Labshake search
Citations for New England Biolabs :
8151 - 8200 of 10000+ citations for Human Complement Component 1 Q Subcomponent Binding Protein HABP1 C1QBP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... ChIP samples were processed for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instruction and sequenced on a Next-Seq 500 Sequencer (Illumina ...
-
bioRxiv - Genomics 2022Quote: ... Library preparation was performed using NEB Next® Ultra™ RNA Library Prep Kit (NEB, San Diego, CA, USA), employing AMPure XP Beads to purify the products of the reactions during the library prep ...
-
bioRxiv - Genetics 2022Quote: Libraries were generated with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). mRNA was enriched from total RNA (250 ng ...
-
bioRxiv - Genetics 2022Quote: All of the plasmids created by Gibson assembly used the NEBuilder HiFi DNA Assembly kit (New England Biolabs, #E2621). To construct the URA3 RRM3 plasmid ...
-
bioRxiv - Genomics 2022Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The purified UVRh1 amplicons were cloned into the minT vector using the PCR cloning kit and following the manufacturer’s protocol (NEB). The cloned amplicons were sequenced by Retrogen Inc ...
-
bioRxiv - Plant Biology 2022Quote: ... RNA samples were used to generate sequence libraries using a library prep kit from New England Biolabs (NEB #E7530). Paired-end sequence reads of 75 bp were generated at the TUFTs genomic center at the Tufts University School of Medicine using the Hi-Seq Illumina platform ...
-
bioRxiv - Microbiology 2022Quote: ... the start codon of each uORF was mutated using a Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The sequence additions and mutations of all plasmids were confirmed by DNA sequencing (Source Bioscience).
-
bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were constructed using NEB Next® UltraTM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Plant Biology 2022Quote: ... RNA Libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced as single end reads using the NextSeq500 platform (Illumina ...
-
bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to the NEBNext Ultra RNA II directional Library Prep kit (New England Biolabs, MA, USA) after NEBNext rRNA depletion (New England Biolabs ...
-
bioRxiv - Molecular Biology 2022Quote: ... Library preparation was performed with the NEBNext Ultra DNA Library prep kit according to the manufacturer’s instructions (NEB, E7370L). 4C-seq libraries were sequenced on an Illumina Hiseq2500 platform (50 cycles ...
-
bioRxiv - Immunology 2022Quote: ... Library quality and quantity were assessed by qPCR method using NEB Next Library Quant Kit for Illumina (NEB, # E7630) and Qubit ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA of the gram-negative bacteria was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs). After isolation of the genomic DNA ...
-
bioRxiv - Microbiology 2022Quote: ... for removal of genomic DNA before it was purified using the Monarch RNA cleanup kit (New England Biolabs, USA). The RNA and potential DNA contamination concentrations were quantified using Qubit RNA BR and Qubit dsDNA HS assay kits ...
-
bioRxiv - Microbiology 2022Quote: ... ShdA III and ShdA IV homologues were synthesised in vitro using the PURExpress cell-free transcription/translation kit (NEB), from PCR products ...
-
bioRxiv - Microbiology 2022Quote: ... and libraries were quantitated with qPCR (Bio-rad CFX96 Touch Real-Time PCR, NEB Library Quant Kit for Illumina). Libraries were normalized to 0.5 nM and pooled ...
-
bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Antisense strand DNA was digested in the samples using the Uracil-Specific Excision Reagent Enzyme Kit (New England Biolabs), followed by a PCR to amplify the cDNA ...
-
bioRxiv - Plant Biology 2022Quote: ... The small RNA libraries were prepared using the NEBNext Small RNA Sample Prep kit (New England Biolabs, Ipswich, MA). High throughput sequencing was performed with NovaSeq-6000 (Illumina ...
-
bioRxiv - Plant Biology 2022Quote: ... followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420). RNA libraries were sequenced on an Illumina NovaSeq 6000 platform (PE150 bp) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting plasmid backbone of the linearized vector was run on the 0.8% agarose gel and purified by Monarch DNA Gel Extraction Kit (NEB). The oligos for the inserted sgRNA targeting sequences were synthesized with two overhangs compatible with the linearized vector and complementary to each other ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® was used (New England Biolabs, MA, USA). For sequencing ...
-
bioRxiv - Plant Biology 2022Quote: ... DNA fragments were inserted into plasmid vectors using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Massachusetts, USA). The primers used in this study are listed in the Supplemental Table S9 ...
-
bioRxiv - Molecular Biology 2023Quote: The following M35 derivative constructs were derived from pcDNA3.1 TOPO M35-V5/His using the Q5 site-directed mutagenesis kit (#E0554, New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... The inserts and backbone were assembled using the NEB HiFi DNA assembly kit (New England Biolabs, Ipswich, Massachusetts, USA), resulting in the final plasmids ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs). Library preparation was initiated with 10ng of total RNA ...
-
bioRxiv - Microbiology 2024Quote: ... primers oAA033-034 were used to remove mprF using the Q5 site-directed mutagenesis kit (NEB Cat. No. E0554S) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using dual-end indexing using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645L) using the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs) according to manufacturer’s instructions in combination with a poly(A ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mutations and insertions were introduced by site directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs). Oligonucleotides for site-directed mutagenesis were designed using the NEBaseChanger version 1.3.3 (New England Biolabs ...
-
bioRxiv - Physiology 2022Quote: ... cDNA was prepared following the NEBNext® Ultra™ II Directional RNA Library Prep Kit protocol (New England Biolabs). The libraries were sequenced using paired-end 150 bp sequencing on a HiSeq 4000 System (Illumina) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with a primer/probeset binding in the orf1ab region (forward ...
-
bioRxiv - Molecular Biology 2022Quote: ... and in vitro transcription was performed using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA, USA) to generate IVT mRNA according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... ChIP-Seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on a NovaSeq 6000 ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA-seq libraries were prepared with NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolabs #E7420S) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2022Quote: ... in high output runs using a NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) including rRNA depletion ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant plasmids encoding a catalytically inactive 3Dpol (3Dneg) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). Nucleotides at position 6891 and 6892 of the CVB3/28 genome were mutated from AT to GC ...
-
bioRxiv - Microbiology 2022Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using the manufacturer’s instructions (New England Biolabs). Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by T7 in vitro transcription using the HiScribe T7 In Vitro Transcription Kit (New England Biolabs, Ipswich, MA) and purification with Trizol (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... and ChIP libraries were amplified and barcoded using the NEBNext Ultra II DNA library preparation kit (New England Biolabs) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2022Quote: ... FLAG-tagged ORF3c mutant Q5Y was prepared by site-directed mutagenesis using a commercial kit (New England Biolabs, E0554S).
-
bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared as in [34] with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).
-
bioRxiv - Molecular Biology 2024Quote: ... followed by the GFP sequence were generated by using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, E2060). 1 µg mRNA was first denatured at 65°C for 3 minutes ...
-
bioRxiv - Plant Biology 2023Quote: ... In vitro transcription was performed using the HiScribe T7 ARCA mRNA (with tailing) kit (New England Biolab, http://www.NEB.com) and Xenopus oocytes were injected with 30 ng of RiSKC3 cRNA using a pneumatic injector ...
-
bioRxiv - Cell Biology 2024Quote: ... the pellet was air dried and then resuspended in DNA Elution Buffer (Monarch DNA Gel Extraction Kit, NEB, USA). Since the RPS27AP5 sequence ...
-
bioRxiv - Bioengineering 2024Quote: ... and A-tailed using the NEB Next Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Linkers were then ligated to the DNA using the NEB Next Ultra II Ligation Master Mix and Ligation Enhancer (New England Biolabs) ...
-
bioRxiv - Biochemistry 2024Quote: ... C-terminal Cx36 mutants were generated using the Q5 site directed mutagenesis kit (E0554S; New England Biolabs, Ipswitch, MA) and corresponding primer pairs introducing gaps into the Cx36 coding sequence ...