Labshake search
Citations for New England Biolabs :
8101 - 8150 of 10000+ citations for Human Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... After elution in 5 ml of elution buffer the extracted raw DNA was concentrated on a silica column from the Monarch Genomic DNA purification Kit from NEB.
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bioRxiv - Microbiology 2021Quote: ... RNA levels of target proteins were subsequently quantified by using RT-with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler using gene-specific primers ...
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bioRxiv - Cancer Biology 2021Quote: Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (NEW ENGLAND BioLabs, Inc., UK). The isolation of mRNA was performed using the Poly(A ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (NEWENGLAND BioLabs, Ipswich, MA, UK). Additionally ...
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bioRxiv - Microbiology 2021Quote: ... The V1-V2 region of the 16S rRNA genes was amplified with Q5 High-Fidelity Polymerase Kit (New England Biolabs). (F’:AATGATACGGCGACCACCGAGATCTACAC-TATGGTAATT-CC-AGMGTTYGATYMTGGCTCAG ...
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bioRxiv - Neuroscience 2020Quote: ... pSico Nectin-3-shRNA and scramble-shRNA constructs were altered before transfection to artificially replicate Cre-excision using site-directed mutagenesis (Q5 site directed mutagenesis kit: New England Biolabs). The GFP-stop sequence and one loxP site were removed from pSico using the following primers ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 µg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs). Selected genes were amplified by quantitative real time PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... and used to generate a strand-specific library using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Nascent RNA-seq was conducted as previously described (Crump et al. ...
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bioRxiv - Molecular Biology 2022Quote: Library preparation was performed at the University of Texas Genomic Sequencing Facility using the NEBNext Small RNA library preparation kit (E7330, New England Biolabs). Samples were prepared with 14 cycles of PCR and the final product was size selected using a 3% gel cassette on the Blue Pippin instrument (Sage Sciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... — Primers A995 to A998 were designed to make point mutations of ponA2 as shown in Supplementary Table using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). After mutations were confirmed by sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... The extracted RNA was then used to synthesize cDNA using ProtoScript® First Strand cDNA Synthesis Kit (New England Biolabs). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2022Quote: ... and oligos annealed with adapters to the sense 5’ - CACC and antisense 5’-AAAC were ligated using Quick Ligase Kit (New England Biolabs). Ligated product was transformed into stabl3 competent E ...
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bioRxiv - Physiology 2022Quote: ... and Poly-A mRNA-seq libraries from such samples were prepared using the Ultra II Directional RNA Library kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Amino acid substitutions and domain deletions were made using either Gibson assembly or the Q5 Site-Directed Mutagenesis Kit (NEB). Isogenic single and double mutant strains were generated via haploid mating ...
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bioRxiv - Neuroscience 2022Quote: ... Adaptors from the LSK-109 sequencing kit (Nanopore) were then ligated onto 42ul of pooled CRISPR cleaved DNA using NEBNext Quick T4 Ligase (New England BioLabs) and incubated at room temperature for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... mutations of interest were introduced into the pMT-Ed::GFP::DlgPDZ3-SH3-HOOK-GUK plasmid (Garcia et al., 2014) using the NEBaseChanger site directed mutagenesis kit as directed by the manufacturer (NEB). To generate the cytosolic pMT-GFP: ...
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bioRxiv - Genetics 2022Quote: ... PCR products were run on an agarose gel and desired bands were isolated by gel extraction (QIAquick Gel Extraction Kit, New England Biolabs) and were eluted in ddH2O ...
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bioRxiv - Developmental Biology 2022Quote: ... Sequencing libraries were prepared from 100ng of purified total mRNA using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA extractions were shipped on dry ice and whole-retina transcriptome libraries were prepared from total RNA using the NEBNext Ultra RNA library preparation kit for Illumina (New England Biolabs) at Novogene’s sequencing facilities in Beijing ...
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bioRxiv - Developmental Biology 2022Quote: ... Linearized DNA was used as a template and RNA synthesis was performed using the HiScribe SP6 RNA Synthesis Kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The purified DNA template was subjected to in vitro transcription by T7 RNA polymerase using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). After being treated with DNase I (Takara Bio) ...
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bioRxiv - Microbiology 2022Quote: Plasmids were constructed by amplifying the sequence of interest using the MG1655 genomic DNA as template and inserting it into the desired vector using either the Gibson Assembly Cloning Kit (New England Biolabs) or by digestion with the indicated restriction enzymes followed by ligation using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... The coding sequences of uvGFP and mCherry were then PCR amplified and cloned between the KpnI site using the NEBuilder kit (New England Biolabs). The sequences of LM4 ...
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bioRxiv - Microbiology 2022Quote: ... DNA libraries were prepared from extracted gDNA using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB). DNA was purified and size selected using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... The pooled oligos were next assembled into the NotI-HF-digested plasmid pSAC200 using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) and transformed via electroporation into Endura Electrocompetent E ...
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bioRxiv - Microbiology 2022Quote: The copy number of viral RNAs were titrated by qRT-PCR using Luna Universal Probe One-Step RT-qPCR Kit (NEB) and LightCycler 96 instrument (Roche) ...
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bioRxiv - Genomics 2022Quote: Real-time PCR (RT-PCR) amplification of RNA was performed following the Luna Universal One-Step RT-qPCR kit (New England Biolabs). A CFX 96 Touch Real-Time PCR Thermocycler/ Detection System (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: Site-directed mutagenesis to generate alanine or tryptophan substitutions at H2 positions in NIP constructs was done by using the mutagenesis primers listed in Table SI and the Q5 Site-Directed Mutagenesis Kit (NEB) as described previously (50) ...
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bioRxiv - Genomics 2022Quote: ... DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library Kit for Illumina (New England Biolabs) following manufacturers’ protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Dual-indexed mNGS library preparations for the samples were miniaturized and performed in 384-well format with NEBNext Ultra II RNAseq library preparation kit (New England Biolabs) reagents ...
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bioRxiv - Developmental Biology 2022Quote: miRNAs and total mRNA were extracted from a pool of 10 zebrafish hindbrains using the miRVana miRNA Isolation kit and gDNA removed using DNase1 (NEB). Reverse transcription was performed with either TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S). Transiently transfected cells were stained the following day with mAbs conjugated using DyLight® 650 Conjugation Kit (Fast ...
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bioRxiv - Microbiology 2022Quote: ... Mutations were introduced into the phCMV-Spike plasmids using the mutagenic primers and the Q5 site directed mutagenesis kit (NEB). In addition ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were generated by the Genome Analysis and Technology Core at the University of Virginia using oligo dT-purified mRNA from 500ng of total RNA and the NEB Next Ultra RNA library preparation kit (New England Biolabs), and 50-bp single-end sequencing was performed on the Illumina NextSeq500 platform (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to a sequencing ready library using the NEBNext Ultra II Directional RNA library kit (New England Biolabs). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... 30 ng of total RNA was used for next-generation library preparation under the guidelines of the NEBNext Ultra DNA Library Prep Kit for Illumina (E7370; NEB). The libraries were loaded for 2 × 150 bp pair-end sequencing using Illumina Hiseq 2500 ...
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bioRxiv - Genetics 2022Quote: ... The resulting gDNA fragments were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Bead-based size selection was used to enrich for 200 bp fragments prior to a 21-cycle splinkerette PCR utilizing a custom Tn5-enriching forward primer (oAM055 ...
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bioRxiv - Cell Biology 2022Quote: ... Ravikant Ranjan) was digested using BglII and EcoRV and TgVPS15 cDNA was ligated in it using Gibson assembly kit (NEB) to obtain pCT-TgVPS15-iCWT ...
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bioRxiv - Cell Biology 2022Quote: ... pIREShyg2-HA-hAdipoR2-cMYC(H348A) construct was generated using PCR-based mutagenesis (Q5-site-directed mutagenesis kit, New England Biolabs) with the following primers ...
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bioRxiv - Biochemistry 2022Quote: ... Purified RNAs were used to make the RNA-seq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760, NEB), followed by RNA-seq ...
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bioRxiv - Genomics 2022Quote: ... we prepared libraries for adaptive nanopore sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies, ONT) and the NEBNext Companion Module for ONT Ligation Sequencing (New England Biolabs). 3 μg gDNA was used as input for these libraries ...
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bioRxiv - Genomics 2022Quote: ... After 25 cycles of amplification the product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030), and eluted with 20 μl of ddH2O ...
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bioRxiv - Molecular Biology 2022Quote: ... 40 μL of each digest product were then ran on a 3% agarose gel and the appropriate band was gel extracted using a kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 40 μL of digest product was then ran on a 3% agarose gel and the appropriate band was gel extracted using a kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: Paired-end libraries were prepared according to the manufacturer’s recommendations using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). The libraries were indexed with NEBNext Multiplex Oligos kit for Illumina (96 Index Primers ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA libraries were prepared for each individual titration point using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7370, New England Biolabs) and barcoded using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 & 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for up to one month until used for purification of total RNA with the Monarch Total RNA Miniprep Kit (NEB). Five larvae were pooled for each RNA purification and homogenized in DNA/RNA protection buffer with a glass homogenizer prior to proteinase K digestion ...
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bioRxiv - Developmental Biology 2022Quote: ... and the reaction was purified with the Monarch RNA Cleanup Kit (500 micrograms) with elution in 50 µl nuclease-free water (NEB). The quality of the gRNA prep was confirmed by agarose gel electrophoresis and quantified using a NanoDrop One (Thermo Fisher).
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bioRxiv - Molecular Biology 2019Quote: ... quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs® Inc., MA, USA), and fragment size (330 bp ...
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bioRxiv - Cell Biology 2019Quote: ... we introduced flanking FRT sites 104bp upstream of the transcription start site and 99bp downstream of the end of the 3’UTR using a Q5 Site-Directed Mutagenesis kit (New England Biolabs) and the following primers: