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Citations for New England Biolabs :
8151 - 8200 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Reporter gene fusions for cis-regulatory analysis of terminal identity genes were made using either PCR fusion [68] or Gibson Assembly Cloning Kit (NEB #5510S). Targeted DNA fragments were fused (ligated ...
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bioRxiv - Developmental Biology 2019Quote: ... of unc-3 and lin-39 were amplified and then ligated to cholinergic (cho-1, unc-3) or GABAergic (unc-47) promoters using Gibson Assembly Cloning Kit (NEB #5510S). For unc-3 RNAi ...
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bioRxiv - Genetics 2020Quote: ... 50 ng of each amplicon was end-repaired and adenylated using an NEBNext Ultra End Repair/dA-Tailing Module kit (NEB, USA) and purified using AMPpure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2019Quote: ... were made from cells isolated by FACS from each patient sample using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB E7370) and single indexed using NEB E7335 or NEB E7500 ...
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bioRxiv - Molecular Biology 2019Quote: ... Library preparation was conducted using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England BioLabs, USA) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA-seq libraries were constructed using NEBNext® Ultra RNA Library Prep Kit for llumina® (New England Biolabs, MA, USA) and sequenced with the Illumina HiSeq X-Ten platform (Illumina ...
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bioRxiv - Synthetic Biology 2020Quote: ... MMR-DN mutant genes were subsequently generated via site-directed mutagenesis using either Q5 Site-Directed Mutagenesis Kit (NEB, catalog E0554S) or QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Biochemistry 2020Quote: cDNA was reverse-transcribed from donor 1 and donor 2 RNA samples using the ProtoScript II first strand cDNA synthesis kit (NEB #E6560) with oligo dT priming ...
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bioRxiv - Genomics 2020Quote: ... 48hr APF TRAP libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, product E7760S) and 10-12 day adult TRAP libraries were prepared with the NEBNext Ultra RNA library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2019Quote: The cDNA encoding full-length spliced DIGIT was in vitro transcribed using the HighScribe T7 High Yield RNA synthesis kit (NEB, E2040s). Cyanine 3-UTP (Enzo Life Sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... reverse primer ACA AGG GCT TTC TCT CAT AAT GAG ATG CTC and the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Cells were transfected with 50 nM siRNA using Dharmafect 1 for 48 h ...
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bioRxiv - Genetics 2020Quote: ... The purified DNA (200 ng) was subject to library preparation using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB). Libraries with barcodes were pooled and sequenced on the Illumina HiSeq 1500 platform ...
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bioRxiv - Genetics 2020Quote: Libraries were prepared from 2 ng of DNA using the NEBNext Ultra II FS DNA library prep kit (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and mutated into a constitutively active form (pEGFP-C1-ERαY537S)29 using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E054S).
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bioRxiv - Genomics 2021Quote: ... and then ligated into pcDNA 3.1 3xHA cut with HindIII and NotI using the Quick Ligation kit (New England Biolabs cat # M2200L) following manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... protocol using the PCR Barcoding Expansion 1-12 (EXP-PBC001) kit (ONT) and LongAmp Taq 2× Master Mix (New England Biolabs, MA) with the following thermocycling conditions ...
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bioRxiv - Microbiology 2021Quote: ... These promoters and first ten amino acids of YmoA and H-NS were fused in frame to lacZ and cloned into a BamHI- and SalI-digested pFU99a using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Inc) electroporated into Y ...
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bioRxiv - Molecular Biology 2020Quote: ... The domain deletion mutants of pCS2+ FLAG-tagged SRSF1 construct were generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The primers sequences are given in Supplementary Table S1.
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bioRxiv - Cell Biology 2020Quote: ... Libraries were created using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and sequencing performed on an Illumina NextSeq 500 using a NextSeq High Output Kit ...
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bioRxiv - Genomics 2020Quote: ... 700 nanograms of extracted DNA was utilized as starting material in NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs, USA). The DNA was fragmented using focused ultrasonicator (Covaris M220 ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared with random primers using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760L) with NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Pathology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA) following manufacturer’s recommendations and index codes were added ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries from the extracted RNA were generated using the NEBNext® RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2021Quote: ... Standard PCR-based amplification to add overlap-adaptors and Gibson-style assembly methods (HiFi Assembly Kit, New England Biolabs, Ipswich, Massachusetts) were used to assemble the final vectors ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplification of the relevant regions was performed using Thermoscientific™ Phire™ Hot Start II DNA Polymerase on plasmids isolated from stock cultures with the QIAprep® Spin Miniprep Kit followed by linearization with appropriate restriction enzymes (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... PCR products (donor-5’biotin, donor-unmodified) were gel purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020S) and eluted in 20μl embryo transfer water (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 18 hours and the plasmid was extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Mutations to the specificity of guides in pTREX-n-Cas9-noTags was performed using a Q5 mutagenesis kit (NEB, Ipswich, Massachusetts). Guides targeting the mitochondrial glutamate dehydrogenase (mGDH ...
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bioRxiv - Microbiology 2021Quote: ... then an RNAseq library was prepared with the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was then performed in single read mode on a HiSeq 3000 system ...
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bioRxiv - Microbiology 2021Quote: ... Pair-end reads libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) and sequenced on an Illumina Hiseq platform ...
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bioRxiv - Microbiology 2021Quote: ... and 500ng DNA was used as input for the NEBNext Ultra II FS DNA Library Prep kit (New England Biolabs, USA), following the manufacturer’s instructions with modifications as follows ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA from each library was purified from an entire 1ml cell pellet using the Monarch Genomic DNA Purification Kit (New England Biolabs, USA). Samples were eluted in 50µl nuclease-free water ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sequencing libraries were generated from the qualified DNA samples using the NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s protocol ...
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bioRxiv - Zoology 2020Quote: The 16S rRNA and gltA amplicons from PCR assays were cleaned with the ExoSAP-IT kit (E1050, New England Biolabs, US) and Sanger sequenced ...
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bioRxiv - Systems Biology 2019Quote: ... The final pool was run on an Agilent Bioanalyzer dsDNA High Sensitivity chip and quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L). The quantified pool was hybridized at a final concentration of 400 pM and sequenced paired-end on the Illumina NovaSeq6000 platform using a S2 flowcell at 2×151 bp read lengths.
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bioRxiv - Systems Biology 2019Quote: ... 1000 ng per sample was then processed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760L) and included polyA-enrichment using NEBNext Poly(A ...
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bioRxiv - Microbiology 2020Quote: ... The digested samples were loaded on an agarose gel to detect and purify fragments ranging from 100-500 bps using the Monarch® DNA Gel Extraction Kit (New England Biolabs). The fragmented gDNA was incubated for 1 hr with 10 μM PhuS in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting amplicon was assembled with a hHBB-Nluc sequence that lacked a 3’ UTR but maintained a unique barcode using a NEBuilder HiFi Assembly Kit (NEB, ES2621).
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared following the protocol from NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7770S) and indexes (NEB #E7335S and #E7500S).
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Immunology 2020Quote: ... The mAbs were reverted to the UCA sequence in a step-by-step manner using the Q5® Site-Directed Mutagenesis Kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were cloned and performed Gibson assembly together with the triple-digested vector pCVD443 (NEBuilder HiFi DNA Assembly Cloning Kit E5520S, New England BioLabs Inc.). Assembly mixture was then used to transformed E ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA obtained from the ChIP-assays was adaptor-ligated and amplified using the NEBNext multiplex oligo kit (New England BioLabs, E7335S) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Library preparation was done using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs, Frankfurt, Germany) and the protocol for use with inputs of ≥ 100 ng DNA ...
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bioRxiv - Biochemistry 2020Quote: Mutagenesis of the 5-HT2C construct was performed according to the Q5® site-Directed Mutagenesis Kit protocol (New England BioLabs). In brief ...
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bioRxiv - Genomics 2019Quote: RNA from P2 and P9 hypothalamus and cortex was prepared for RNA-seq library using the NEBNext rRNA Depletion kit (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S) ...
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bioRxiv - Plant Biology 2019Quote: ... Library preparation was carried out according to manufacturers instructions (NEB Next Ultra DNA Library Prep Kit for Illumina, New England BioLabs Inc) and paired end sequencing was performed on the Illumina platform at the Cold Spring Harbor laboratory (New York ...
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bioRxiv - Microbiology 2019Quote: ... plus 280 nM each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Immunology 2019Quote: ... and libraries were prepared from 2 ng of total RNA using the NEBNext low input kit (New England Biolabs, Hitchen, U.K.). Libraries were assessed for correct size distribution on the Agilent 2200 TapeStation and quantified by Qubit DNA High Sensitivity assay (Thermo Fisher Scientific ...