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Citations for New England Biolabs :
8201 - 8250 of 10000+ citations for Human Complement Component 1 Q Subcomponent Binding Protein HABP1 C1QBP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... All point mutations in PLD3 and PLD4 were generated using Q5-site directed mutagenesis kit (New England Biolabs #E0554S).
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bioRxiv - Bioengineering 2024Quote: ... Nluc mRNA was synthesized using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs Inc., MA, USA) with CleanCap Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Genomics 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep kit with poly(A) mRNA isolation was used to construct RNA-seq libraries according to the manufacturer’s instructions (NEB). Sequencing reads were aligned to hg19 build of the human genome using HISAT2 (Kim et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#). Libraries were pooled and sequenced on an Illumina NextSeq 550 using paired-end sequencing of 42 bases per read.
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bioRxiv - Cell Biology 2024Quote: MHC-ftz-Δi mRNA was synthesized in vitro using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) with MHC-ftz-Δi in pcDNA3.0 (30 ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared by on-bead reactions using the NEB Next Ultra II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand ...
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were constructed using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) using ChIP products from “OSC ChIP” and “Ovary ChIP” ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were then constructed using the NEBNext Ultra II RNA Library prep for Illumina kit (NEB#E7760L) NEBNext Poly(A ...
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bioRxiv - Microbiology 2024Quote: ... an equimolar pool of all amplicons was prepared and purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). After Qubit quantification using Qubit® dsDNA HS Assay Kit and Qubit 2.0 fluorometer (Thermo Fisher ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... Library preparation was carried using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103). Sequencing was performed on an Illumina HiSeq4A to produce paired-end reds of 125 bp ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, #E7103) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Deglycosylation of total brain homogenates was performed according to the PNGase F kit instruction manual (#P0704S, New England Biolabs). 40µg of protein were digested with 1,000 U of PNGase F enzyme.
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bioRxiv - Immunology 2024Quote: ... mRNA was purified from 4 µg of total RNA with a magnetic mRNA isolation kit (New England Biolabs, S1550S). cDNA was synthesized from mRNA samples with M-MLV (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... The libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA), and the preparation involved a polyA selection method ...
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bioRxiv - Neuroscience 2024Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) and subsequently sequenced by Illumina NovaSeq 6000 or Illumina NextSeq 500/550 (LabGenomics ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs), validated on TapeStation (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA (300 ng) was depleted of ribosomal RNA using the NEBNext rRNA Depletion Kit (NEB Cat#E6310). rRNA-depleted RNA was purified using RNAClean XP beads (Beckman-Coulter Cat#66514) ...
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bioRxiv - Neuroscience 2024Quote: ... Mutations of PICK1 (KD-AA and 5K-E) were made with Q5® Site-Directed Mutagenesis Kit (NEB #E0554S). The constructs were purified by NucleoBond® Xtra Midi EF (MACHEREY-NAGEL) ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs, USA). 4 μL of cDNA was added to 1 μL of pre-purchased primers (QuantiTect primers (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... After DNA isolation libraries were prepared with the NEBNext ULTRA II DNA Library Prep Kit for Illumina (NEB, US) according to manufacturer’s specification with the NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: We prepared the DNA extracts into sequencing libraries following the NEBNext Ultra II FS DNA Library Prep Kit (NEB) according to manufacturers’ instructions but replaced the NEBNext Adapters with Y-Adapters ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and gel excision of DNA between 150 bp and 1,500 bp was performed using the Monarch DNA Gel Excision Kit (NEB). Concentrations of the purified libraries were measured using PicoGreen (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: Small RNA libraries from immunoprecipitated RNAs or total RNA were prepared using Small RNA Library Prep Kit (E7300, NEB) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified DNA was inserted into the Hsp68-lacZ vector using the Gibson Assembly kit (E2611S, New England Biolabs). The resulting constructs were verified ...
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bioRxiv - Developmental Biology 2024Quote: ... and both reverse transcription and PCR were performed using the Luna One-Step Universal RT-qPCR kit (NEB, E3005S) and carried out on the Mic qPCR Machine (BioMolecular Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... and mRNA was transcribed in vitro from the templates using HiScribe T7 ARCA mRNA Kit with Tailing (NEB, E2060) or mMESSAGE mMACHINE SP6 Transcription Kit (Thermo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, #E7760S) and sequenced on an Illumina NextSeq2000 platform to generate a minimum of 25-30 million 150-bp paired-end reads per sample.
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bioRxiv - Genomics 2024Quote: ... Poly-A selected libraries were prepared with a NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced using the NovaSeq 6000 (Ilumina ...
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bioRxiv - Genomics 2024Quote: Methylation enrichment of cfDNA was performed using the EpiMark Methylated DNA Enrichment Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and p.V126V were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, MA; catalog no. E0552). Primers used for site-directed mutagenesis are given in Appendix 1-table 7 ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645S) with the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... and prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manual ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation was performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs; USA) with 1 μg total RNA per sample ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were then either purified directly using Monarch PCR and DNA Cleanup Kit (New England Biolabs, Hitchin, UK), or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 25 °C) using a custom methionine(-) Flexible In vitro Translation system composed by PURExpressTM (ΔRF123) Kit (New England Biolabs) solution B ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was prepared after polyA mRNA selection using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). Sequencing was performed on Illumina NovSeq600 using a 2×150 paired end configuration ...
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bioRxiv - Microbiology 2024Quote: ... double-stranded cDNA libraries were generated by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Libraries for sequencing were prepared using extracted RNA and the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (New England Biolabs). Quality of all RNA and library preparations were evaluated with BioAnalyzer 2100 (Agilent).
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... and HCN4 Δ1-185 deletion clones were made using a site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) and a codon-optimized HCN4 plasmid in the pTwist-CMV-WPRE-Neo vector synthesized by Twist Biosciences.
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs). RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs). For both types of libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries for RNA-seq were made with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation and multiplexing for ChIP- seq libraries were performed using the NEBNext ChIP-seq library Prep kit (NEB). The sequencing of ChIP-seq and input control libraries was performed using Illumina HiSeq-2500.
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bioRxiv - Immunology 2024Quote: Bulk RNA sequencing libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on a high throughput NextSeq 500 device ...
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bioRxiv - Synthetic Biology 2023Quote: ... In vitro transcription of sgRNAs was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Then ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB, E6420S) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra™ DNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: Libraries in this paper were prepared using NEB’s rRNA Depletion Kit (Bacteria) for rRNA removal with beads (NEB #E7860). Post rRNA removal samples were prepared for either Illumina or Singular Sequencing using NEBNext Ultra II RNA Library Prep Kit for Illumina with Beads (NEB#E7775) ...