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Citations for New England Biolabs :
6951 - 7000 of 8151 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Proteins were then degraded by addition of 1 μL of Proteinase K (NEB #P8107) and incubation at 56°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 1X DNase I reaction buffer with 1 µL DNase I (NEB) for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL RNAs were mixed thoroughly with 1 μL T4 PNK (10 U, NEB), 1 μL purified TS2126 and 1 μL PAP1 enzyme (NEB ...
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bioRxiv - Cell Biology 2022Quote: 1 μg of total RNA was reverse transcribed with Protoscript II reverse transcriptase (NEB), both according to the manufacturer’s instructions using random hexamer primers ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... The membrane was incubated with HRP-conjugated (1/2,500 New England Biolabs, Ipswich, MA) or IRDye® (1/10,000 LI-COR Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... The resulting reaction product was mixed with 1 μl Exonuclease V (New England Biolabs) and 3 μl of 10 mM ATP in NEB Buffer 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... were added in a total of 50 µl 1× CutSmart buffer (New England Biolabs). The reaction was run for 15 min at 37 °C on a thermocycler ...
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bioRxiv - Genomics 2023Quote: ... cDNA (1 µg) was prepared from total RNA using LunaScript cDNA synthesis mix (NEB), diluted 5 times before using it for qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... The 385 bp PCR product was digested with 1 unit of Hpy188III (NEB #R0622) for 2 hrs at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Streptavidin conjugated DNA was ligated to the appropriate concentration adapter (1:10) (NEB, 7335L). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... in a reaction mix containing 1× T4 RNA Ligase Buffer (New England Biolabs, M0437M), 1U SUPERase•In RNase inhibitor (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The PacI digest was treated with 1 units T4 DNA polymerase (New England Biolabs) and 2 mM dCTP ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (E7335; NEB) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Both miRNA-encoding plasmids and MG1 shuttle plasmids were digested using MLU-1 (NEB) and pre-miR-30-based cassette inserts were ligated individually into the MG1 shuttle vector ...
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bioRxiv - Developmental Biology 2024Quote: ... The seminiferous tubules were washed twice with 1 U DNAse (NE Biolabs, Ipswich, MA) in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were stopped by adding 2µl of stop buffer (10mg.ml-1 proteinase K (NEB), 1% SDS ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of T4 ligase and 0.5 µL of T4 polymerase (both from NEB) were added to 20 µL of PBS ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using the LUNA 1-step RT-qPCR kit (E3005L, NEB) on an AriaMx Real-time PCR system (G8830A ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mM dNTPs) and 1 µL of ProtoScript® II Reverse Transcriptase (NEB) was added ...
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bioRxiv - Biophysics 2021Quote: ... 25 μL of PCR reaction was mixed with 1 μL of DpnI (NEB, cat#R0176) at 37°C for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin was digested in situ using five units of restriction enzyme CviKI-1 (NEB, R0710S) every 100.000 nuclei and RELACS custom barcodes (4 bp UMI + 8 bp RELACS barcode ...
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bioRxiv - Microbiology 2019Quote: PCR mixtures were digested for 1 h at 37 °C with DpnI (New England Biolabs) to remove the template plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then stained with 1 µM of SNAP-Surface-AlexaFluor 647 (BG-A647, NEB) in 4% BSA + 0.1% Tween-20 for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the dUTP-containing second strand was digested by adding 1 µL of USER enzyme (NEB) and incubating at 37 °C for 15 minutes ...
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bioRxiv - Genomics 2019Quote: ... bead-nuclei were resuspended in 190 µL RNA ligase solution (1× RNA ligase buffer (NEB), 4.5 µM bridge adapter ...
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bioRxiv - Genomics 2019Quote: ... supplemented with 0.1% Triton X-100 and with 200 µL 1× RNA ligase buffer (NEB) by repeating resuspending/pelleting.
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bioRxiv - Cell Biology 2019Quote: ... 1) Genomic DNA was digested with 200U HindIII-HF (New England BioLabs, prod. number R3104S) at 37°C overnight ...
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bioRxiv - Biophysics 2019Quote: ... 25 µL of PCR reaction was mixed with 1 µL of DpnI (NEB, cat# R0176), 1 µL of T4 PNK (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was applied onto a column with 1 ml of equilibrated amylose resin (NEB). The column with the beads and the extract was incubated on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Synthetic Biology 2019Quote: ... We added 1 µL of murine RNase inhibitor to all in vitro reaction (NEB# M0314S). Each reaction also received 60 pmol of either the RED20-encoded or the standard-encoded expression vector ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1 μg of DNA was digested at 37C with the restriction enzyme EcoRI-HF (NEB) for two hours after which MspI (NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... 1× protease inhibitor cocktail) and incubated with 12,000 gel units/ml micrococcal nuclease (NEB, M0247S) at 37 °C with frequently mixing to digest chromatin to lengths of approximately 100-to-1,000 bp ...
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bioRxiv - Genetics 2019Quote: ... Using the 100 bp and 1 kb DNA Quick-Load ladders (N0467 and N0468, NEB) as a reference ...
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bioRxiv - Genetics 2021Quote: ... 1 μl of 10 mM dNTPs and 0.5 μl of OneTaq Polymerase (New England BioLabs). To generate (partially ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Q5 DNA Polymerase (NEB) with primer 8 & 9 or 10 & 11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Taq DNA Polymerase (NEB) with primer 4 & 6 or 5 & 6 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Purified products were ligated with 1 µL T4 DNA ligase (protocol as per NEB®) for 10 minutes at room temperature (benchtop ...
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bioRxiv - Bioengineering 2021Quote: ... for 16 cycles annealing at 67 °C was mixed with 1 μL of ExoI (NEB) digested mixture as the template ...
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bioRxiv - Plant Biology 2021Quote: ... total or virion DNA was treated with 1 unit of Mung Bean nuclease (NEB, Massachusetts). The digestion products were analyzed for SEGS-2 episomes as described above.
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bioRxiv - Genetics 2021Quote: ... 250 ng – 1 μg of RNA samples were digested with Nucleoside Digestion mix (NEB, M069S) for 2 hr at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... PBT was removed and SNAP substrate diluted in PBT (SNAP-Surface649, NEB S9159S; 1:1000) added ...
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bioRxiv - Genomics 2021Quote: ... into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB), 2 U M.CviPI (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and 70 μl water and 1mM MnCl2 added together with 1 μl DNaseI (NEB #M0303S) for 10 minutes at room temperature in order to degrade any DNA that precipitated overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
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bioRxiv - Genetics 2022Quote: ... The supernatant (~12 ml) was added to 1 ml HEGX washed chitin magnetic resin (NEB) and left to bind for 1 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... the library was treated with a 1:20 dilution of USER enzyme (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... at least 1×106 fixed cells were digested using the DpnII restriction enzyme (NEB, R0543M). Chromatin was re-ligated with T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... with ISPCR (IDT; 0.1 μM) and Lambda Exo (New England BioLabs MO262S; 1 units/reaction) for 12 or 14 total cycles.
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bioRxiv - Microbiology 2021Quote: ... we treated the purified RT reaction with 1 μl of Exonuclease I (NEB, Catalog# M0293L) at 37°C for 30 minutes ...