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Citations for New England Biolabs :
701 - 750 of 1912 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 3 mM MgCl2 and 2 μL (10 U) RNase H (NEB, Cat# M0297S) in order to digest poly(A ...
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bioRxiv - Biochemistry 2024Quote: ... 25 µL Yeast Inorganic Pyrophosphatase (YIPP, 2 kU/mL, New England Biolabs, USA), 50 µg PCR-amplified DNA template ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented by a 2× NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs). Gibson reactions were incubated for one hour at 50°C and then transformed into DH5-alpha competent E ...
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bioRxiv - Genetics 2023Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the YCp50-WT_PKR template ...
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bioRxiv - Microbiology 2023Quote: ... The PCR was performed using Q5 2× Master Mix (New England Biolabs, M0492S), 6 µL of cDNA synthesis reaction ...
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bioRxiv - Genomics 2023Quote: ... 2X hybridisation buffer (4X SSC, 20% dextran sulphate, 2 mg/mL BSA (NEB), 1/10 volume nuclease free water and 1/10 volume vanadyl-ribonucleoside complex (VRC ...
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bioRxiv - Systems Biology 2023Quote: ... then incubated overnight with DraI mix (2 U/uL DraI enzyme (NEB, R0129) in 1X CutSmart buffer ...
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bioRxiv - Systems Biology 2024Quote: ... 2-10 µg plasmid DNA was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 4 h at 37°C and then spin-purified.
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bioRxiv - Physiology 2024Quote: ... The array was loaded with secondary strand synthesis mixture (1X NEBuffer-2 (NEB), 10 uM TruSeq Read2-conjugated Random Primer (IDT) ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA-DNA hybrids were removed with 2 U of RNase H (NEB, M0297S). The synthesized DNA was diluted 1:4 and 1 μl was used for qRT-PCR ...
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bioRxiv - Genomics 2022Quote: ... 25 μl of nuclease free water and 2 μl of DnaseI (M0303S, NEB) were added to degrade the DNA template ...
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bioRxiv - Cancer Biology 2022Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB, Set 1 #E7335S, Set 2 #E7500S). ChIP libraries were done following NEB’s guidelines (NEB ...
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bioRxiv - Genetics 2022Quote: ... and 25 µL of 2× Hot Start High Fidelity Q5 Master Mix (NEB) were performed ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was loaded on four 2 mL chitin columns (NEB, no. S6651S). Columns were washed with 10 mL of HEGX ...
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bioRxiv - Microbiology 2022Quote: ... We used 2× Luna® Universal qPCR Master Mix (New England Biolabs [NEB]) for quantitative PCR in a volume of 20 µL and primer concentrations of 250 nM each for the forward and reverse primers ...
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bioRxiv - Microbiology 2022Quote: ... We used 2× Luna® Universal qPCR Master Mix (New England Biolabs [NEB]) for quantitative PCR in a volume of 20 µL and primer concentrations of 250 nM each for the forward and reverse primers ...
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bioRxiv - Biochemistry 2022Quote: ... followed by addition of 50 μL of extension mix (1X buffer 2 (NEB), 0.4 mM dNTPs ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 μl were combined with 1 μl 10X T4 Ligation Buffer (NEB, M0202), 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Genetics 2022Quote: ... PCRs were performed using Q5 Hot-start 2× master mix (New England Biolabs). Restriction digests were conducted for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...
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bioRxiv - Microbiology 2023Quote: ... 6 ng of sample was treated with 2 units T4 Polynucleotide Kinase (NEB) and incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μl of 10x T4 RNA ligase 2 (truncated) buffer (New England Biolabs), 3 μl of 50% PEG 8,000 (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... the single stranded gRNA oligos were annealed in NEBuffer 2 (New England Biolabs) at 95 °C for 5 min and left to cool ...
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bioRxiv - Genetics 2024Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the MSp509_PKR-WT template DNA ...
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bioRxiv - Genomics 2024Quote: ... at 65 ⁰C for 2 h followed by MspI treatment (NEB Ipswich, MA) at 37⁰C overnight ...
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bioRxiv - Genomics 2024Quote: ... 2 µg of the sgRNA-barcode library was linearized with AscI (NEB, R0558S) and ...
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Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... 10.0 μl NEBNext High-Fidelity 2×PCR Master Mix (New England Biolabs, M0541S), 0.25 μl 100 μM PCR Index 1 ...
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bioRxiv - Genomics 2024Quote: ... and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L) with Illumina Nextera primers and conditions ...
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bioRxiv - Genomics 2024Quote: ... and 21.3 U/µl T4 RNA Ligase 2 Truncated KQ (New England Biolabs) was added to each well and plates were incubated at 4 °C for 18 hours.
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2 and 2 U of recombinant Escherichia coli RNase HI (NEB) were added ...
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bioRxiv - Neuroscience 2024Quote: ... mixed with an equal volume of 2× RNA loading dye (New England Biolabs), was incubated at 75 °C for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs #M0451S) with Nextera DNA CD Indexes (Illumina #20015882) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mg lysates were incubated with magnetic oligo-DT beads (NEB, S1419S) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 and 5 minutes by the addition of 0.8 units Proteinase K (NEB). Fragments were resolved on 1% agarose gel and band intensities were quantified on Image Lab (BioRad).
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bioRxiv - Synthetic Biology 2024Quote: ... 2 µL of 10mM dNTPs (complete mixtures of all dNTPs, New England Biolabs, N0447S ...
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bioRxiv - Genomics 2024Quote: ... also containing a final concentration of 2 mM of each dNTP (N0447S, NEB) and 1X Maxima buffer (EP0752 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... GDP hydrolysis was catalyzed via the addition of 2 units of apyrase (NEB) and a further incubation for 2 h ...
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bioRxiv - Biochemistry 2024Quote: ... and Q5 Hot Start High-Fidelity 2× Master Mix (NEB, catalog no. M0494S) in a 50 µL reaction ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM DTT) and incubated with 1 mg/mL streptavidin (New England Biolabs). The chamber was again washed with TIRF buffer and incubated with 10 μL of a fresh dilution of microtubules (1.5 μL microtubules diluted into 10 μL TIRF-Casein buffer [TIRF buffer supplemented with 50 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... The cleared extract was loaded onto 2 ml pre-equilibrated amylose resin (NEB). The column was washed extensively with amylose buffer (25 mM HEPES-NaOH pH 6.8 ...
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bioRxiv - Bioengineering 2024Quote: ... IVT templates were degraded with 2 µL of DNase I (New England Biolabs) per IVT reaction for 20 min at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... and the NEBNext Multiplex Oligos from Illumina (Index Primers Set 1, 2, 3 and 4, NEB, E7335S, E7500S, E7710S, E7730S), following the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2020Quote: Antibodies were first digested with PNGase F (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... or 5 µl anti-H3K18cr antibody (PTM-517, PTM Biolabs) together with protein A agarose (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... and MBP was detected with MBP antibodies (New England Biolabs).
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bioRxiv - Biochemistry 2023Quote: ... mouse monoclonal antibodies against PAR (clone 10H, Tulip BioLabs, USA), anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μl anti-MBP Monoclonal Antibody (New England Biolabs, #E8032L) and 6 μl anti-E ...
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bioRxiv - Cell Biology 2024Quote: ... anti-MBP antibody from mice (New England Biolabs, 1:3000), Goat anti-mouse IgG HRP conjugated (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... 2 µL of Taq DNA polymerase (LongAmp Taq DNA Polymerase kit, New England Biolabs), 7.5 µL of dNTPs (dNTP set ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEBNext Multiplex Oligos for Illumina Primer sets 1 and 2 (New England Biolabs). The libraries were amplified with 7 PCR cycles using Herculase II Fusion Polymerase kit (Agilent) ...