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Citations for New England Biolabs :
7851 - 7900 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared following the protocol from NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7770S) and indexes (NEB #E7335S and #E7500S).
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Immunology 2020Quote: ... The mAbs were reverted to the UCA sequence in a step-by-step manner using the Q5® Site-Directed Mutagenesis Kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were cloned and performed Gibson assembly together with the triple-digested vector pCVD443 (NEBuilder HiFi DNA Assembly Cloning Kit E5520S, New England BioLabs Inc.). Assembly mixture was then used to transformed E ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA obtained from the ChIP-assays was adaptor-ligated and amplified using the NEBNext multiplex oligo kit (New England BioLabs, E7335S) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Library preparation was done using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs, Frankfurt, Germany) and the protocol for use with inputs of ≥ 100 ng DNA ...
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bioRxiv - Biochemistry 2020Quote: Mutagenesis of the 5-HT2C construct was performed according to the Q5® site-Directed Mutagenesis Kit protocol (New England BioLabs). In brief ...
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bioRxiv - Genomics 2019Quote: RNA from P2 and P9 hypothalamus and cortex was prepared for RNA-seq library using the NEBNext rRNA Depletion kit (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S) ...
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bioRxiv - Plant Biology 2019Quote: ... Library preparation was carried out according to manufacturers instructions (NEB Next Ultra DNA Library Prep Kit for Illumina, New England BioLabs Inc) and paired end sequencing was performed on the Illumina platform at the Cold Spring Harbor laboratory (New York ...
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bioRxiv - Microbiology 2019Quote: ... plus 280 nM each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Immunology 2019Quote: ... and libraries were prepared from 2 ng of total RNA using the NEBNext low input kit (New England Biolabs, Hitchen, U.K.). Libraries were assessed for correct size distribution on the Agilent 2200 TapeStation and quantified by Qubit DNA High Sensitivity assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The Gag-PYQE-mCherry plasmid was modified by site-directed mutagenesis to make a base pair substitution mutation (C to A) that changes the PYQE motif to PYKE using the Q5 SDM kit (New England Biolabs, USA). Gag-PYQE-mCherry plasmid was linearized by PCR using 5’-AAGGAGCCTCTGACGAGCC-3’ as forward primer (with the C to A base pair substitution underlined ...
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bioRxiv - Microbiology 2019Quote: ... These PCR products were assembled together either by sticky-end ligation of restriction-cut PCR products or by Gibson Assembly using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, USA).
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bioRxiv - Genomics 2021Quote: ... cDNA synthesis by RT was performed immediately after DNaseI digestion of placental RNA with the ProtoScript®II First Strand cDNA Synthesis Kit (E6560, New England Biolabs) and random hexamer primers ...
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bioRxiv - Genomics 2021Quote: ... 250–500 ng extracted RNA was used to prepare libraries with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs #E7760S) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed using 1ug DNA-free total RNA with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA. NEB#E7770). Poly (A ...
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bioRxiv - Genomics 2021Quote: ... 1μg of total RNA per sample was processed using the NEBNext Ultra II Directional RNA library prep kit from NEB (E7760L) utilising the NEBNext Poly(A ...
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bioRxiv - Genomics 2021Quote: ... were used as template of the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit; #E2050S, New England Biolabs), performed at 37°C for 16h ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA libraries were prepared from total RNA samples with an RIN ≥ 8.0 using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England BioLabs). An Illumina NovaSeq 6000 machine with an S4 flow cell was used to generate pairwise 100 bp reads (performed by the Yale Center for Genome Analysis).
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bioRxiv - Genetics 2020Quote: ... All DNA fragments were assembled using the NEBuilder® HiFi DNA Assembly Cloning Kit (Catalog number: E5520S, New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... An Illumina DNA library was prepared with the NEBNext® Ultra™ II FS DNA Library Prep Kit (NEB, Ipswich, USA) and multiplex barcoded with NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2 ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were purifified using the FastPure Gel DNA Extraction Mini Kit (Vazyme, DC301-01, Nanjing, China) and then digested by BamHI and HindIII restriction enzymes (New England Biolabs, Inc.). C-terminal EGFP-tagged mouse full-length Pabpn1l was cloned into pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2021Quote: ... containing TauRD-I260C for fluorescent labeling were constructed by mutagenesis of the pHUE-TauRD (P301L/V337M) plasmid using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Cell Biology 2021Quote: ... RNA-seq libraries were generated with 300 ng of total RNA using NEB Next Ultra II RNA Library prep kit (NEB, E7770) according to the vendor’s protocol ...
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bioRxiv - Genomics 2021Quote: We generated three replicates of +/- doxycycline-treated RNA-sequencing libraries with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7765S). We quantified and assessed the quality of the libraries with an Agilent 2200 Tapestation instrument ...
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bioRxiv - Microbiology 2021Quote: ... OtownIsak and SummerBoyErik were prepared using NEBNext® Ultra II FS library prep kit for Illumina (New England Biolabs, Ipswich, USA), while the sequencing library for Hovsa was prepared with Nextera® XT DNA Kit (Illumina ...
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bioRxiv - Immunology 2021Quote: ... The D614G Spike was generated by introducing the corresponding mutation in the Wuhan reference strain using Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Physiology 2020Quote: ... Mutagenesis of Ser125 to Arg in GPT and Ser153 to Arg in GPT2 17 was performed with the Q5 site-directed mutagenesis kit from NEB (#E0554). Adeno-associated viruses (AAV ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragments with the size of 350-700 bp were separated on an agarose gel during electrophoresis and purified for input into the NEBNext Ultra DNA Library Prep Kit for Illumina® (New England BioLabs). Prepared libraries were quantified using KAPA Library Quantification Kits for Illumina® platforms (KAPA Biosystems ...
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bioRxiv - Genomics 2020Quote: ... where a sequencing library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA). This library was sequenced on an Illumina platform with 150 bp paired-end chemistry.
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bioRxiv - Cell Biology 2020Quote: ... Libraries for next generation sequencing were constructed with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) and sequenced on a HiSeq2500 Sequencer ...
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bioRxiv - Cell Biology 2020Quote: Three point mutations in the predicted miR-145 seed binding site in DUSP6 were introduced in pGEM-T-DUSP6 3’UTR using a Phusion® site-directed mutagenesis kit (NEB) and the mutagenic primers ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/μL was used for subsequent reverse transcription assay with Luna® Universal One Step RT-qPCR kit (E3005, New England Biolabs) according manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Yeast Coq7 and Coq9 point mutants were constructed as described in the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) and were confirmed via Sanger sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... Transcription templates were amplified from the plasmids by PCR and and transcribed in vitro with the HiScribe T7 High Yield RNA synthesis kit (New England Biolabs Inc.). To obtain sufficient amounts of mRNA ...
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bioRxiv - Biochemistry 2021Quote: Population genomic DNA was extracted from frozen cell pellets using the Monarch Genomic DNA Purification Kit (New England Biolabs, Beverly, MA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA was isolated from parental and KDM6B KO2 HeLa cells and subjected to bisulfite modification by EpiMark® Bisulfite Conversion Kit according to the manufacturer’s instructions (New England Biolabs, E3318S). The converted DNA was eluted in DNase/RNase-free water and examined by PCR using SYBR Green PCR Master Mix (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... and libraries were generated for next-generation sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760L) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: The predicted late domain motifs encoded in TgGRA14 C-terminus were mutated in the pGagGRA14_Venus by site-directed mutagenesis (Q5 Site-Directed mutagenesis kit NEB, Cat#E0554S). Primers encoding mutation alanine substitutions for PTAP or YPXL were used to generate pGagGRA14TSG101-_Venus and pGagGRA14ALIX-_Venus (P11 + P12 and P13 + P14) ...
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bioRxiv - Developmental Biology 2021Quote: ... IRES-Puro and an FRT-flanked PGK-hygromycin selection cassette to generate the targeting vector using the NEBuilder High-Fidelity DNA Assembly Cloning Kit (NEB, E5520). Twenty-one bp downstream of the start codon ...
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bioRxiv - Synthetic Biology 2021Quote: Expression units were assembled from cloned level 0 parts into level 1 destination vectors provided by the MoClo kit using 40fmol of each vector and 20 U of BsaI-HFv2 (NEB, R3733L) with remaining conditions as described above ...
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bioRxiv - Immunology 2021Quote: ... Twenty ng of purified PCR product was used as input for library construction using the NEBNext Ultra II FS DNA Library Prep Kit (NEB, E6177S) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription reactions were performed with 6 μl of purified RNA and random primers using the NEB ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and all mutant forms of NMA1 were created using a Q5 site-directed mutagenesis kit (New England Biolabs, Ispwich, MA, USA).
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bioRxiv - Neuroscience 2021Quote: ... We amplified and prepared these libraries for sequencing with the NEB Next Ultra RNA Library Prep Kit (New England Biolabs E7530S) and NEBNext multiplex oligos for Illumina (NEB E7335S) ...
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bioRxiv - Molecular Biology 2021Quote: ... Klenow-mediated addition of an adenine to the 3’ end of the DNA fragments was performed using the Klenow fragment (3’→5’ exo-) kit (NEB, M0212L) by combining the 32 μl sample with 5 μl 10X Klenow Buffer NEB 2 ...
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bioRxiv - Genomics 2021Quote: ... and libraries were generated from 250 ng starting material with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs E7765). Libraries were sequenced on an Illumina NextSeq 500 with 150 bp paired-end reads.
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bioRxiv - Genomics 2022Quote: ... 100 ng of the resulting 5C product was prepared for sequencing on the Illumina NextSeq 500 using the NEBNext Ultra DNA Library Prep Kit (NEB E7370) following the manufacturer’s instructions with the following parameter selections ...
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bioRxiv - Pathology 2021Quote: ... in an Applied Biosystems StepOnePlus Real-Time PCR System (USA) and tracked with a DNA-intercalant green fluorophore provided with the WarmStart LAMP Kit (NEB, USA). The RT-LAMP amplification was set to 45 minutes for experiments illustrated in Figures 2 ...