Labshake search
Citations for New England Biolabs :
8451 - 8500 of 10000+ citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... The final Golden Gate reaction volume contained 1× concentrated T4 ligase buffer (NEB), 250 ng Golden Gate vector (pcDNA3-based carrying a twin Strep-FLAG tag ...
-
bioRxiv - Genetics 2023Quote: ... the rRNA was eliminated by adding 1 μl RNase H (New England Biolabs), 4 μl 10X RNase H buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.5% Triton X-100) supplemented with Proteinase K (1:100, New England Biolabs). Specimens were incubated on an orbital shaker at 37°C per 2 hours and 60 rpm.
-
bioRxiv - Biophysics 2023Quote: ... The left lane (“L”) has a DNA size ladder (1 kb+, NE Biolabs). The black arrow in the left lane (“P” ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) by NEB following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB, #E7600S) with primer indexing PCR run according to the Epicypher protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2 μl were combined with 1 μl 10X T4 Ligation Buffer (NEB, M0202), 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK ...
-
bioRxiv - Microbiology 2023Quote: ... RNA concentration was determined by nanodrop and DNase 1 treated (NEB, Cat. # M0303L). DNase 1 (4 units ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1μg RNA was incubated with either 1 unit of XRN1 (New England Biolabs) or water for 2hr at 37°C ...
-
bioRxiv - Bioengineering 2023Quote: ... 4 ng gBlock template and 1 Unit Phusion High-Fidelity DNA polymerase (NEB). The reaction was subjected to a 2-minute initial denaturation at 98 °C ...
-
bioRxiv - Developmental Biology 2023Quote: ... were ligated using NEBNext DNA Sample Prep Reagent Set 1 (New England Biolabs). Immunoprecipitations were carried out using 500ng DNA per sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 μl of 10x T4 RNA ligase 2 (truncated) buffer (New England Biolabs), 3 μl of 50% PEG 8,000 (New England Biolabs) ...
-
bioRxiv - Biochemistry 2023Quote: ... Oligo 1 and oligo 2 were annealed by T4 polynucleotide kinase (NEB, #M0201S) at 37 ℃ for 30 min and 95 ℃ for 5 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... sgCD81i-1 g[9nt] and g[8nt] guides required golden gate assembly (NEB) due to the short length of the oligonucleotides ...
-
bioRxiv - Genomics 2023Quote: ... Nuclei were then digested with DpnII (1:50) (NEB, 50 000 U/ml) for 4h at 37°C under agitation ...
-
bioRxiv - Genomics 2023Quote: ... 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs) and 700 µL of PCR-grade H2O ...
-
bioRxiv - Molecular Biology 2023Quote: ... 39 µL of qPCR mix containing 1× Phusion mix (New England Biolabs, M0531S), 0.125 µM P6 Tall v4 primer ...
-
bioRxiv - Genetics 2023Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the YCp50-WT_PKR template ...
-
bioRxiv - Genomics 2023Quote: ... were added to the reaction with T4 DNA Polymerase (1 unit; NEB, M0203) and incubated at 12°C for 15 min ...
-
bioRxiv - Genomics 2023Quote: ... Beads were re-suspended in 30μl of 1 × NEB Buffer2 (NEB Cat#7002S) containing 9U of T4 DNA polymerase (NEB ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and treated with 1 µL (20 units) of DpnI (New England Biolabs, R0176L) for 1 h at 37°C before being purified on magnetic beads ...
-
bioRxiv - Microbiology 2023Quote: ... followed by incubation for 1 hr with 4 units of DNase I (NEB) at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 100 ng/µL injection mix (70 ng/µL 1 kb DNA ladder (NEB), 30 ng/µL plasmid of interest ...
-
bioRxiv - Systems Biology 2023Quote: ... primers of the NEBNext dual primer set 1 (New England Biolabs, Ipswitch, MA) were used ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 founder with the L424V was determined by restriction digest with MseI (NEB) and Sanger sequence analysis ...
-
bioRxiv - Cell Biology 2024Quote: ... then 1 μL Endo H and 2.5 μL GlycoBuffer 3 (New England Biolabs) was added and incubated for 1 hour at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... and 1/10th volume of DNAse I reaction buffer (NEB cat. no. B0303S) were added to 1 mL of phage lysate and incubated at 37°C for 30 min followed by a 60 min incubation at 65°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mM phosphoenolpyruvate and 0.15 mg/ml bovine serum albumin (New England Biolabs), in a final volume of 15 µl ...
-
bioRxiv - Systems Biology 2024Quote: ... then digested for 1 hour at 37 °C using SacI (New England Biolabs). This linearized plasmid was used for Gibson assembly with an insert composed of a 248 base pair region of PBS2 amplified from genomic DNA ...
-
bioRxiv - Genetics 2024Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the MSp509_PKR-WT template DNA ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 µl of tailing mix (0.05 µl Terminal Transferase (NEB, cat. no. M0315), 0.1 µl 10x Cutsmart buffer ...
-
bioRxiv - Microbiology 2020Quote: ... The cDNA libraries were then constructed from 50 ng of depleted RNA using the NEBNext Ultra II Directional RNA library prep kit following manufacturer instructions (NEB). Libraries were size-selected to 200-700 bp using SPRIselect beads and quality was assessed on the Agilent Bioanalyzer using High Sensitivity DNA chips ...
-
bioRxiv - Microbiology 2020Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ DNA Library Prep Kit (New England BioLabs Inc., Ipswich, MA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting fragment was inserted into the HindIII-BamHI sites of pJB866 by an NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The nucleotide sequence of the insert was confirmed by sequencing ...
-
bioRxiv - Cancer Biology 2021Quote: ... mRNA Magnetic Isolation Module and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs). Library quality was checked on an Agilent TapeStation 4150 and quantified by Qubit 2000 fluorometer (Invitrogen) ...
-
bioRxiv - Cancer Biology 2021Quote: ... mRNA Magnetic Isolation Module and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs). Library quality was checked on an Agilent TapeStation 4150 and quantified by Qubit 2000 fluorometer (Invitrogen) ...
-
bioRxiv - Biophysics 2021Quote: ... Indel frequencies at the SaCas9 target site were assessed via a T7E1 assay with the EnGen Mutation Detection Kit (NEB), using the manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2021Quote: ... The AcrIIA4 expression vector was modified to express AcrIIA11 with a C-terminal NLS and HA tags using the HiFi Assembly Kit (NEB).
-
bioRxiv - Neuroscience 2021Quote: ... were ligated to end-repaired and A-tailed chromatin using components from NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) (25).
-
bioRxiv - Cancer Biology 2021Quote: ... RNA was subsequently sent for either library preparation and RNA-sequencing to Genewiz or cDNA preparation via a two-step process beginning with Lunascript RT Supermix Kit (#E3010, New England Biolabs). qRT-PCR was performed on cDNA from samples for genes of interest using the Luna Universal qPCR Master Mix (#M3003 ...
-
bioRxiv - Cancer Biology 2021Quote: ... phosphorylated and bead-purified in preparation for A-tailing using a custom NEB Paired-End Sample Prep Premix Kit (New England Biolabs). Illumina sequencing adapters were ligated for 15 minutes at 20°C and adapter-ligated products were then bead-purified and enriched with 10 cycles of PCR using primers containing a hexamer index that enables library pooling.
-
bioRxiv - Cancer Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and purified using the Monarch Gel Extraction kit (New England Biolabs). Purified PCR products were Sanger sequenced by Genewiz using the BPNT1_F_Seq primer ...
-
bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated as described above and sample preparation was performed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420S/L; NEB) according to the protocol ...
-
ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... RNA-Seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs # E7420S) by following the company's protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed at the Center for Health Genomics and Informatics using the NEBNext Ultra II Directional RNA Library prep kit (New England Biolabs) with ribosomal RNA depletion ...
-
bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
-
bioRxiv - Genomics 2020Quote: ... The DNA was sheared to 650 bp using Covaris S220 and subsequently used for DNA-seq library preparation with NEBNext Ultra DNA Library Prep Kit (New England Biolabs). The paired-end sequencing datasets with 101 nt read length (PE101 ...
-
bioRxiv - Cell Biology 2019Quote: ... One μg of purified RNA was reverse transcribed into cDNA using the Protoscript II Reverse Transcriptase kit (New England Biolabs). Quantitative real time polymerase chain reactions (qRT-PCR ...
-
bioRxiv - Cell Biology 2020Quote: ... The PCR fragments were cloned into the linearized pUC19 vector using the Gibson Assembly Cloning Kit (E5510S; New England Biolabs).
-
bioRxiv - Cell Biology 2020Quote: Site-directed mutagenesis of NES and hydrophobic-stretch coding sequences was performed using the Q5 site-directed mutagenesis kit (New England Biolabs) with either pCPLJJ50 ...