Labshake search
Citations for New England Biolabs :
8201 - 8250 of 10000+ citations for Human Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... DNase-treated samples were dissolved in 10 μl water and used for library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit and Indexing primers (E7760, and E7335, respectively, both NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified ChIP DNA was used for library generation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... The sgRNA was constructed by annealing sense and anti-sense single stranded oligonucleotides containing 5’ Bsa1 restriction overhangs and was inserted into Bsa1 linearized pDR274 with the Quick Ligase Kit (NEB) (Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... The kinase-inactive mutant was generated by a two-step PCR method using the Phusion High-fidelity kit (New England Biolabs). In the first step ...
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bioRxiv - Cell Biology 2020Quote: ... The AP-3 sorting mutant was regenerated in MigR1-PI4KIIα-GFP using the Q5 site-directed mutagenesis kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... RNA with RIN>8 was used to prepare transcriptomic libraries using the NEBNext Ultra RNA library prep kit (New England Biolabs). High throughput RNA-Sequencing was performed at the NIDDK Genomic Core Facility (NIH ...
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bioRxiv - Synthetic Biology 2019Quote: ... Mutagenesis was performed using the partial overlapping primer design method or using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... We prepared D3839 libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645S/L) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... ethanol purified used to produce barcoded RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on Illumina NextSeq 500.
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bioRxiv - Genomics 2020Quote: ... and used to produce barcoded RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on Illumina NextSeq 500.
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bioRxiv - Molecular Biology 2019Quote: ... and library preparation was completed using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (New England Biolabs), both following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs E7645S) with dual indexing (New England Biolabs E7600S ...
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bioRxiv - Cancer Biology 2020Quote: ... eluted in 8 μl and in vitro transcribed (with the beads) at 37 °C overnight for linear amplification using the T7 High Yield RNA polymerase IVT kit (NEB). Following IVT ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using the NEBNext® Ultra II DNA Library Prep Kit for Illumina® (New England BioLabs, E7645) with starting input of 500ng of total RNA per sample ...
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bioRxiv - Genetics 2020Quote: ... Isolated mRNA was fragmented and thereafter reverse transcribed to cDNA using NEBNext ultra RNA library preparation kit (New England Biolabs). The cDNA products were purified using AmpureXP beads (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... ChIP-seq DNA libraries were prepared from the purified ChIP DNA and input DNA using the NEBNext ChIP-seq Library Prep Kit (E6200, NEB). Libraries were prepared from 8-15 ng of fragmented ChIP or input DNA ...
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bioRxiv - Plant Biology 2020Quote: ... The RNA-seq libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s instructions and the high-throughput sequencing was performed with the Illumina NovaSeq6000 platform ...
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bioRxiv - Synthetic Biology 2019Quote: ... The synthesized RBS-Bdh-FLAG insert was resuspended in water to a final concentration of 10 ng/µL and was assembled with linearized pBBR1-MCS2 plasmid using NEBuilder High Fidelity DNA assembly kit (New England Biolabs) using 50 ng of pBBR1-MCS2 and 100 ng of RBS-Bdh-FLAG insert ...
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bioRxiv - Biochemistry 2019Quote: ... expression constructs containing WT or mutant full-length human KAP1 preceded by a triple FLAG tag and linker sequence MDYKDHDGDYKDHDIDYKDDDDKGSGG were assembled in pLEXm (35) with the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). The following plasmids were used ...
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bioRxiv - Plant Biology 2021Quote: ... All other mutations of the constructs used for in vitro and in vivo assays were introduced using a Q5 site-directed mutagenesis kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... PCR mutagenesis to create site-directed mutants of malM 3’-UTR and cspE 3’-UTR was conducted with the Q5 site-directed mutagenesis kit (New England Biolabs) using end-to-end primers designed with NEBaseChanger ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μl cDNA reaction mix was amplified by adding 25 μl of Long Amp Taq 2x Master Mix (NEB-kit), 1.25 μl SR primer for Illumina (NEB-kit) ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing adaptors were then added on using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs #E7645S) following the manufacturer’s instructions for a 200 bp insert size distribution and using AMpure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were generated using the NEBnext small RNA library preparation kit according to instructions (E7580S, New England BioLabs).
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bioRxiv - Microbiology 2020Quote: ... All pcDNA3.1 P deletion mutants in the full-length construct were generated by using the Q5 site-directed mutagenesis kit (New England BioLabs), following the manufacturer recommendations ...
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bioRxiv - Genetics 2020Quote: ... The RNA was then diluted 1:50 and 2 mL were used to perform a one-step qPCR protocol using Luna Universal One-step qPCR kit (NEB). Two primer sets were used ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: The libraries were generated by using the NEBNext ultraII DNA library prep kit for Illumina coupled with NEBNext multiplex oligos for Illumina (New England Biolabs) with few modifications optimized for small fragments (detailed in dx.doi.org/10.17504/protocols.io.wvgfe3w. ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: RNA-seq libraries were generated by using NEBNext ultra II directional RNA library prep kit coupled with NEBNext multiplex oligos for Illumina (New England Biolabs) and following the manufacturer’s directions ...
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bioRxiv - Plant Biology 2021Quote: ... or the 2 kb region upstream of the translational start site of CaPR4 were inserted in front of the GUS reporter gene in vector pRB-bar-GUS using the NEBuilder recombinational cloning kit (NEB).
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bioRxiv - Synthetic Biology 2021Quote: ... an equal volume of RNA lysis buffer was added and RNA isolated using Monarch Total RNA Miniprep Kit (New England Biolabs). Each sample was treated by DNase I twice ...
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bioRxiv - Synthetic Biology 2021Quote: ... We ligated the vector with the inserts through Gibson assembly (Gibson et al., 2009) using NEBuilder HiFi DNA assembly kit (New England Biolabs). All primers and oligos used including sequencing primers (Integrated DNA Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... These four oligos were then assembled with PCR amplified pUA66 vector using NEBuilder HiFi DNA assembly kit (New England Biolabs). The resulting pMV001 plasmid assembly mix was then used to electroporate Top10 E ...
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bioRxiv - Physiology 2021Quote: ... The in vitro transcription of ayRhp1 capped mRNA (cRNA) was performed with HiScribe T7 ARCA mRNA kit (New England Biolabs) on SphI linearized pGEM-HE-ayRhp1 vector followed by column purification (RNeasy MiniElute Cleanup Kit ...
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bioRxiv - Evolutionary Biology 2021Quote: Library preparation was done with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... chromatin from both IP and ‘Input’ samples were eluted and de-crosslinked at 65°C for 16 hours followed by DNA isolation by PCR and DNA cleanup kit (NEB). Enrichment of AR and MED1 at specified enhancers were quantified by qPCR using specific primers ...
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bioRxiv - Microbiology 2019Quote: ... whereas KAPA HyperPlus libraries and libraries sequenced on the Ion Torrent PGM platform were quantified by qPCR using the NEBNext Library Quant Kit for Illumina (New England BioLabs) or the KAPA Library Quantification Kit for Ion Torrent platforms (Figure 1) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we sheared total pooled DNA into ∼300–400 bp fragments in an ultrasonicator and used it to build sequencing libraries with an NEBNext Ultra DNA library prep kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were purified with a 0.8x bead to library ratio and eluted in 30μL EB buffer and qPCR quantified using the NEBNext Library Quant Kit for Illumina (New England BioLabs Inc.). Purified libraries were pooled equimolarly according to the qPCR results and sequenced at the GeoGenetics Sequencing Core ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of RNA was used as starting material to make libraries following manufacturer instructions for NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). Two or more biological replicates were used for making libraries in parallel ...
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bioRxiv - Molecular Biology 2021Quote: An aliquot of the DNA extract was placed into the NEBNext Ultra II Library preparation kit (New England Biolabs®) following manufacturer’s instructions with the following modifications ...
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bioRxiv - Molecular Biology 2021Quote: Samples were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Cat # E7645) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Microbiology 2020Quote: ... and were fused to fragment TRL2(Δgfp)-NH using the NEBuilder HiFi DNA Assembly Cloning kit (NEBuilder, NEB, Cat. #M0491) to create plasmids pTRL2-NH-EUO and pTRL2-NH-HrcA ...
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bioRxiv - Microbiology 2021Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... The RNA-Seq libraries were prepared from total RNA using the NEBNext Ultra II RNA Sample preparation kit (NEB, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Transcription of pgRNA constructs were carried out using a Hiscribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), after linearization of the DNA plasmid using HindIII ...
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bioRxiv - Microbiology 2021Quote: ... The Y-adaptor was ligated to the ends of the fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were gel purified and transcribed in vitro using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). The transcribed sgRNAs were purified using the RNeasyTM mini kit (Qiagen).
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bioRxiv - Cell Biology 2020Quote: InsertTAG-pcDNA FRT/TO was created by mutagenesis of YFP-pcDNA5 FRT/TO39 to replace YFP with NheI and XhoI restriction sites using the primers ccctcgagGATATCACAAGTTTGTACAAAAAAGC (forward) and tggctagcAAACGCTAGAGTCCGGAG (reverse) and the Q5 Site-Directed Mutagenesis Kit (E0554S, New England Biolabs). BioID2-pcDNA5 FRT/TO was created by restriction digestion of BioID2 from myc-BioID2-MCS (74223 ...
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bioRxiv - Genomics 2019Quote: ... Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB). All end preparation ...