Labshake search
Citations for New England Biolabs :
6901 - 6950 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... We added Illumina TruSeq adaptors using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated by deletion of the unwanted DNA from the parental plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB BioLabs). The R763G point mutation was introduced into D1-A1 by PCR mutagenesis ...
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Characterization of novel Actinobacteriophage Giantsbane reveals unexpected cluster AU relationshipsbioRxiv - Genomics 2022Quote: ... Sequencing libraries were made using a NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs, MA, USA) and were sequenced using the Illumina MiSeq platform to 2197x coverage ...
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bioRxiv - Genomics 2022Quote: ... Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). All the libraries were checked through a Bio-analyzer for quality control purpose ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A donor DNA containing two homology arms flanking the DsRed sequence for homology repair were cloned into the pHD-ScarlessDsRed plasmid (https://flycrispr.org/scarless-gene-editing/) using the Q5® High-Fidelity DNA Polymerase kit (New England Biolabs). Left homology arm contained the sequence in 2R:9870299-9871095 (FlyBase Release 6 ...
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bioRxiv - Immunology 2022Quote: ... and Illumina linker addition to B cell heavy chain transcripts were performed using the human NEBNext Immune Sequencing Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... PolyA-selected libraries were made using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The ribosomal-depleted RNA was used to make the RNA-seq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760, NEB). These libraries were analysed through a Bioanalyzer for quality control ...
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bioRxiv - Synthetic Biology 2022Quote: ... NNN spacer to increase library diversity during sequencing (preadenylated oligos were prepared by 5’ DNA adenylation kit (E2610L) using thermostable 5’ App DNA/RNA ligase (M0319L, both from New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting PCR product was cloned into pBUE411 vector at the PmeI cut site by Gibson Assembly using NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs) and this produced the pBUE411_GRF-GIF construct (Figure 1a).
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bioRxiv - Plant Biology 2022Quote: ... was commercially synthesized (Tsingke) and ligated into the pCA2300-LbCpf1 vector through HindIII site using the T4 DNA ligase kit (NEB).
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bioRxiv - Zoology 2022Quote: ... Stranded cDNA libraries were created with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Fish were sequenced for 100 base pair reads on one lane of a NovaSeq 6000 (Illumina) ...
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bioRxiv - Zoology 2022Quote: ... The T7 reverse-complement sequence was annealed to the probe sets to allow transcription into RNA and biotinylation in a single reaction using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) followed by a Dnase treatment to avoid sample contamination by probe DNA during the capture ...
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bioRxiv - Zoology 2022Quote: ... DNA libraries were prepared using a NEBNext Ultra II DNA Library Prep Kit and NEBNext multiplex Oligos for Illumina (New England Biolabs). BLV-specific probes were used for the enrichment of DNA libraries with BLV sequence and the enriched libraries were PCR amplified using the primer set P5-P7 ...
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bioRxiv - Physiology 2022Quote: cDNA synthesis was completed using a NEB First Strand cDNA synthesis kit (M0368; New England Biolabs Canada, Whitby, ON, Canada). Briefly ...
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bioRxiv - Physiology 2022Quote: RNA-sequencing libraries were prepared by depleting eukaryotic ribosomal RNA with the NEBNext rRNA Depletion Kit prior to library synthesis with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and addition of multiplex oligos using the Unique Dual Index Primer Pairs Set 3 (NEB). Library quality control was performed using Qubit and Bioanalyzer 2100 ...
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bioRxiv - Physiology 2022Quote: ... RNA-seq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit coupled with Poly(A) mRNA Magnetic Isolation Module and NEBNext multiplex oligos for Illumina (New England Biolabs). Deep sequencing was performed on the NextSeq 500 sequencing system (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... The samples were sheared to around 450-bp fragments and the library constructed using the NEBNext UltraII DNA library kit (NEB), followed by qPCR for sample pooling and normalisation for the Illumina sequencing platform ...
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bioRxiv - Microbiology 2022Quote: ... Site-directed mutagenesis was used to introduce deletions and substitutions into expression vectors using the Q5 site directed mutagenesis kit (New England Biolabs) and according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 500 ng of total RNA was used to synthesize cDNA using the LunaScript RT SuperMix Kit (New England Biolabs, E3010L). The Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB). 1D library was prepared with the SQK-LSK108 kit (Oxford Nanopore ...
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bioRxiv - Microbiology 2022Quote: ... 12 µl template DNA was supplemented with the required reagents from the NEBNext Ultra II End Repair/dA Tailing kit (E7546S, NEB) and was first incubated at 20°C for 5 minutes and then at 65°C for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared and sequenced by the Edinburgh Clinical Research Facility using NEBNEXT Ultra II Directional RNA Library Prep kit (NEB) and Poly-A mRNA magnetic isolation module (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the eluates were treated with proteinase K for 16 h at 65°C before library synthesis using NEBNext Ultra II DNA library preparation kits (New England Biolabs). The samples were sequenced on HiSeq 2000 (Illumina) ...
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bioRxiv - Cell Biology 2022Quote: ... A biotin pull down was then performed on the size-selected fragments using streptavidin-coated beads before library preparation on the beads using the standard NEBNext Ultra II DNA library prep kit for Illumina (NEB) protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Barcoded stranded mRNA-seq libraries were prepared from 300 ng of high-quality total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-seq libraries were prepared from total RNA using polyA selection followed by the NEBNext Ultra II Directional RNA library kit workflow (New England Biolabs). Sequencing was performed on the Illumina HiSeq 2500 instrument ...
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bioRxiv - Molecular Biology 2024Quote: ... were PCR-amplified and subcloned into the Halo pcDNA5/FRT vector using Gibson Assembly Cloning Kit [New England Biotechnologies (NEB)] ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using primers containing the homology arm flanking the sgRNA region (replacing the BRD4 homology arm) and Gibson cloned (NEBuilder Hifi DNA assembly kit, NEB) into the same vector by digesting with MluI restriction enzyme.
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bioRxiv - Cell Biology 2024Quote: ... Total RNA libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, #E7760) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The CHD4-GFP mutant was cloned with selective primers (fwd, 5’-GGATGCTACAGGTGGAACCCTGCACCCCTA-3’; rev, 5’-GCCCAGGCCCGACGCCAT-3’) and a Q5 site-directed mutagenesis kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for DNA fragments were prepared based on the NEBNext Ultra II DNA library prep Kit for Illumina (NEB#E7645).
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bioRxiv - Molecular Biology 2024Quote: ... 25ul ChIP DNA or 10ng Input DNA were used to generate libraries using the NEBNext Ultra II Library Prep Kit for Illumina (NEB). Reactions were scaled down to half otherwise processing was according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2024Quote: ... The repair template was assembled and integrated into pCC1 using Gibson assembly (Gibson et al., 2008) (NEB Hifi assembly kit). Colony PCR was used to check the plasmids had the correct inserts (F-93/R-91 ...
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bioRxiv - Cancer Biology 2024Quote: ... The sequencing library was generated by combining equal amounts of purified PCR products and quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs). The primer 5’-ACACTCTTTCCCTACACGACGCTCTTCCGATCTTTGTGGAAAGGACGAAACACCG-3’ was used to sequence on Illumina platforms.
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) using 200-600ng total RNA as input ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was poly-A selected and RNA-Seq libraries were prepared using NEBNext Library Prep Kit for Illumina (NEB #E7760). A Miseq shallow sequencing run was performed after library preparation to balance the sample loading process on the deep sequencer ...
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bioRxiv - Cell Biology 2024Quote: ... domain from pmEGFP-N1-R-MCD(+0.2) plasmid (kind gift from Dr. Gregory Jedd) and cloned into XLone-GFP plasmid using Gibson assembly kit (NEB, E2611S). Two days after nucleofection ...
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bioRxiv - Developmental Biology 2024Quote: ... Complementary DNA (cDNA) was prepared from total RNA (5 μg) by reverse transcription using LunaScript® RT SuperMix Kit (NEB). qPCR reactions were performed using Power SYBR Green Master Mix (Thermo ...
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bioRxiv - Physiology 2024Quote: ... mRNA Magnetic Isolation Module was followed by library preparation using the NEBNext Ultra II RNA Directional Library Prep Kit for Illumina (New England BioLabs). Single read sequencing took place on a NextSeq 2000 System (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified DNA templates encoding 5’UTR of ATF4 mRNA were used for in vitro transcription of ATF4 reporter mRNA using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... and inserted into BamHI-HindIII-digested p413-PGPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S).
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bioRxiv - Cell Biology 2024Quote: ... Synthetic oligonucleotides encoding 3xFLAG were then ligated to the 5’ position of APEX2 sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs) to form the final vector pcDNA5/FRT/TO/3xFLAG-APEX2.
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared on beads using the Next Ultra II DNA Library Prep Kit for Illumina and Next Multiplex Oligos for Illumina (New England Biolabs) following instructions from the Arima-HiC+ for HiC (Arima Genomics) ...
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bioRxiv - Molecular Biology 2024Quote: ... 10ng of purified DNA (average size 250–300bp) was used to prepare sequencing libraries with the Next Ultra II Library Prep Kit for Illumina (New England Biolabs) using the application note for “Low input ChIP-seq” ...
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bioRxiv - Microbiology 2023Quote: ... the entire region of pET15b-His-sdeA except for the region encoding 1-199 aa of SdeA was amplified with primers 2649/2712 and then the fragment was self-ligated with a Gibson assembly kit (New England Biolabs). For construction of pmGFP-sdeAΔDUB ...
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bioRxiv - Neuroscience 2024Quote: ... DNA fragments were generated by PCR amplification and then fused with the backbones using NEBuilder HiFi DNA assembly kit (New England Biolabs). All plasmids were verified by sequencing (GeneWiz).
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bioRxiv - Cell Biology 2023Quote: ... The sequences were cloned into the pUASz1.0 vector (54) with the eGFP ORF at the C-terminal end of the Top3β ORF using a ligation kit (NEB). The UASz vector allows one to induce the constructs in any tissue ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified by phenol chloroform extraction and RNA was synthesized using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). 1 ug of starting linear DNA template was used and transcription was performed for 2hrs at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... or (Q71L for Arf1) and dominant negative (T27N for Arf6) or (T31N for Arf1) by using site-directed mutagenesis (SDM kit from NEB) kit ...