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Citations for New England Biolabs :
8651 - 8700 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 1-3 μg RNA was used to generate sequencing libraries with NEBNext Ultra™ RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) with poly(A ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg total RNA with RIN value above 7 was used for library preparation by Genewiz using NEBNext® UltraTM RNA Library Prep Kit for Illumina® and NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen) ...
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bioRxiv - Plant Biology 2021Quote: ... Library concentration was further quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England BioLabs®, Inc.). Libraries were sequenced on one lane of the Illumina® HiSeq4000 platform to generate 150-bp paired-end reads.
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bioRxiv - Molecular Biology 2020Quote: ... sgRNA target site mutation in mouse Thap1 cDNA was generated by Q5 Site-Directed Mutagenesis Kit (NEB, see Table S3 for primers). WT or Thap1-/-Brca1Δ11 MEFs (1 × 106 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries for sequencing were prepared using NEBnext Ultra II directional RNA library prep kit for Illumina (New England Biolabs, Cat. No E7760L). Libraries were sequenced on an Illumina HiSeq 1500 instrument at the Laboratory of Functional Genomic Analysis (LAFUGA ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries were generated using 10ng purified ChIP-DNA and the NEBNext®Ultra IIDNALibrary Prep Kit for Illumina® (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext Ultra II FS DNA library kit for Illumina with 384-unique indexes (New England Biolabs, Massachusetts, USA; Cat. No: E6617L). Libraries were sequenced using the HiSeq X10 with 150 bp paired-end chemistry (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... while libraries for Illumina sequencing were prepared by using a NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The synthesized oligo pool of CRISPRa library was PCR amplified with primers (table S5) including the BsmBI recognition sites using Phusion®High-Fidelity PCR Kit (NEB, E0553L). After purification with DNA Clean & Concentrator-25 (Zymo Research Corporation ...
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bioRxiv - Microbiology 2021Quote: ... resulting in ∼1 ml of turbid bacterial culture which was subsequently used as input for plasmid purification by miniprep kit (New England Biolabs, cat#T1010S). The pooled plasmid library DNA for each library was eluted in 35 μl of elution buffer and quantified ...
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bioRxiv - Microbiology 2021Quote: ... and ∼ 90 ng was used for one-step RT-qPCR using Luna® Universal one-step RT-qPCR kit (New England BioLabs Inc.) [10 min ...
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bioRxiv - Immunology 2021Quote: ... The linearized vector and the synthesized fragment in a 1:2 molar ratio were assembled with the NEBuilder HiFi DNA Assembly Kit (NEB, Ipswich, USA) at 50°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: A total amount of 0.4 ug of RNA was used for cDNA library construction at Novogene using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (cat NEB #E7775 ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNAs were reverse transcribed to cDNA using ProtoScript II reverse transcriptase with oligo dT and random primers (First Strand cDNA Synthesis kit New England Biolabs, Ipswich, MA). RT-PCRs specific for the Cas9 or actin gene of B ...
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bioRxiv - Microbiology 2020Quote: ... and one fragment of about 320 bp of the 3’-terminal region by 3’ RACE were amplified using Phusion High-Fidelity PCR Kit (New England Biolabs, MA, USA) under the following conditions [98°C ...
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bioRxiv - Plant Biology 2021Quote: ... mRNA was enriched with oligo(dT)-beads and cDNA was synthesized with random hexamer primers with the NEB Next Ultra RNA Library Prep Kit (NEB, Ipswich, USA). All libraries were sequenced using the Illumina NovaSeq6000 platform in paired-end mode with a read length of 150 bp.
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bioRxiv - Microbiology 2020Quote: ... The products of this reaction were prepared for Illumina sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed in a two-step manner using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, Cat#E6300S) and iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting single stranded cDNA was further In-vitro transcribed (IVT) using HiScribe T7 Quick High Yield RNA Synthesis Kit according to the manufacturer’s protocol (New England BioLabs Inc, NEB #E2050). The amplified RNA was than purified using Monarch RNA Cleanup Kit (New England BioLabs Inc. ...
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bioRxiv - Microbiology 2021Quote: ... samples were harvested and RNA was isolated and reverse trancripted in cDNA following protocol manufacturer (LunaScript™RTSuperMix Kit, New England Biolabs Inc). The 20 μL reaction consisted in 4 μL of RT Mix completed with 16 μL of water with 100 ng of RNA ...
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bioRxiv - Microbiology 2021Quote: ... Samples were treated with chloroform and the aqueous phase was used in the Monarch® RNA Cleanup Kit (New England Biolabs Inc).
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bioRxiv - Cell Biology 2020Quote: ... of full-length SEC16A were generated by mutating 10 nucleotides in the silencer-select Sec16A siRNA (5’-CGGAGCUUCUGUUACGAGATT-3’, siRNA id: s19236, Thermo-Fischer Scientific) binding site using Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... pBGC was constructed by joining the amplified pBGT backbone and the gfp terminator region using the Gibson Assembly Cloning Kit (New England BioLabs, MA, USA). Resulting reaction was transformed by heat shock into NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2020Quote: ... we prepared a fragmented DNA library using a NEBNext Ultra II FS DNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Sequencing of a paired-end 2 × 150 bp mode on a HiSeq X system (Illumina ...
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bioRxiv - Immunology 2019Quote: Plate based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs Inc, E6420L). Cells from donor 364b were snap frozen in 10% DMSO in 90% BSA ...
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bioRxiv - Microbiology 2021Quote: ... were prepared using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina modules E7810L and E7595L (New England BioLabs) following the manufacturer protocol for use with inputs ≥ 100 ng with modifications to eliminate PCR-amplification steps.
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bioRxiv - Immunology 2021Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: The DNA library was prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions and sequenced using the Illumina MiSeq platform with a read length of 2 × 150 bp (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... <200nt isolates were immediately frozen at -80⍰°C while >200nt isolates were used as template for cDNA synthesis (LunaScript RT Mastermix Kit, New England Biolabs, MA, USA) and subsequent doublesex sexing PCR as previously described (25) ...
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bioRxiv - Microbiology 2022Quote: ... The rRNA-depleted total RNA was subjected to library preparation using NEBNext® Ultra™ II RNA Library Prep Kit (E7700, NEB) and barcoded with NEBNext Multiplex Oligos for Illumina (E7730 ...
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bioRxiv - Microbiology 2022Quote: ... All capped viral RNA was in vitro transcribed from the corresponding linearized plasmid using the HiScribe T7 antireverse cap analog (ARCA) mRNA kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... total RNA libraries were built using the NEBNext Ultra II library prep kit for Illumina (New England BioLabs inc., product code: E7775) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... the plasmid library was linearized by BsmbI digestion and ligated with TmU fragments with Quick ligation™ kit (New England BioLabs, M2200S) for 5 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: Poly-A libraries (figure 1 and related data) were constructed with the NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs, Ipswich, USA), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA integrity was validated by gel electrophoresis and 800 ng were reverse transcribed using the LunaScript® RT SuperMix Kit according to manufacturer’s instructions (E3010, New England Biolabs, Ipswich, MA). Real-time PCR was performed using the Blue ŚGreen qPCR Kit (F416 ...
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bioRxiv - Genetics 2022Quote: ... The tRNAs were re-phosphorylated and converted to small RNA sequencing libraries using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs). Size selection of 140-155 bp PCR amplified fragments (corresponding to 19-35 nt tRNA fragments size range ...
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bioRxiv - Physiology 2022Quote: ... 3-10 ng of immunoprecipitated DNA was used to generate adapter-ligated DNA libraries using the NEBNext® Ultra DNA library kit for Illumina (New England Biolabs, E7370L) and indexed multiplex primers for Illumina sequencing (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... 100bp strand-specific whole transcriptome libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Herfordshire, UK), following rRNA depletion with NEBNext rRNA Depletion Kit or Ribo-Zero Gold ...
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bioRxiv - Cell Biology 2022Quote: ... mutated FN constructs were PCR amplified and ligated into BamH1+Xho1 cut NSPI vector using NEBuilder® HiFi DNA Assembly kit (BioLabs, #E5520).
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bioRxiv - Genomics 2022Quote: ... wild-type and mutant NEAT1 RNA fragments were transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S) and labelled with Biotin using Biotin RNA Labelling Mix (Roche ...
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bioRxiv - Synthetic Biology 2019Quote: ... The three backbone fragments were combined together with the synthesized CggR cis-regulatory element using the NEB Gibson assembly kit (New England Biolabs, MA, US) according to the manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... sequencing libraries were constructed with the NEBNext® Ultra™RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, USA), and Illumina PE (150 bp x 2 ...
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bioRxiv - Developmental Biology 2019Quote: ... pOI191 was derived from this mScarlet clone (pOI186) by mutating a single nucleotide to convert mScarlet to mScarlet-I using the Q5 Site-Directed Mutagenesis kit (NEB Cat#E0554). Primers used for cloning:
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bioRxiv - Plant Biology 2019Quote: ... Illumina paired-end libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA), before being sequenced using Illumina HiSeq 2500 platform (Hokkaido System Co ...
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bioRxiv - Molecular Biology 2020Quote: Thermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). Thermocycler was adjusted for heteroduplex formation as following ...
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bioRxiv - Microbiology 2019Quote: ... each of the amplicons were diluted to 0.5 nM and amplified using barcoding primers (1D PCR Barcoding Amplicon Kit, Oxford Nanopore Technologies, [ONT], UK) and LongAmp Taq 2X Master Mix (New England Biolabs, MA, USA) with the following conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... and then performed library preparation using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA), according to the manufacturer’s instructions ...