Labshake search
Citations for New England Biolabs :
8601 - 8650 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... Libraries were made using polyadenylated mRNA isolation and employing NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Three samples had lower RNA amounts and only a higher efficiency bead capture was employed for those ...
-
bioRxiv - Biochemistry 2020Quote: ... Mutations were introduced into the ATP11A ORF using a The Q5® Sit-direct mutagenesis kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2020Quote: ... Up to 10 ng of cDNA was used for the Illumina sequencing library construction using NEBNext® Ultra™ DNA Library Prep Kit (NEB). Paired ends sequencing was performed on NextSeq 500 (Illumina ...
-
bioRxiv - Physiology 2019Quote: ... Systematic promotor truncations were generated using the Q5 Site-Directed Mutagenesis Kit according to manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Plasmid constructs were introduced via Neon electroporation (Invitrogen ...
-
bioRxiv - Cancer Biology 2020Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
-
bioRxiv - Genomics 2020Quote: ... A cDNA library with insert sizes of 350-400 bp was prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, MA, USA) and paired-end sequenced on the HiSeq X ten platform (Illumina Inc. ...
-
bioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized from extracted RNA using the ProtoScript First Strand cDNA Synthesis Kit (#E6300, New England Biolabs Inc, Beverly, MA, USA). CDKN1A expression levels were measured using quantitative PCR (qPCR ...
-
bioRxiv - Microbiology 2019Quote: ... Measurement of differential gene expression was conducted using a qTower 2.2 (Analytik Jena, Jena, Germany) with the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswitch, USA). Primer pairs used for the analysis are listed in Table S3 D ...
-
bioRxiv - Microbiology 2019Quote: ... truncation mutations in both bacterial and mammalian expression vectors were generated using the NEB Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA). All constructs were sequence-verified using universal primers specific to the construct backbone (GENEWIZ ...
-
bioRxiv - Plant Biology 2019Quote: ... The fragmented gDNA was used for the generation of libraries with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, MA, USA). We used different combinations of primers as barcodes to pool two different libraries into one SMRT cell ...
-
bioRxiv - Genomics 2019Quote: ... expressing ATG7 isoform 2 was a gift from Toren Finkel (Addgene plasmid #24921).31 The plasmid pCMV-myc-Atg7(1) expressing ATG7 isoform 1 was derived from pCMV-myc-Atg7(2) using the Q5 site-directed mutagenesis kit (NEB, Ipswich, MA). Subsequently ...
-
bioRxiv - Neuroscience 2019Quote: ... and libraries were constructed in technical triplicate using NEBNext Poly(A) Magnetic Isolation Module and NEBNext UltraII RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA), with size selection by AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Biochemistry 2019Quote: ... The gene was cloned into the first open reading frame of a pCDF-Duet vector using Gibson Assembly Kit (NEB, Ipswich, MA).[23 ...
-
bioRxiv - Genomics 2019Quote: RNA libaries were prepared using both E7530L and E&335L NEBNext® Ultra™ RNA Library Prep Kits (New England Biolabs, USA) to generate fragment sizes of 351-371 bp ...
-
bioRxiv - Genomics 2021Quote: ... One microgram of mRNA was used as a template for first-strand cDNA synthesis using NEBNext® Poly(A) kit (NEB #E7490, New England Biolabs, Inc.) and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760 ...
-
bioRxiv - Microbiology 2021Quote: ... This PCR product was cloned into pBHM2403digested with NotI and SacII by Gibson assembly using the NEB Hifi DNA Assembly Kit (New England Biolabs, Ipswich, MA). To generate a sgRNA expression cassette ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and ligated with Illumina sequencing adapters using a NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs, Ipswich MA, USA), and further PCR enriched with primers of P5 and P7 oligos ...
-
bioRxiv - Cell Biology 2021Quote: ... Libraries were prepared using a NEBNext Ultra RNA Library Prep Kit and NEB Next Multiplex Oligos (New England Biolabs E7530L and E7335L). Library quality and size distribution was confirmed by running an Agilent High Sensitivity DNA assay (Agilent Genomics 5067-4626) ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNA library was constructed using the NEBNext® UltraTM RNA library preparation kit as per the manufacturers’ protocol (New England BioLabs, USA). mRNA was first purified via the addition of poly-T-oligo-attached magnetic beads ...
-
bioRxiv - Genomics 2021Quote: ... Strand-specific RNA-seq libraries were constructed using the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs). The libraries with adapters were sequenced on the Novaseq 6000 (Illumina ...
-
bioRxiv - Molecular Biology 2020Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
-
bioRxiv - Plant Biology 2020Quote: ... Transcript knockdown was confirmed in T0 and T1 plants using qPCR (qScript cDNA synthesis kit, QuantaBio®, and Luna qPCR mix; NEB®) according to manufactuers’ protocols ...
-
bioRxiv - Developmental Biology 2021Quote: ... sgRNA synthesis was performed by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
-
bioRxiv - Biophysics 2021Quote: ... The vimentin constructs (Y117L mutants and addition of a Spot tag) were produced using a Q5 Site-Directed Mutagenesis kit (Biolabs cat # E0554S) using the primer lists in Table 1 ...
-
bioRxiv - Microbiology 2021Quote: ... mixtures for the detection of viral RNA were made by a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs, USA) in accordance with the manufacturer’s instructions and then placed in a thermocycler (BIO-RAD CFX96™ Real-Time System ...
-
bioRxiv - Microbiology 2022Quote: ... and ligated with Illumina sequencing adapters using a NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs, Ipswich MA, USA), and further PCR enriched with primers of P5 and P7 oligos ...
-
bioRxiv - Cell Biology 2021Quote: ... A232E site-directed mutation in RFP-VCP was done with a Q5 site-directed mutagenesis kit (New England Biolabs Japan, Tokyo, Japan).
-
bioRxiv - Cell Biology 2021Quote: ... The beads were then re-suspended in 23 μL of 10 mM Tris-Cl pH 8.0 and libraries were prepared by on-bead reactions using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand and the supernatant was discarded ...
-
bioRxiv - Neuroscience 2021Quote: ... The libraries were prepared from total RNA enriched for mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Preparation Kit for Illumina (New England Biolabs, MA, USA) and sequenced on an Illumina NextSeq 500 instrument (Illumina ...
-
bioRxiv - Genomics 2020Quote: ... and PCR enrichment of adapter-ligated DNA was performed for 10 cycles using NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs). Amplified libraries were purified with 0.7x SPRIselect beads and sequenced with MiSeq Reagent Kit v3 ...
-
bioRxiv - Genomics 2021Quote: ... NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® according to the manufacturer’s instructions (New England Biolabs).
-
bioRxiv - Neuroscience 2021Quote: ... Strand-specific RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) after poly(A ...
-
bioRxiv - Cancer Biology 2020Quote: cDNA was synthesized from 500 ng of each respective RNA sample using the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, E6300L) according to manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... strand-specific RNA-seq libraries were constructed by using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... 3 to 15 ng of immunoprecipitated DNA were used to prepare the sequencing libraries using the NEBNext Ultra II DNA Library Prep Kit (NEB, Ref: E7645S) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 single guide RNA templates fused to a scaffold were synthesized for each target gene using HiScribe™ SP6 RNA Synthesis Kit (New England BioLabs, E2070S). Injection mixture was prepared on ice containing 5 µM Cas9 (PNA Bio ...
-
bioRxiv - Cell Biology 2021Quote: ... Purified RNA was submitted to the University of Virginia Genome Analysis and Technology Core for whole transcriptome sequencing by first library preparation with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB Cat #E7760) and then sequencing by Illumina NextSeq 500 Sequencing System for paired-end 75bp reads ...
-
bioRxiv - Genetics 2021Quote: ... DNA libraries were prepared using 300 ng of fragmented DNA following the instructions of the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs). Samples were sequenced on a HiSeq 2500 (Illumina ...
-
bioRxiv - Microbiology 2021Quote: ... Libraries were prepared for sequencing by Cambridge Genomics Services using 3 μg of total RNA and an Ultra™ RNA library prep kit for Illumina (NEB, USA). Libraries were quantified by PCR ...
-
bioRxiv - Genomics 2020Quote: ... Approximately 1 µg of fragmented DNA was used for each library preparation using the NEBNext® DNA Library Prep kit (New England Biolabs, UK); DNA-libraries were multiplexed with NEBNext Multiplex Oligos for Illumina kit (New England Biolabs ...
-
bioRxiv - Genomics 2020Quote: ... and once the solution is clear 48 uL of the elution was transferred to a 2mL Lo-Bind tube with End-Repair and A-tail buffer (7 uL) and enzymes (3 uL) premixed (NEBNext® Ultra™ II kit, New England Biolabs). The solution was then mixed by gentle tapping and transferred to a 0.2 mL PCR tube and incubated at 20 degrees C for 30 minutes and 65 degrees C for 30 minutes in a thermocycler ...
-
bioRxiv - Genomics 2019Quote: ... The available CPQ cDNA was previously prepared from total RNA derived from midguts of fifth-instar larvae fed on semi-synthetic diet using a Protoscript II kit (NEB, Ipswich, MA). PCR was carried out on both strains using primers designed to flank the region of the kdr mutation in the para gene (Forward 5’-ACCAAGGTGGAACTTCACAGAT −3’ Reverse 5’-AGCAATTTCAAGAAGTCAGCAACA −3’) ...
-
bioRxiv - Neuroscience 2020Quote: ... as well as wildtype (WT) N2aC were assayed for mis-matched DNA pairs via T7 endonuclease assay (EnGen Mutation Detection Kit; New England Biolabs, Ipswich, MA) with PCR primers targeted towards our genomic region of interest (Integrated DNA technologies ...
-
bioRxiv - Microbiology 2021Quote: ... The first EcoRI restriction site at 829 bp within the AgeI and KasI restriction sites in RGD4C-AAVP-TNF was deleted in two steps to mutate a thymidine to cytosine nucleotide at position 833 without altering the translated amino acid using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... RNA (25-100 ng) was mixed with random oligonucleotide primers using the NEBNext® First Strand Synthesis Module kit for Illumina® (NEB) and incubated for 10 min at 94°C ...
-
bioRxiv - Immunology 2020Quote: ... Two hundred nanograms of total RNA was subsequently processed to generate RNA-seq libraries using NEBNext Poly(A) mRNA Magnetic Isolation and Ultra II Directional RNA Library Prep kit for Illumina (NEB E7490, E7760) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Preparation of a small RNA-Seq library from submitted total RNA sample was performed using the NEBNext® small RNA library preparation kit (New England Biolabs, UK). Small-RNA sequencing was conducted using the Illumina HiSeq 4000 (Illumina ...
-
bioRxiv - Immunology 2020Quote: ... sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs, Ipswich, MA, USA). Purified sgRNA (0.5 μg ...
-
bioRxiv - Molecular Biology 2020Quote: Primers for generating mutations in hTHAP9 and DmTNP coding sequences were designed using NEB Base changer tool™ and site directed mutagenesis was performed using the Q5 Site directed Mutagenesis kit (NEB E0554S) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: The DNA for the gene-of-interest with overlap extensions were ligated into the digested vectors using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB # E5520S) by incubating at 50 °C for one hour according to the manufacturer’s instructions ...