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Citations for New England Biolabs :
701 - 750 of 2293 citations for Mouse NRAP shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Plasmid DNA was purified from liquid cultures using DNA miniprep kit (NEB #T1010).
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bioRxiv - Synthetic Biology 2022Quote: ... These plasmids were transformed into chemically competent BL21(DE3) cells (New England Biolabs) and single transformants selected for using ampicillin resistance.
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bioRxiv - Cell Biology 2022Quote: ... Cloning into the pGFP33 plasmid were performed using the Gibson assembly kit (NEB). ARF1 point mutations were generated by Quick-change Mutagenesis kit (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmid was maintained in T7 Express lysY/Ig Competent E.coli cells (NEB). To prepare the transposase ...
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bioRxiv - Molecular Biology 2022Quote: ... the pJFH-1T plasmid was amplified using Q5 high fidelity DNA polymerase (NEB) and the EcoRI- T7-JFH1-U4C-FW (5’- GGC CAG TGA ATT CTA ATA CGA CTC ACT ATA GAC CCG CCC C-3’ ...
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bioRxiv - Microbiology 2022Quote: ... pPVM-2A-144-poliovirus-GFP plasmid DNA was linearized with EcoRI (NEB R3101). Purified linear DNA was then used to in vitro transcribe poliovirus plus stranded RNA using MEGAscript™ T7 Transcription Kit (Thermo Fisher Scientific AM1334 ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid PHO5 AS gDNA-URA3 was then obtained by Gibson assembly reaction (NEB) using OFS_2886 and OFS_2887 to amplify pFS3892 backbone and OFS_2888 and OFS_3095 for gDNA cloning.
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bioRxiv - Biochemistry 2022Quote: ... and the plasmids coding for the remaining segments were linearized using BsmBI (NEB). Following linearization ...
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bioRxiv - Biochemistry 2022Quote: ... or 79.85 fmol supercoiled plasmid DNA (KAC1151 or MNW95) in ThermoPol buffer (NEB) with a final concentration of 10mM MgSO4 ...
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bioRxiv - Immunology 2020Quote: ... and assembled into the plasmid vector with NEB HiFi assembly (New England BioLabs). An expression plasmid expressing the same pre-fusion stabilized full length transmembrane protein used the pcDNA3.1 backbone and was directly synthesized and cloned into the vector by GeneArt (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... Unmethylated pUC19 was extracted using the Monarch Plasmid Miniprep Kit (NEB, Ipswich, MA). 10 μg of pUC19 was CpG methylated in vitro using 40 units of M.SssI (NEB ...
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bioRxiv - Microbiology 2021Quote: All bacterial two-hybrid plasmids were constructed using the Gibson assembly mix (NEB) or the Hifi DNA assembly mix (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: Linear transformation cassettes were cut out of circular plasmids using EcoRV-HF (NEB) and transformed into wild-type strain T222+ or RBCS1 and RBCS2 double-knockout strains Cal13.1B-[5,46] or T60 [26] ...
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bioRxiv - Neuroscience 2020Quote: This plasmid was generated using NEBuilder HiFi DNA Assembly (New England Biolabs #E5520S), using the following fragments generated by PCR from the indicated template with the indicated primers:
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bioRxiv - Cancer Biology 2021Quote: ... The plasmid was amplified in DH5a cells (NewEngland Biolabs, Ipswich, MA; Cat #C2987H), and purified using plasmid purification midi-kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... the plasmid was linearised by restriction digest using EcoR1 (New England Biolabs (NEB), Herts ...
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bioRxiv - Immunology 2019Quote: ... the plasmid was linearised by restriction digest using EcoR1 (New England Biolabs (NEB), Herts ...
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bioRxiv - Microbiology 2019Quote: ... This plasmid was then digested by XbaI and PstI-HF restriction enzymes (NEB) and the fevR insert was subsequently cloned into pSET151 [41] digested with the same enzymes ...
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bioRxiv - Genetics 2019Quote: The initial plasmid library generated in Step I was digested with BsmBI (NEB) for 9 h and treated with 2 μl of calf intestinal alkaline phosphatase (NEB ...
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bioRxiv - Genetics 2020Quote: ... Another verification was done by digesting plasmids with restriction enzymes XhoI & BsaI (NEB). Finally ...
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bioRxiv - Microbiology 2019Quote: ... plasmids encoding HMPV antigenomes were amplified with Phusion DNA polymerase (New England Biolabs) using primers (listed in Table 1 ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were assembled into an intact plasmid using NEBuilder HiFi mix (NEB). Plasmids were transformed into E ...
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bioRxiv - Synthetic Biology 2020Quote: ... All expression plasmids were generated using standard cloning techniques or Gibson assembly (NEB). All PCR-amplified sequences were verified by DNA sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids (1 ug) were linearized using BamHI restriction enzyme (NEB, Ipswich, MA, USA), column-purified using the DNA Clean & Concentrator −5 Kit (Zymo Research Corporation ...
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bioRxiv - Molecular Biology 2020Quote: The plasmid constructs were generated using Gibson Assembly Master Mix (New England Biolabs). The cDNAs of splicing proteins were PCR amplified and was subcloned into pGEX4T1 vector ...
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bioRxiv - Genetics 2019Quote: ... Gibson assembly of plasmids was conducted with Assembly Master Mix (New England Biolabs), and plasmids were transformed into JM109 competent cells (Zymo Research) ...
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bioRxiv - Genetics 2020Quote: ... the pU6-pegRNA-GG-acceptor plasmid was digested overnight using BsaI-HFv2 (NEB), loaded on a gel and the 2.2kb band was extracted using the QIAquick Gel extraction kit ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids expressing mgrB variants were constructed using Q5 site-directed mutagenesis kit (NEB). All constructs were confirmed by DNA sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were linearized with 0.25 μL of NotI-HF (NEB, Ipswitch, MA, USA) for 30 min at 37°C in a 20 μL reaction at 1 × 1010 copies/μL ...
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bioRxiv - Microbiology 2021Quote: ... Sequence-verified plasmids were transformed into chemically competent NiCo21(DE3) cells (NEB, #C2529H) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The resulting plasmid lentiMPRA library was electroporated into 10-beta competent cells (NEB) using a Gemini X2 electroporation system (BTX ...
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bioRxiv - Immunology 2020Quote: ... the plasmid was cut with EcoRI and BamHI restriction enzymes (NEB, Ipswich, MA), and BiTE cDNA excised from the pAmp vector was directly ligated with T4DNA ligase ...
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bioRxiv - Microbiology 2020Quote: ... The positive plasmids were linearized using SpeI restriction enzyme (New England Biolabs, UK) and the concentrations were measured using dsDNA high sensitivity assay kit in Qubit 3.0 fluorometer (Thermofisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... the plasmid was ligated by T4 ligase (New England Biolabs, cat# 101228-176) and transformed into Agilent XL1-Blue Electroporation-Competent Cells (Agilent ...
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bioRxiv - Genetics 2020Quote: ... This intermediate plasmid product (pLCv2-GFP) was digested with AfeI (NEB, Cat# R0652S) and EcoRI-HF (NEB ...
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bioRxiv - Microbiology 2021Quote: ... plasmid DNA and PCR amplicons were digested with commercial restriction enzymes (NEB, USA); DNAs were ligated using T4 DNA ligase (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were then digested with AflIII and XbaI (both NEW ENGLAND BIOLABS) to cut out the hU6 promoter and sgRNA sequence ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid and insert products were digested using appropriate restriction enzymes (New England Biolabs) and ligated using T4 Ligase (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR fragment and plasmid were digested with AscI and NheI (New England Biolabs). Because 4mt-GCaMP6f contains an NheI digestion site ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting Attl1-mCMV-Kozak-ATRX-Attl2 plasmid was digested using KasI (NEB) and the PB-TAC-ERN plasmid (a gift from Knut Woltjen ...
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bioRxiv - Developmental Biology 2022Quote: ... digested pCS2 plasmid (Lawson #444) with NEBuilder HiFi DNA Assembly Master Mix (NEB). The assembled plasmids were used for the transformation of chemically competent E ...
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bioRxiv - Plant Biology 2022Quote: ... we linearized plasmid pL4440-CP in independent reactions with BglII and HindIII (NEB). Once linearized ...
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bioRxiv - Plant Biology 2022Quote: ... After blunting the plasmid ends with Klenow enzyme (New England Biolabs, Hitchin, UK), the plasmid was religated with the T4 DNA Ligase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... All plasmids generated were assembled using Gibson Assembly Master Mix (New England Biolabs) per manufacturer protocols ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid vectors were then digested with KpnI-HiFi and XbaI (New England BioLabs) at 37°C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: A circularizable LY2 plasmid was digested with Esp3I (New England Biolabs catalog # R0734) and PvuI-HF (New England Biolabs catalog # R3150) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids (Table S3) were propagated in Escherichia coli DH5α cells (NEB, United Kingdom) by growing cells in LB media without NaCl (Formedium ...
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bioRxiv - Neuroscience 2022Quote: ... while the Ift52 plasmid was linearized with KpnI or SacI (New England Biolabs), for the synthesis of sense and antisense single-stranded RNAs (ssRNA ...
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bioRxiv - Plant Biology 2022Quote: ... Of each entry plasmid 100 ng was combined with 1× Cutsmart buffer (NEB), 1 mM ATP ...
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bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were treated with PreCR Repair Mix (M0309, New England Biolabs) and purified with the Monarch PCR & DNA Cleanup Kit (T1030 ...