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Citations for New England Biolabs :
651 - 700 of 3716 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Then 3 µl USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Genomics 2023Quote: ... 3 µl of Lambda Exonuclease (NEB, #M0262L), and 3 µl of Exonuclease I (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... Then 3 μl USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Biochemistry 2024Quote: ... Then 3 µL USER Enzyme (NEB, USA) was used with size selected ...
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bioRxiv - Cancer Biology 2024Quote: ... Then 3 μl USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Systems Biology 2024Quote: ... then 3 μL of USER enzyme (NEB) was added and mixed again by pipetting ...
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bioRxiv - Genomics 2024Quote: ... 3 μl each of HinP1I (NEB R0124S), DdeI (NEB R0175L) ...
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bioRxiv - Cell Biology 2024Quote: ... Then 3 ml USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Genomics 2024Quote: ... and 2 µL 10x NEBuffer 3 (NEB) in a total volume of 20 µL and was incubated for 40 min at 37 °C and 60 min at 45 °C ...
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bioRxiv - Genomics 2024Quote: ... and 3 µL 10x ThermoPol buffer (NEB) were added for final volume of 30 µL ...
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bioRxiv - Genomics 2024Quote: ... and 3 µL 10x NEBuffer 2 (NEB) were added for a final volume of 30 µL ...
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bioRxiv - Genetics 2024Quote: ... 3 µL of rCutSmart Buffer (NEB, B6004S) and 3 µL nuclease-free water were added before the Cas9 digestion ...
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bioRxiv - Bioengineering 2024Quote: ... 3 μL 10X rCutSmart buffer (NEB, B6004S), H2O to 30 μL incubated at 37°C for 10 min and heat inactivated at 80°C for 2 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Then 3 µl USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Cell Biology 2024Quote: ... Ligation reactions were quenched by addition of EDTA to 25 mM and proteins were digested by incubation at 37°C for 20 min with 0.1% SDS and 20 units/ml proteinase K (NEB). Unligated oligonucleotides were removed by gel filtration through a 50 cm x 0.7 cm Sepharose 4B column (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... The genomic DNA was subjected to a 5-hmC-specific enrichment with EpiMark 5-hmC and 5-mC analysis kit (New England Biolabs, USA). The processed DNA samples were analyzed with qPCR using loci-specific primers (Sf 4) ...
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bioRxiv - Molecular Biology 2021Quote: ... and to a 5’ adapter (5’-GUUCAGAGUUCUACAGUCCGACGAUC) with T4 RNA ligase I (NEB). The resultant RNA was reverse-transcribed to cDNA with Superscript III (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’ RNA ligation was performed using 0.5 μl 5’ SR Adapater (NEB-kit) instead of the 5P RNA oligo.
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bioRxiv - Genomics 2022Quote: ... and followed by a 5′ decapping with RNA 5′ pyrophosphohydrolase (RppH, NEB, M0356S). The 5′ end was phosphorylated using T4 polynucleotide kinase (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... and the m7G(5’)ppp(5’)G RNA Cap Structure Analog (#S1411, NEB) kits ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of an m7G(5⍰)ppp(5⍰])G RNA cap (NEB). Transcription was carried out at 42°C for 2 hours (h ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of nuclease-free water and 5 µL of GA mastermix (NEB) were added and incubated at 40°C for a minimum of 1.5 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 mM G(5’)ppp(5’)G RNA Cap Analogue (New England Biolabs), 4 μg DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Ligation of 5’ adaptor required (i) Removal of the 5’ cap using RNA 5’ pyrophosphohydrolase (RppH, New England Biolabs, Ipswich, MA, M0356S) (ii ...
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bioRxiv - Molecular Biology 2020Quote: Oligonucleotide was annealed before use: 20 μl 100 pM/μl oligonucleotide and 20 μl 10x T4 DNA ligase buffer (NEB) in 200 μl final volume were incubated in a heating block 95°C 5 min then block was removed from heat and left to cool to room temperature over ~2 h.
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bioRxiv - Molecular Biology 2020Quote: ... were separately subjected to restriction enzyme double digestion at 37 °C overnight in 20 μL volumes containing 20 U BamHI-HF (cat. no. R3136S, New England BioLabs), 20 U EcoRI-HF (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... We assembled a 20 ng amplified cassette into a 50 ng digested vector in a 20 μL Gibson reaction (NEB). The individual construct was verified using Sanger sequencing (Genewiz).
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bioRxiv - Biophysics 2023Quote: ... two identical Golden Gate Assembly reactions were carried out by mixing 7 μl of each ∼ 20 nM DT4 plasmid and ∼ 20 nM ligator fragment with 1 μl of Golden Gate Mix (New England Biolabs) in T4 DNA Ligase Buffer (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... One micro litter of circularized cDNA was subjected to PCR in a 20 μL reaction mixture composed of 20 U/mL Phusion polymerase (NEB), 1x Phusion GC buffer (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... 20 units of T4 polynucleotide kinase (NEB, M0201) and water to a total volume of 20 µl ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 U/μL HinfI enzyme (NEB, Cat #R0155), x1 Exonuclease I Reaction Buffer (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μL 25 mM dNTPs (NEB, Cat #N0446S), 30 μL 0.1 M DTT (SuperScript™ III Reverse Transcriptase Kit ...
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bioRxiv - Systems Biology 2020Quote: ... 20 μL T7 DNA ligase reaction buffer (NEB), and 2 μL nuclease-free water then incubating at 37°C overnight ...
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bioRxiv - Genetics 2020Quote: ... 6 μl 20 mg/ml BSA (NEB B9000S), and 5 μl of 400 U/μl T4 ligase (NEB M0202S/L) ...
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bioRxiv - Molecular Biology 2022Quote: ... or 20 U of BamHI (New England Biolabs) for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... and 20 U of Klenow fragment exo- (NEB). The A-tailing reaction was incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... and then PCR amplified for 20 cycles (NEB Phusion Master Mix ...
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bioRxiv - Genomics 2020Quote: ... 20 U of T4-βGT (NEB, Ipswich, MA) and 1 μl of 50x UDP-glucose were added and incubated for 30 min at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 20 units of calf alkaline phosphatase (NEB) were added to the cooled solution and incubated at 37°C for 1 hour ...
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bioRxiv - Genomics 2020Quote: ... 10 µ of 20 mg/mL BSA (NEB), and 950 µ of nuclease-free water ...
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bioRxiv - Genomics 2022Quote: ... 1% (v/v) BSA (20 mg/ml, NEB)] + 5μL of hash oligo (10 uM ...
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bioRxiv - Immunology 2022Quote: ... and 20 µg/ml tetanus toxoid (CreativeTM Biolabs) (as positive controls).
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bioRxiv - Molecular Biology 2023Quote: ... 20 U T4 DNA ligase enzyme (NEB, USA) and milliQ water for 1 hour at 16 °C which was followed by enzyme inactivation for 20 mins at 80 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 µL BsaI-HF (20 U/µL; NEB), 0.5 µL T4 Ligase (400 U/µL ...
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bioRxiv - Microbiology 2023Quote: ... 20 U murine RNase inhibitor (New England Biolabs) were added to each reaction ...
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bioRxiv - Biochemistry 2023Quote: ... in 20 µl of T4 ligase buffer (NEB) for 1 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μl SNAP-Capture beads (NEB, S9145S) were pre-equilibrated in 150 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... with 20 U of DNase I (NEB, M0303S) for 1mL of phage lysate ...
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bioRxiv - Genetics 2023Quote: ... We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles, Tm 65 °C). We then performed a bead cleanup ...
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bioRxiv - Microbiology 2024Quote: ... 20 U of DpnI (New England Biolabs : R0176S) was added to the PCR products and incubated at 37 °C for 1 hour to remove the parental PCR DNA template ...