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Citations for New England Biolabs :
751 - 800 of 2293 citations for Mouse NRAP shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... 2.5 uL of purified plasmids were digested with Nde I (New England BioLabs) and analyzed by 0.7% agarose gel electrophoresis.
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bioRxiv - Microbiology 2022Quote: ... the plasmid pT7/3a coding for 4.5S RNA was linearized using BamHI (NEB) and in vitro transcription was performed as above ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... After cleaning the plasmid digestion using the Monarch DNA Cleanup Kit (NEB, #T1030), the DNA concentration of the linearized plasmid was measured to determine the number of DNA molecules in the stock solution ...
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bioRxiv - Synthetic Biology 2023Quote: ... Discrete plasmids were constructed using the HiFi DNA Assembly kit (NEB, cat #E2621) to insert synthetic genes (IDT gBlocks or eBlocks ...
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bioRxiv - Synthetic Biology 2023Quote: The successfully cloned plasmids were digested with PmeI restriction enzyme (New England Biolabs) to linearize prior cell transformations into either W303 or YPH499 (MATa ...
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bioRxiv - Microbiology 2022Quote: All plasmids were generated with the NEBuilder DNA assembly kit (New England Biolabs), using a pCAGGS plasmid digested with EcoRV-HF and HindIII-HF (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: pAC8-FLAG-SHLD3 truncation plasmids were generated using Gibson cloning (New England Biolabs) and a pAC8-FLAG-SHLD3 template (Setiaputra et al. ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were constructed using the NEBuilder system (New England BioLabs, MA, USA) and enzymatic digestion and ligation (TaKaRa ...
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bioRxiv - Microbiology 2023Quote: ... Linearized pExTra plasmid was prepared via PCR (NEB Q5 HotStart 2X Master Mix) of pExTra01 (Heller et al ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μg of plasmid pΔmksB(gent) were digested with ApaLI (New England Biolabs) in a 500 μL reaction volume for 4 hours ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were constructed by standard restriction ligation methods using T4 DNA ligase (NEB) or using the NEBBuilder assembly kit (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... a pET-22b-based plasmid was generated using Gibson assembly (New England Biolabs). In this construct ...
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bioRxiv - Biochemistry 2022Quote: The plasmid CM13d3 (Antibody Design Labs) was digested with SacI (New England Biolabs) for 10 hours at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... The constructed plasmids were chemically transformed to DH5a competent cells (New England Biolabs) and then their sequences were confirmed through Sanger Sequencing by Sequetech Corporation (Mountain View ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Genetics 2022Quote: ... The purified fragment and plasmid were ligated using T4 ligase (New England Biolabs). The ligation products were transferred into Ecloni 10GF’ electrocompetent cells (Lucigen ...
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bioRxiv - Cell Biology 2023Quote: ... and pSNAPf-H2B control plasmid (Addgene #101124, New England Biolabs and Ana Egana) using KpnI and NotI ...
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bioRxiv - Microbiology 2023Quote: ... and the pBP-lacZ plasmid was dephosphorylated using QuickCIP (all enzymes from NEB). The ligation was carried out with T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... and the linearized plasmid was re-ligated with a Quick Ligase Kit (NEB) following manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... NEB Stable (lentiviral vectors) and NEB 5-alpha (other plasmids) (New England Biolabs) were used as cloning strains ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids were then transformed into DH5-alpha high-efficiency competent cells (NEB). Following transformation ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmids and HaloTag PCR products were digested using AvrII (New England Biolabs, R0174S) and MluI (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... 42 The resulting gBlocks were ligated into pSTEPL plasmids using Gibson assembly (NEB) and transformed into chemically competent SHuffle T7 Express E.coli (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... the plasmid above was modified using the Q5 Site-Directed Mutagenesis Kit (NEB) along with the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... and plasmid recombination was performed using NEBuilder HiFi DNA assembly (New England Biolabs). The expression plasmid encoding CFP-CAAX was constructed by inserting the CAAX sequence (KDGKKKKKKSKTKCVIM) ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmids were ligated based on Gibson assembly (New England BioLabs master mix) of plasmid backbone and insert sequences ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cloning and plasmid propagation were performed using NEB Turbo (New England Biolabs; C2984H) competent E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The repair template plasmid (pKK4) was assembled using the NEBuild HiFi Kit (NEB) from a combination of a restriction digest fragment and a synthetic gBlock Gene Fragment (IDT) ...
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bioRxiv - Molecular Biology 2023Quote: ... gRNA oligonucleotides were ligated into pX458 plasmid with T4 DNA ligase (NEB, #M0202S). The ligation reaction was transformed into DH5 α-competent E ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids pKN9 - pKN12 expressing different traB chimeras were constructed by Gibson assembly (NEB). Partial traB DNA fragments were amplified by PCR using pED208 or F as a template and primers listed in Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: Both HURP plasmids were transformed into Rosetta2 (DE3) Escherichia coli cells (NEB: C29871) and grown in 2 L of LB media containing 50 μg/mL ampicillin and 25 μg/mL chloramphenicol at 37 °C in a shaking incubator (200 rpm) ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cloned plasmids were subjected to colony PCR using Taq polymerase (New England Biolabs), restriction digestion analysis and Sanger sequencing in order to confirm the clones (Table S6 ...
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bioRxiv - Microbiology 2023Quote: ... The plasmids were linearised by digestion with PacI (New England Biolabs, Ipswich, UK) and ethanol precipitated before transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were linearized using NcoI (R0197, R3193, New England BioLabs, Waltham, MA, USA) at 37°C for 1 hr ...
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bioRxiv - Cell Biology 2023Quote: ... MBP::CDC-42 coding sequence was inserted into the pMAL-c5X plasmid (NEB). The S71A mutation was introduced into the pMALc-MBP::CDC-42 plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli were performed with the Monarch® Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Biophysics 2023Quote: ... following the manufacturer’s protocol after plasmid linearization with NotI-HF (New England BioLabs). RNA cleanup was performed with the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Biophysics 2023Quote: ... we digested any remaining methylated wildtype plasmid using Dpn1 (New England Biolabs, R0176L) for 1 hour at 37°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The plasmid was linearized by digestion with SapI restriction enzyme (New England Biolabs) for 1 hour in 37 °C ...
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bioRxiv - Genetics 2023Quote: Plasmids for yeast two-hybrid experiments were constructed using NEBuilder DNA assembly (NEB), Ligation High kit (TOYOBO) ...
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bioRxiv - Molecular Biology 2023Quote: ... Line- arized plasmid standards were obtained by digesting pAAV_GFP with HindIII (NEB, R3104S) restriction enzyme.
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bioRxiv - Genetics 2024Quote: ... and digesting and ligating the insert into the pX458 plasmid using BbsI (NEB) and T4 Ligase (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... PCR product and pSIP409 plasmid were digested with NcoI and XhoI enzymes (NEB), gel purified with Monarch DNA Gel Extraction Kit (NEB) ...
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bioRxiv - Immunology 2024Quote: ... plasmid and inserted with NEBuilder HiFi Assembly cloning kit (#E5520S, New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... the plasmids were linearised by the DraI restriction enzyme (New England Biolabs; R0129), a dual cutter for the plasmid ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we PCR-amplified the plasmids’ inserts using Q5 polymerase (NEB, USA product #M0491) (see “Molecular Cloning” for protocol) ...
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bioRxiv - Plant Biology 2024Quote: ... The pJET_ZEP3-NosT plasmid was digested with AvrII and SbfI restriction enzymes (NEB). These were then ligated with T4 DNA ligase (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... Plasmids were digested with restriction enzymes from New England Biolabs (NEB, Ipswich, MA). Ligations used T4 DNA ligase (NEB M0202 ...
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bioRxiv - Systems Biology 2024Quote: ... 2-10 µg plasmid DNA was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 4 h at 37°C and then spin-purified.