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Citations for New England Biolabs :
651 - 700 of 4584 citations for 6 CHLORO 2 THIOPHEN 2 YL IMIDAZO 1 2 A PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with 2 μl of proteinase K (NEB, 800 units/ml) for 20 min at 37 °C and 5 ul of each sample was applied onto 20% denaturing gels (20% acrylamide/bis-acrylamide ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µg of total RNA was treated with DNase I (New England BioLabs) and then cleaned with RNA Clean and Concentrator-5 (Zymo Research) ...
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bioRxiv - Biochemistry 2021Quote: ... and then digested with 2 μL Nucleoside Digestion Mix (#M0649, New England Biolabs) in a 50 μL reaction incubated at 37 °C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM CaCl2) at room temperature in the presence of recombinant enteropeptidase (NEB) at 13 U enzyme per mg TMPRSS2 zymogen ...
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bioRxiv - Microbiology 2020Quote: ... and 0.15mM of 7-deaza-dGTP (7-deaza-2′-deoxyguanosine 5′-triphosphate, NEB).
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bioRxiv - Immunology 2020Quote: 2 μg of base expression vectors were digested using NotI-HF (NEB # R3189S) and SmaI (NEB # R0141S ...
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bioRxiv - Genomics 2020Quote: ... 10 µL of 2% bovine serum albumin (New England Biolabs, cat. no. B9000S), 0.3 µL of 1M spermine (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... 25 μl of nuclease free water and 2 μl of DnaseI (M0303S, NEB) were added to degrade the DNA template ...
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bioRxiv - Genetics 2022Quote: ... and 25 µL of 2× Hot Start High Fidelity Q5 Master Mix (NEB) were performed ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was loaded on four 2 mL chitin columns (NEB, no. S6651S). Columns were washed with 10 mL of HEGX ...
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bioRxiv - Microbiology 2022Quote: ... We used 2× Luna® Universal qPCR Master Mix (New England Biolabs [NEB]) for quantitative PCR in a volume of 20 µL and primer concentrations of 250 nM each for the forward and reverse primers ...
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bioRxiv - Microbiology 2022Quote: ... We used 2× Luna® Universal qPCR Master Mix (New England Biolabs [NEB]) for quantitative PCR in a volume of 20 µL and primer concentrations of 250 nM each for the forward and reverse primers ...
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bioRxiv - Genomics 2022Quote: ... and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L) with Illumina Nextera primers and conditions ...
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bioRxiv - Synthetic Biology 2022Quote: ... for assembly into levels 2 in 20 μl 1x T4 ligase buffer (NEB). The reaction condition was ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 mM MgCl2 and 2 μL (10 U) RNase H (NEB, Cat# M0297S) in order to digest poly(A ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR mix consists of 2 µL LongAmp Taq DNA Polymerase (NEB, # M0323L), 0.5 µM of each primer (ncec_pcr_fw_v7 ...
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bioRxiv - Genetics 2023Quote: ... Amplification was performed with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541s) and Nextera PCR Primers (8 cycles) ...
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bioRxiv - Biophysics 2023Quote: ... 2 μM X4L4 or 8 U/μL T4 DNA ligase (New England BioLabs) and an increasing amount of PEG 8k (w/v ...
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bioRxiv - Biophysics 2023Quote: ... following incubation for 2 h of 10 mg/mL BSA (New England Biolabs) diluted in Buffer A containing 20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μl NTP buffer mix (1.34 mM final concentration for each NTP, NEB), 2 μl T7 RNA Polymerase Mix (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... followed by addition of 50 μL of extension mix (1X buffer 2 (NEB), 0.4 mM dNTPs ...
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bioRxiv - Genetics 2022Quote: ... PCRs were performed using Q5 Hot-start 2× master mix (New England Biolabs). Restriction digests were conducted for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA-DNA hybrids were removed with 2 U of RNase H (NEB, M0297S). The synthesized DNA was diluted 1:4 and 1 μl was used for qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... The PCR was performed using Q5 2× Master Mix (New England Biolabs, M0492S), 6 µL of cDNA synthesis reaction ...
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bioRxiv - Genomics 2023Quote: ... 2X hybridisation buffer (4X SSC, 20% dextran sulphate, 2 mg/mL BSA (NEB), 1/10 volume nuclease free water and 1/10 volume vanadyl-ribonucleoside complex (VRC ...
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bioRxiv - Developmental Biology 2023Quote: ... 10.0 μl NEBNext High-Fidelity 2×PCR Master Mix (New England Biolabs, M0541S), 0.25 μl 100 μM PCR Index 1 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented by a 2× NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs). Gibson reactions were incubated for one hour at 50°C and then transformed into DH5-alpha competent E ...
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bioRxiv - Genomics 2024Quote: ... and 2 U/µl Thermolabile Exonuclease I (New England Biolabs, catalog no. M0568S) and incubated for 15 min at 37 °C on a shaking thermoblock at 400 rpm ...
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bioRxiv - Systems Biology 2024Quote: ... 2-10 µg plasmid DNA was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 4 h at 37°C and then spin-purified.
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bioRxiv - Systems Biology 2023Quote: ... then incubated overnight with DraI mix (2 U/uL DraI enzyme (NEB, R0129) in 1X CutSmart buffer ...
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bioRxiv - Physiology 2024Quote: ... The array was loaded with secondary strand synthesis mixture (1X NEBuffer-2 (NEB), 10 uM TruSeq Read2-conjugated Random Primer (IDT) ...
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bioRxiv - Biochemistry 2024Quote: ... 25 µL Yeast Inorganic Pyrophosphatase (YIPP, 2 kU/mL, New England Biolabs, USA), 50 µg PCR-amplified DNA template ...
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bioRxiv - Immunology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). The Hifi assembly products were Ampure bead purified and eluted into 20 µL of H2O ...
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Molecular Biology 2020Quote: ... then blunted overnight at 37°C in 100 μl NEBuffer 2 with 0.1 mM dNTPs and 1 μl Klenow (NEB M0210S). After rinsing twice with 1 ml tris buffer ...
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bioRxiv - Genomics 2019Quote: ... Bead-nuclei were pelleted for 2 min at 2500 g and resuspended in 189 µL blunting solution (1× T4 DNA ligase buffer (NEB), 0.25 mM dNTPs ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 µl of the insert was treated with 2 µl Dpn1 enzyme in the presence of 1 µl Cutsmart buffer (10×) (New England BioLabs) for 2 h at 37°C ...
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bioRxiv - Biophysics 2019Quote: ... were bound to the beads in the presence of 1 µM DNA oligonucleotide 5’-TCTCCTCCGAAGAAA-3’ (targeting DNA) and 2 µL RNase H (5 units/µL, NEB) were added to the reaction ...
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bioRxiv - Biochemistry 2020Quote: ... the nonstop GFP1-10 sequence was produced by PCR using primers #1 and 2 and Q5 High-Fidelity DNA Polymerase (NEB) with pETGFP 1-10 vector as a template (Cabantous et al. ...
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bioRxiv - Cell Biology 2021Quote: ... and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC; Primer 2 - 5’-CAAGCAGAAGACGGCATACGAGAT) and Phusion High-Fidelity DNA Polymerase (NEB) and further purified with AMPure XP beads to eliminate unligated primers and adapters ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were amplified by PCR using NextFlex PCR primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC; Primer 2 - 5’-CAAGCAGAAGACGGCATACGAGAT) and Phusion High-Fidelity DNA Polymerase (NEB) before three further rounds of AMPure XP purification were performed to collect fragments 150-300 bp in size ...
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bioRxiv - Plant Biology 2020Quote: ... 17 μl of the PCR reaction were mixed with 2 μl of CutSmart Buffer and 1 μl of AvaI (NEB) for a final volume of 20 μl ...
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bioRxiv - Developmental Biology 2021Quote: ... Pmex-5::PH-GFP-cyk-1(700-1437)::tbb-2 3’UTR in the pCFJ150 backbone [89] was made using HiFi cloning (New England Biolabs). Notably ...
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bioRxiv - Synthetic Biology 2022Quote: ... αlvus tRNAPyl (Ma-tRNAPyl)35 was prepared by annealing and extending the ssDNA oligonucleotides Ma-PylT-F and Ma-PylT-R (2 mM, Supplementary Table 1) using OneTaq 2x Master Mix (NEB). The annealing and extension used the following protocol on a thermocycler (BioRad C1000 Touch™) ...
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bioRxiv - Biochemistry 2022Quote: ... D-loops were deproteinized by adding 2 μL of 5% lithium dodecyl sulfate and 1 μL of 20 mg/mL proteinase K (New England Biolabs), and incubating the mixture at 37°C for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... To seventeen microliters of the eluates were added 2 μL of 10x Nucleoside Digestion Mix reaction buffer and 1 μL of Nucleoside Digestion Mix (New England Biolabs). Reactions were incubated at 37 °C for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... The agarose was digested by 1 hour incubation at 42 °C with 2 units of beta-agarase (M0392, New England Biolabs). After this stage ...
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant was removed using a magnetic separator and washed once with 1 mL NEBuffer 2 and resuspended in 115 mL of Ligation reaction with Quick Ligase buffer (NEB), 6,000 U of Quick Ligase (NEB ...
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bioRxiv - Biochemistry 2020Quote: pTXB1 Halo 3C SARS-CoV-2 (2019-nCoV) Nsp1 1-179-Intein CBD (DU67780 was made using NEBuilder (New England Biolabs), amplifying the vector from existing clone DU28033 (pTXB1-HALO-Mxe Intein-CBD ...