Labshake search
Citations for New England Biolabs :
8201 - 8250 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: All clonings were carried on in a Gibson assembly-like reaction with the NEBuilder® HiFi DNA Assembly kit (NEB, E2621) and into plasmids derived from pMM704 or pMM731 (17) ...
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bioRxiv - Bioengineering 2023Quote: mRNA was synthesized through in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, China, Cat: E2080S). The templates for in vitro transcription were linearized plasmids ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA libraries from at least three replicate individuals per sex for each lineage (Table S1) were prepared from gametes using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs). Mature gametophytes release gametes just after the medium renewal ...
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bioRxiv - Molecular Biology 2023Quote: ... A PCR-amplified DNA fragment of pcDNA3-DNMT1 was generated using Q5 Site-Directed Mutagenesis Kit (E0554S, NEB, Ipswich, MA, USA). The primers used in this process are described in Supporting Information ...
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bioRxiv - Microbiology 2022Quote: ... Residues that failed this method were substituted by re-cloning the gene as two overlapping fragments using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB) according to supplier protocol and recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... The APOBEC3A and BSA materials used were purchased from the NEBNext© Enzymatic Methyl-seq Conversion Module kit from New England Biolabs (NEB). The reaction volume was then scaled down to 15.5 uL and modified from the original deamination protocol from the kit ...
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bioRxiv - Biochemistry 2023Quote: ... The mutant and wild-type target RNA were subsequently amplified using either the NEB Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB E3019), Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019 ...
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bioRxiv - Immunology 2023Quote: ... 250 ng of input DNA was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645S) and sequencing adaptors were ligated using NEBNext Multiplex Oligos for Illumina (#E7500S ...
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bioRxiv - Bioengineering 2023Quote: ... and a gBlock (purchased from Integrated DNA Technologies [IDT]) containing an anhydrotetracycline (aTc) inducible promoter (pLTetO-1) were inserted via HiFi Assembly (HiFi Assembly Kit (NEB#E5520S)) into a PCR-ed fragment of plasmid pBbA2c-RFP (Addgene #35326 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Developmental Biology 2022Quote: 50mg of muscle tissue were obtained from a euthanized adult female sugar glider and high-molecular weight DNA was extracted using the Qiagen MagAttract HMW kit (NEB 67563). DNA was quantified using the Qubit DNA HS kit (Thermofisher Q32851 ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared by the Cornell TREx facility using NEBNext Ultra II Directional Library Prep Kit (New England Biolabs, Ipswich, MA) with ribosomal RNA depletion ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We prepared genomic DNA libraries for the 16 samples using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Inc). We quantified the quality of the genomic DNA library using the Agilent 2100 Bioanalyzer system located at the Genome Analysis Core Laboratories at Georgia Institute of Technology (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... RNA-seq library preparation was performed with the NEBNext Ultra II RNA Library Prep Kit and sequenced with the Illumina platform (NEB E7770S). Ribosomal RNAs (rRNAs ...
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bioRxiv - Cancer Biology 2023Quote: ... and sequencing libraries were prepared from 5 ng of the purified amplicon using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, E7370L) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... MNase activity was stopped and chromatin fragments released into supernatant were purified using the NEB Monarch DNA Cleanup Kit (NEB, T1030) per manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: DNA from in vitro and in vivo experiments was sent to Novogene (Sacramento, CA) and library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... while Icelandic and eastern Greenland rock ptarmigan DNA was extracted using Monach™ Genomic DNA Purification Kit (New England BioLabs, USA). DNA was checked for concentration and purity using a NanoDrop® 2000 spectrophotometer and Qubit® 3.0 fluorometer Quantitation Kit (Invitrogen™) ...
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bioRxiv - Genomics 2022Quote: ... mScarlet and HPRT (housekeeping gene for normalization) levels were then measured by qPCR using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005) with 100ng total RNA per reaction ...
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bioRxiv - Genomics 2022Quote: The extracted genomic DNA was used for library construction using NEBNext® DNA Library Prep Kit (New England Biolabs, MA, USA), and sequencing (150 bp paired end ...
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bioRxiv - Genetics 2023Quote: ... Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB, #E7645S). Sequencing was performed on an Illumina HiSeq 3000 ...
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bioRxiv - Genetics 2023Quote: ... Subtraction of poly-A RNA using NEBNext Ultra II with a Poly-A Selection kit (New England Biolabs, Ipswich, MA, USA) and poly-A RNA sequencing were performed at Admera Health (South Plainfield ...
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bioRxiv - Genetics 2022Quote: ... Libraries were prepared from 500ng of the total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) with Poly-A mRNA magnetic isolation (NEB #E7490 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The purified product was used as template for dsRNA synthesis using HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) with subsequent purification with MEGAclear™ Transcription Clean-Up Kit (Ambion) ...
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bioRxiv - Genetics 2023Quote: ... Then the sequencing library was generated by using the NEBNext® Ultra II DNA Library Prep Kit (New England Biolabs; E7103S) and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... using Custom Nextera PCR primer and the NEBNext High-Fidelity 2x PCR Master Mix (Nextera DNA Library Preparation Kit (New England BioLabs, E7530L) and purified with the Qiagen MinElute Kit (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... pCS2-dKif6 (1-501)-EGFP was generated from the pCS2-dKif6-EGFP plasmid by Gibson assembly (NEB HiFi DNA Assembly Kit) using a GeneArt gene fragment encoding amino acids 1-501 (Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... Three independent sequencing libraries for each genotype were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). Illumina NovaSeq 6000 was used for sequencing ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: 500 ng of RNA was processed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB) with prior enrichment of mRNA by using Oligo dT beads from the NEBNext Poly(A ...
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bioRxiv - Genomics 2023Quote: ... E7490) and NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Inc., Cat. No. E7420). All libraries were dual-indexed using 12 cycles of PCR amplification using NEBNext® Multiplex Oligos for Illumina® Dual Index Primer Set (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... DNA was used to prepare a short reads library using NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs, England) and sequenced on Illumina HiSeq X instrument (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... 125 ng of fragmented DNA was used to prepare each DNA library following the NEBNext Ultra II library prep kit for Illumina (NEB, Inc.) until the loop-adaptor ligation step ...
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bioRxiv - Biophysics 2023Quote: ... PUS1D134A lacking the N-terminal HIS-tag was subcloned into expression vector pET21d (EMD Biosciences) using a Gibson Assembly cloning kit and protocol (NEB # E5510S) and sequence verified ...
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bioRxiv - Developmental Biology 2023Quote: ... was combined with either MEP-1 or Mi-2 PCR-amplified coding sequences in a Gibson assembly reaction using NEBuilder® HiFi DNA Assembly kit (NEB) following manufacturers protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... All mRNAs were transcribed at 30°C for 2 hrs using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) and were co-transcriptionally capped (8:1 cap analog to GTP for ∼90% capping efficiency ...
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bioRxiv - Genomics 2023Quote: The template genomic DNA was fragmented by sonication to an insert size of 350 bp using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample and sequenced using an Illumina Hiseq 2500 PE150.
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bioRxiv - Genomics 2022Quote: ... Library preparation on samples was conducted using manufacturer’s protocols for NEBNext Ultra II Dna Library Prep Kit (New England BioLabs, CAT#E7645) and NEB Multiplex Dual Index oligos (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation module (NEB #E7490). Libraries were sent to Novogene for Illumina Hi-Seq ...
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bioRxiv - Genomics 2023Quote: Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, cat. No E7645S and E7335S) and amplified using 14 PCR cycles following the manufacturer’s instructions with slight modifications as previously described by us in detail elsewhere25 ...
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bioRxiv - Developmental Biology 2023Quote: ... This plasmid was modified to include a C-terminal V5 tag using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For arnt1-myc ...
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bioRxiv - Biochemistry 2023Quote: ... A linear expression cassette of pD912-PGAP-Hp AMPD-His10 was assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). Primers were designed using the NEBuilder Assembly Tool ...
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bioRxiv - Genetics 2023Quote: ... cDNA and sequencing libraries were generated from 500 ng of fresh RNA samples with 10 cycles of PCR with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760). After quality checking using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was normalized for cDNA conversion using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Massachusetts, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of total RNA was used as a template for cDNA synthesis with the LunaScript® RT SuperMix Kit (#E3010, New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... using a minimum of 20 bp overlapping regions between DNA fragments with custom made kit Plasmid selection and verification after the transformation were checked using the OneTaq® 2X Master Mix with Standard Buffer (NEB). Plasmids were purified using the Qiagen plasmid purification kit according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... was modified to introduce BsaI cut site and single sgRNAs were cloned into it by using Golden Gate Assembly kit (BsmBI-v2, New England Biolabs #E1602). All single sgRNAs used in this study are listed in Supplementary Table 5 ...
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bioRxiv - Biochemistry 2023Quote: ... A 2 µL aliquot of the annealed oligonucleotides was used as template for in vitro RNA transcription by HiScribe T7 quick high yield RNA Synthesis kit (New England Biolabs, MA). The transcribed RNA was purified using RNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... Library preparation was done according to NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs) including polyA enrichment and addition the of ERCC RNA spike-ins ...