Labshake search
Citations for New England Biolabs :
7701 - 7750 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... to lysine (K) at 363 (E363K) mutant of HNF4A2 was generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The pcDNA3-SHP-Flag vector for SHP was synthesized by GenScript ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified by extraction from a 1% agarose gel using the Monarch DNA Gel Extraction Kit following manufacturer instructions (NEB # T1020S). The partially degenerate DNA oligonucleotide ...
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bioRxiv - Microbiology 2020Quote: ... and libraries for RNAseq were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The 5’ arm and LexA:p65 coding sequence was assembled from 2 fragments from PCR-amplified Drosophila genome and a LexA:p65 coding sequence using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, cat#E5520), and inserted into XhoI-SpeI sites of the pHD-DsRed plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences) using the Luna Universal qPCR kit (New England Biolabs, M3003). Relative expression was normalized to Actb and Gapdh housekeeping genes and was determined using the ΔΔCt method ...
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bioRxiv - Cancer Biology 2020Quote: ... The GST tag in this construct was replaced by a 6xHis tag using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). N-PTEN was recombinantly expressed in E ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng total RNA per 20 µl reaction were analyzed using the Luna Universal Probe One-Step RT-qPCR Kit (NEB, # E3006) in technical triplicates ...
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bioRxiv - Cell Biology 2020Quote: ... The pPISD-LD-eGFP vector was used as substrate to generate different mutants of PISD-LD by Q5® Site-Directed Mutagenesis kit (NEB). All final PCR-generated DNAs were confirmed by DNA sequencing ...
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bioRxiv - Genetics 2021Quote: ... cDNA was generated from 1ug of total RNA using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, E6560). RT-qPCR analysis was performed in Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... The pair-end sequencing libraries with ∼300 bp insert size were constructed following the instructions of NEB Next Ultra DNA Library Prep Kit for Illumina (E7370, New England Biolabs, USA). Illumina HiSeq 2500 systems were used for sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB, USA). Sequencing was performed using a NextSeq HIGH 150 (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: Libraries were prepared using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs). Quality control and quantification of the libraries were performed using the Bioanalyzer DNA 1000 kit (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... was used per sample for the library preparation using the NEB Next Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7760L). The libraries were then sequenced on Illumina HiSeq 2500 sequencing platform using 2×100 bp sequencing format.
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bioRxiv - Biochemistry 2022Quote: ... UPF2L (120-1227) were produced by PCR amplification (primers listed in Supplementary Table S1) using the Phusion High-Fidelity PCR kit (NEB #E0553S) prior to restriction digest and ligation into pPROEX-HTB (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Y160D+Y167D and Avi-tagged Avi-UPF2-MIF4GIII as well as Avi-UPF3B-WT constructs were generated using a Q5 Site-Directed Mutagenesis Kit (NEB #E0554S) with mutagenic primers (Eurofins Genomics ...
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bioRxiv - Molecular Biology 2022Quote: The rest of RNAs were performed with real-time reverse transcription PCR (RT-qPCR) using a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s recommendations by a thermocycler (BIO-RAD CFX96™ Real-Time System) ...
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bioRxiv - Genomics 2022Quote: ... The resulting polyA-selected RNA was used to prepare RNA-seq libraries using the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (cat. #E7420L, New England Biolabs), NEBNext Multiplex Oligos for Illumina® Dual Index Primers (cat ...
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bioRxiv - Cell Biology 2022Quote: RNA isolation was performed starting with a confluent 10cm dish and using the Monarch total RNA miniprep kit for RNA isolation (NEB, #T2010S). RNA was stored at −80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Piggybac vectors containing FLAG-BAF WT and FLAG BAF A12T were subsequently assembled by PCR amplification followed by Gibson assembly using HiFi DNA assembly cloning kit (New England Biolabs, #E5520S). FLAG-BAF was cloned into a BamHI and AfeI digested piggybacV1_CMV ...
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bioRxiv - Molecular Biology 2022Quote: ... all of the eluted DNA was used to construct NextSeq DNA libraries using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, #E7645S). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (New England Biolabs (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... The library was cloned into a pENTR1A plasmid backbone with a C-terminal YFP HA reporter tag using Gibson Assembly (HiFi DNA Assembly Cloning Kit, New England Biolabs, (NEB), Ipswich ...
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Cancer Biology 2022Quote: ... Fragmented DNA was end-repaired and adapter ligation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA) for Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... The genomic DNA was subjected to a 5-hmC-specific enrichment with EpiMark 5-hmC and 5-mC analysis kit (New England Biolabs, USA). The processed DNA samples were analyzed with qPCR using loci-specific primers (Sf 4) ...
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bioRxiv - Molecular Biology 2022Quote: ... strand specific RNA-seq libraries were generated using NEBNext polyA selection and Ultra Directional RNA library preparation kits (NEB, Hertfordshire, UK), multiplexed ...
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bioRxiv - Plant Biology 2022Quote: ... RAPTOR1B full-length cDNA was cloned into pENTR4 and used for generating site-mutated constructs with the Q5 site-directed mutagenesis kit (New England Biolabs, E0554S). RAPTOR1B full-length and site-mutated cDNA were subcloned into binary vector pGWB611 by Gateway LR recombination reaction (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... were prepared using the NEBNext® Ultra II FS DNA Library Prep Kit for Illumina with NEBNext Sample Purification Beads (New England BioLabs). This protocol includes a short PCR amplification (5-7 cycles ...
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bioRxiv - Microbiology 2022Quote: ... All CRISPR/Cas9 plasmids designed and used in this study were created by using Q5 Site-Directed Mutagenesis Kit (NEB-E0554S) based on the previously described CRISPR/Cas9 plasmid [58] as primary scaffold ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 Spike mutant D614G was generated by site-directed mutagenesis using as an input DNA the expression vector encoding SARS-CoV-2 Spike_614D protein (kindly provided by J. Garcia-Arriaza, CNB-CSIC) by Q5 Site Directed Mutagenesis Kit (New England Biolabs, Barcelona, Spain) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Set8RG and Set8RGHL were constructed using site-directed mutagenesis using the Q5 Site-directed Mutagenesis kit on pDEST w+ attB Set8WT (NEB E0554S). KMT5A ...
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bioRxiv - Biochemistry 2022Quote: ... E7490] from 1μg of total RNA for RNA library preparation and sequencing using NEBNext Ultra Directional RNA Library Preparation Kit for Illumina (New England Biolabs, E7420S) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... 50 mL cell culture were grown in LB to stationary phase and processed with the NEB Monarch genomic DNA purification kit (NEB #T3010). For genomic DNA extraction of E ...
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bioRxiv - Microbiology 2022Quote: ... These three PCR products were assembled with a double digested pUC19 (PstI and EcoRI) using a NEBuilder HiFi DNA Assembly kit (New England Biolabs, USA), which resulted in the vectors pΔmucG ...
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bioRxiv - Plant Biology 2022Quote: ... and LCM cell sample collections (ME, TAP, and OSC) was performed using the Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs). Quantitative PCR was performed using TaqMan primers synthesized by Integrated DNA Technologies (Table S2 ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were prepared from 10ung of RNA from each sample using the NEBNext Ultra kit (New England BioLabs, Ipswich, MA, USA). Samples were sequenced on an Illumina HiSeq 4000 with a 75ubp paired-end read ...
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bioRxiv - Neuroscience 2022Quote: ... 100 sorted cells were collected into individual wells of 96-well plate containing 5 μl of lysis buffer of NEB Next single-cell low input RNA library prep kit for Illumina (New England Biolabs #E6420). Plates were frozen immediately on dry ice and stored at −80 °C ...
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bioRxiv - Biochemistry 2022Quote: ... All CC mutants were generated from untagged WT SUN1 and SUN2 constructs by PCR-mediated mutagenesis (NEB site directed mutagenesis kit) (Table S1).
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bioRxiv - Biochemistry 2022Quote: mRNA synthesis via in vitro transcription was performed using linearized plasmid DNA using High Scribe T7 Polymerase HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs): 100 mM NTPs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... apterus libraries were prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, United States) at the Berlin Center for Genomics in Biodiversity Research (BeGenDiv) ...
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bioRxiv - Genetics 2022Quote: ... RNAseq libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs Inc., Ipswich, MA) and sequenced with the Illumina HiSeq instrument (Illumina Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... The paired-end libraries were prepared using a NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA libraries were made using the NEBNext Ultra II Directional RNA library Prep Kit for Illumina using the established protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB#E7490). We used Rapid Run OBCG single read 50 bp on the Illumina Hi-Seq 2500 system to sequence these libraries with 2 technical replicates for each time-point and genotype ...
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bioRxiv - Plant Biology 2021Quote: ... Paired-end libraries of DNA were constructed by the NEBNext Ultra II DNA Library Prep for Illumina Kit (New England Biolabs; E7645L) with 2 × 150 bp with average insert size ~900 bp and sequenced on a NovaSeq 6000 platform (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of the replication of each mutant versus the reference was performed using Luna® Universal Probe One-Step RT-qPCR kit (New England BioLabs) containing 3uL of total RNA ...
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bioRxiv - Developmental Biology 2020Quote: High-throughput sequencing libraries from ChIP and input DNA were prepared using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370S). During library preparation ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA) and index codes were added ...
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bioRxiv - Immunology 2020Quote: ... Purified DNA was subjected to ChIP-Seq library preparation with NEBNext Ultra II DNA Library Prep Kit for Illuumina (NEB, #E7645) and sequenced by paired-end 75-bp sequencing on Next-Seq550 ...
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bioRxiv - Microbiology 2021Quote: ... RNA quality was assessed using the Agilent Bioanalyzer and appropriate samples were used for NGS library preparation with the NEBNext Ultra II Directional RNA kit (NEB E7760) and sequenced with 50 bp paired-end reads and 30 mio reads per sample on the Illumina HiSeq 2500 ...
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bioRxiv - Microbiology 2021Quote: ... Yields of viral RNA were quantified by real-time qPCR by using SARS-CoV-2 specific primers targeting the E gene with the Luna®Universal One-Step RT-qPCR Kit (New England Biolabs) in a LightCycler 480 thermocycler (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... were subcloned into phCMV3 to express C-terminal FLAG-tagged CatSper subunits (phCMV3-CatSperd or z-Flag) using NEBuilder® HiFi DNA Assembly Kit (NEB). A stop codon was placed at the upstream of HA-encoding sequences of phCMV3 vector for FLAG-tagged CatSper subunit cloning.