DNA libraries with 450 bp insert size were prepared using the ultraFS-II kit (New England Biolabs, London, United Kingdom). The QC check for the prepared libraries was done on an Agilent Tapestation (Santa Clara ...
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bioRxiv - Genomics 2024Quote: Base editor mRNAs were generated by in vitro transcription using the HiScribe T7 High-Yield RNA synthesis kit (NEB Cat No. E2040S) via the method described in48 ...
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bioRxiv - Microbiology 2024Quote: ... and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB, cat# E5520) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Complimentary DNA (cDNA) was prepared from 250 ng of total RNA using the First Strand cDNA Synthesis Kit (New England BioLabs Inc., USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... was reverse transcribed from ∼1 μg high quality RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEW ENGLAND BioLabs, Germany). Newly synthesized cDNA was purified using a the NucleoSpin® Gel and PCR Clean-up kit (Germany ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 7 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 2 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... from Col-0 cDNA and prepared for in vitro transcription with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). After purification with Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 - 400 ng of PCR fragments were used as template DNA to synthesize analytic amounts of DNA deaminases using PURExpress In Vitro Protein Synthesis kit (NEB, Ipswich, MA) following manufacturer’s recommendations.
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bioRxiv - Evolutionary Biology 2023Quote: ... The mutated enhancer sequence was synthesized as a gBlocks Gene Fragment (IDT, Coralville IA) and inserted into pattBnucGFPh using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Beverley, MA).
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bioRxiv - Biophysics 2023Quote: Purified linear and circular DNA were both in vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S). 240 ng reaction was prepared according to the manufacturer’s recommendation (see details in Supplementary Methods) ...
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bioRxiv - Microbiology 2023Quote: ... Damaged double-stranded DNA in the initial DNA sample was repaired using the PreCR® Repair Mix Kit (New England Biolabs, USA) before library construction ...
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bioRxiv - Plant Biology 2023Quote: ... RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... RNA sequencing libraries were prepared using the NEB-Next Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). The samples were sequenced on the Illumina 4000 instrument ...
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bioRxiv - Immunology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA and library preparation were performed using NEBNext® Ultra™ II Directional RNA Library Prep kit for Illumina (New England Biolabs). Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred nanograms of RNA was used to generate sequencing libraries using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... followed by stranded cDNA library generation using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs, US). All steps were performed as recommended in user manual E7760 (Version 1.0_02-2017 ...
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bioRxiv - Developmental Biology 2024Quote: Sequencing libraries were prepared using a commercial kit (NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads, New England Biolabs, Ipswich, USA), and cDNA of three replicates per strain was sequenced as 150 bp (100 bp for Mpyr_CVe13f ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genetics 2024Quote: ... The B splice form (Spc105RB) was generated using site directed mutagenesis to delete the first intron from the A form (NEB BaseChanger Kit). All mutants were generated using site specific mutagenesis of the A- or B-form Spc105R coding region in the pENTR4 vector ...
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bioRxiv - Genomics 2024Quote: ... high-molecular-weight (HMW) DNA was extracted from cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). At all steps ...
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bioRxiv - Genomics 2024Quote: ... A library for short-read sequencing was prepared using an NEBNext Ultra II DNA Library Prep kit (New England Biolabs, Beverly, MA) and sequenced using a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2024Quote: ... RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog #: E7760, New England Biolabs Inc.), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Libraries for metagenomic sequencing of CS2022-0121 and F16-31-UTM were prepared using a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) to elucidate the enrichment efficiency of the target capture sequencing ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries for regular cfDNA were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... we used a similar protocol but library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs, ref. E7370) for Illumina following manufacturer’s specifications except for end repair ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina followed by manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1pmol of each fragment were then assembled for 1h at 50°C using NEBuilder HiFi DNA Assembly master mix from the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, ref E5520S). Reaction products were amplified in provided bacteria and purified using QIAprep Spin Miniprep Kit.
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bioRxiv - Molecular Biology 2024Quote: ... Enriched DNA was subjected to library construction using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (catalog no. E7645L, NEB), with the adapter ligation step performed at 50°C instead of 65°C ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, catalog #E6560S) with oligo-dT priming according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-Seq libraries are prepared by Integragen SA (Evry, France) with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina according to supplier recommendations (NEB). The capture is then performed on cDNA libraries with the Twist Human Core Exome +Custom IntegraGen Enrichment System according to supplier recommendations (Twist Bioscience) ...