Labshake search
Citations for New England Biolabs :
751 - 800 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... Plasmids were linearized with 0.25 μL of NotI-HF (NEB, Ipswitch, MA, USA) for 30 min at 37°C in a 20 μL reaction at 1 × 1010 copies/μL ...
-
bioRxiv - Microbiology 2021Quote: ... Sequence-verified plasmids were transformed into chemically competent NiCo21(DE3) cells (NEB, #C2529H) following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The resulting plasmid lentiMPRA library was electroporated into 10-beta competent cells (NEB) using a Gemini X2 electroporation system (BTX ...
-
bioRxiv - Immunology 2020Quote: ... the plasmid was cut with EcoRI and BamHI restriction enzymes (NEB, Ipswich, MA), and BiTE cDNA excised from the pAmp vector was directly ligated with T4DNA ligase ...
-
bioRxiv - Microbiology 2020Quote: ... The positive plasmids were linearized using SpeI restriction enzyme (New England Biolabs, UK) and the concentrations were measured using dsDNA high sensitivity assay kit in Qubit 3.0 fluorometer (Thermofisher) ...
-
bioRxiv - Molecular Biology 2020Quote: ... the plasmid was ligated by T4 ligase (New England Biolabs, cat# 101228-176) and transformed into Agilent XL1-Blue Electroporation-Competent Cells (Agilent ...
-
bioRxiv - Genetics 2020Quote: ... This intermediate plasmid product (pLCv2-GFP) was digested with AfeI (NEB, Cat# R0652S) and EcoRI-HF (NEB ...
-
bioRxiv - Microbiology 2021Quote: ... plasmid DNA and PCR amplicons were digested with commercial restriction enzymes (NEB, USA); DNAs were ligated using T4 DNA ligase (NEB ...
-
bioRxiv - Neuroscience 2021Quote: ... The plasmids were then digested with AflIII and XbaI (both NEW ENGLAND BIOLABS) to cut out the hU6 promoter and sgRNA sequence ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmid and insert products were digested using appropriate restriction enzymes (New England Biolabs) and ligated using T4 Ligase (New England Biolabs) ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR fragment and plasmid were digested with AscI and NheI (New England Biolabs). Because 4mt-GCaMP6f contains an NheI digestion site ...
-
bioRxiv - Cancer Biology 2022Quote: ... The resulting Attl1-mCMV-Kozak-ATRX-Attl2 plasmid was digested using KasI (NEB) and the PB-TAC-ERN plasmid (a gift from Knut Woltjen ...
-
bioRxiv - Developmental Biology 2022Quote: ... digested pCS2 plasmid (Lawson #444) with NEBuilder HiFi DNA Assembly Master Mix (NEB). The assembled plasmids were used for the transformation of chemically competent E ...
-
bioRxiv - Plant Biology 2022Quote: ... we linearized plasmid pL4440-CP in independent reactions with BglII and HindIII (NEB). Once linearized ...
-
bioRxiv - Plant Biology 2022Quote: ... After blunting the plasmid ends with Klenow enzyme (New England Biolabs, Hitchin, UK), the plasmid was religated with the T4 DNA Ligase (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... All plasmids generated were assembled using Gibson Assembly Master Mix (New England Biolabs) per manufacturer protocols ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmid vectors were then digested with KpnI-HiFi and XbaI (New England BioLabs) at 37°C for 2 hours ...
-
bioRxiv - Microbiology 2022Quote: A circularizable LY2 plasmid was digested with Esp3I (New England Biolabs catalog # R0734) and PvuI-HF (New England Biolabs catalog # R3150) ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids (Table S3) were propagated in Escherichia coli DH5α cells (NEB, United Kingdom) by growing cells in LB media without NaCl (Formedium ...
-
bioRxiv - Neuroscience 2022Quote: ... while the Ift52 plasmid was linearized with KpnI or SacI (New England Biolabs), for the synthesis of sense and antisense single-stranded RNAs (ssRNA ...
-
bioRxiv - Plant Biology 2022Quote: ... Of each entry plasmid 100 ng was combined with 1× Cutsmart buffer (NEB), 1 mM ATP ...
-
bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were treated with PreCR Repair Mix (M0309, New England Biolabs) and purified with the Monarch PCR & DNA Cleanup Kit (T1030 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2.5 uL of purified plasmids were digested with Nde I (New England BioLabs) and analyzed by 0.7% agarose gel electrophoresis.
-
bioRxiv - Microbiology 2022Quote: ... the plasmid pT7/3a coding for 4.5S RNA was linearized using BamHI (NEB) and in vitro transcription was performed as above ...
-
bioRxiv - Microbiology 2022Quote: All plasmids were generated with the NEBuilder DNA assembly kit (New England Biolabs), using a pCAGGS plasmid digested with EcoRV-HF and HindIII-HF (New England Biolabs ...
-
bioRxiv - Molecular Biology 2023Quote: pAC8-FLAG-SHLD3 truncation plasmids were generated using Gibson cloning (New England Biolabs) and a pAC8-FLAG-SHLD3 template (Setiaputra et al. ...
-
Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... After cleaning the plasmid digestion using the Monarch DNA Cleanup Kit (NEB, #T1030), the DNA concentration of the linearized plasmid was measured to determine the number of DNA molecules in the stock solution ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Discrete plasmids were constructed using the HiFi DNA Assembly kit (NEB, cat #E2621) to insert synthetic genes (IDT gBlocks or eBlocks ...
-
bioRxiv - Synthetic Biology 2023Quote: The successfully cloned plasmids were digested with PmeI restriction enzyme (New England Biolabs) to linearize prior cell transformations into either W303 or YPH499 (MATa ...
-
bioRxiv - Biochemistry 2023Quote: ... The plasmids were then transformed into DH5-alpha high-efficiency competent cells (NEB). Following transformation ...
-
bioRxiv - Developmental Biology 2023Quote: ... Plasmids and HaloTag PCR products were digested using AvrII (New England Biolabs, R0174S) and MluI (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... 75 μg of plasmid pΔmksB(gent) were digested with ApaLI (New England Biolabs) in a 500 μL reaction volume for 4 hours ...
-
bioRxiv - Microbiology 2023Quote: ... a pET-22b-based plasmid was generated using Gibson assembly (New England Biolabs). In this construct ...
-
bioRxiv - Bioengineering 2023Quote: ... 42 The resulting gBlocks were ligated into pSTEPL plasmids using Gibson assembly (NEB) and transformed into chemically competent SHuffle T7 Express E.coli (NEB) ...
-
bioRxiv - Cell Biology 2023Quote: All plasmids were constructed using the NEBuilder system (New England BioLabs, MA, USA) and enzymatic digestion and ligation (TaKaRa ...
-
bioRxiv - Microbiology 2023Quote: ... Linearized pExTra plasmid was prepared via PCR (NEB Q5 HotStart 2X Master Mix) of pExTra01 (Heller et al ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were constructed by standard restriction ligation methods using T4 DNA ligase (NEB) or using the NEBBuilder assembly kit (NEB) ...
-
bioRxiv - Biochemistry 2022Quote: The plasmid CM13d3 (Antibody Design Labs) was digested with SacI (New England Biolabs) for 10 hours at 37 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... The constructed plasmids were chemically transformed to DH5a competent cells (New England Biolabs) and then their sequences were confirmed through Sanger Sequencing by Sequetech Corporation (Mountain View ...
-
bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
-
bioRxiv - Genetics 2022Quote: ... The purified fragment and plasmid were ligated using T4 ligase (New England Biolabs). The ligation products were transferred into Ecloni 10GF’ electrocompetent cells (Lucigen ...
-
bioRxiv - Cell Biology 2023Quote: ... and pSNAPf-H2B control plasmid (Addgene #101124, New England Biolabs and Ana Egana) using KpnI and NotI ...
-
bioRxiv - Microbiology 2023Quote: ... and the pBP-lacZ plasmid was dephosphorylated using QuickCIP (all enzymes from NEB). The ligation was carried out with T4 DNA ligase (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2023Quote: ... and the linearized plasmid was re-ligated with a Quick Ligase Kit (NEB) following manufacturer instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... NEB Stable (lentiviral vectors) and NEB 5-alpha (other plasmids) (New England Biolabs) were used as cloning strains ...
-
bioRxiv - Cell Biology 2023Quote: ... and plasmid recombination was performed using NEBuilder HiFi DNA assembly (New England Biolabs). The expression plasmid encoding CFP-CAAX was constructed by inserting the CAAX sequence (KDGKKKKKKSKTKCVIM) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The repair template plasmid (pKK4) was assembled using the NEBuild HiFi Kit (NEB) from a combination of a restriction digest fragment and a synthetic gBlock Gene Fragment (IDT) ...
-
bioRxiv - Systems Biology 2024Quote: ... 2-10 µg plasmid DNA was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 4 h at 37°C and then spin-purified.
-
bioRxiv - Systems Biology 2024Quote: ... Plasmids were digested with restriction enzymes from New England Biolabs (NEB, Ipswich, MA). Ligations used T4 DNA ligase (NEB M0202 ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids pKN9 - pKN12 expressing different traB chimeras were constructed by Gibson assembly (NEB). Partial traB DNA fragments were amplified by PCR using pED208 or F as a template and primers listed in Table S2 ...